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Citations for Stemcell Technologies :
1 - 50 of 395 citations for CD16 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and 10 µg/mL anti-mouse CD16/CD32 antibody (as needed) (STEMCELL Technologies) for 1 hour on ice ...
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bioRxiv - Immunology 2021Quote: ... Unlabeled anti-CD16/32 (clone 2.4G2; 60161) was purchased from STEMCELL Technologies. Data were acquired using an LSRFortessa (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... FcγR-specific mAbs were obtained from Stem Cell technologies (CD16: clone 3G8; CD32: IV.3). Reverse sICs were generated from these receptor-specific antibodies using goat-anti-mouse IgG F(ab)2 fragments (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... CD14+ CD16- monocytes were isolated using magnetic negative selection (EasySep Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, Massachusetts) and differentiated into macrophages by culture in RPMI 1640 (Lonza ...
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bioRxiv - Immunology 2023Quote: Total monocytes were isolated by negative selection using the EasySep Human Monocyte Enrichment Kit without CD16 Depletion (StemCell Technologies) using the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Immunology 2023Quote: Monocytes were isolated by depletion of non-monocytic cells using a human monocyte enrichment kit without CD16 depletion (STEMCELL Technologies). PBMC were resuspended at 5ξ107 cells/mL and 50 μL/mL of the non-monocyte cocktail was added ...
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bioRxiv - Microbiology 2023Quote: ... Monocytes were isolated from PBMCs using EasySep Human monocyte enrichment kit without CD16 depletion (StemCell Technologies, Vancouver, British Columbia, Canada) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2019Quote: ... monocytes were isolated from thawed PBMCs using the EasySep Human Monocyte Enrichment Kit without CD16 Depletion (STEMCELL Technologies, Cat. No. 19058). The purified monocytes were seeded into a 24-well tissue culture treated plate at ~1.2 million cells/well in Advanced RPMI medium (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with a cocktail of lineage specific antibodies (CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and GlyA; Stemcell Technologies) for negative selection of Lin− cells using an EasySep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Preliminary experiments were also performed after isolation of monocytes using the EasySep human monocyte enrichment kit with CD16 depletion (StemCell Technologies) and the EasySep human monocyte isolation kit (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMCs by negative magnetic selection using the EasySep human monocyte enrichment kit without CD16 depletion (StemCell) following the manufacturer’s instructions and using a Big Easy magnet or Easy 50 magnet (StemCell Technologies, Vancouver, Canada). Enriched monocytes were generally >80% CD14-positive ...
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bioRxiv - Immunology 2023Quote: ... CD14+CD16-monocytes were isolated from peripheral blood mononuclear cells using immunomagnetic negative selection (EasySep Human Monocyte Isolation Kit, Stem Cell Technologies).
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bioRxiv - Cell Biology 2022Quote: ... Mouse organoid culture: we used cryopreserved mouse intestinal organoids (70931, STEMCELL Technologies) derived from the C57BL/6 mouse small intestine ...
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bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse CD8+ T cells were isolated from the mouse spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853A) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and mouse T cells were purified from mouse splenocytes using a negative selection EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies). Mouse splenocytes were prepared from spleens collected from 8-18 weeks old male BL6 mice purchased from Orient bio ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse neural stem cells (NSCs) were expanded in NeuroCult proliferation medium (mouse) (StemCell Technologies) supplemented with 20 ng/ml EGF ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary mouse glioma cells (CPEvIII) were cultured in NeuroCult proliferation medium (mouse) (StemCell Technologies) supplemented with 20 ng/ml EGF ...
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bioRxiv - Molecular Biology 2021Quote: ... Growth media was NeuroCult NSC Basal Medium (Mouse) containing NeuroCult Proliferation Supplement (Mouse) (StemCell Technologies), 0.2% heparin solution (Stem-Cell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... was cultured in standard mouse NSC media containing NeuroCult basal medium (mouse & rat, STEMCELL Technologies, #05700) supplemented with NeuroCult proliferation kit (mouse & rat ...
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bioRxiv - Bioengineering 2023Quote: The Mouse IntestiCult medium (STEMCELL Technologies) was prepared and supplemented with 100 U/mL Pen/Strep (Gibco) ...
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bioRxiv - Systems Biology 2023Quote: ... Mouse splenic B cells were isolated using the EasySepTM Mouse B cell isolation Kit (STEMCELL technologies, 19854) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated from mouse spleens using EasySep mouse B cell isolation kit (Stemcell Technologies, Vancouver, Canada) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... The mouse B cell isolation kit and mouse naïve CD4+ T cell isolation kit were from STEMCELL technologies. The purified rat anti-mouse α4β7 (DATK32 ...
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bioRxiv - Cell Biology 2024Quote: B cells were isolated from mouse spleens using EasySep Mouse B Cell Isolation Kit (Stem Cell Technologies 19854) and attached to poly-D-lysine coated coverslips at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: Mouse hepatic progenitor organoids (70932, STEMCELL Tech) were seeded in 96 well plate using 20ul Matrigel (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-Nestin (STEMCELL Technologies; 1:100); Rabbit anti-GFAP (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg/ml mouse heparin (Stemcell technologies), 1X Pen/strept (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg/ml mouse heparin (Stemcell technologies), 1X Pen/strept (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... in mouse Mesencult Expansion medium (StemCell Technologies). After 10 days in culture ...
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bioRxiv - Immunology 2023Quote: B cells were isolated from naïve mouse spleens with EasySepTM Mouse Pan-B cell Isolation Kit (Stem Cell Technologies) and suspended in Opti-MEM™ I Reduced Serum Medium (Gibco) ...
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bioRxiv - Immunology 2023Quote: B cells were isolated from naïve mouse spleens with EasySepTM Mouse Pan-B cell Isolation Kit (Stem Cell Technologies) and stimulated following the same method as BCR signal analyses described above ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated from naïve mouse spleens with EasySepTM Mouse Pan-B cell Isolation Kit (Stem Cell Technologies) and suspended at 1 x 106 cells/mL in the supplemented RPMI1640 (Gibco) ...
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bioRxiv - Immunology 2021Quote: Naïve CD8 T cells were isolated from young female (6w-8w) B6 mouse lymph nodes and spleens with the EasySep Mouse Naïve CD8 T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... plus mouse NeuroCult proliferation supplements (Stem Cell Technologies) added with 2 µg/ml heparin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-Beta Tubulin (STEMCELL Technologies; 1:500); Rabbit anti-Olig2 (PhosphoSolutions ...
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bioRxiv - Immunology 2023Quote: IntestiCult Organoid Growth Medium (mouse) (Stem Cell Technologies) was used to establish and culture mouse intestinal organoids ...
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bioRxiv - Genomics 2020Quote: ... Lymph nodes were harvested from a 6-week-old GFP OT-1 mouse and CD8 T cells were isolated using an EasySep mouse CD8 T cell negative selection kit (StemCell Technologies). 14 days post tumor injection ...
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bioRxiv - Molecular Biology 2023Quote: CD4+ cells were isolated from freshly dissociated mouse spleen using the EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, 19852). CD4+ cells were then immediately used for bulk ATAC-seq ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse T-cells were isolated from mouse PP/lymph nodes/spleens and tumors using EasySep Mouse CD8+ T Cell Isolation kit (StemCell Technologies). For FACS analysis ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... we added IntestiCult Mouse Organoid Growth Medium (STEMCELL Technologies) to the wells ...
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bioRxiv - Genetics 2022Quote: ... Anti-nestin mouse antibody (StemCell Technologies, 60091, 1:1000), Alexa Fluor 488-anti-Mouse (A11029 ...
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bioRxiv - Immunology 2019Quote: ... or EasySep™ mouse neutrophil enrichment kit (StemCell technologies) as previously described (Park et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse methylcellulose complete media (Stem Cell Technologies, Vancouver, BC) was used to assay hematopoietic colony formation ...
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bioRxiv - Bioengineering 2023Quote: ... using the mouse T cell isolation kit (StemCell Technologies), and following the manufacturer’s instructions ...