Labshake search
Citations for Stemcell Technologies :
1 - 50 of 378 citations for Round Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... 8000 iPSCs were seeded in 96-well round-bottom plates with APEL2 medium (05271, STEMCELL Technologies) containing 100 ng/mL human Stem Cell Factor (SCF) ...
-
bioRxiv - Neuroscience 2023Quote: ... 9000 cells were plated per well in a 96-well round-bottom ultra-low attachment plate containing EB seeding medium (Stem Cell Technologies). At day 2 and day 4 ...
-
bioRxiv - Immunology 2024Quote: ... gated for cKit+Sca1+CD11b−IL7Rα− cells and then single cell index sorted into 96-well round-bottom plates containing StemSpan™ SFEM II (Stemcell Technologies) supplemented with 50 ng/mL stem cell factor (in-house ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were then seeded into 96-well round bottom plates (cell density at 100,000 (100k) cells/100 μL) and prestimulated in StemSpanTM SFEM (Stem Cell Technologies #09650) supplemented with StemSpan™ CC100 (Stem Cell Technologies #02690) ...
-
bioRxiv - Bioengineering 2023Quote: ... wells of the U-bottom 96 well plate were treated with an anti-adherence rinsing solution (STEMCELL Technologies 07010) and centrifuged at 1300g for 5 min ...
-
bioRxiv - Neuroscience 2023Quote: ... iPSCs were evenly distributed into a flat-bottom 96-well plate in mTeSR1 media (StemCell Technologies) and 10 µM Y-27632 (StemCell Technologies) ...
-
bioRxiv - Microbiology 2022Quote: ... were seeded into the wells of 96-well plates (Costar, flat bottom) and maintained in Pneuma-Cult Expansion medium (PC-Ex) (STEMCELL TECHNOLOGIES) till confluency ...
-
bioRxiv - Cancer Biology 2024Quote: ... detached WTC-1185 human iPSC cells were transferred to an ultra-low attachment U-bottom 96-well plate (20K cells/well) and cultured in mTeSR Plus media (StemCell Technologies, 5825) supplemented with 10 μM Y-27632 (StemCell Technologies ...
-
bioRxiv - Cell Biology 2023Quote: ... For time first division Single cell LIN-CD34+ CD38- CD9+ or CD9- cells sorted in to the round bottomed 96 well plates and culture in SFEM (StemCell Technologies), supplemented with SCF (10ng/ml) ...
-
bioRxiv - Neuroscience 2019Quote: ... The supernatant was subsequently plated in a 96-well V-bottom at a density of 20,000 cells with Neural Induction Medium (StemCell Technologies) supplemented with dual SMAD inhibitors SB321542 (10 μM ...
-
bioRxiv - Neuroscience 2022Quote: ... the dissociated cells were transferred to individual low-adherence V-bottom 96-well plates (S-Bio Prime) in 150μl TeSR5/6 media (StemCell Technologies®) with 50μM Rock inhibitor Y-27632 (Tocris BioScience) ...
-
bioRxiv - Cancer Biology 2022Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
-
bioRxiv - Cancer Biology 2024Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were plated into 96 well flat bottom plates in growth medium supplemented with 10 µM of ROCK inhibitor (Stem Cell Technologies). Cells were plated at low density (2,500 cells/well ...
-
bioRxiv - Bioengineering 2023Quote: ... and plated at 12,000 cells per well into Cultrex (R&D Systems 343400502)-coated 96-well plates with mTeSR media (StemCell Technologies) containing ROCK inhibitor Y-27632 (10 uM ...
-
bioRxiv - Neuroscience 2021Quote: ... 2.5 mL of cell suspension was transferred to a sterile 5 mL round-bottom flow cytometry tube and placed in the EasySep magnet (STEMCELL Technologies) for 5 min ...
-
bioRxiv - Bioengineering 2023Quote: ... and re-plated at 12,000 cells per well into Cultrex-coated 96-well plates with mTeSR media (StemCell Technologies) containing ROCK inhibitor Y-27632 (10 uM ...
-
bioRxiv - Bioengineering 2024Quote: ... each well of the 96 well plate was first coated with 45 μL 2.25% Type I Bovine Collagen Solution (StemCell Technologies) for 1 hour at 37 C ...
-
Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... setting the instrument to deposit one cell into each well in a 96-well plate containing working medium with 10% CloneR cloning supplement (Stemcell Technologies). Filled plates were transferred to the 37°C incubator and left undisturbed for 48 hours after sorting ...
-
bioRxiv - Cell Biology 2021Quote: ... iPSCs were seeded at 9 × 103 cells per well onto 96-well ultralow attachment plates for embryoid body (EB) formation and grown in EB formation media (StemCell Technologies). At day 5 ...
-
bioRxiv - Microbiology 2021Quote: ... iPSCs were plated at 9,000 cells per well onto 96-well ultralow attachment plates for embryoid body (EB) formation and grown in EB formation media (StemCell Technologies). At day 5 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and were plated in a concentration of 9,000 cells/well into an ultra-low-binding 96-well plate in mTeSR™1 medium (Stemcell technologies) containing Rho-associated protein kinase (ROCK ...
-
bioRxiv - Pathology 2022Quote: ... and plated onto low attachment V-type 96-well plate at 20 000 cells per well or AggreWell800 plate (Stemcell Technologies) to form aggregates ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cells were cultured for 72 hours in 96-well plates (2X105-5X105 cells per well) containing StemPro (Stem Cell Technologies) media supplemented with human IL-6 ...
-
bioRxiv - Bioengineering 2020Quote: ... to sort single cells into Matrigel-coated 96-well plates with each well containing 150 μl of mTeSR1 supplemented with CloneR (STEMCELL Technologies, #05888). Following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: Patient-derived PB and/or BM MNC were plated at 4 x 104 cells per well in 96-well plates in 200 µL of RPMI supplemented with 10% fetal bovine serum and 10X StemSpan (StemCell Technologies #02691) (Pardanani et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... and plated at 10,000 cells per well in PDL-coated 96-well plates in BrainPhys Neuronal Medium (BrainPhys (STEMCELL Technologies; Cat. No. 05790) as the base ...
-
bioRxiv - Cell Biology 2023Quote: ... spectra were selected and seeded as single cell per well in a Geltrex coated 96-well plate containing E8 completed medium supplemented with Clone-R (STEMCELL Technologies, Vancouver, Canada). Plates were spun down at 400rcf for 1 minute to increase single cell attachment and were expanded at 37 °C in a humified atmosphere in the presence of 5% v/v CO2 until colonies were formed that could be used to generate a culturable cell line.
-
bioRxiv - Molecular Biology 2020Quote: ... and live CD34+ cells were sorted into 96-well plates containing MethoCult Optimum (STEMCELL Technologies, Vancouver, Canada). After 12–16d ...
-
bioRxiv - Neuroscience 2023Quote: ... Single GFP-positive cells were sorted in a 96-well plate with mTeSR plus CloneR (StemCell Technologies). 14 days after plating ...
-
bioRxiv - Genetics 2023Quote: ... the top 40% of ATTO-550 positive cells were sorted as single-cells into individual wells of two 96-well plates with different combination of CellAdhere Laminin-521 (STEMCELL Technologies, Cat#77004, (2 μg/cm2)) or Vitronectin (0.5 μg/cm2) ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were cultured into 96-well low-attachment plates containing NeuroCult Basal Media (Mouse and Rat; STEMCELL Technologies) with Proliferation Supplement (STEMCELL Technologies) ...
-
bioRxiv - Bioengineering 2023Quote: ... reactions were set up in 96 wells plates using the hPSC Genetic Analysis Kit (STEMCELL technologies, cat. 07550) as per manufacturer instructions with triplicates for each sample ...
-
bioRxiv - Neuroscience 2023Quote: ... these were cultured into either 96-well low-attachment plates containing Neurosphere Basal Medium containing Proliferation Supplement (STEMCELL Technologies) 20ng/mL hrEGF (STEMCELL Technologies) ...
-
bioRxiv - Cell Biology 2023Quote: 5,000 HSPCs were sorted directly into TC-treated 96-well plates (Falcon) containing StemSpan™ SFEM II (Stemcell Technologies) with Pen-Strep (Fisher Scientific ...
-
bioRxiv - Genomics 2023Quote: ... were index-sorted into 96-well plates (Supp. Table 14 - “HSC sc-CFU” panel) containing StemSpan SFEM (Stemcell Technologies) supplemented with penicillin/streptomycin (10U/ml penicillin ...
-
bioRxiv - Immunology 2023Quote: ... EL08.1D2 stromal cells were grown to a confluent monolayer on flat-bottomed 96 well ImageLock plates (Essen Bioscience) pre-coated with 0.1% gelatin (Stemcell Technologies). For imaging primary cells ...
-
bioRxiv - Microbiology 2021Quote: ... the hiPSCs were allowed to self-aggregate in 96-well plates for five days using Neural induction medium (Stem cell technologies) as described earlier (Gabriel et al. ...
-
bioRxiv - Cancer Biology 2022Quote: ... were directly sorted into a 96-well tissue culture plate containing 100 μL of differentiation media: StemSpan (Catalog #09650, StemCell Technologies), 1% Penicillin+Streptomycin ...
-
bioRxiv - Cancer Biology 2022Quote: CD34-positive and Lin-negative cells from PBMC were single-cell sorted into 96-well plates containing 100 μL of free medium consisting of StemSpam SFEMII (Stemcell Technologies) with human cytokines SCF ...
-
bioRxiv - Neuroscience 2023Quote: ... sorted into 96-well plates using a WOLF benchtop microfluidic cell sorter (Nanocellect) in mTeSR Plus and CloneR (Stemcell Technologies, 05888). After thirteen days ...
-
bioRxiv - Neuroscience 2022Quote: ... EGFP positive cells with a moderate GFP signal were seeded to Matrigel-coated 96-wells in mTeSR Plus medium supplemented for 3 days with CloneR (both StemCell Technologies). After 3 days ...
-
bioRxiv - Developmental Biology 2023Quote: ... they were collected and moved to a new 96-well plate containing 150 µl of induction media (Cerebral organoid kit, cat. n. 08570, StemCell Technologies) per well ...
-
bioRxiv - Cell Biology 2023Quote: Luminal or luminal progenitor cells were isolated from mouse mammary glands and sorted in 96-well plate filled with 5% FBS EpiCULT full medium (STEMCELL Technologies). 500 luminal cells or 200 luminal progenitors were then transferred to a 48-well plate previously seeded with 10,000 NIH3T3 irradiated feeder cells per well and incubated at 37°C ...
-
bioRxiv - Bioengineering 2023Quote: ... we single cell sorted ATTO550 positive cells for monoclonal expansion in 96-well plates containing their corresponding dTMP-supplemented culture media (hiPSC additionally supplemented with 10% Clone R (StemCell Technologies)) ...
-
bioRxiv - Cell Biology 2023Quote: For cell growth assay 100 LIN-CD34+CD38- CD9+ or CD9- cells were sorted and seeded in 96 well plate and culture in StemSpan Serum –Free Expansion Medium (SFEM, StemCell Technologies) supplemented with 100u/ml penicillin (Hyclone ...
-
bioRxiv - Bioengineering 2022Quote: ... were cultured in clear 96-well Greiner COC plates which were plasma treated and coated with 0.1% gelatin (STEMCELL Technologies, Vancouver, Canada). iCell cardiomyocytes plating medium from the iCell Cardiomyocytes Media Kit was used to plate the cardiomyocytes at 60,000 cells/well and then exchanged with iCell Cardiomyocytes maintenance medium after 24 hours ...
-
bioRxiv - Cancer Biology 2019Quote: Single cells were FACS-isolated into flat bottomed 96-well plates containing 100 μl of MethoCultTM H4435 Enriched (StemCell Technologies Cat#04435). Colonies were visually inspected and classified 11-14 days after plating ...
-
bioRxiv - Developmental Biology 2021Quote: ... Scleral punches were initially placed into wells of a 96 well culture plate with 50 μl of N2 medium [Ham’s F-12/DMEM containing 1x N2 supplement (Stem Cell Technologies, Vancouver, BC) until all sclera were obtained ...
-
bioRxiv - Immunology 2019Quote: ... A total of 649 IgGpos/OVApos hybridoma cells were individually deposited into 96-well plates containing Medium E (StemCell Technologies, Vancouver, BC, Canada). Supernatants were screened by ELISA against OVA seven days after sorting ...