Labshake search
Citations for Stemcell Technologies :
1 - 50 of 1548 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... HIV-negative donors using the RosetteSep Human CD4 T cell enrichment kit (StemCell Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
-
bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
-
bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
-
bioRxiv - Microbiology 2023Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
-
bioRxiv - Microbiology 2023Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
-
bioRxiv - Molecular Biology 2022Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using Lymphoprep™ and SepMate™ tubes (StemCell Technologies), and cryopreserved [41] ...
-
bioRxiv - Microbiology 2021Quote: ... 1 × 105 human NK cells isolated from PBMC using EasySep human NK cell isolation kit (Stem Cell Technologies) were added to wells in the presence of CD107a PE (BD ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Leukemia Inhibitory Factor (LIF, STEMCELL Technologies, 78055.1) and 10 ng/mL human recombinant Epidermal Growth Factor (EGF ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Leukemia Inhibitory Factor (LIF, STEMCELL Technologies, 78055.1) and 10 ng/mL human recombinant Epidermal Growth Factor (EGF ...
-
bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 ng μL-1 recombinant human IL-2 (StemCell Technologies) and activated with the ImmunoCult Human CD3/CD28 T Cell Activator (StemCell Technologies) ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1), 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF ...
-
bioRxiv - Cancer Biology 2019Quote: ... and stained with 1:25 anti-human CD45 FITC (clone 2D1, STEMCELL Technologies), 1:50 anti-human CD45 APC-ef780 (clone HI30 ...
-
bioRxiv - Bioengineering 2023Quote: ... Human iPS cells were cultured routinely in mTeSR-1 media (StemCell Technologies, #05825) on 1% Geltrex-coated plates (Invitrogen ...
-
bioRxiv - Bioengineering 2023Quote: ... Human iPS cells were cultured routinely in mTeSR-1 media (StemCell Technologies, #05825) on 1% Geltrex-coated plates (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... To inhibit these pathways we used Recombinant Human Noggin Protein for BMP4 inhibition and/or XAV-939 for WNT inhibition (StemCell Technologies).
-
bioRxiv - Neuroscience 2022Quote: Human iPSC (XCL-1) derived neural progenitor cells (NPCs, Stem Cell Tech cat#70901) were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560 ...
-
bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
-
bioRxiv - Physiology 2019Quote: ... the colonoids were treated with a 1:1 mix of growth medium and IntestiCult-Human culture medium (StemCell Technologies). Colonoids were then dissociated into small cell aggregates (less than 40μm in size ...
-
bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... and cultured in a 1:1 ratio of DMEM-F12 and NeuroCult NS-A Basal Medium (Human) (Stem Cell Technologies) with 1X N-2 supplement (Gibco) ...
-
bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
-
bioRxiv - Immunology 2021Quote: ... cells were preactivated for 5 days with IL-2 and either 1:1 aAPCs or 25 μl/ml CD3/CD28/CD2 tetramer (ImmunoCult Human CD3/CD28/CD2 T Cell Activator, STEMCELL Technologies). L cells (ATCC CRL-2648 ...
-
bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
-
bioRxiv - Genomics 2024Quote: ... Cells were stimulated with ImmunoCult Human CD3/CD28 T Cell Activator Mix (1:80; StemCell Technology) and incubated at 37°C ...
-
bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
-
bioRxiv - Microbiology 2019Quote: Harvested human blood was incubated with RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies) or RosetteSep Human B cell enrichment cocktail (STEMCELL Technologies ...
-
bioRxiv - Immunology 2023Quote: ... Human PBMCs were activated with ImmunocultTM CD3/CD28 Human T cell Activators (STEMCELL Technologies, anti-human CD3 and anti-human CD28 monospecific antibody complexes ...
-
bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... human PBMC (ST70025, STEMCELL Technologies) was mixed and incubated with MACS CD4 MicroBeads (130-045-101 ...
-
bioRxiv - Cell Biology 2022Quote: ... IntestiCult™ Organoid Growth Medium (Human) or IntestiCult™ Organoid Differentiation Medium (Human) were used for culture or differentiation of human gut organoids (STEMCELL Technologies, Vancouver, Canada). Organoids were used prior to passage 3 for optimal efficiency of EEC and β-like cell induction.
-
bioRxiv - Immunology 2020Quote: Primary human NK cells were enriched from the peripheral blood of human donors using RosetteSep Human NK cell Enrichment Cocktail (Stem Cell Technologies, Cat #15065) and cultured overnight in RPMI-1640 (Corning ...
-
bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
-
bioRxiv - Cancer Biology 2022Quote: ... human leukemia cells were isolated using EasySepTM Mouse/Human Chimera Isolation Kit (#19849; Stemcell Technologies). All animal experiments were approved by and performed in accordance with guidelines of the Institutional Animal Care and Use Committee at University of Colorado (protocol No ...
-
bioRxiv - Immunology 2021Quote: ... human neutrophils were purified from blood using the EasySep™ Human Neutrophil Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol.
-
bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
-
bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
-
bioRxiv - Cell Biology 2020Quote: Human Monocyte were acquired from frozen PBMCs with STEMCELL EasySep Human Monocyte Enrichment kit (STEMCELL TECHNOLOGIES). Purified monocytes were cultured in RPMI-1640 Glutamax medium (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: Human pDC isolation was performed using an EasySep™ Human Plasmacytoid DC Enrichment kit (STEMCELL Technologies) following the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs; H9 line) were cultured in mTeSR™1 basal medium (Stem Cell Technologies), in 6-well plates coated with matrigel (Corning Matrigel Matrix hESC-qualified) ...
-
bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
-
bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
-
bioRxiv - Genetics 2019Quote: ... 10μl Human LDL (StemCell Technologies, 02698) for every 50ml media and the following cytokines (all from MiltenyiBiotec unless stated otherwise) ...
-
bioRxiv - Cancer Biology 2020Quote: ... 0.02% Human LDL (Stem Cell Technologies) and the following cytokines (Miltenyi Biotec unless stated otherwise) ...
-
bioRxiv - Immunology 2020Quote: ... commercial cryo-human pDC (Stemcell Technology) from 5 healthy subjects ...
-
bioRxiv - Bioengineering 2023Quote: ... Human recombinant VEGF-165 (Stemcell technologies; Saint Egrève ...
-
bioRxiv - Microbiology 2024Quote: ... Monolayer cultures were seeded into 96-well plates using a 1:1 ratio of IntestiCult™ Organoid Growth Medium Human Basal Medium (StemCell Technologies, 100-0190) and Organoid Supplement (StemCell Technologies ...
-
bioRxiv - Immunology 2022Quote: B cells were enriched from human PBMCs using EasySep Human Pan-B Cell Enrichment Kit (StemCell Technologies) before being incubated with Fc block (BD Biosciences ...
-
bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...