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Citations for Diagenode :
651 - 700 of 732 citations for DNA Damage 8 OHdG ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The sequencing libraries were generated using the Diagenode small RNA sequencing kit following the manufacturer’s protocol (Diagenode, Cat. No. C05030001). The libraries were sequenced on a NovaSeq6000 system (Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... using the Capture and Amplification by Tailing and Switching method (CATS RNA-seq Kit v2 x24, Diagenode Cat# C05010041, Leige, Belgium). 10ng RNA was used as input and the dephosphorylation step omitted to select for 3’-OH RNA species (miRNA) ...
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bioRxiv - Plant Biology 2022Quote: ... The crosslinking was performed by vacuum infiltration (~ –950 millibars) for 20 min and ChIP-qPCR were performed using Diagenode’s Universal Plant kit (Diagenode, Seraing, Belgium). The shared chromatin was immunoprecipitated using anti-GFP antibody (Abcam 290) ...
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bioRxiv - Neuroscience 2023Quote: ... and 20 ng of BH RNAs were used for small RNA library construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). D-Plex Single Indexes for Illumina - Set A (DiagenodeC05030010 ...
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bioRxiv - Cell Biology 2023Quote: ... a total of 10 ng of dsDNA was purified using the MicroPlex Library Preparation kit v3 (C05010001, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed once with PBS before adding cold lysis buffer iL1b (from the Auto iDeal ChIP-seq Kit for Transcription Factors, Diagenode C01010058). Asynchronously growing cells (collected by scraping ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed from 500 pg total RNA using D-Plex Small RNA-seq Kit for Illumina and Single Indexes for Illumina - Set B (Diagenode, C05030001). RIN aXer cell sorting was determined to be between 1.2 and 7.2 (Bioanalyzer 6000 ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... 50mg patient tissue RNA was extracted in an RNase free environment using RNA Extraction kit for Bioruptor Plus (Diagenode Cat# C20000010). The additional protocol listed for DNaseI treatment was followed for 10ug of extracted RNA per sample ...
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bioRxiv - Genomics 2024Quote: ... Then the bisulfite conversion was realized on the pool and the final libraries were amplified using MethylTaq Plus Master Mix (Diagenode kit). After a clean-up with AMPure XP Beads ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by immunostaining with Cas9 monoclonal antibody 4G10 (Diagenode cat#: C15200216; 1:200 dilution) with gentle shaking for 2h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... The following antibodies were used for western blot analysis: Cas9 (Diagenode C15310258, 1:1000) TCF4 (Abcam ab217668 ...
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The epigenomic landscape regulating organogenesis in human embryos linked to developmental disordersbioRxiv - Genomics 2019Quote: ... 1% SDS and protease inhibitor cocktail) and sonicated under prior optimised conditions (Diagenode Bioruptor). Sufficient sample was prepared to allow in parallel immunoprecipitation for H3K4me3 ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei diluted in 1 ml RIPA buffer were sonicated using Bioruptor (Diagenode, Denville, NJ) for 6 cycles of 5 minutes (60 seconds on/30 seconds off ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were bath-sonicated for 1 min at medium amplitude on a Bioruptor (Diagenode) to break bacterial chains and plated on plates with suitable antibiotics to select for transformants.
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bioRxiv - Biochemistry 2020Quote: Nucleosome assembly in the presence of Nap1 and ACF was carried out using a Chromatin assembly kit (C01030001) (Diagenode Inc. United States), following the manufacturer’s protocol with minor modifications to accommodate tracer DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP (Chromatin Immunoprecipitation) was performed using the reagents and protocol provided in the universal plant ChIP-Seq kit (Cat. No. C01010152; Diagenode, Ougree, Belgium) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... ribosome footprints between 26 and 34 bases were cut out for further processing with D-Plex Small RNA sequencing kit with unique molecular identifiers (UMI) (Diagenode, C05030001, C05030021) for library preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatins were sheared 4x 20 sec ON/1 min OFF with Bioruptor Pico sonicator (Diagenode), then diluted four times with dilution buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT) and sonicated for 3x 10 seconds using a Bioruptor Pico (Diagenode). Cells were then rotated at 4°C for 20 mins before the lysates were diluted with 500 µl of no salt buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... Loose chromatin was removed with 1% SDS prior to sonication on a Bioruptor™ (Diagenode). The immunoprecipitation step involved incubation with different antibodies (Flag M2 beads (sigma ...
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bioRxiv - Genomics 2023Quote: ... Crosslinked chromatin resuspended in 1 ml LB3 was then sonicated using a BioRuptor Pico (Diagenode) for 30 cycles of 30 seconds on/off ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling of Cas9 protein by monoclonal anti-mouse Cas9 antibody at 1:500 (4G10; Diagenode) was blocked in PBS Super Block (37580 ...
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bioRxiv - Genomics 2023Quote: ... 200,000 cells were centrifuged at 500g before proceeding with the nuclei isolation and transposition reaction using the ATAC-seq Kit from Diagenode (Diagenode Cat #C01080002). According to the results of the nuclei isolation optimization ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% SDS and sonicated to obtain approximately 200–500 bp chromatin fragments using a Bioruptor (Diagenode). Chromatin fragments were precleared with salmon sperm DNA (Trevigen ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM MgCl2,) and sonicated 10x with an interval of 30’’ on “medium” setting (Bioruptor300; Diagenode). Protein concentrations were measured using Quick Start™ Bradford 1x Dye Reagent from BIO RAD according to the manufacturers manual and equal amounts loaded for each lane.
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bioRxiv - Cancer Biology 2020Quote: ... using 1 µg antibody (anti-H3K4me1, #ab8895, Abcam; anti-H3K27ac, #ab4729, Abcam; anti-H3K4me3, #c15410003, Diagenode). 10% of the sheared chromatins were kept aside as input samples ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% SDS was then added followed by 3x 30sec sonication steps with a bioruptor sonicator (Diagenode) to solubilize the chromatin ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin of each replicate was incubated with 1 μg of antibody against H3K27ac (Diagenode, C15410196, RRID:AB_2637079) or 1 μg of antibody against ONECUT2 (Proteintech ...
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bioRxiv - Immunology 2024Quote: ... Beads were blocked with 1 µL of antibody (α-H3K27ac, Diagenode, C15410196; α-H3K4me3, Diagenode, C15410003) in antibody binding buffer for 3-4h at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was adjusted to about 10Lng μl−1 in 100Lμl and fragmented using a Bioruptor ultrasonicator (Diagenode) with 30Ls on/off for 30 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysate was transferred into reaction vessels and homogenized for 1 min using a Bioruptor® (Diagenode) set to medium power for 1 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was resuspended in 400µL of lysis buffer (1% SDS, 50mM Tris-HCl pH 7.5) and sonicated with the Pico Bioruptor (Diagenode) for 20 cycles (30sec ON/ 30sec OFF ...
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bioRxiv - Biochemistry 2024Quote: ... 1 x Halt Protease Inhibitor Cocktail) and water-bath sonicated (Diagenode; 25 cycles, 30 sec on and off). Sheard chromatin was diluted to achieve 0.1 % SDS and incubated with FOXA1 antibody (Novus Biologicals ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Genomics 2019Quote: ... at 0.3J/cm2, and then lysed (1% SDS, 50mM Tris pH7.5, 500 mM NaCl) and sonicated using a Bioruptor (Diagenode) (six cycles 30” on 45” off) ...
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bioRxiv - Cell Biology 2021Quote: ... Pelleted cells were resuspended in 1 ml 50 mM Tris pH 7.5 and sonicated in a Bioruptor Twin (Diagenode) on LOW 30 sec ON ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... 1:100) followed by sonication for 16 cycles (bursts and intervals of 30s each) with the Bioruptor® (Diagenode), which produced 200-1000 bp genomic DNA fragments ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... lysates were first sonicated for 3 cycles of 1 min pulse at 30 sec intervals in a Bioruptor (Diagenode) and then centrifuged at 4°C for 5 min at 1,000 g ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktails and sonicated for 1 min with the Bioruptor sonication device (Diagenode). Cell lysates were centrifuged at 13,000 × g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... the pellets were resuspended in 300 μL ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON - 60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed in 1 ml extraction buffer by three freeze-thaw cycles using liquid nitrogen and by sonication using a Bioruptor (Diagenode) with one 30 s high power pulse ...
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bioRxiv - Cell Biology 2021Quote: ... and for 15 minutes at 100°C in a proteinase K/elution buffer mix (1 µl of proteinase K in 100 µl DIB-buffer; Diagenode). Capture efficiency was determined by qPCR against spiked-in Lambda-DNA fragments in precapture and postcapture library samples ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % Triton X-100) supplemented with 1 x HALT in a 15 ml Bioruptor tube containing one scoop of sonication beads (Diagenode) and sonicated in Bioruptor Pico (Diagenode ...
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... cells were cross-linked with 1% paraformaldehyde for 15 minutes and Chromatins were sheared into an average 200–400 bp in length by sonication (Diagenode) and immunoprecipitated with following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... rested for 1 min on ice and sonicated (20 cycles: 15 seconds on/off; Bioruptor plus model UCD-300, Diagenode). Reduction and alkylation of disulfide bridges was performed by incubating the samples at 95 °C for 5 minutes ...
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bioRxiv - Genetics 2020Quote: S2 cells transfected with either mCherry-Nnk or Odj-mCherry for 72 hours were fixed with 1% paraformaldehyde for 10 minutes and sheared with Bioruptor sonicator (Diagenode) to obtain chromatin ...