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Citations for Stemcell Technologies :
1 - 50 of 994 citations for Lymphocyte antigen 6H LY6H Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies; cat ...
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bioRxiv - Immunology 2020Quote: ... Isolated CD3+ cells and tumor-infiltrating lymphocytes (TILs) were maintained in Human T Cells Expansion (STEMCELL Technologies, Vancouver, Canada) medium supplemented with 5% of heat-inactivated human AB serum (Omega Scientific ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 6h in hypoxia incubator chamber (Stem cell Technologies, #27310) at 37°C and 1% CO2.
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bioRxiv - Immunology 2021Quote: ... 100μL of Isolation Cocktail and 100μL of Rapid Spheres (Easy Sep™ Direct Human Total Lymphocytes Isolation Kit: 19655, StemCell Technologies) were added to the remaining whole blood ...
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bioRxiv - Immunology 2021Quote: Primary NK cells were isolated from peripheral blood lymphocytes (PBLs) of healthy donors using the EasySep™ human NK Cell enrichment kit (STEMCELL Technologies). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... Human CD4+ T cells were isolated from approximately 2 x 108 elutriated lymphocytes to approximately 90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (15062; Stem Cell Technologies), using Ficoll/Hypaque (45-001-749 ...
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bioRxiv - Immunology 2023Quote: ... The resulting cell suspensions were enriched for lymphocytes using Lymphoprep (Stemcell Technologies). Following enrichment ...
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bioRxiv - Immunology 2020Quote: ... T lymphocytes were purified by negative-selection using Rosette tetramers (Stemcell Technologies Inc) and the purity was monitored by flow cytometry ...
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bioRxiv - Microbiology 2019Quote: ... freshly isolated PBMCs obtained from healthy donor peripheral blood (lymphocyte separation media; StemCell Technologies) were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend) ...
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bioRxiv - Microbiology 2019Quote: ... freshly isolated PBMCs obtained from healthy donor peripheral blood (lymphocyte separation media; StemCell Technologies) were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend) ...
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bioRxiv - Microbiology 2019Quote: Freshly isolated PBMCs obtained from healthy donor peripheral blood (lymphocyte separation media; StemCell Technologies) were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... Mononuclear cells were recovered with lymphocyte separation media and sepMate tubes (Stemcell Technology, Vancouver, Canada) by centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... and T lymphocytes purified using Easy SepTM Mouse T cell isolation kit (STEMCELL Technologies, Cat# 19851A), as we described83 ...
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bioRxiv - Immunology 2022Quote: ... Naïve splenic B lymphocytes were purified by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies). Primary B cells from each transgenic mouse line were cultured in RPMI 1640 with L-glutamine supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T lymphocytes were negatively selected for using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies #19851) and cultured in RPMI containing L-glutamine (Corning # MT10040CM ...
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bioRxiv - Microbiology 2021Quote: ... and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads according to the manufacturer’s instructions (StemCell Technologies). The cells were activated with phytohemagglutinin-L (10 μg/mL ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from elutriated lymphocytes by negative selection using RosetteSep CD4+ T cell enrichment cocktail (StemCell Technologies) followed by Ficoll/Hypaque (Amersham Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... splenic T lymphocytes were isolated from Cas9 transgenic mice using negative selection with the EasySep Mouse T Cell Isolation Kit (Stemcell Technologies) and then activated with Dynabeads Mouse T-Activator CD3/CD28 beads (Cat # 11456D ...
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bioRxiv - Microbiology 2021Quote: ... and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies, Vancouver, BC) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T lymphocytes were then purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies, Vancouver, BC). CD4+ T lymphocytes were activated with phytohemagglutinin-L (10 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... PBMC were isolated from blood collected in ethylenediaminetetraacetic acid-anticoagulated (EDTA) tubes by lymphocyte separation medium density gradients (Stemcell technologies, cat# 07801) and resuspended in PRMI 1640 medium supplemented with 10% FCS ...
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bioRxiv - Immunology 2024Quote: ... were cultured with lymphocytes (2.5 × 104) magnetically sorted frommousespleen singlecell preparations using MACs columns and the EasySep™ Mouse TCell Isolation Kit (StemCell Technologies Cat# 19851) for a total of 6d with 20 units/mL of IL-2 ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs were obtained by leukapheresis and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the instructions of the manufacturer (StemCell Technologies, Vancouver, BC, Canada) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were obtained by leukapheresis and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the instructions of the manufacturer (StemCell Technologies, Vancouver, BC, Canada) and were activated with phytohemagglutinin-L (10 μg/ml ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell pellet was resuspended in 500 µL HBBS with 0.5% fatty acid-free BSA and lymphocytes were isolated by using the EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies, Vancouver, British Columbia, Canada) with the protocol provided ...
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bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Microbiology 2019Quote: Harvested human blood was incubated with RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies) or RosetteSep Human B cell enrichment cocktail (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... Human PBMCs were activated with ImmunocultTM CD3/CD28 Human T cell Activators (STEMCELL Technologies, anti-human CD3 and anti-human CD28 monospecific antibody complexes ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... human PBMC (ST70025, STEMCELL Technologies) was mixed and incubated with MACS CD4 MicroBeads (130-045-101 ...
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bioRxiv - Cell Biology 2022Quote: ... IntestiCult™ Organoid Growth Medium (Human) or IntestiCult™ Organoid Differentiation Medium (Human) were used for culture or differentiation of human gut organoids (STEMCELL Technologies, Vancouver, Canada). Organoids were used prior to passage 3 for optimal efficiency of EEC and β-like cell induction.
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bioRxiv - Immunology 2020Quote: Primary human NK cells were enriched from the peripheral blood of human donors using RosetteSep Human NK cell Enrichment Cocktail (Stem Cell Technologies, Cat #15065) and cultured overnight in RPMI-1640 (Corning ...
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bioRxiv - Cancer Biology 2022Quote: ... human leukemia cells were isolated using EasySepTM Mouse/Human Chimera Isolation Kit (#19849; Stemcell Technologies). All animal experiments were approved by and performed in accordance with guidelines of the Institutional Animal Care and Use Committee at University of Colorado (protocol No ...
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bioRxiv - Immunology 2021Quote: ... human neutrophils were purified from blood using the EasySep™ Human Neutrophil Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
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bioRxiv - Cell Biology 2020Quote: Human Monocyte were acquired from frozen PBMCs with STEMCELL EasySep Human Monocyte Enrichment kit (STEMCELL TECHNOLOGIES). Purified monocytes were cultured in RPMI-1640 Glutamax medium (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Human pDC isolation was performed using an EasySep™ Human Plasmacytoid DC Enrichment kit (STEMCELL Technologies) following the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... 10μl Human LDL (StemCell Technologies, 02698) for every 50ml media and the following cytokines (all from MiltenyiBiotec unless stated otherwise) ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.02% Human LDL (Stem Cell Technologies) and the following cytokines (Miltenyi Biotec unless stated otherwise) ...
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bioRxiv - Immunology 2020Quote: ... commercial cryo-human pDC (Stemcell Technology) from 5 healthy subjects ...
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bioRxiv - Bioengineering 2023Quote: ... Human recombinant VEGF-165 (Stemcell technologies; Saint Egrève ...
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bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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bioRxiv - Immunology 2022Quote: B cells were enriched from human PBMCs using EasySep Human Pan-B Cell Enrichment Kit (StemCell Technologies) before being incubated with Fc block (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...