Labshake search
Citations for Stemcell Technologies :
1 - 50 of 1538 citations for Flap endonuclease 1 FEN 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... 1 × 105 human NK cells isolated from PBMC using EasySep human NK cell isolation kit (Stem Cell Technologies) were added to wells in the presence of CD107a PE (BD ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Leukemia Inhibitory Factor (LIF, STEMCELL Technologies, 78055.1) and 10 ng/mL human recombinant Epidermal Growth Factor (EGF ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Leukemia Inhibitory Factor (LIF, STEMCELL Technologies, 78055.1) and 10 ng/mL human recombinant Epidermal Growth Factor (EGF ...
-
bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 ng μL-1 recombinant human IL-2 (StemCell Technologies) and activated with the ImmunoCult Human CD3/CD28 T Cell Activator (StemCell Technologies) ...
-
bioRxiv - Physiology 2019Quote: ... the colonoids were treated with a 1:1 mix of growth medium and IntestiCult-Human culture medium (StemCell Technologies). Colonoids were then dissociated into small cell aggregates (less than 40μm in size ...
-
bioRxiv - Cancer Biology 2022Quote: ... and cultured in a 1:1 ratio of DMEM-F12 and NeuroCult NS-A Basal Medium (Human) (Stem Cell Technologies) with 1X N-2 supplement (Gibco) ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1), 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF ...
-
bioRxiv - Cancer Biology 2019Quote: ... and stained with 1:25 anti-human CD45 FITC (clone 2D1, STEMCELL Technologies), 1:50 anti-human CD45 APC-ef780 (clone HI30 ...
-
bioRxiv - Bioengineering 2023Quote: ... Human iPS cells were cultured routinely in mTeSR-1 media (StemCell Technologies, #05825) on 1% Geltrex-coated plates (Invitrogen ...
-
bioRxiv - Bioengineering 2023Quote: ... Human iPS cells were cultured routinely in mTeSR-1 media (StemCell Technologies, #05825) on 1% Geltrex-coated plates (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... cells were preactivated for 5 days with IL-2 and either 1:1 aAPCs or 25 μl/ml CD3/CD28/CD2 tetramer (ImmunoCult Human CD3/CD28/CD2 T Cell Activator, STEMCELL Technologies). L cells (ATCC CRL-2648 ...
-
bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
-
bioRxiv - Neuroscience 2022Quote: Human iPSC (XCL-1) derived neural progenitor cells (NPCs, Stem Cell Tech cat#70901) were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560 ...
-
bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
-
bioRxiv - Microbiology 2024Quote: ... Monolayer cultures were seeded into 96-well plates using a 1:1 ratio of IntestiCult™ Organoid Growth Medium Human Basal Medium (StemCell Technologies, 100-0190) and Organoid Supplement (StemCell Technologies ...
-
bioRxiv - Genomics 2024Quote: ... Cells were stimulated with ImmunoCult Human CD3/CD28 T Cell Activator Mix (1:80; StemCell Technology) and incubated at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
-
bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs; H9 line) were cultured in mTeSR™1 basal medium (Stem Cell Technologies), in 6-well plates coated with matrigel (Corning Matrigel Matrix hESC-qualified) ...
-
bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
-
bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
-
bioRxiv - Microbiology 2020Quote: Human iPSC (XCL-1)98-derived neural progenitor cells (hNPCs) were purchased commercially (StemCell Technologies, Cat #70901, Lot #17080) and maintained up to ten passages according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 37°C and 5% CO2 in normoxia and in pre-equilibrated human IVC1 supplemented with 50ng/mL Insulin Growth Factor-1 (IGF1) (78078, STEMCELL Technologies) under mineral oil for 1-4h to allow for recovery ...
-
bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte/stromal cell cultures were produced from HES3 human embryonic stem cells (hESCs, WiCell) maintained using mTeSR-1 (Stem Cell Technologies). hESCs were seeded at 2×104 cells/cm2 in Matrigel coated flasks and cultured for 4 days using mTeSR-1 and passaged with TrypLE (ThermoFisher) ...
-
bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... human MM cells were cultured at a density of 1 × 104 cells in MethoCult™ H4230 media (Stem Cell Technologies, #04230) supplemented with 15% FBS and 10% Gibco Phytohemagglutinin M form (PHA-M ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 μM StemRegenin 1 (StemCell Technologies, Inc.), 10% charcoal stripped FBS (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated, Walkersville, MD; or Stemcell Technologies, Vancouver, BC) via intrahepatic injection ...
-
bioRxiv - Neuroscience 2019Quote: ... Human splenocytes were then cultured at 1×106 cell per mL in ImmunoCult-XF T cell Expansion Medium (Stemcell Technologies, Vancouver, BC). For T cell stimulation ...
-
bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
-
bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 μM IWR-1-endo (StemCell Technologies, 72562), 5 μM SB-431542 (StemCell Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... NPCM is composed of Neurobasal:DMEM/F12 (1:1) supplemented with 1% N2-A (StemCell Technologies), 2% B27 without retinoic acid (Life Technologies) ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 1 µM DMH-1 (STEMCELL Technologies, cat# 73632)13 ...
-
bioRxiv - Genomics 2021Quote: ... diluted 1:40 in mTeSR-1 medium (StemCell Technologies). hiPSCs were passaged upon treatment with Accutase (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... Tra-1-60 (#60064, STEMCell Technologies at 1:200), stage-specific embryonic antigen-4 (SSEA-4 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 1 mg/mL DNase 1 (Stem Cell Technologies). The samples were digested for 1 hour at 37°C with constant low agitation using a thermomixer (Eppendorf) ...
-
bioRxiv - Molecular Biology 2023Quote: ... NeuroDiff medium is composed of Neurobasal:DMEM/F12 (1:1) supplemented with 1% N2-A (StemCell Technologies), 2% B27 (Life Technologies) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... for direct experimental use or generated in our laboratories from passage-1 human airway epithelial cells (hAEC; obtained from Epithelix SA) using the differentiation protocols and reagents provided by Stemcell Technologies (PneumaCultTM reagent series). In-house generated cultures were derived from three homozygous F508del-CFTR patients ...
-
bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
-
bioRxiv - Cancer Biology 2023Quote: ... with 1% PS and 1 μg/mL doxycycline (StemCell Technologies 72742). Prior to orthotopic injection of primary PDAC cell lines ...
-
bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies, Vancouver, BC, Canada).
-
bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... domes diluted 1:1 with advanced DMEM/F12 media (Stemcell Technologies, UK), with 50 μl domes per well ...