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Citations for Stemcell Technologies :
1 - 50 of 1280 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Monocytes were enriched from the same leukapheresis products using RosetteSep kits (Stemcell Technologies), and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech ...
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bioRxiv - Immunology 2019Quote: Monocytes were enriched from the same leukopoiesis products as T-cells using RosetteSep kits (Stemcell Technologies), and were then cultured at 1-2×106/ml in 12 well-plates with differentiation media containing 50 ng/ml Interleukin 4 (IL-4 ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... a second bead-based purification step was included according to the manufacturer’s instructions (EasySepTM Human Isolation Kit from STEMCell Technologies, Cologne, Germany). While density centrifugation led to 90% pure PMN ...
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bioRxiv - Genomics 2020Quote: ... Neutrophils were freshly isolated directly from whole blood by negative selection immunomagnetic purification with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies; cat. no. 19666).
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bioRxiv - Immunology 2023Quote: ... Intermediate layer containing IELs was collected and washed with 1X PBS followed by either flowcytometry analysis or used for CD8α IELs purification using EasySep™ mouse CD8α positive selection kit-II (StemCell Technologies; Cat# 18953). Sort purified CD8α IELs (50,000/mouse ...
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bioRxiv - Immunology 2024Quote: Total CD4+ T-cells were obtained by negative selection from the spleen of wild-type mice using EasySepTM magnetic separation per kit instructions (product number 19851, STEMCELL Technologies, Vancouver, Canada). Purified total CD4+ were enumerated and 5×105 total CD4+ T-cells were injected via tail vein to recipient mice ...
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bioRxiv - Microbiology 2022Quote: ... Cell purification used negative selection employing the Rosette-Sep platform (Stemcell Technologies) and were carried out by the Penn CFAR Immunology Core ...
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bioRxiv - Cancer Biology 2021Quote: ... Further purification was done using a iodixanol (#07820, Stem Cell Technologies, Vancouver, Canada) gradient ...
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bioRxiv - Bioengineering 2021Quote: Leukapheresis products from anonymous healthy human donors were purchased from STEMCELL Technologies, Inc ...
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bioRxiv - Immunology 2023Quote: Leukapheresis product (leukopaks) from healthy human donors were obtained from Stemcell Technologies shipped on wet ice ...
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bioRxiv - Microbiology 2023Quote: Human blood products from healthy donors were purchased from StemCell Technologies (Vancouver, Canada). Peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Immunology 2020Quote: ... CD4+ or CD3+ cells were enriched from leukopheresis products through negative selection (RosetteSep, STEMCELL Technologies) before density gradient centrifugation ...
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bioRxiv - Cancer Biology 2024Quote: ... Common Rule (Category 4) or 2) de-identified donor leukapheresis products obtained from StemCell Technologies. CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Common Rule (Category 4) or 2) de-identified donor leukapheresis products obtained from StemCell Technologies. CD3+ T-cell populations were isolated using EasySep Human T Cell Isolation Kit (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Control T cells were isolated from from leukapheresis products from healthy donors (STEMCELL Technologies, Vancouver, BC, Canada). This study was approved by the Research Ethics Board of the BC Cancer Agency/University of British Columbia (certificates H16-00291-A021 ...
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bioRxiv - Immunology 2023Quote: ... BMDCs were further enriched by isolating loosely adherent cells removing F4/80+ cells then isolating CD11c+ cells by bead purification following manufacturer’s instructions (Stem Cell Tech). Cells were then plated in tissue culture treated dishes in DMEM with 10% FBS then used the following day for experiments.
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bioRxiv - Immunology 2021Quote: PBMCs from three independent donors were isolated from leukapheresis products using Lymphoprep density gradient centrifugation and SepMate PBMC isolation tubes (StemCell Technologies), under the Weatherall Institute of Molecular Medicine ...
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bioRxiv - Cell Biology 2023Quote: CD34+ cells from the infusion product or purified from bone marrow mononuclear cells post-transplantation were plated in MethoCult H4435 Enriched media (STEMCELL Technologies, Vancouver, Canada) in 35-mm dishes at a concentration of 1000 cells/ml and incubated at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2020Quote: ... We used the commercial kit (STEMdiff™ Cerebral Organoid Kit, StemCell Technologies 08570) with previously described modifications to increase embryoid body surface area75 ...
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bioRxiv - Cancer Biology 2020Quote: ... The ALDEFLUOR kit (Stemcell Technologies) was used to quantify the ALDH enzymatic activity ...
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bioRxiv - Neuroscience 2023Quote: ... StemDiff Hematopoietic Kit (Stemcell Technologies) was used to generated hematopoietic precursor cells (HPCs) ...
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bioRxiv - Immunology 2023Quote: ... Aldefluor kit (01700, Stemcell Technologies) was used for assessing RALDH activity ...
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bioRxiv - Genomics 2023Quote: ... CD14+ monocytes were purified using EasySep Human Monocytes Isolation Kit (negative selection kit; Stem cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ALDEFLUOR kit (StemCell technologies Cat #01700) was used for identification of cancer stem cells that express high levels of ALDH ...
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bioRxiv - Cancer Biology 2020Quote: The Aldefluor kit (Stem Cell Technologies) was used to characterize the ALDH activity of 4T1 cells as described in (Pearce et al ...
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bioRxiv - Molecular Biology 2021Quote: ... All kits are from STEMCELL Technologies, and differentiation was induced following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... The Aldefluor kit (Stem Cell Technologies) was used for the staining and quantification of ALDH1 activity by flow cytometry ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... The ALDEFLUOR kit (StemCell Technologies, USA) was used to assess the population with a high ALDH enzymatic activity ...
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bioRxiv - Cancer Biology 2020Quote: ALDEFLUOR kit (STEMCELL Technologies, Cologne, Germany) was used to identify cells expressing high levels of ALDH enzyme ...
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bioRxiv - Neuroscience 2021Quote: ... STEMdiff Trilineage Differentiation Kit (StemCell Technologies) was used as described by the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... Neurocult Enzymatic Dissociation kit (Stemcell Technologies) was used for cell dissociation according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Selection kit II (STEMCELL Technologies, #17877) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: StemDiff T cell kit (StemCell Technologies) was used to differentiate HSPCs to lymphoid progenitor cells and to DP T cells ...
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bioRxiv - Neuroscience 2023Quote: ... and frozen at −80°C for qRT-PCR or Neurosphere Basal Medium supplemented with Proliferation Supplement (STEMCELL Technologies) as described below ...
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bioRxiv - Immunology 2020Quote: ... The mouse B cell isolation kit and mouse naïve CD4+ T cell isolation kit were from STEMCELL technologies. The purified rat anti-mouse α4β7 (DATK32 ...
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bioRxiv - Immunology 2020Quote: ... EasySep human CD4+ T cell enrichment kit and EasySep human CD8+ T cell enrichment kit (STEMCELL Technologies, Canada), respectively ...
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bioRxiv - Immunology 2020Quote: ... the EasySep Kit (StemCell Technologies, cat. 18000) was used according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... NK-cell Enrichment Kit (Stem Cell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the ALDEFLUOR kit was used (StemCell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... using memory CD8+ isolation kit (StemCell Technologies). T cells were harvested and washed three times with Opti-MEM (LifeTechnologies) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... STEMdiff Cerebral Organoid Kit (StemCell Technologies, 08570) was used for organoid culture ...
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bioRxiv - Immunology 2022Quote: ... and then the ALDEFLUOR kit (Stemcell Technologies) protocol was followed ...
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bioRxiv - Developmental Biology 2023Quote: ... The hPSC Genetic Analysis Kit (Stemcell Technologies) was used following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... StemLight Pluripotency Antibody Kit (Stem Cell Technologies) was used for pluripotency testing ...
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bioRxiv - Cell Biology 2024Quote: ... and c-Kit/CD117 antibodies (STEMCELL Technologies) for 30 min ...
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bioRxiv - Cell Biology 2019Quote: Whole-brain cerebral organoids were generated with a commercial kit (STEMdiff Cerebral Organoid Kit, STEMCELL Technologies, Catalogue Number 08570), which has been adopted and optimized based on previously published protocol50 ...
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bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...