Labshake search
Citations for Stemcell Technologies :
1 - 50 of 1159 citations for 6 Chloro 2 piperazino 1 3 benzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2024Quote: 2-3 days post-electroporation HSPCs were plated in SmartDish 6-well plates (cat.: 27370; STEMCELL Technologies, Vancouver, Canada) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat. ...
-
bioRxiv - Neuroscience 2019Quote: ... with medium changes every 2-3 days and passages 1-2 times per week using ReLeSR (Stem Cell Technologies). Heteroplasmy levels were regularly measured to insure they were retained across passage numbers ...
-
bioRxiv - Genomics 2021Quote: ... onto Matrigel-coated (1 mg ml-1) 6-well plates 6 using ReLeSR (STEMCELL Technologies). Cells were differentiated to CD43+ hematopoietic progenitors using the StemCell Technologies Hematopoietic Kit (Cat #05310) ...
-
bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
-
bioRxiv - Neuroscience 2023Quote: ... 3 × 10^6 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form embryoid bodies (EBs) ...
-
bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Stem Cell Technologies). Cytological analysis of blood and bone marrow smears from Inpp4b+/+ and Inpp4b-/- leukemic mice was done by classical wright-giemsa staining (Sigma) ...
-
bioRxiv - Developmental Biology 2020Quote: ... fixed in Methacarn (1/3/6 mixture of acetic acid/chloroform/methanol) overnight at room temperature and stained with carmine alum (Stem Cell Technologies), or fixed in 4% paraformaldehyde overnight at 4°C ...
-
bioRxiv - Immunology 2021Quote: Female C57BL/6 mice received a single dose of mouse recombinant CSF-3 (StemCell Technologies) at 0.25mg/kg in sterile PBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
-
bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
-
A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 2-3 days using accutase (Stemcell Technologies, #07920). Cells were then collected by centrifugation at 1200 rpm for 3 minutes and counted before seeding ...
-
bioRxiv - Bioengineering 2022Quote: ... and interleukin-3 (IL-3; 1 ng/mL, first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL by daily feeding with fresh expansion medium.
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged 1:6 using RELESR (StemCell Technologies 05872) for detachment of hESCs ...
-
bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
-
bioRxiv - Bioengineering 2021Quote: ... All human iPSCs were cultured on Matrigel (Corning)-coated 6-well plates with mTeSR™1 medium (StemCell Technologies). Full-volume medium replacement with mTESR1 was performed daily ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). Cells were cryopreserved in mFreSR.
-
bioRxiv - Developmental Biology 2021Quote: Unsorted cells at day 6 (4+2) of differentiation were cultured in MethoCult 04434 (Stem Cell Technologies) in 35 mm dishes ...
-
bioRxiv - Genomics 2019Quote: ... They were passaged using 1:3 Accutase (STEMCELL Technologies).
-
bioRxiv - Cell Biology 2021Quote: ... 1 μM PD03259010 and 3 μM CHIR99021 (STEMCELL Technologies) and 100 U/ml penicillin ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
-
bioRxiv - Genomics 2022Quote: ... 1000 cells of each sorted population were seeded in 1 mL of methylcellulose medium supplemented with recombinant murine SCF and IL-3 with recombinant human IL-6 and Epo (M3434, STEMCELL Technologies) in duplicate ...
-
bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
-
bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
-
bioRxiv - Cancer Biology 2021Quote: ... For the first 2 days cells were also exposed to 3 μM CHIR-99021 (Stem Cell Technologies). From day 4 for 5 days ...
-
bioRxiv - Cell Biology 2023Quote: ... Sorted cells were plated in Methocult optimum media (2-3 X 103 cells/well; Stemcell Technologies, H4034) according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Split NPCs 1:3 every week with accutase (STEMCELL Technologies; 7920). NPCs were further differentiated into human astrocytes by seeding dissociated single cells at 15,000 cells/cm2 density on Matrigel-coated plates in Astrocyte medium (ScienCell ...
-
bioRxiv - Neuroscience 2021Quote: ... the cells were counted and 2 million were cultured for 6 days in hematopoietic expansion media -Stemspan H3000(Stemcell Technologies), with the addition of EPO (R&D) ...
-
bioRxiv - Neuroscience 2021Quote: ... Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival ...
-
bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Two days post-transfection, the cells were replated in a 6 well (Falcon, 353224) and selected in 2 μg/ml Puromycin (STEMCELL Technologies). Every two days ...
-
bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1.1ml of the mixture (2 x 104 BM cells and 2 x 105 spleen cells) was plated in triplicate into 6-well dishes (SmartDishTM, STEMCELL Technology). After incubation for 10 days ...
-
bioRxiv - Cell Biology 2019Quote: ... with a full medium change at day 2 and passage at day 3 using Accutase (STEMCELL Technologies, 07920).
-
bioRxiv - Genomics 2021Quote: ... 25 000 cells were sorted into IntestiCult Organoid Growth Medium (0600-0, −2 and −3 combined, STEMCELL Technologies), centrifuged and directly processed for library generation with the 10x Genomics technology according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were seeded at a 1:6 ratio and routinely passaged with Dispase (Stemcell Technologies).
-
Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... cells were passaged at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured in NPC medium on Matrigel coated plates onwards.
-
bioRxiv - Cell Biology 2023Quote: ... final concentrations of either 3 µM CHIR99021 for definitive hematopoietic differentiation or 3 µM IWP2 (Selleckchem, S7085) and 1 ng/ml activin A (Stemcell Technologies, 78001) for primitive hematopoietic differentiation were added to the media for 30 hours ...
-
bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... The media was changed daily and the iPSC cultures were split into 1:6-1:10 every 5 days using ReLeSR (Stemcell Technologies).
-
bioRxiv - Bioengineering 2023Quote: ... 2.5 g of Hydrosil silicone components (1:1 wt) (101301; SILADENT) was added into each well of 6-well microwell culture plate (AggreWellTM400; STEMCELL Technologies) followed by centrifugation at 55 x g for 1 min and curing at 60 °C for 1 h ...
-
bioRxiv - Neuroscience 2024Quote: ... in a feeder free fashion and passaged every 4∼5 days at a 1:4∼1:6 ratio using ReLeSR (Stemcell technologies) as described previously 88,95 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3 days post-transfection in 1 ml STEMdiff Neural Progenitor Medium (Stemcell Technologies) cells were washed by DPBS and 2 wells of the plate were put together and collected by centrifugation at 10.000 rpm for 2 min generating 3 biological replicates ...
-
bioRxiv - Systems Biology 2023Quote: ... we passaged the cells at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured cells in NPCs medium onwards.
-
bioRxiv - Systems Biology 2023Quote: ... Cells were passaged 1:10 every 3 days using ReLeSR (StemCell Technologies #05873). Cells were maintained in a humidified incubator at 37°C with 5% CO2.
-
bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
-
bioRxiv - Developmental Biology 2021Quote: Cells were plated at 2 days after cell sorting (Day 6 post differentiation) into a methylcellulose-based medium containing hematopoietic cytokines (MethoCult M3434, StemCell Technologies, Cat. 03434) at 6000 cells/ml ...