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Citations for Stemcell Technologies :
1 - 50 of 162 citations for Vitamin D Depleted Serum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The serum was then depleted from CD45+ cells using magnetic separation beads (StemCell Technologies), and the remaining samples were split into 2 fractions ...
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bioRxiv - Molecular Biology 2023Quote: ... NeuroCult SM1 Without Vitamin A (StemCell Technologies), N-2 supplement (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... NeuroCult SM1 Without Vitamin A (StemCell Technologies), N-2 supplement (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... 1 × NeuroCult SM1 without vitamin A (StemCell Technologies), and 1 × Antibiotic-Antimycotic ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with NeuroCult SM1 (without vitamin A; StemCell Technologies, #05731) and N2 supplement-A (StemCell Technologies ...
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bioRxiv - Immunology 2022Quote: ... and depleted of lineage-positive cells by magnetic columns (Stem cell technologies) to enrich for HSPC ...
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bioRxiv - Immunology 2022Quote: ... CD3+ cells were depleted using the negative selection cocktail RosetteSep™ (STEMCELL Technologies Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Dead cells were depleted with EasySepTM Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, #17899). Cells in suspension were stained with ReadyProbes Cell Viability Imaging Kit (Hoechst 33342 and Propidium Iodide ...
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bioRxiv - Immunology 2019Quote: ... Antibody-bound cells were depleted with Rapidsphere streptavidin magnetic beads according to the manufacturer’s instructions (STEMCELL Technologies).
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bioRxiv - Immunology 2022Quote: ... Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies), irradiated (20 Gy ...
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bioRxiv - Cell Biology 2024Quote: ... bone marrow cells were further lineage depleted using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
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bioRxiv - Systems Biology 2020Quote: ... CD66b and glycophorin A were depleted from PBMCs using magnetic sorting (Human Pan-DC Pre-Enrichment Kit, StemCell Technologies). pDCs were then sorted on a FACS Vantage instrument (BD Biosciences) ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using the EasySep Biotin Positive Selection Kit (Stemcell Technologies) with anti-human/mouse β7 integrin antibody (FIB504 ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and residual natural killer cells were depleted using 100 μL/mL of the CD56 Positive Selection Cocktail (STEMCELL Technologies). Monocytes (5ξ106/vial ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Genetics 2019Quote: ... lineage positive cells were depleted by negative selection with the StemSep Human Hematopoietic Progenitor Cell Enrichment Kit (StemCell Technologies, 14056) and Anti-Human CD41 TAC (Stem Cell ...
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bioRxiv - Bioengineering 2022Quote: ... Antibody-bound cells were depleted with streptavidin RapidSphere™ magnetic beads according to the manufacturer’s instructions (STEMCELL Technologies; Vancouver, Canada). Cells with >90% purity were used for experiments ...
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bioRxiv - Cell Biology 2019Quote: ... Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+) kit (STEMCELL Technologies). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... the dead cells were first removed with a Dead Cell Removal Kit and then the NK cells were depleted using the EasySep Human CD56 Positive Selection Kit (Stemcell Technologies). The control 221 cells were kept in the same culture conditions but were not exposed to NK cells ...
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bioRxiv - Immunology 2023Quote: ... CD8α+ cells were purified from CD4 and CD8β depleted T-IEL and lymph node T cells using the CD8α Positive Selection Kit (Stemcell Technologies). Cells were resuspended in RPMI + 1% FBS + L-Glutamine + Pen/Strep at a concentration of 1x106 cells/ml and stimulated with 10 µM of pervanadate ...
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bioRxiv - Immunology 2021Quote: ... and rat serum (Stemcell Technologies), followed by the anti-phosphoSTAT3 (Tyr705 ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were stained and magnetically isolated from the lineage-depleted cell suspension using the EasySep mouse CD117 (c-Kit) positive selection kit (Stem Cell Technologies), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... we thawed frozen viable breast cancer pleural effusions or ascites from patients and depleted dead cells with EasySep™ Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies) and CD45+ cells with EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Lineage-depleted bone marrow was then stained with an antibody cocktail comprised of a lineage cocktail (BioLegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). 2.5-3.0 × 106 lineage-depleted bone marrow was then washed in PBS and then resuspended in 135 μL Buffer T to which 15 μL of Dre mRNA (at 1 μg/μL ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Cancer Biology 2022Quote: ... using base medium (1:1 ratio of DMEM/F12 Glutamax/Neurobasal medium [Gibco], 2% B27 supplement without vitamin A [Gibco], 10% BIT 9500 [StemCell Technologies], and 1 mM glutamine [Gibco]) supplemented with 20ng/ml EGF (Chemicon) ...
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bioRxiv - Neuroscience 2023Quote: ... serum-free media (mTeSR, Stemcell Technologies). For neuronal induction ...
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bioRxiv - Neuroscience 2023Quote: ... serum-free media (mTeSR, STEMCELL Technologies). Neuronal induction was performed using a modification of a previously described protocol.44 Cells were dissociated with Accutase (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... CD34+ cells were enriched by EasySep™ from the light-density fraction of Lymphoprep™ or RosetteSep™-depleted CD11b+;CD3+;CD19+ cells (STEMCELL Technologies) and then used after cryopreservation in DMSO and fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: CD4+ and CD8+ T cell subsets were depleted from PBMC using magnetic bead-based CD4 and CD8 negative selection kits (Stem Cell Technologies, Vancouver, Canada). The cell separations were performed according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... CD45 positive cells were depleted from tumor cell digests using the EasySep ™ Mouse streptavidin Rapidspheres™ Isolation Kit (Stem Cell Technologies, Catalog #19860) and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... serum-free medium mTESR-1 (StemCell technologies).
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bioRxiv - Neuroscience 2024Quote: ... BIT 9500 serum substitute (10%; StemCell Technologies), GlutaMAX (1X ...
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bioRxiv - Cell Biology 2020Quote: ... in StemSpan SFEM serum-free media (StemCell Technologies) with 50 ng/mL recombinant murine sKITL (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: ... bovine serum albumin (BSA; 1 %; Stem Cell Technologies), mSCF (10 ng/mL) ...
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bioRxiv - Immunology 2021Quote: ... and/or Normal Rat Serum (STEMCELL Technologies: 13551). Flow cytometry was performed on LSR Fortessa (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... serum-free mTeSR™1 medium (STEMCELL Technologies). Cells were passaged ∼1:10 at 80% confluency using ReLeSR (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... serum-free mTeSR™1 medium (STEMCELL Technologies). Cells were passaged ∼1:10 at 80% confluency using ReLeSR (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... StemSpan Serum-Free Expansion Medium (SFEM, StemCell Technologies) was supplemented with 2 × 102 IU/mL Penicillin-Streptomycin ...
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bioRxiv - Immunology 2022Quote: ... and/or rat serum (2%; Stem Cell Technologies) for 5min on ice prior to adding fluorescent antibodies (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of 2% rat serum (Stemcell technologies) was added to each well for 15 minutes at room temperature and proceeded to further staining without a washing step.
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bioRxiv - Immunology 2021Quote: ... Lymphoprep (d = 1.077 g mL−1, StemCell Technologies ™, Canada) was underlaid and PBMC were isolated by density gradient centrifugation ...
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bioRxiv - Bioengineering 2021Quote: ... with 20% BIT 9500 serum substitute (STEMCELL Technologies, 09500) 0.05% LDL(STEMCELL Technologies ...
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bioRxiv - Genetics 2022Quote: ... serum-free mTeSR plus medium (Stemcell Tech, 100-0276) according to manufacturer’s instructions and detached using 5mM EDTA/PBS or accutase ...
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bioRxiv - Genomics 2022Quote: ... serum-free mTeSR™1 medium (85850, STEMCELL Technologies). Cells were passaged ∼1:10 at 80% confluency using EDTA 0.5mM (15575020 ...
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bioRxiv - Cell Biology 2020Quote: ... Erythroblasts were cultured in a serum-free mononuclear cell (MNC) medium containing the following cytokines diluted in Stem Span Serum Free Expansion Medium (Stem Cell Technologies, CA): 40□ng/mL insulin-like growth factor 1 (IGF1) ...
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Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancerbioRxiv - Cell Biology 2022Quote: ... in serum-free mTeSR stem cell medium (Stem Cell Technologies). ESCs were characterized based on morphology to grow in flat and tightly-packed colonies with well-defined borders comprised of cells with high nuclear-to-cytoplasmic ratios and the ability to expand and differentiate ...