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Citations for Stemcell Technologies :
1 - 50 of 1843 citations for Human Differentiation Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Human enteroid differentiation was initiated by replacing expansion media with differentiation (Intesticult Component A (StemCell Technologies) mixed at a 1:1 ratio with DMEM/F12 supplemented with 15mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: Adipogenic differentiation was induced using Human MesenCult™ Adipogenic Differentiation Kit (STEMCELL Technologies #05412). According to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... IntestiCult™ Organoid Growth Medium (Human) or IntestiCult™ Organoid Differentiation Medium (Human) were used for culture or differentiation of human gut organoids (STEMCELL Technologies, Vancouver, Canada). Organoids were used prior to passage 3 for optimal efficiency of EEC and β-like cell induction.
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bioRxiv - Genomics 2022Quote: ... definitive endoderm differentiations were exclusively performed utilizing the STEMdiff Trilineage Endoderm Differentiation media (Stemcell Technologies, 05230). Single cell suspensions of mTeSR1 maintained ZIP13K2 hiPSCs were seeded into the respective culture formats according to the required cell-number as recommended by the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... both apical and basolateral media were removed and ALI Differentiation media (Stem Cell Technologies, Pneumacult ALI Basal Medium supplemented with 1X ALI Maintenance Supplement (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Mesenchymal: Adipocyte differentiation was carried out using the MesenCult Human Adipogenic Differentiation kit (STEMCELL Technologies #05412) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 1x Differentiation Supplement in Neurocult Media (#5704 Stem Cell Tech Kit). Cells were maintained in Differentiation Media for 7 days with half media changes every other day ...
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bioRxiv - Cancer Biology 2023Quote: ... 2ml of complete PneumaCult™-ALI differentiation media (STEMCELL Technologies, 05001) was then added to the basal compartment of each well to generate an air-liquid interface ...
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bioRxiv - Cell Biology 2023Quote: ... the media was replaced with STEMdiff™ Astrocyte Differentiation Medium (STEMCELL Technologies). Media change was performed daily ...
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bioRxiv - Cell Biology 2022Quote: ... initiation of the air-liquid interface was performed by removing media from the transwell insert and replacing the expansion media in the lower-chamber with ALI differentiation media (StemCell Technologies). At 48 hour intervals the transwell insert was rinsed with 500 μL PBS to prevent excessive mucous accumulation and the media was refreshed ...
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bioRxiv - Cell Biology 2022Quote: Two different culture media were tested for hIO growth and differentiation: one commercial (“SCT-ODM”: IntestiCult Organoid Differentiation Medium, StemCell Technologies) and one homemade cocktail (“CCM” ...
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bioRxiv - Microbiology 2023Quote: ... both apical and basolateral media were removed and ALI differentiation media (Stem Cell Technologies, Pneumacult ALI Basal Medium) supplemented with 1X ALI Maintenance Supplement (StemCell Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... both apical and basolateral media were removed and ALI differentiation media (Stem Cell Technologies, Pneumacult ALI Basal Medium) supplemented with 1X ALI Maintenance Supplement (StemCell Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... After 24 hours cells were washed with D-PBS before media was exchanged for astrocyte differentiation medium (STEMdifftm Astrocyte differentiation kit #100-0013, StemCell Technologies, Cambridge, UK) and a full media exchange was completed every day for 7 days ...
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bioRxiv - Cell Biology 2022Quote: ... cultures were changed to mouse adipogenic differentiation media (Stem Cell Technology, Vancouver Canada; 05507), allowed to differentiate ...
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bioRxiv - Microbiology 2021Quote: ... Cells were maintained in NeuroCult™ NS-A Differentiation Medium Human (STEMCELL Technologies) for up to 14 days.
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bioRxiv - Neuroscience 2022Quote: iPSC derived astrocytic cells (hiAC) were differentiated from NPCs by small molecule differentiation in STEMdiff™ Astrocyte Differentiation Media (Stem Cell Tech #cat100-0013) on Corning® Matrigel® coated tissue culture treated plates ...
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bioRxiv - Neuroscience 2021Quote: ... organoids were cultured in media comprised of 1:1 mixture of the aforementioned differentiation media and BrainPhys neuronal medium (STEMCELL Technologies). These organoids were used for functional patch-clamp recordings.
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bioRxiv - Immunology 2022Quote: ... The cells were cultured on an air-liquid interface (ALI) for differentiation in culture media (PneumaCult ALI media, STEMCELL Technologies, Canada) to allow the progenitor cells to differentiate into a mucociliary airway epithelium ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs were digested with Accutase and then differentiated onto PLO/laminin coated plates/coverslips with final differentiation media which is BrainPhys media (STEMCELL Technologies) supplemented with Glutamax (Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons differentiated with forebrain media were kept in differentiation media-STEMdiff forebrain neuron differentiation medium + supplements for one week and subsequently in maturation media – Brainphys Neuronal Medium + supplements (STEMCELL technologies) for the remaining days in culture ...
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bioRxiv - Molecular Biology 2023Quote: ... then ESCs were washed and cultured for 24 hours in neuron differentiation media [BrainPhys media supplemented with 1X SM1 neuronal supplement (STEMCELL Technologies), 1X N-2 supplement (Thermo) ...
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bioRxiv - Neuroscience 2022Quote: ... and then started differentiation by using neuronal media including NeuroCult NSC Basal Medium (StemCell Technologies) supplemented with 2% (v/v ...
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bioRxiv - Genetics 2022Quote: ... neuronal differentiation media was switched after 2 weeks to BrainPhys™ Neuronal Medium (Stem Cell Technologies) supplemented with the same factors to promote optimal neuronal activity and maturation.
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bioRxiv - Cancer Biology 2023Quote: MDSCs were generated from healthy human donors by differentiation of CD14+ monocytes isolated using a RosetteSep human monocyte enrichment kit (STEMCELL Technologies). In order to generate MDSCs ...
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bioRxiv - Microbiology 2023Quote: ... both apical and basolateral media were removed and ALI Differentiation media (Stem Cell Technologies, Pneumacult ALI Basal Medium supplemented with 1X ALI Maintenance Supplement (StemCell Technologies), 0.48 ug/mL Hydrocortisone solution (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... myeloid cells were gently thawed and washed in warm myeloid cell media (ImmunoCult™-SF Macrophage Differentiation Medium - using base media with only, M-CSF 50 ng/mL | STEMCELL Technologies, Cat. 10961), and plated in a 24-well low-attachment plate (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... Floating HPCs were then collected and plated at 50,000 cells/mL in STEMdiff Microglia Differentiation media (StemCell Technologies) for 24 days ...
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bioRxiv - Neuroscience 2022Quote: ... Floating HPCs were then collected and plated at 50,000 cells/mL in STEMdiff Microglia Differentiation media (StemCell Technologies) for 24 days ...
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bioRxiv - Microbiology 2023Quote: ... Differentiation media was prepared by mixing equal volumes of IntestiCult™ (INT) Organoid Basal Medium (Stem Cell Technologies) and complete media without growth factors (CMGF- ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were then treated with 20 uL/mL of Immunocult Human Treg Differentiation Supplement (StemCell Technologies) containing recombinant human TGF-B1 and all-trans retinoic acid ...
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bioRxiv - Bioengineering 2022Quote: ... PSC-CM differentiation was performed using the Cardiomyocyte Differentiation Kit (STEMCELL Technologies) according to manufacturer directions ...
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bioRxiv - Cell Biology 2024Quote: ... Directed differentiation was performed using the STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and fed with human IntestiCult Organoid Growth Media (STEMCELL technologies). 96 hours after plating ...
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bioRxiv - Genomics 2023Quote: ... The human iPSC-derived cardiomyocytes (hiPSC-CMs) were reprogrammed using the STEMdiff™ Cardiomyocyte Differentiation Kit (STEMCELL Technologies) as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Floating HPCs were collected and plated at 50,000 cells/mL in STEMdiff microglia differentiation media (StemCell Technologies, Vancouver, BC, Canada) for 24 days ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... Floating HPCs were collected and plated at 50,000 cells/mL in STEMdiff microglia differentiation media (StemCell Technologies, Vancouver, BC, Canada) for 24 days ...
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bioRxiv - Biophysics 2020Quote: ... differentiation was initiated using STEMdiff™ Cardiomyocyte (CM) differentiation and Maintenance Kit (StemCell technologies). At day 15 ...
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bioRxiv - Bioengineering 2020Quote: Cardiomyocyte differentiation was induced using the STEMdiff Cardiomyocyte Differentiation and Maintenance kit (STEMCELL Technologies) in the CTST platform ...
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bioRxiv - Cancer Biology 2022Quote: ... were directly sorted into a 96-well tissue culture plate containing 100 μL of differentiation media: StemSpan (Catalog #09650, StemCell Technologies), 1% Penicillin+Streptomycin ...
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bioRxiv - Neuroscience 2022Quote: NPCs were passaged and plated at 50,000 cells/cm2 in STEMdiff™ Forebrain Neuron Differentiation Media (Stem Cell Tech cat#08600) on Corning® Matrigel® coated tissue culture treated plates and transduced with a NEUROG2 lentivirus (GeneCopoeia cat#LPP-T7381-Lv105-A00-S ...
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bioRxiv - Neuroscience 2024Quote: ... In vitro differentiation was achieved by sequential culture of the cells in the following media: Step 1 (mTeSR (Stem Cell Tech) + 80 ng/ml BMP4 (PeProtech ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells were counted and seeded at a density of 10.4 × 103 cells/cm2 in differentiation media (Neurobasal medium, 2% B27 supplement, 1% GlutaMAX, and 10 μM of all-trans retinoic acid [STEMCELL Technologies]). Differentiation media was replaced every other day until day 9 post-seeding ...
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bioRxiv - Microbiology 2024Quote: ... After 4 days the monolayers of cells were subsequently maintained in an air-liquid environment with differentiation media (PneumaCult-ALI medium from STEMCELL Technologies) added to the lower (basolateral ...
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bioRxiv - Genetics 2021Quote: ... differentiation was induced as follows: (i) endoderm differentiation was induced with IDE1 (STEMCELL Technologies #72512) -containing medium (Borowiak et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... differentiation of hESCs cardiomyocytes was performed using the STEMdiffTM Cardiomyocyte Differentiation Kit (Stem Cell Technology), whereas analysis of cardiomyocyte induction efficiency was conducted via FACS using the cTnT+ primary antibody (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Differentiation was triggered by Wnt3a removal with IntestiCult™ Organoid Differentiation Medium (ODM, StemCell Technologies) for 5 days with medium changes every other day.
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bioRxiv - Neuroscience 2024Quote: Trilineage differentiation of the iPSCs was achieved using the STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Mediums were prepared based on kit instructions ...
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bioRxiv - Cell Biology 2023Quote: 2D cardiomyocyte differentiations were performed utilising either the STEMdiff ventricular cardiomyocyte differentiation kit (STEMCELL Technologies, 05010) or using a microcarrier differentiation protocol adapted from previous studies18,19.
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bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...