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Citations for Stemcell Technologies :
1 - 50 of 52 citations for Rat Cytokine Microarray since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... supplemented with CC100 cytokine cocktail (Stem Cell Technologies), 100ng/ml TPO (Peprotech ...
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bioRxiv - Cell Biology 2023Quote: ... All cytokines were purchased from Stem Cell Technologies. After 2 weeks colonies were stained with the following antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 100X StemSpan Cytokine Cocktail (STEMCELL Technologies), in the presence or absence of 100 pg/ml rh-IL21 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 100X Stem Span Cytokine Cocktail (STEMCELL Technologies), in the presence or absence of 100 pg/ml recombinant human (rh)-IL21 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 5% CO2 (MegaCult®-C Medium without Cytokines, STEMCELL Technologies). Cultures were supplemented with human recombinant 50 ng/ml TPO (Peprotech) ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with StemSpan CC100 cytokine mix (Stem Cell Technologies Inc.) and 2% Penicillin-Streptomycin for a total of 6 days.
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bioRxiv - Cancer Biology 2022Quote: ... Cytokines were either purchased from STEMCELL Technologies (Vancouver, BC, Canada) or BioLegend (San Diego ...
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bioRxiv - Molecular Biology 2020Quote: ... and human cytokine cocktail (StemSpan™ CC100, Stemcell Technologies, Cat.No. 02690). The seeded cells were then treated with ...
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bioRxiv - Molecular Biology 2020Quote: ... and human cytokine cocktail (StemSpan™ CC100, Stemcell Technologies, Cat.No. 02690). The seeded cells were treated with MEISi-1 and MEISi-2 small molecules at 0,1 µM ...
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bioRxiv - Bioengineering 2021Quote: ... additionally supplemented with cytokines (Human Recombinant IL-2; Stemcell Technologies - #78036). To stimulate the cells ...
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bioRxiv - Immunology 2021Quote: ... and rat serum (Stemcell Technologies), followed by the anti-phosphoSTAT3 (Tyr705 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were kept (18h) in StemSpanTM CC100 cytokines-supplemented SFMII serum-free medium (StemCell Technologies). Human BM MSCs (hMSCs ...
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bioRxiv - Genetics 2019Quote: ... The electroporated cells were resuspended with Stemspan II media with CC100 cytokine cocktail (Stem Cell Technologies). Two guides targeting GFI1B coding regions (sgRNA1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 cells were plated in triplicate in cytokine-free methylcellulose media (Methocult GM M3234, StemCell Technologies) supplemented with 20 ng/mL IL-3 and 20 ng/mL SCF (R&D Systems) ...
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bioRxiv - Cancer Biology 2020Quote: Flow sorted cells were seeded in serum-free methylcellulose-based medium with cytokines (MethoCult SFH4436, Stemcell technologies). 5 μM imiquimod or DMSO were added to the methocult at the time of seeding in a separate location within the media prior to vortexing ...
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bioRxiv - Genomics 2021Quote: Colony-forming units (CFU) were generated in a cytokine-containing methylcellulose medium (MethoCult Media H4434, StemCell Technologies) by seeding 500 cells per well with 0.6 ml medium in a 12-well plate ...
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bioRxiv - Immunology 2021Quote: ... and/or Normal Rat Serum (STEMCELL Technologies: 13551). Flow cytometry was performed on LSR Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and/or rat serum (2%; Stem Cell Technologies) for 5min on ice prior to adding fluorescent antibodies (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of 2% rat serum (Stemcell technologies) was added to each well for 15 minutes at room temperature and proceeded to further staining without a washing step.
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bioRxiv - Cell Biology 2020Quote: ... Akt-transduced BMECs were selected for seven days in serum- and cytokine-free StemSpan SFEM (StemCell Technologies, Inc. 09650) media ...
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bioRxiv - Biophysics 2020Quote: ... The cells were resuspended in 1 mL of medium contained 1x Stem Span Cytokine Cocktail CC100 (Stem Cell Technologies, Canada) in 24-well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with NeuroCult proliferation kit (mouse & rat, STEMCELL Technologies, #05702), human recombinant EGF (20 ng/mL ...
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bioRxiv - Systems Biology 2024Quote: ... Mouse and Rat proliferation supplement 10% (Stem Cell Technologies #05701), Recombinant Human FGF (20 ng/mL ...
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells where count and seeded at a density of 2×10^4 cells per replicate into cytokine-supplemented methylcellulose medium (MethoCult M3434, Stemcell Technologies). After 10-14 days the colonies propagated and scored ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed in IMDM+20%FCS and plated into 1.4 mL of cytokine-rich methylcellulose (Methocult H4435, Stem Cell Technologies). Colonies were scored 14 days later under an inverted microscope ...
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bioRxiv - Genetics 2020Quote: BM MNCs cells were seeded on 35-mm culture dishes in triplicate in methylcellulose medium supplemented with cytokines (MethoCult GF M3434, STEMCELL Technologies). CFU-Cs (colony forming units in culture ...
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bioRxiv - Immunology 2019Quote: Cord blood CD34+ hematopoietic stem cells were purchased and cultured in StemSpan II media with the MK supplemental cytokines (StemSpan II, Stemcell Technologies, Inc.), according to the culture and differentiation protocols from Stemcell Technologies (Stemcell Technologies Inc ...
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bioRxiv - Cell Biology 2021Quote: ... 100ug were added to 50K GMPs sorted the day before and cultured in StemSpan SFEMII supplemented with 1% L-Glutamine and Penicillin/Streptomycin with no cytokines (Stem cell technologies). Cells were cultured in a humidified incubator at 37°C and 5% CO2 for 12 hours and then washed twice with PBS-2%FBS with 7AAD ...
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bioRxiv - Cancer Biology 2022Quote: 1×105 sorted CD34+ HSPCs were seeded in for collagen-based colony-forming assays (MegaCult™-C medium with cytokines, StemCell Technologies), as previously described (Gialesaki et al. ...
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bioRxiv - Bioengineering 2021Quote: 5 ×10 3 Floating cells collected at different time points (day 8, day 10, and day 12) were grown in 1 mL of cytokine containing MethoCult H4434 medium (StemCell Technologies, Vancouver) at 37° C ...
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bioRxiv - Developmental Biology 2021Quote: Cells were plated at 2 days after cell sorting (Day 6 post differentiation) into a methylcellulose-based medium containing hematopoietic cytokines (MethoCult M3434, StemCell Technologies, Cat. 03434) at 6000 cells/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... We then collected the cells and plated them at the same concentration (using RPMI) into cytokine enriched methylcellulose media for colony formation (MethoCultTMGF H4434, StemCell Technologies, Vancouver, CA). After 7 days at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Erythroblasts were cultured in a serum-free mononuclear cell (MNC) medium containing the following cytokines diluted in Stem Span Serum Free Expansion Medium (Stem Cell Technologies, CA): 40□ng/mL insulin-like growth factor 1 (IGF1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cryopreserved MDS cells were thawed and cultured in StemSpan™ + cytokines (10ng/ml hIL3, 10ng/ml hSCF, 5ng/ml hIL6) overnight before plating cells in Methocult H4034 Optimum (Stem Cell Technologies) at a density of 1-2 × 104 cells per mL with DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... assay done by culturing 100-sorted cells LIN-CD34+ CD38- CD9+ or CD9- in to MethoCult™ H4434 with human cytokine (Stem Cell Technologies) and colony formation score at day 12-14 of incubation in 37°C.
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bioRxiv - Cancer Biology 2023Quote: HSCs from the BM of CD99 Gt/Gt and littermate WT mice were double sorted to >95% purity and seeded at a density of 150 cells/replicate into cytokine-supplemented methylcellulose medium (Methocult M3434; Stem Cell Technologies, Vancouver, Canada). Colonies number was scored after 10 days ...
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bioRxiv - Cell Biology 2023Quote: Transduced lineage negative (Lin-) or Lin-Sca-1+Kit+ (LSK) cells were plated into 1mL of methylcellulose-based medium with cytokines (Stem Cell Technology, MethoCult GF M3434) into 35 x 10 mm non-adherent Bio-One Petri Dish ...
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bioRxiv - Genetics 2021Quote: Add normal rat serum from the EasySep Mouse T-cell Isolation Kit (StemCell Technologies) and Isolation Cocktail ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with normal rat serum (50 µl/ 1×108 via.cells, Stem Cell Technologies and Jackson ImmunoResearch Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... primary rat hepatocytes were plated in AggreWell Micromolds (400μm square AggreWell micromolds, Stem Cell Technologies) along with NHDFs at a 1:1.6 ratio and incubated in a hepatocyte medium containing DMEM with L ...
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bioRxiv - Cancer Biology 2022Quote: ... was cultured in standard mouse NSC media containing NeuroCult basal medium (mouse & rat, STEMCELL Technologies, #05700) supplemented with NeuroCult proliferation kit (mouse & rat ...
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bioRxiv - Neuroscience 2021Quote: ... from embryonic day 18 (E18) rats were dissected and resuspended in BrainphysTM medium (BPNM, Stemcell Technologies; 05790) supplemented with 2% SM1 (Stemcell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... E18 rat hippocampal tissue (Brain Bits) were dissociated with papain (Brain Bits) and DNase I (Stemcell Technologies) following the manufacturer instructions for neuron dissociation ...
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bioRxiv - Neuroscience 2023Quote: ... cells were cultured into 96-well low-attachment plates containing NeuroCult Basal Media (Mouse and Rat; STEMCELL Technologies) with Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... hippocampi were dissected from embryonic day 18 (E18) rats and resuspended in BrainPhys medium (BPNM, STEMCELL Technologies, 05790) supplemented with 2% SM1 (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... DF-AA27 GEMM GSCs were cultured in NeuroCult Basal Medium (Mouse and Rat) with Proliferation Supplement (StemCell Technologies 05702) supplemented with EGF (20 ng/mL) ...
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bioRxiv - Immunology 2019Quote: ... Cells were then permeabilized with 0.3% Triton-X for 15 min followed by 30 min block in 0.1% Triton-X + 5% normal rat serum (Stem Cell Technologies). Cells were incubated in Drp1 p616 Ab overnight at 4°C in 0.1% Triton-X + 1% BSA and then in secondary antibodies (AF488 Goat anti-Rabbit) ...
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bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...