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Citations for Stemcell Technologies :
1 - 50 of 1931 citations for Anti CD25 SAP human Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD25-PE-Cy7 (clone MA251) plus FVD-eFluor-780 (eBioscience) and human FcR blocking Reagent (StemCell Technologies). Cells were washed then fixed and permeabilized using the eBioscience FOXP3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Neuroscience 2023Quote: ... CD4+ cells were subjected to CD25+ cell isolation (STEMCELL Technologies). Cells were resuspended at 106 cells per 200μl saline and injected i.p ...
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bioRxiv - Immunology 2023Quote: ... one piece (∼3g) was used for CD25+CD8- Treg enrichment using a custom thymic Treg selection kit (STEMCELL Technologies)102 ...
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... we immunomagnetically isolated natural Treg from wild type female mice or Foxp3-RFP male and female mice using a CD4+ and CD25+ Treg isolation kit (STEMCELL Technologies) as described below ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... EasySep human CD4+ T cell enrichment kit and EasySep human CD8+ T cell enrichment kit (STEMCELL Technologies, Canada), respectively ...
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bioRxiv - Immunology 2021Quote: Cells were sequentially enriched for CD4+ and CD25+ cells by RosetteSep (STEMCELL Technologies) and CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2023Quote: ... magnetically isolated rCD4 (CD3+/CD4+/CD69-/ CD25-/ HLA-DR-) T cells (Stemcell Technologies) were rested in the presence of antiretroviral drugs (10nM Raltegravir and 20nM Efavirenz) ...
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bioRxiv - Cell Biology 2024Quote: ... An antibody to CD25-PE (Clone 2A3, #60153PE) was obtained from StemCell Technologies. Antibodies to SOX2 (#MAB2018) ...
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bioRxiv - Neuroscience 2023Quote: ... Isolated CD4+ T cells were then subjected to a CD25+ cell isolation (STEMCELl Technologies) using releasable magnetic spheres (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... CellSep Human CD19+ selection kit (Stem Cell Technologies) was used to enrich for CD19+ cells ...
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bioRxiv - Immunology 2023Quote: ... EasySep Human CD34+ Positive Selection Kit (Stemcell Technologies) was used to enrich for CD34+ cells to >90% purity.
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bioRxiv - Immunology 2023Quote: ... and EasySep Human Monocyte Isolation Kit (StemCell Technologies). In the coculture experiment ...
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bioRxiv - Cancer Biology 2022Quote: ... human leukemia cells were isolated using EasySepTM Mouse/Human Chimera Isolation Kit (#19849; Stemcell Technologies). All animal experiments were approved by and performed in accordance with guidelines of the Institutional Animal Care and Use Committee at University of Colorado (protocol No ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... or anti-human CD32 (FcγRII Blocker, StemCell Technologies) for 15 minutes on ice to block non-specific staining and then incubated with fluorochrome-conjugated antibodies for 30 minutes on ice and protected from light ...
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bioRxiv - Cell Biology 2020Quote: . Primary human CD3+ and CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human T cell Isolation Kit and Human CD8 T cell isolation kit with RapidSpheres (Stemcell Technologies). Mobilized human primary CD34+ cells from Peripheral Blood (were obtained from Caltag Medsystems ...
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bioRxiv - Bioengineering 2020Quote: ... CD4pos and CD8pos T cells were isolated by negative selection using EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells and CD25hi CD4+ T cells were negatively isolated from PBMCs separately by using Easysep human CD4+ T cell isolation kits and EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells and CD8+ T cells were enriched using the EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2021Quote: ... human neutrophils were purified from blood using the EasySep™ Human Neutrophil Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: Human Monocyte were acquired from frozen PBMCs with STEMCELL EasySep Human Monocyte Enrichment kit (STEMCELL TECHNOLOGIES). Purified monocytes were cultured in RPMI-1640 Glutamax medium (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Human pDC isolation was performed using an EasySep™ Human Plasmacytoid DC Enrichment kit (STEMCELL Technologies) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... and the EasySep human monocyte isolation kit (StemCell Technologies) with equivalent results ...
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bioRxiv - Biochemistry 2022Quote: ... with EasySep Human T cell Isolation Kit (STEMCELL Technologies). Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... or Human T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI 1640 (ATCC ...
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bioRxiv - Immunology 2024Quote: ... 1.5 µg/mL anti-human CD32 antibody (STEMCELL Technologies) and 10 µg/mL anti-mouse CD16/CD32 antibody (as needed ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells or CD56+CD3-NK cells were isolated from the PBMC population using the EasySep Human T Cell Isolation Kit or EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... CD8+ and CD3+ T cells were isolated from PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit and EasySep™ Human T Cell Isolation Kit (both from STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2022Quote: B cells were enriched from human PBMCs using EasySep Human Pan-B Cell Enrichment Kit (StemCell Technologies) before being incubated with Fc block (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were isolated using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Human CD14+ monocytes were isolated from leukopaks using the EasySep Human CD14+ Positive Selection Kit (STEMCELL Technologies) and differentiated into monocyte-derived dendritic cells (moDCs ...
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bioRxiv - Synthetic Biology 2022Quote: Primary CD4+ and CD8+ T cells were isolated from blood of anonymous donors by negative selection using the Human CD4+ T cell isolation kit and Human CD8+ T cell isolation kit (STEMCELL Technologies #17952 and #17953). T cells were cryopreserved in RPMI1640 (UCSF cell culture core ...
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bioRxiv - Microbiology 2022Quote: ... EasySep™ Human CD14 Positive Selection Kit II (StemCell Technologies) was used ...
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bioRxiv - Immunology 2020Quote: EasySep™ Direct Human NK cell Isolation Kit (StemCell Technologies);
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bioRxiv - Neuroscience 2022Quote: ... EasySep Negative Selection Human Monocyte Enrichment kits (Stemcell Tech, Inc.), without CD16 depletion ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented NeuroCult NS-A proliferation kit (human, STEMCELL Technologies, #05751), human recombinant EGF (20 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... with EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies). Enriched CD4+ cells were stained for 30 min on ice with antibodies against anti-CD4 ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... EasySep™ Human NK cell-negative isolation kit (StemCell Technologies) was used to isolate human peripheral blood NK cells from healthy donor leukopaks ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... EasySep™ Human T-cell-negative isolation kit (StemCell Technologies) was used to isolate peripheral blood T cells from healthy donor leukopaks ...
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bioRxiv - Immunology 2024Quote: ... using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies). The isolated NK cells were incubated overnight at 37°C 5% CO2 in R10 (RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... 1 × 105 human NK cells isolated from PBMC using EasySep human NK cell isolation kit (Stem Cell Technologies) were added to wells in the presence of CD107a PE (BD ...
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bioRxiv - Cell Biology 2022Quote: ... human neutrophils were isolated from whole blood using the EasySep Direct Human Neutrophil Isolation kit from Stemcell Technologies by immunomagnetic negative selection ...
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bioRxiv - Cancer Biology 2022Quote: CD8+ cells were enriched from human PBMCs with EasySep Human CD8+ T Cell Isolation Kit (Stem Cell Technologies) and stimulated with 5 ug/mL Ultra-LEAF anti-human CD3 (Biolegend ...