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Citations for Stemcell Technologies :
1 - 50 of 695 citations for Recombinant Mouse Cd4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CD4+ T cells were isolated using EasySep Mouse CD4+ T Cell Isolation Kit (Stemcell technologies) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: CD4+ cells were isolated from freshly dissociated mouse spleen using the EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, 19852). CD4+ cells were then immediately used for bulk ATAC-seq ...
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bioRxiv - Cancer Biology 2020Quote: CD4+ T cells were isolated using the EasySep Mouse CD4+ T-cell isolation kit (Stemcell Technologies). The CD4+ T cells were then added in a 1:1 ratio to cultured CMT167 cells ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... CD4+ cells were separately isolated using mouse CD4+ T-cell negative selection protocol (EasySep™ Mouse CD4+ T-cell Enrichment Kit, Stem Cell Technologies) to a purity of > 90% according to the manufactureŕs instructions ...
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bioRxiv - Immunology 2022Quote: Splenocyte-derived CD4+ T cells were isolated using Easysep Mouse CD4+ T cell isolation kit (19852, STEMCELL Technologies), and then 2e5 cells/well were plated in a 96 well plate ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells were isolated with EasySep Mouse Naïve CD4+ T Cell Isolation Kit (STEMCELL Technologies Inc) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...
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bioRxiv - Immunology 2024Quote: ... Effector CD4+ T cells were isolated by magnetic enrichment using EasySep mouse CD4+ T cell Isolation Kit (STEMCELL Technologies) with addition of 10 µL of biotinylated anti-CD62L (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... and a mouse naïve CD4+ T cell Isolation kit (STEMCELL Technologies). The cell culture plates were pre-coated with anti-mouse CD3 antibody (Biolegend ...
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bioRxiv - Genomics 2020Quote: ... CD4 and CD8 T cells were isolated from a mouse using CD4 and CD8 negative selection kits respectively (StemCell Technologies) and were labelled with the caged strand hybridized to the Ab-oligo conjugate ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies, Canada). Cd4-/- mice were adoptively co-transferred intravenously (i.v. ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies). CD4+ T cells (4.0 × 105/well ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were then sorted using an EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852). The cells were incubated at 1×106 cells/mL in skew media in anti-CD28 (InVivoMab ...
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bioRxiv - Immunology 2023Quote: ... Splenic naïve OT II-CD4+ T cells were isolated using Mouse EasySep Naïve CD4+ T cell Isolation kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were first enriched for OT-II CD4+ T cells using EasySep™ mouse CD4+ T cell isolation kit (StemCell Technologies), incubated with FcR block and stained with the following fluorescence-conjugated antibodies ...
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bioRxiv - Immunology 2022Quote: Splenic naïve CD4+ T cells were negatively selected using an EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naïve T cells were resuspended in supplemented RPMI 1640 medium with penicillin (100 U/ml) ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from spleens and lymph nodes using the EasySep mouse CD4+ T cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and purified CD4+ T cells were obtained by positive selection with the “EasySep Mouse CD4 Positive Selection II” kit (StemCell Technologies). Then ...
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bioRxiv - Immunology 2024Quote: ... n=1 female) mice were prepared by depleting CD4+ cells from thymocytes using the EasySepTM Mouse CD4 Positive Selection Kit II (STEMCELL Technologies). Cells were then incubated with Fixable Viability Dye eFluor™ 780 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from splenocytes by the EasySep Mouse CD4+ T-cell Isolation Kit (#19852, STEMCELL Technologies, Vancouver, Canada) were used to evaluate the capability to differentiate into different T cell subsets as previously described [18,19,20] ...
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bioRxiv - Immunology 2019Quote: The EasySep mouse naïve CD4+ T cell isolation kit (Stemcell Technologies, 19765A) was used to isolate T cells from the spleens of untreated mice ...
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bioRxiv - Neuroscience 2023Quote: ... An EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852) was used per manufacturer’s protocol to sort naïve CD4+ T cells ...
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bioRxiv - Immunology 2020Quote: ... The mouse B cell isolation kit and mouse naïve CD4+ T cell isolation kit were from STEMCELL technologies. The purified rat anti-mouse α4β7 (DATK32 ...
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bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... 6 ng/mL recombinant mouse IL3 and 10 ng/mL recombinant human IL-6 (all from Stem Cell Technologies). On Day 0 ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: Total CD4+ T cells were purified with EasySep Mouse T Cell Isolation Kit (Stemcell Technology). Total splenocytes suspended in MACS buffer (2% FBS in DPBS ...
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bioRxiv - Immunology 2023Quote: ... cells were pre-isolated with EasyStep Mouse CD4+ T cell isolation Kit (STEMCELL Technologies Inc) and stained with the fluorochrome-conjugated mAbs targeting CD4 ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Immunology 2021Quote: ... EasySep Mouse Naive CD4+ T cell and CD8+ T cell Isolation Kit (StemCell Technologies, Vancouer, Canada) were purchased.
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bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
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bioRxiv - Microbiology 2020Quote: CD4+ T cells were isolated using negative selection (Stem Cell Technologies CD4 enrichment kit) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were obtained using a negative CD4 magnetic selection kit (Stemcell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated using the EasySep Human CD4 Positive Selection Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD4 cells were isolated using the Easy-Sep CD4+ T cell isolation kit (Stemcell Technologies), plated on a tissue culture-treated plate pre-coated with neutravidin (Thermo) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated using the CD4+ Negative Selection Kit (StemCell Technologies, Cat# 19752) followed by fluorescence-activated cell sorting ...
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bioRxiv - Immunology 2021Quote: Female C57BL/6 mice received a single dose of mouse recombinant CSF-3 (StemCell Technologies) at 0.25mg/kg in sterile PBS ...
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bioRxiv - Microbiology 2020Quote: ... monocytes and others (CD4− CD8− CD19− cells) using CD4 negative selection kit (STEMCELL Technologies, https://www.stemcell.com), CD8 positive selection kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... bulk CD4+ T cells were isolated using the EasySep CD4+ T cell Enrichment Kit (StemCell Technologies). Isolated CD4+ T cells were maintained in RPMI medium supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin (R10 medium) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated with EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies, 17952).
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bioRxiv - Cancer Biology 2020Quote: Both CD4+ and CD8+ cells were isolated from normal mouse splenocytes by immune-magnetic negative selection kit (Stemcell Technology, Vancouver ...
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bioRxiv - Immunology 2019Quote: OT2 T cells were isolated by magnetic beads (EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit, StemCell Technologies) from a single cell suspension of splenocytes ...
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bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
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bioRxiv - Cancer Biology 2024Quote: ... in EpiCult™-B (mouse) medium supplemented with human recombinant EGF (hrEGF) (Stem Cell Technologies, 78006), human recombinant bFGF (hrbFGF ...
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bioRxiv - Molecular Biology 2019Quote: ... CD4+ T-cells were isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2020Quote: Activation of CD4+ T-cell Targets: CD4+ T-cells were enriched by negative selection (Easysep, Stemcell Technologies), typically starting from 200×106 PBMCs per study participant ...