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Citations for Greiner :
1 - 50 of 222 citations for MEK Kinase Kinase 1 MAP4K1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... In vitro kinase assays were performed in 384-well low volume plates (Greiner #784075) in 5µl reaction volume ...
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bioRxiv - Genomics 2020Quote: ... 1*106 undifferentiated cells/well were aggregated in 3ml 10μM Rho-Kinase inhibitor Y-27632 (RI) containing E8 medium on low attachment 6 well (Greiner Bio-one GmbH) plates on an orbital shaker (70rpm ...
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bioRxiv - Biochemistry 2020Quote: ... Serially diluted inhibitor and NanoBRET™ Kinase Tracer (as indicated in Table S8) were pipetted into white 384-well plates (Greiner 781 207) using an ECHO 555 acoustic dispenser (Labcyte) ...
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bioRxiv - Biochemistry 2020Quote: ... Serially diluted inhibitor and NanoBRET™ Kinase Tracer (as indicated in Table S8) were pipetted into white 384-well plates (Greiner 781 207) using an ECHO 550 acoustic dispenser (Labcyte) ...
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bioRxiv - Neuroscience 2021Quote: ... seeded (9,000 cells/well) onto poly-L-ornithine/ laminin-coated 384-well optical bottom plates containing the kinase inhibitors (Greiner Bio-One, Kremsmünster, Austria) and cultured in neuronal differentiation medium for two weeks (Fig ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:1000 in 400 µl of antibody solution (2.5% BSA, 0.05% Triton X-100, 1×PBS) in a 12-well culture plate (Greiner) and incubated overnight for at least 15 h at RT ...
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bioRxiv - Immunology 2024Quote: ... BLI assays to detect tyrosine sulfation by binding antibodies with anti-sulfotyrosine antibodies were performed using polypropylene black 384-well microplate (Greiner) at 30°C in octet buffer (0.05% Tween in PBS) ...
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bioRxiv - Biochemistry 2024Quote: ... Antibody titration series was performed in a half-area 96-well plate (Greiner) starting at a 2x concentration of 20 μg/ml and diluting 1:3 with DMEM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: 200,000 mouse EpCAM+ primary cells and 200,000 CCL206 fibroblasts (n=4 independent isolations) were seeded in 1 ml Matrigel diluted 1:1 in DMEM containing 10% FBS in 6-well plates (Greiner Bio-One, The Netherlands). 12.000 or 30.000 nylon reference microfibers were mixed with Matrigel and cells prior to seeding ...
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bioRxiv - Microbiology 2022Quote: ... 50 μL/well of antibody was added to a 96-well plate (Greiner Bio-One 655096). A master stock of microspheres was created in PBS/1% BSA to allow 2000 beads/analyte/well ...
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bioRxiv - Biochemistry 2020Quote: Soluble antibody fragments (scFv) were produced in 96-well polypropylene MTPs (U96 PP, Greiner Bio-One) as described before 55,71 ...
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bioRxiv - Immunology 2020Quote: ... The virus-monoclonal antibody mixture was added (2 mL) to Vero cells in a 6-well plate (Greiner). After infecting the cells for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... 500 ng of each antibody was coated onto high-binding 96-well polystyrene microplates (#07000128; Greiner Bio-One) at 100 μl per well and incubated overnight at room temperature (RT) ...
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bioRxiv - Cancer Biology 2020Quote: HMEC-1 cells or HMEC-1 cells overexpressing xCT or SLC1A3 were seeded into 24-well plates (Greiner Bio-One) at a density of 6.5 × 103 cells per well in 0.5 mL medium ...
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bioRxiv - Cancer Biology 2020Quote: ... The labeled tumor cells were mixed in a 1:1 ratio and 4×106 cells were seeded per 10 cm dish (Greiner). The tumor cell mix was subsequently challenged three times for 24 hours with either MART-1 T cells or control T cells at a 1:8 ratio ...
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bioRxiv - Cell Biology 2021Quote: ... 1% L-glutamine and 1% penicillin–streptomycin without phenol red (ThermoFischer) in glass-bottom CELLview culture dishes (Greiner Bio-One) for 24h and serum starved for 32h ...
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bioRxiv - Bioengineering 2021Quote: ... directly seeded on (10 µg/ml) anti CD43-biotin antibody coated plastic-bottom 96 well plates (Greiner Bio-one), stored for 30 min at 4°C and fixed with 4% paraformaldehyde (Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... progress was tracked by the change in absorbance at 340 nm by the formation or consumption of NADPH (ε340= 6,220 M-1 cm-1) in UV-transparent 96 well plates (Greiner, Sigma-Aldrich) measured using a SpectraMax Plus microplate reader (Molecular Devices).
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bioRxiv - Molecular Biology 2021Quote: ... Purified protein with 25 mg/ml concentration was mixed with sparse matrix crystallization screening conditions in a 1:1 volumetric ratio by using 72 well Terasaki crystallization plates (Greiner-Bio, Germany). The mixture was sealed with 100% paraffin oil to slow down the vapor diffusion (Tekkim Kimya ...
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bioRxiv - Immunology 2021Quote: ... Cells were mixed (ratio 1:1) and plated at 8×103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with serially diluted mAb or sera for 15 minutes at room temperature and added to S-Fuse cells ...
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bioRxiv - Microbiology 2021Quote: ... Cells were mixed (ratio 1:1) and plated at 4×103 per well in a μClear 96-well plate (Greiner Bio-One). The following day ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cultures were then inoculated into DM29-fructose (5 g L- 1) and DM29-glucose (5 g L-1) media in a 96-well plate (Greiner Bio-One) to an initial OD600 of 0.05 ...
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bioRxiv - Microbiology 2020Quote: ... with the modification of mixing undiluted samples 1:1 with 2 mM TCEP in wells of a white 96-well plate (Greiner Bio-one) prior to use ...
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bioRxiv - Microbiology 2022Quote: ... Cells were mixed (ratio 1:1) and plated at 8×103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with mAb ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with Fibronectin (25 μg ml−1) was incubated at 1 hour at 37 °C to polymerize it in the inserts (8 μm ThinCert; Greiner bio-one). Inserts were then inverted and cells were seeded on the opposite side of the filter and allowed to attach to the matrix for 4h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... were mixed (ratio 1:1) and plated at a density of 8 × 103 per well in a μClear 96-well plate (Greiner Bio-One) as previously described (Buchrieser et al. ...
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bioRxiv - Immunology 2021Quote: ... Cells were mixed (ratio 1:1) and plated at 8×103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with monoclonal antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were mixed (ratio 1:1) and plated at 8×103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with mAb ...
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bioRxiv - Microbiology 2022Quote: ... Cells were mixed (ratio 1:1) and plated at 8 × 103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with serially diluted monoclonal antibodies or sera for 15 min at room temperature and added to S-Fuse cells ...
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bioRxiv - Cancer Biology 2023Quote: ... 5×104 tumor cells were co-cultured with 5×104 T cells (1:1 ratio) in a round bottom 96 well plate (Greiner, M9436-100EA) for 6 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Product formation was monitored spectrophotometrically at 380 nm (ε = 15,800 M−1 cm−1) in individual 96-well plates (Greiner Bio-One) wells using a Biotek Synergy H1 multimode plate reader (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were mixed (ratio 1:1) and plated at 8 × 103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with serially diluted monoclonal antibodies or sera for 15 min at room temperature and added to S-Fuse cells ...
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bioRxiv - Immunology 2020Quote: Total and NP-specific antibody levels were measured by ELISA on half-area 96-well plates (Greiner Bio-One, 675061). Wells were coated overnight at +4°C with capture antibodies (2 μg/mL ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) in a 384-well microplate (Greiner) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 20,000 cells were infected with HIV-1* or HIV-1*CA14SiR in a 96-well v-bottom microplate (Greiner Bio-one, cat. #650161) in a volume of 40 µl RPMI and transferred at 22 h p.i ...
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bioRxiv - Molecular Biology 2022Quote: Assays were performed as previously described where 80 nM UPF1-HD was pre-bound to 1 µM substrate29 but (Table S3) supplemented with 1 mM DTT and using clear 96 well half area plates (Greiner Bio-one 675101). All experiments were performed on a CLARIOstar Plus plate reader at 37°C with 1 mM ATP (BMG Labtech).
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bioRxiv - Biophysics 2023Quote: ... Equal volumes of crystallization conditions were mixed with 0.83 μL of 30 mg/mL lysozyme solution (1:1 v/v) in a 72-well Terasaki plate (Cat#654180, Greiner Bio-One, Austria). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... Two μL of antibody diluted in PBS (pH 7.4) was coated on a high-binding 96-well polystyrene microplate (Greiner Bio-One) with 100 μL per well and incubated overnight at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: ... the effector cells (1 × 104 cells) were cocultured with the target cells (1 × 104 cells) in a 384-well white plate (Greiner Bio-One, Kremsmünster, Austria). The cells were then incubated for 24 hours at 37°C in 5% CO2 and the luciferase activity was measured using the ONE-Glo™ luciferase assay (Promega ...
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bioRxiv - Molecular Biology 2023Quote: The crystallization screening of N-terminal decahistidine cleaved TRAF6 was performed using the sitting-drop microbatch under oil method against ∼3000 commercially available sparse matrix crystallization screening conditions in a 1:1 volumetric ratio in 72-Terasaki plates (Greiner Bio-One, Kremsmünster, Austria) as described in Ertem 2021 et al ...
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bioRxiv - Biophysics 2023Quote: ... The purified wild-type and Val120Thr mutant CbFDH protein were mixed with ∼3500 commercially available sparse matrix crystallization screening conditions in a 1:1 volumetric ratio in 72-Terasaki plates (Greiner Bio-One, Kremsmünster, Austria). The mixtures were covered with 16.6 Μl 100% paraffin oil (Tekkim Kimya ...
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bioRxiv - Microbiology 2021Quote: ... 1 mm height) and stamped into 24-well SensoPlates (Greiner). Positions were manually selected to track the fate of both cell types in the same field of view (note that cell types were unevenly distributed on the same pad) ...
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bioRxiv - Physiology 2023Quote: ... whole heparinized blood was diluted 1:1 with PBS in sterile conditions and transferred to a Leucosep™ tube (Greiner Bio-One, cat no. GREI163290_500). Following centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... in a 1:1 ratio and then equilibrating the mixture against a 150-μL reservoir in 96-well plates (Greiner Bio-one, GmbH, Frichenhausen, Germany). The crystallization plates were subsequently maintained at 18 °C in an automated RockImager1000 (Formulatrix ...
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bioRxiv - Immunology 2021Quote: ... HLA bound radioactivity was determined by capturing MHC/peptide complexes on W6/32 antibody coated Lumitrac 600 plates (Greiner Bioone, Frickenhausen, Germany). Bound cpm was measured using the TopCount (Packard Instrument Co. ...
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bioRxiv - Neuroscience 2023Quote: ... to give the optimised antibody concentration when added to 10 μL of a 10 % lysate concentration in a 384-well ProxiPlate (Greiner Bio-One). Following incubation for 3 h on an orbital shaker (250 rpm) ...
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bioRxiv - Immunology 2023Quote: ... Serum Ym1-specific immunoglobulin M (IgM) and OVA-specific immunoglobulin G1 (IgG1) antibody levels were determined in flat-bottom 96-well plates (Greiner Bio-One), coated overnight with 0.1 mg/ml soluble Ym1 in PBS or with 0.1 mg/ml OVA (Worthington ...
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bioRxiv - Immunology 2021Quote: ... 1 ml was transferred into a 2 ml cryovial (Greiner, Belgium). The vials were frozen using a Mr ...
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bioRxiv - Cancer Biology 2020Quote: ... Plate 1 dome per well in 24-well plate (Greiner CELLSTAR), with 50 μL of cells/Matrigel suspension per dome ...
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bioRxiv - Molecular Biology 2021Quote: ... The droplets were collected in a 1 ml collection tip (Greiner) inserted to the outlet ...