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Citations for Stemcell Technologies :
1 - 50 of 713 citations for Breast Cancer Type 1 Susceptibility Protein BRCA1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Breast cancer cells were cultured in MammoCult (Stemcell Technologies) on non-TC treated flasks to form mammospheres ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated Lin− breast cancer cells were cultured in Human EpiCult™-B Medium Kit medium (Stem Cell Technologies, Vancouver, Canada) containing a supplement mix ...
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bioRxiv - Cancer Biology 2020Quote: Cancer stem cell activity was measured using the ALDEFLUOR assay (Stem Cell Technologies). This fluorescent reagent system allows detecting the activity of aldehyde dehydrogenase (ALDH) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Pooled breast epithelial single cells from 15 patients were cultured in MammoCult media (Stem Cell Technologies, Vancouver, Canada) and treated with curcumin ...
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bioRxiv - Bioengineering 2023Quote: ... to 1 volume of CellAdhere™ type I bovine (STEMCELL Technologies, Cat# 07001) we added 8/10 volume of DMEM ...
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bioRxiv - Genomics 2019Quote: ... and for 1 h at 4 0C with a combination of conjugated antibodies to surface proteins TRA-1-60 (StemCell Technologies, 60064PE), TRA-1-81(StemCell Technologies ...
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bioRxiv - Immunology 2019Quote: ... Collagenase Type IV (STEMCELL Technologies, Catalog #07909); ROCKi ...
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bioRxiv - Bioengineering 2022Quote: ... printed constructs were incubated for 20 min in 5 mg mL-1 collagenase type I (STEMCELL Technologies) in PBS supplemented with 5% FBS and then lysed with RLT buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Roughly separated tubules were incubated in collagenase type IV in DMEM (5 mL for 1 testis pair, 1 mg/mL, STEMCELL Technologies Inc., Cat# 07909) at 37 °C while rotating ...
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bioRxiv - Bioengineering 2023Quote: ... 0.5 mg/ mL Collagenase Type IV (Stem Cell Technologies), 250 U/ mL Deoxyribonuclease I (Worthington Biochemical Corporation ...
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bioRxiv - Bioengineering 2023Quote: ... 0.5 mg/mL Collagenase Type IV (Stem Cell Technologies), 250 U/mL Deoxyribonuclease I (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2020Quote: ... we thawed frozen viable breast cancer pleural effusions or ascites from patients and depleted dead cells with EasySep™ Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies) and CD45+ cells with EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were treated with Collagenase Type I (Stemcell technologies; 07902) at 0,125% for 1h at 37º ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 μg/ml collagen type I (Stemcell Technologies, Catalog # 07001), 50 μg/ml penicillin/streptomycin ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were dissociated with Collagenase Type IV (STEMCELL Technologies, Catlog #07909). Differentiation media contain growth factors and small molecule including SHH (100 ng/ml ...
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bioRxiv - Genetics 2022Quote: ... Anti-nestin mouse antibody (StemCell Technologies, 60091, 1:1000), Alexa Fluor 488-anti-Mouse (A11029 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated in collagenase type IV followed by Accutase (Stemcell Technologies, BC, Canada) and the fragmented cells were passaged to a new collagen-coated plate.
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bioRxiv - Cell Biology 2024Quote: ... or 1:20 Alexa488-conjugated anti-TRA-1-60 antibody (7.5 μg/mL final concentration; STEMCELL Technologies). The cells were stained in 100 μL of diluted antibody per 106 cells overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Antibodies used in immunofluorescence: Anti-Mouse OCT4 (STEMCELL TECHNOLOGIES, 60059, 1:200); Rabbit Monoclonal Anti-Sox9 (abcam ...
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bioRxiv - Physiology 2022Quote: ... Isolated PKJ tissues were then enzymatically digested with collagenase type IV (1mg/ml, Stemcell Technologies) for one hour at 370C ...
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bioRxiv - Neuroscience 2024Quote: ... the control antibody was added (0.2 µg/µl, 1:3390, STEMCELL Technologies, 60070AD). Antibodies were incubated for 10 min at 4 °C in the dark ...
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bioRxiv - Immunology 2020Quote: Specific cell types were isolated from the PBMC fraction using the following kits (STEMCELL Technologies Inc.) per manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Macroscopic lung nodules were dissected separately and digested in Collagenase Type IV (Stem Cell technologies; 07909) at 37°C for 1.5 h with rocking ...
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bioRxiv - Neuroscience 2021Quote: ... Isotype control was stained using control antibody (STEMCELL Technologies, 60070AD.1, 0.2 ug/ul) at 1:378 dilution for 10 min at 4 °C protected from light ...
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bioRxiv - Bioengineering 2023Quote: ... The following primary antibodies were used: beta-tubulin III (STEMCELL Technologies 60052; 1:1000), GFAP (Dako GA524 ...
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bioRxiv - Molecular Biology 2021Quote: Cells from E10.5 Dot1l-/- and wild-type yolk sacs were cultured in M3434 methylcellulose medium (StemCell Technologies) containing the cytokines SCF ...
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bioRxiv - Immunology 2022Quote: ... coated with human type IV placental collagen in submerged PneumaCult-Ex Plus media (StemCell Technologies, Vancouver, Canada). When cells reached confluency ...
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bioRxiv - Physiology 2023Quote: ... Homogenized livers were resuspended in 10mL HBSS containing 0.05% collagenase (Type IV; Stem cell technology; cat#07427) and the specimens were shaken for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies), irradiated (20 Gy ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5-7 days and embedded into 2 mg/ml collagen type-I (StemCell Technologies, Vancouver, Canada) gels ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Biochemistry 2019Quote: ... Embryoid bodies (EBs) were generated from feeder-free iPSCs by incubating colonies with Collagenase Type IV (7909, StemCell Technologies) for 10 min ...
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bioRxiv - Immunology 2021Quote: ... Both cell types were passaged every 7 days or as they reached 70-80% confluency using ReLeSR (STEMCELL Technologies). All iPSC and ESC lines used for these studies are listed in Table 1 along with the method of reprogramming and institution they were reprogrammed at ...
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bioRxiv - Bioengineering 2023Quote: ... 1G4 PBMCs loaded onto pMHC nanovials were sorted based on calcein AM and NGFR signal and reconstituted with 0.75 mL media and 0.25 mL of 10 mg/mL Collagenase Type II solution (STEMCELL Technologies), followed by a 2 hour incubation at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 µM A83-01, an inhibitor of TGFβ type I receptors (ALK4, ALK5, ALK7) (Stem Cell Technologies, 72022) for 5 days in vitro to induce embryoid body (EB ...
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bioRxiv - Neuroscience 2020Quote: ... carrying genes for tdTomato and the murine cell-surface protein CD90.2/Thy1.2 driven by the endogenous POU4F2 (BRN3B) promoter were clonally propagated in mTeSR-1 media (StemCell Technologies, Cambridge, MA) on growth factor-reduced Matrigel substrate (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were re-plated in mTeSR1 medium supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific) and 10 mM Rho-associated protein kinase (ROCK) inhibitor (Stemolecule Y-27632, STEMCELL Technologies) for 24 hr ...
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bioRxiv - Developmental Biology 2021Quote: ... c-Kit+) from neonatal wild-type mice were FACS sorted and cultured in MethoCultTm medium (GF M3434, Stem cell technologies) in 35mm dishes ...
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bioRxiv - Immunology 2022Quote: ... CD19+ cells were purified from C57BL/6 wild-type mice using the EasySep magnetic separation system (Stemcell Technologies, Vancouver, Canada) and were activated with lipopolysaccharide (10 μg/mL ...
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bioRxiv - Genomics 2021Quote: Wild type SB AD3.1 [21] and AD/N3HAV lines were maintained as undifferentiated hiPSC in mTeSR1 medium (Stem Cell Technology) on 1:30 diluted Matrigel (hESC grade ...
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bioRxiv - Bioengineering 2024Quote: ... each well of the 96 well plate was first coated with 45 μL 2.25% Type I Bovine Collagen Solution (StemCell Technologies) for 1 hour at 37 C ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
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bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... and plated onto low attachment V-type 96-well plate at 20 000 cells per well or AggreWell800 plate (Stemcell Technologies) to form aggregates ...
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bioRxiv - Genetics 2022Quote: ... Human ESCs (wild type and ADAR1 KO, gifts from Dr. C. Rice, the Rockefeller University) were maintained in complete mTeSR1 (StemCell Technologies) and the differentiated neural progenitor cells were maintained in STEMdiff Neural Progenitor Medium (StemCell Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... we immunomagnetically isolated natural Treg from wild type female mice or Foxp3-RFP male and female mice using a CD4+ and CD25+ Treg isolation kit (STEMCELL Technologies) as described below ...
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bioRxiv - Cancer Biology 2023Quote: Primary mouse NK cells were isolated from spleens of 8-12-week-old wild-type or Prf1-/- mice using the negative selection EasySep mouse NK cell isolation kit (STEMCELL Technologies) or positive selection CD49b (DX5 ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...