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Citations for Stemcell Technologies :
1 - 50 of 1012 citations for IL 2R alpha CD25 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD25-PE-Cy7 (clone MA251) plus FVD-eFluor-780 (eBioscience) and human FcR blocking Reagent (StemCell Technologies). Cells were washed then fixed and permeabilized using the eBioscience FOXP3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Neuroscience 2021Quote: ... TNF alpha (0.5ng/mL final concentration in PBS, human recombinant, Stemcell Tech) was added to half of the wells ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12 (Stemcell Technologies, Vancouver, Canada), IL-15 (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Molecular Biology 2021Quote: ... and human recombinant IL-2 (Stemcell Technologies, Vancouver, Canada). Activated CD4+ T cells were transfected with pVax-CasRx and gRNA plasmids by nucleofection using Lonza 4d nucleofector ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml human recombinant IL-6 (STEMCELL Technologies) and 10 ng/ml mouse recombinant IL-3 (R&D ...
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bioRxiv - Bioengineering 2021Quote: ... additionally supplemented with cytokines (Human Recombinant IL-2; Stemcell Technologies - #78036). To stimulate the cells ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 ng μL-1 recombinant human IL-2 (StemCell Technologies) and activated with the ImmunoCult Human CD3/CD28 T Cell Activator (StemCell Technologies) ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/mL recombinant human IL-15 (StemCell Technologies, Cat # 78031). Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... 1000 cells of each sorted population were seeded in 1 mL of methylcellulose medium supplemented with recombinant murine SCF and IL-3 with recombinant human IL-6 and Epo (M3434, STEMCELL Technologies) in duplicate ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with Human Recombinant IL-2 (12.5 ng/mL) (Stemcell Technologies, Cat.#78036), with initial seeding at 1×106 alive cells/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with Human Recombinant IL-2 (12.5 ng/mL) (Stemcell Technologies, Cat.#78036), IL-7 (5 ng/mL ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10 ng/mL of Human Recombinant IL-2 (STEMCELL TECHNOLOGIES, #78220) and ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL TECHNOLOGIES ...
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bioRxiv - Neuroscience 2023Quote: ... CD4+ cells were subjected to CD25+ cell isolation (STEMCELL Technologies). Cells were resuspended at 106 cells per 200μl saline and injected i.p ...
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bioRxiv - Biophysics 2023Quote: ... with the addition of Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Biophysics 2023Quote: ... with the addition of ImmunoCult Human CD3/CD28/CD2 T Cell Activator and Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA) as per STEMCELL Technologies Inc ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of ImmunoCult Human CD3/CD28/CD2 T Cell Activator and Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA) as per STEMCELL Technologies Inc ...
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bioRxiv - Immunology 2021Quote: ... from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (IL-15) (STEMCELL Technologies). NK cells were incubated with immune complexes ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Immunology 2021Quote: Cells were sequentially enriched for CD4+ and CD25+ cells by RosetteSep (STEMCELL Technologies) and CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2023Quote: ... magnetically isolated rCD4 (CD3+/CD4+/CD69-/ CD25-/ HLA-DR-) T cells (Stemcell Technologies) were rested in the presence of antiretroviral drugs (10nM Raltegravir and 20nM Efavirenz) ...
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bioRxiv - Cell Biology 2024Quote: ... An antibody to CD25-PE (Clone 2A3, #60153PE) was obtained from StemCell Technologies. Antibodies to SOX2 (#MAB2018) ...
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bioRxiv - Cell Biology 2024Quote: ... interleukin (IL) 6 and IL 11 (StemCell Technologies). Cultures were maintained at 37°C in a 5% CO2 humidified atmosphere for 14 days with media changes on days 5 ...
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bioRxiv - Neuroscience 2023Quote: ... Isolated CD4+ T cells were then subjected to a CD25+ cell isolation (STEMCELl Technologies) using releasable magnetic spheres (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Alpha MEM with nucleosides (StemCell Technologies, Vancouver, Canada) were purchased for cell culture purposes ...
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bioRxiv - Molecular Biology 2019Quote: ... 6 ng/mL recombinant mouse IL3 and 10 ng/mL recombinant human IL-6 (all from Stem Cell Technologies). On Day 0 ...
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bioRxiv - Microbiology 2023Quote: In vitro IL-10 assays were also performed using human Peripheral Blood Mononuclear Cells (PBMCs) (STEMCELL Technologies, Cat. # 70025.1). The assay was performed as described above ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Physiology 2021Quote: ... mice fed a normal chow diet were injected with vehicle (saline or PBS), 300U/kg or 3000U/kg of recombinant human erythropoietin (EPREX, Janssen-Cilag) or 50ng/g IL-1β (STEMCELL Technologies). All animal use complied with the guidelines of the Canadian Committee for Animal Protection and was approved by IRCM Animal Care Committee.
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Synthetic Biology 2021Quote: ... 20 mM cyclic Pifithrin-Alpha (Stem Cell Technologies cat# 72062) and 20 ng/ml human recombinant bFGF (Stem Cell Technologies cat# 78003) ...
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bioRxiv - Molecular Biology 2023Quote: ... TNFα treatment (25 ng/ml rh TNF-alpha, STEMCELL Technologies) was performed in DMEM without FCS for 20 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Stem Cell Technologies). Cytological analysis of blood and bone marrow smears from Inpp4b+/+ and Inpp4b-/- leukemic mice was done by classical wright-giemsa staining (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... + 10 ng/ml IL-15 and 10 IU of IL-2 (Stemcell Technologies, Canada). The culture media was replenished every three days with fresh media containing 10 IU of IL-2 and 10 ng/ml IL-15 ...
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bioRxiv - Immunology 2022Quote: ... + 10 ng/ml IL-15 and 10 IU of IL-2 (Stemcell Technologies, Canada). The culture media was replenished every three days with fresh media containing 10 IU of IL-2 and 10 ng/ml IL-15 ...
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bioRxiv - Bioengineering 2023Quote: ... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
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bioRxiv - Immunology 2023Quote: ... one piece (∼3g) was used for CD25+CD8- Treg enrichment using a custom thymic Treg selection kit (STEMCELL Technologies)102 ...
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Cancer Biology 2023Quote: ... + 10ng/ml IL-3 (STEMCELL Technologies). The WSU-AML cell line was also seeded at 1x106 cells/ml ...
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bioRxiv - Neuroscience 2023Quote: ... we immunomagnetically isolated natural Treg from wild type female mice or Foxp3-RFP male and female mice using a CD4+ and CD25+ Treg isolation kit (STEMCELL Technologies) as described below ...
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bioRxiv - Cell Biology 2021Quote: ... 100μg/mL IL-6 (StemCell Technologies 78050), 35nM UM171 (StemCell Technologies 09655 ...
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bioRxiv - Molecular Biology 2022Quote: ... & 300 IU/mL IL-2 (STEMCELL Technologies). T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... IL-9 and TPO (02696, STEMCELL Technologies, Canada) for induction of megakaryopoiesis ...
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bioRxiv - Cancer Biology 2019Quote: ... IL-3/G-CSF–producing M2-10B4 and IL-3/KL-producing SI/SI murine fibroblasts in MyeloCult H5100 (StemCell Technologies) supplemented with hydrocortisone ...
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bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...