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Citations for Stemcell Technologies :
1 - 50 of 591 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Following enumeration using 3% acetic acid with methylene blue (StemCell Technologies), splenocytes were resuspended in phosphate-buffered saline and forty to sixty million cells were injected either intraperitoneally or intravenously (via retro-orbital injection under isoflurane anesthesia ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3–5 days with ReLeSR (STEMCELL Technologies). All cells used had a normal diploid karyotype.
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 min followed by a 3 min treatment with 5 mg/mL dispase (Stem Cell Technologies, 07913) and 1 mg/mL DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and DNaseI (9003-98-9, STEMCELL Technologies) for 40 min at 37°C in complete HBSS (21-023-CV ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
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bioRxiv - Molecular Biology 2023Quote: ... 9 μL Buffer R2 (STEMCELL Technologies, 100-0691), 1 μL 30 μM of gRNA (Table 2) ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... IL-9 and TPO (02696, STEMCELL Technologies, Canada) for induction of megakaryopoiesis ...
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bioRxiv - Neuroscience 2021Quote: ... with 10 μM Y-23632 (only for the initial 24 hours; Wako) and subcultured every 5–7 days using ReLeSR reagent (STEMCELL Technologies).
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured in a Cytomat 24 (Thermo Fischer Scientific) at 37°C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies), supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific).
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... The obtained pellet is then washed and resuspended in cold RPMI media (Wisent Bioproducts. Purity and viability of the obtained neutrophils were verified through Methylene blue (Stem cell Technologies) and Trypan blue (Wisent ...
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bioRxiv - Genetics 2019Quote: ... Cells were rinsed with PBS (5 mL) and treated with 3 mL Accutase (STEMCELL technologies) for 5 min at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... The microcapsules carrying HUES-8 cells were collected into 15 mL tube filled with 5 mL E8 medium (STEMCELL Technologies), then distributed into a 6 well-plate at density of 2000 capsules/well and incubated at 37 □ with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were collected by centrifugation at 1300 rpm for 5 minutes and 500 K cells distributed into each well of an AggreWell™800 plate (24 well) (Stem Cell Technologies) as recommended by the manufacturer.
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
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bioRxiv - Immunology 2024Quote: Female CatchupIVM-red and FVB/N mice aged 8-15 weeks were treated with 5 µg mouse recombinant G-CSF (STEMCELL Technologies) by intraperitoneal injection (i.p. ...
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bioRxiv - Molecular Biology 2020Quote: ... or β-thalassemia patients were cultured for 14-16d at 37°C and 5% CO2 in SFEM II medium (STEMCELL Technologies, Vancouver, Canada) as previously described37,38 ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... After 3 days of growth of naïve PSC were harvested and counted and 55 cells were seeded per microwell (total of 66,000 cells were seeded per 24-well in 1 ml of medium) AggreWell 400 24-well (Stemcell Technologies cat 34415) in N2B27-BSA supplemented with 10 µM ROCKi Y27632 ...
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bioRxiv - Developmental Biology 2022Quote: A 24-well AggreWell™400 plate (Stemcell Technologies) was first prepared following the manufacturer’s anti-adherence treatment recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... An Aggrewell-800 24-well plate (StemCell Technologies, #34811) was pre-washed with Anti-Adherence Rinsing Solution (StemCell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... AggreWell™ 400 24-well Plates (STEMCELL Technologies, 34415) were prepared following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872). All cell cultures were maintained in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich], and 10 uM Y27632 [72304, StemCell Technologies] in mTeSR1), and centrifuged at 3200 xg for 30 to 90 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... 9 x 106 cells were seeded into 6-well Aggrewell 800 plates (StemCell Technologies) in embryoid body media consisting of complete StemFlex supplemented with 10 μM Y27632 (Stemcell Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... coated 24-well plate in mTeSR1 (STEMCELL Technologies, cat # 85870) media supplemented with 10μM of the ROCK inhibitor Y27632 (STEMCELL Technologies ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Unsorted floating cells were taken from d2-3 of the blast culture and 5×103 cells were seeded in 1 ml MethoCult (M3434 STEMCELL Technologies) per dish ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested anytime between day 9-21 using dispase (StemCell Technologies, cat. no. 07913) and trypsin to dissociate Matrigel and spheres ...
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bioRxiv - Immunology 2020Quote: ... 8 weeks old males (Stem Cell Technologies #19765), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in 24- well plates in SFEM (Stem Cell Technologies) plus 100 ng ml−1 mTPO (Novoprotein ...
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bioRxiv - Bioengineering 2022Quote: ... and then aggregated in AggreWell 800 24-well plates (STEMCELL Technologies) at a density of 3,000,000 cells per well with 2mL of AggreWell Medium (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
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bioRxiv - Neuroscience 2022Quote: ... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ...
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bioRxiv - Cell Biology 2020Quote: ... Neural rosettes were derived between day 3 to day 5 by addition of STEMdiffTM Neural Rosette Selection Reagent (05832, Stem Cell Technologies) for 45 mins-1 hr at 37oC ...
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bioRxiv - Bioengineering 2022Quote: ... 10 μM TGF-β pathway inhibitor (SB431542, Stem cell technology, #72234) and ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... Wells of a 24-well AggreWell™ 400 plate (STEMCELL Technologies, #34411) were seeded with either 1:1 iEndo-mKate2:WT-eGFP cells in EBCM with 10 μM Y-27632 ...
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bioRxiv - Bioengineering 2020Quote: ... Wells of a 24-well AggreWell™ 800 plate (STEMCELL Technologies, #34815) were treated with an anti-adhesive coating of 0.2% Pluronic F127 in PBS and seeded with 1.5×106 cells/well using either WT PGP1 hiPSCs or a 1:2 ratio of iEndo:WT PGP1 hiPSCs in EBCM supplemented with 10 μM Y-27632 ...
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bioRxiv - Developmental Biology 2023Quote: ... media was switched to RSeT media after 24 hours (05978, STEMCELL Technologies). Cells were maintained in RSeT and passaged as above every 4-5 days ...
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bioRxiv - Neuroscience 2024Quote: Commercially available microwell plates (AggreWellTM 400 24-well plate, 34415, StemCell Technologies) were used to generate retinal and thalamic spheroids (250 ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... cells were grown in 24 well AggreWell 400 plates (Stemcell Technologies, 34415). Each well was washed once with 1x PBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by expansion for 9 days in Expansion Media composed of Stemspan H3000 (Stem Cell Technologies, 09850) supplemented with 50μg/ml ascorbic acid (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with phosphate-buffered saline (PBS) (5 ml) and detached by addition of ‘Gentle Dissociation Reagent’ (3 ml) (STEMCELL Technologies; Cat. No. 07174) stored at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... the isolated intestinal tissue was cut into ∼3-to 5-mm pieces and incubated in Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada). After removal of the Gentle Cell Dissociation Reagent ...