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1 - 50 of 308 citations for 3 4 Dehydro Cilostazol since 2019

• Myogenesis defects in a patient-derived iPSC model of hereditary GNE myopathy

  Rebecca E Schmitt, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Passaging occurred every 3-4 days using ReLeSR (STEMCELL Technologies) and DPBS 1X (Gibco ...
• Optimization and scaling of patient-derived brain organoids uncovers deep phenotypes of disease

  Kevan Shah, et al., bioRxiv - Neuroscience 2020

  Quote: ... and banked into 3-4 vials using mFreSR (Stemcell Technologies). Clearance of Sendai virus was confirmed using qPCR (Supplementary Table 4) ...
• Personalized chordoma organoids for drug discovery studies

  Ahmad Al Shihabi, et al., bioRxiv - Cancer Biology 2021

  Quote: ... in a 3:4 mixture of media (StemCell Technologies # 05620) and Matrigel (BD Bioscience CB-40324) ...
• Personalized chordoma organoids for drug discovery studies

  Ahmad Al Shihabi, et al., bioRxiv - Cancer Biology 2021

  Quote: ... in the same 3:4 mixture of media (StemCell Technologies #05620) and Matrigel (BD Bioscience CB-40324) ...
• Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease relevant pathologies

  Nadeem Murtaza, et al., bioRxiv - Neuroscience 2022

  Quote: ... and passaged every 3-4 days using ReLeSR (Stem Cell Technologies) until neural induction ...
• Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease relevant pathologies

  Nadeem Murtaza, et al., bioRxiv - Neuroscience 2022

  Quote: ... and passaged every 3-4 days using ReLeSR (Stem Cell Technologies). Human iPSCs were edited for homozygous knockout of TAOK2 or heterozygous knock-in of the A135P mutation as described in Deneault et al.(Deneault et al. ...
• Single-cell Individual Complete mtDNA Sequencing Uncovers Hidden Mitochondrial Heterogeneity in Human and Mouse Oocytes

  Chongwei Bi, et al., bioRxiv - Genomics 2020

  Quote: ... The cells were passaged every 3–4 days using Accutase (Stemcell Technologies). The iPS cells conversion to EPS as previous reported47.
• An iPSC-derived small intestine-on-chip with self-organizing epithelial, mesenchymal and neural cells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... Passaging was performed every 3-4 days using ReLeSR (Stemcell Technologies, #05872) according to manufacturer’s instructions ...
• High-speed live cell interferometry for screening bioprinted organoids

  Peyton J. Tebon, et al., bioRxiv - Bioengineering 2021

  Quote: ... single cells suspended in a 3:4 mixture of Mammocult (StemCell Technologies 05620) and Matrigel (Corning 354234 ...
• Allele-specific gene editing rescues pathology in a human model of Charcot-Marie-Tooth disease type 2E

  Carissa M. Feliciano, et al., bioRxiv - Genetics 2021

  Quote: ... iPSC cultures were passaged every 3-4 days with Accutase (Stem Cell Technologies, 07920) or ReLeSR (Stem Cell Technologies ...
• The landscape of drug sensitivity and resistance in sarcoma

  Ahmad Al Shihabi, et al., bioRxiv - Cancer Biology 2023

  Quote: Primary cells are resuspended in a 3:4 solution of Mammocult medium (Stem Cell Technology) and Matrigel (Corning)17,26,29 ...
• Highly-parallel production of designer organoids by mosaic patterning of progenitors

  Catherine M. Porter, et al., bioRxiv - Bioengineering 2023

  Quote: ... and passaged every 3-4 days using Gentle Cell Dissociation Reagent (STEMCELL Technologies, 100-0485) for clump passaging ...
• Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies

  Mathieu C. Husser, et al., bioRxiv - Cell Biology 2024

  Quote: ... The cells were passaged every 3-4 days by dissociating them using Accutase (STEMCELL Technologies) according to manufacturer’s protocol ...
• Functional Integration of 3D-Printed Cerebral Cortical Tissue into a Brain Lesion

  Yongcheng Jin, et al., bioRxiv - Bioengineering 2022

  Quote: ... on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
• Spin∞ an improved miniaturized spinning bioreactor for the generation of human cerebral organoids from pluripotent stem cells

  Alejandra I. Romero-Morales, et al., bioRxiv - Bioengineering 2019

  Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (Stem Cell Technologies). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
• A transient modified mRNA encoding Myc and Cyclin T1 induces cardiac regeneration and improves cardiac function after myocardial injury

  Aleksandra Boikova, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
• An activating mutation in Pdgfrb causes skeletal stem cell defects with osteopenia and overgrowth in mice

  Hae Ryong Kwon, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
• Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR libraries

  Xander Nuttle, et al., bioRxiv - Bioengineering 2020

  Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
• Human iPSC-derived cerebral organoids model features of Leigh Syndrome and reveal abnormal corticogenesis

  Alejandra I. Romero-Morales, et al., bioRxiv - Cell Biology 2021

  Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
• Toward a Cell Culture Model of Portal Axis Lipid Handling

  Joshua R. Cook, et al., bioRxiv - Cell Biology 2022

  Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
• A Mutation-driven oncofetal regression fuels phenotypic plasticity in colorectal cancer

  Slim Mzoughi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
• Leptomeningeal Neural Organoid (LMNO) Fusions as Models to Study Meninges-Brain Signaling

  Hannah E Jones, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
• Epitope Engineered Human Haematopoietic Stem Cells are Shielded from CD123-targeted Immunotherapy

  Romina Marone, et al., bioRxiv - Bioengineering 2023

  Quote: ... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
• Human Cerebral Spheroids Undergo Activity Dependent Changes In Cellular Composition And Microrna Expression

  Thomas Parmentier, et al., bioRxiv - Neuroscience 2022

  Quote: ... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ...
• Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis

  Biren M. Dave, et al., bioRxiv - Developmental Biology 2023

  Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
• Development of an objective index, neural activity score (NAS), reveals neural network ontogeny and treatment effects on microelectrode arrays

  Austin P. Passaro, et al., bioRxiv - Neuroscience 2020

  Quote: ... Cells were maintained with media changes every 3-4 days with full neural culture media consisting of BrainPhys™ Basal Media (STEMCELL Technologies, Vancouver, BC, Canada) or Advanced DMEM/F12 (ThermoFisher ...
• Expansion and differentiation of ex vivo cultured erythroblasts in scalable stirred bioreactors

  Joan Sebastián Gallego-Murillo, et al., bioRxiv - Bioengineering 2022

  Quote: ... and interleukin-3 (IL-3; 1 ng/mL, first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL by daily feeding with fresh expansion medium.
• Base-resolution models of transcription factor binding reveal soft motif syntax

  Žiga Avsec, et al., bioRxiv - Genomics 2020

  Quote: ... 3 µM CHIR99021 (Stemcell Technologies), 1 µM PD0325901 (Stemcell Technologies) ...
• Building functional circuits in multispecies brains

  Benjamin T. Throesch, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 3 μM CHIR99021 (STEMCELL Technologies), 1 μM PD0325901 (STEMCELL Technologies) ...
• Reconstructing bat antiviral immunity using epithelial organoids

  Max J. Kellner, et al., bioRxiv - Immunology 2024

  Quote: ... + 3 µM CHIR9902 (Stemcell Technologies) + 10 µM SB-431542 (Stemcell Technologies ...
• Use of Induced Pluripotent Stem Cells to Build Isogenic Systems and Investigate Type 1 Diabetes

  Lucas H. Armitage, et al., bioRxiv - Immunology 2021

  Quote: ... and 3 mM CHIR (StemCell Technologies)) ...
• Afucosylated maternal anti-dengue IgGs are a biomarker for susceptibility to dengue disease in their infants

  Natalie K. Thulin, et al., bioRxiv - Immunology 2019

  Quote: ... clone IV.3 (STEMCELL Technologies; 60012FI); Brilliant Violet 711 conjugated anti-human FcγRIII ...
• Endogenous suppression of WNT signalling in human embryonic stem cells leads to low differentiation propensity towards definitive endoderm

  Dominika Dziedzicka, et al., bioRxiv - Cell Biology 2020

  Quote: ... 3 µM CHIR99021 (CHIR, STEMCELL Technologies) and 100 ng/mL Activin A (Biotechne) ...
• Circadian regulation of lung repair and regeneration

  Amruta Naik, et al., bioRxiv - Cell Biology 2021

  Quote: ... CHIR99021 3 µm (72052 StemCell Technologies), DMSO (final concentration 0.05% ...
• Chromatin Profiling of CBFA2T3-GLIS2 AMLs Identifies Key Transcription Factor Dependencies and BRG1 Inhibition as a Novel Therapeutic Strategy

  Samantha Kaonis, et al., bioRxiv - Cancer Biology 2023

  Quote: ... + 10ng/ml IL-3 (STEMCELL Technologies). The WSU-AML cell line was also seeded at 1x106 cells/ml ...
• Polycomb contraction differentially regulates terminal human hematopoietic differentiation programs

  A. Lorzadeh, et al., bioRxiv - Cell Biology 2020

  Quote: ... and 3 units/mL EPO (STEMCELL Technologies) in the presence of 1 µM GSK-J4 (Sigma ...
• Enhanced donor heart preservation by therapeutic inhibition of acid sensing ion channel 1a

  Meredith A. Redd, et al., bioRxiv - Physiology 2020

  Quote: ... containing 3 μM CHIR99021 (Stem Cell Technologies), 500 μg/mL BSA (Sigma Aldrich) ...
• Human bone marrow organoids for disease modelling, discovery and validation of therapeutic targets in hematological malignancies

  Abdullah O. Khan, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Interleukin-3 (IL3, StemCell Technologies, Cat#78194) and Interleukin-6 (IL6 ...
• Structurally and functionally distinct early antibody responses predict COVID-19 disease trajectory and mRNA vaccine response

  Saborni Chakraborty, et al., bioRxiv - Immunology 2021

  Quote: ... anti-CD32 (STEMCELL Technologies; clone IV.3), anti-CD32B/C (clone S18005H) ...
• IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition

  Caterina Prelli Bozzo, et al., bioRxiv - Microbiology 2020

  Quote: ... CHIR 99021 3 µM (Stemcell Technologies, 72054), Pumorphamine 1 µM (Miltenyi Biotec ...
• Modeling lung cell development using human pluripotent stem cells

  Shuk Yee Ngan, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 3 µM CHIR99021 (STEMCELL Technologies, cat#72054), 100 nM All-trans Retinoic acid (ATRA ...
• Cell cycle gene regulation dynamics revealed by RNA velocity and deep-learning

  Andrea Riba, et al., bioRxiv - Systems Biology 2021

  Quote: ... 3 µM CHIR99021 (72054, Stem Cell Technologies) and 1 µM PD0325901 (72184 ...
• Reticular Dysgenesis-associated Adenylate Kinase 2 deficiency causes failure of myelopoiesis through disordered purine metabolism

  Wenqing Wang, et al., bioRxiv - Cell Biology 2021

  Quote: ... 100μg/mL hrIL-3 (StemCell Technologies 78040), 10μg/mL hrG-CSF (NEUPOGEN ...
• Engineering human spinal microphysiological systems to model opioid-induced tolerance

  Hongwei Cai, et al., bioRxiv - Bioengineering 2022

  Quote: ... and 3 μM CHIR-99021 (Stemcell Technologies). After 4-day culture in SCM I ...
• Differentiation of airway cholinergic neurons from human pluripotent stem cells for airway neurobiology studies

  P.A. Goldsteen, et al., bioRxiv - Neuroscience 2022

  Quote: ... and 3 µM CHIR99021 (STEMCELL Technologies, 72054), was freshly added ...
• Soluble NS1 antagonizes IgG- and IgA-mediated monocytic phagocytosis of DENV infected cells

  Mitchell J. Waldran, et al., bioRxiv - Immunology 2022

  Quote: ... -32 (Stemcell Technologies 60012 clone IV.3), or -64 (Biolegend 305002 Clone 10.1).
• MAPT expression is mediated by long-range interactions with cis-regulatory elements

  Brianne B. Rogers, et al., bioRxiv - Genomics 2023

  Quote: ... 10 µg/mL NT-3 (StemCell Technologies), and 1 mg/mL Mouse Laminin (Gibco) ...
• Regulation of Store-Operated Ca²⁺ Entry by IP₃ Receptors Independent of Their Ability to Release Ca²⁺

  Pragnya Chakraborty, et al., bioRxiv - Cell Biology 2023

  Quote: ... CHIR99021 (3 μM, STEMCELL Technologies, Cat#72052), purmorphamine (0.5 mM ...
• SARS-CoV-2 and Malayan pangolin coronavirus infect human endoderm, ectoderm and induced lung progenitor cells

  Bixia Hong, et al., bioRxiv - Microbiology 2020

  Quote: ... anti-SSEA-4 antibody (STEMCELL Technologies), anti-Sox17 antibody (R&D System) ...
• Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

  Frøydis Sved Skottvoll, et al., bioRxiv - Biochemistry 2020

  Quote: ... containing 3 μM CHIR99021 (STEMCELL Technologies, Vancouver, Canada), 50 nM PI-103 from Bio-Techne Ltd ...