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Citations for Stemcell Technologies :
2501 - 2550 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... before switching to astrocyte maturation media (STEMdifftm Astrocyte maturation kit #100-0016, StemCell Technologies, Cambridge, UK). Cells were seeded to a density of 2.5×10 /cm onto tissue culture plastic on Matrigel-matrix and media was changed every other day for 7 days or until reaching 80% confluency ...
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bioRxiv - Genomics 2023Quote: ... iPSC-iCas9 cells were cultured in mTeSR (Stem Cell Technologies) supplemented with penicillin/streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte/stromal cell cultures were produced from HES3 human embryonic stem cells (hESCs, WiCell) maintained using mTeSR-1 (Stem Cell Technologies). hESCs were seeded at 2×104 cells/cm2 in Matrigel coated flasks and cultured for 4 days using mTeSR-1 and passaged with TrypLE (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was changed daily and cells were passaged using ReLeSR (Stem Cell Technologies). For differentiation ...
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bioRxiv - Neuroscience 2023Quote: ... Beta-Mercaptoethanol Thermo Fisher Scientific 21985023) with double the concentration of dual-SMAD inhibitors (8uM Dorsomorphin Sigma-Aldrich P5499, 8uM of A-83 STEMCELL Technologies 72022), initiating neural induction and termed Day 0 ...
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bioRxiv - Neuroscience 2023Quote: ... and were passaged with ReLeSR (StemCell Technologies). iPSCs were differentiated to neural progenitor cells (NPCs ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs were incubated with BrainPhys (StemCell Technologies) supplemented with 1xB27 without vitamin A ...
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bioRxiv - Neuroscience 2023Quote: ... Feeder-free hiPSCs were routinely maintained on Matrigel in mTeSR 1 medium (StemCell Technologies). Differentiation of hiPSCs to hNPCs was started with induction of neurospheres in aggrewell plates (StemCell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Differentiation of hiPSCs to hNPCs was started with induction of neurospheres in aggrewell plates (StemCell Technologies) and exposure to a cocktail of small molecules containing 2 μM each of Dorsomorphin (Tocris) ...
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bioRxiv - Neuroscience 2023Quote: ... or Papain (Stemcell Technologies, #07466) respectively according to the analytical timepoint ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in TeSR/E8 medium (Stemcell Technologies, #05990) supplemented with 100 U/mL penicillin and 100 ug/mL streptomycin (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... and treated with 0,5 mL ReLeSR (Stemcell technologies, #05872) for 5 minutes at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... IWP-2 (STEMCELL Technologies, 72124), RA (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... 2.5 μM DAPT (STEMCELL Technologies, 72082), brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Neuroscience 2023Quote: ... coated plates in mTeSR+ (Stem Cell Technologies 100-0276) and fed every day except for UNC pilot one and the first week of UNC replicate 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... CD4+ T cells were isolated by negative selection using the EasySep CD4 T cell isolation kit (Stemcell Technologies) and frozen as dry pellet at −80°C.
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bioRxiv - Molecular Biology 2023Quote: ... starting from freshly isolated PBMCs from 69 PLWH using the EasySep CD4 T cell isolation kit (Stemcell Technologies). CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... were maintained in TeSR-E8 (Stem Cell Technologies) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... For microglial differentiation iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (05310, Stem cell Technologies). HPCs were either frozen using Bambanker HRM freezing media (BBH01 ...
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bioRxiv - Neuroscience 2023Quote: ... 20ng/ml BDNF (Peprotec, STEMCELL Technologies), and 20ng/ml GDNF (Peprotec ...
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bioRxiv - Neuroscience 2023Quote: ... cortex media was supplemented with 1uM A-83 (STEMCELL Technologies), 180nM LDN193189 (STEMCELL Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... 180nM LDN193189 (STEMCELL Technologies), and 3uM IWR-1 (Millipore Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... coated plates and maintained in mTeSR+ media (STEMCELL Technologies). For routine culture ...
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bioRxiv - Neuroscience 2023Quote: ... Patterned neural spheroids were then embedded in Matrigel (StemCell Technologies, Cat. #354277) and switched to the terminal differentiation medium DMEM-F12 (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining pellet was gently resuspended in enteric glial cell media supplemented with 1x CloneR (Stem Cell Technologies, 5889) and 50ug/mL primocin (Invivogen ...
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bioRxiv - Neuroscience 2023Quote: ... or mTeSR™ Plus (Cat #05825, STEMCELL Technologies, Vancouver, BC, CAN) maintenance medium ...
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bioRxiv - Neuroscience 2023Quote: ... 20 ng/mL glial cell-derived neurotropic factor (GDNF; Cat# 78058, STEMCELL Technologies), and 200 µM L-ascorbic acid 2-phosphate sesquimagenesium salt hydrate (Cat# A8960 ...
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bioRxiv - Neuroscience 2023Quote: ... Evaluation of our iPSC cell banks with the hPSC Genetic Analysis Kit (Cat # 07550, STEMCELL Technologies) confirmed normal copy number at various mutation hotspots and assessment with a Mycoplasma PCR Detection Kit (Cat# G238 ...
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bioRxiv - Neuroscience 2023Quote: ... 2% NeuroCult™ SM1 (STEMCELL Technologies, Cat# 05711), 1% N-2 Supplement (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: NK Cells were enriched from freshly isolated PBMC using the EasySep™ Human NK Cell Isolation Kit (STEMCELL Technologies). NK cells were then counted and resuspended at 1M/mL in RPMI-1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... Frozen PBMC vials from healthy donors were purchased from STEMCELL Technologies. Beth Israel Deaconess Medical Center institutional review board approved this study ...
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bioRxiv - Genomics 2023Quote: ... The iPSC lines derived from fibroblasts were cultured on Matrigel (Corning Matrigel Matrix Basement Membrane Growth Factor Reduced)-coated dishes with mTESR1 media (StemCell Technologies) and propagated using Dispase (StemCell Technologies).
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bioRxiv - Genomics 2023Quote: Unedited and WDR90 knock-out iPSC clones were transitioned into feeder-free condition in mTeSR™ Plus medium (STEMCELL Technologies) in a matrigel-coated 6 well plate at a density of 5x105 cells/well with 5 µM ROCK Inhibitor ...
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bioRxiv - Genomics 2023Quote: ... The iPSCs were transitioned into feeder-free conditions inhouse and maintained as feeder-free cultures in mTeSR Plus basal medium supplemented with mTeSR Plus 5X supplement (StemCell Technologies). The cultures were grown in multiwell plates coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Immunology 2023Quote: T-IEL were isolated and purified using the CD8α Positive Selection kit (StemCell Technologies) following manufacturers instructions ...
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bioRxiv - Immunology 2023Quote: T-IEL were isolated and purified using the CD8α Positive Selection kit (StemCell Technologies) following manufacturers instructions ...
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bioRxiv - Genomics 2023Quote: ... with DNAse (1:10,000; Stem Cell Technologies #07900, Vancouver BC, Canada). The reaction was quenched with 10% Cosmic Calf Serum (Sigma-Aldrich #C8056 ...
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bioRxiv - Genomics 2023Quote: H9 cells were treated with 10 uM Y-27632 (Stem Cell Technologies, 72304) for at least 2 h prior to nucleofection ...
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bioRxiv - Immunology 2023Quote: ... Lymph node CD8α+ T cells were isolated and sorted using the CD8α Positive Selection Kit (Stemcell Technologies). Isolated cells were washed and resuspended at 1 x 106 cells/ml in stimulation media ...
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bioRxiv - Immunology 2023Quote: T-IEL were isolated and sorted with the CD8a Positive Selection Kit (Stemcell Technologies) and stained with Live/Dead Near-Infrared (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and Sepmate-50 (IVD) tubes (StemCell Technologies), according to manufacturer’s instructions ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Naïve CD4+ and total CD8+ T cells were isolated from the healthy donor blood using the EasySep Human Naïve CD4+ T cell or the EasySep Human CD8+ T cell isolation kit(StemCell Technologies). T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco ...
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bioRxiv - Immunology 2023Quote: ... was pelleted and resuspended in 5 mL of nycoprep (Axis Shield) or OptiPrep (STEMCELL Technologies), prepared according to the manufacturer’s directions ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... and 100µg/mL DNaseI(StemCell Technologies) in Hank’s Balanced Salt Solution with calcium(Wisent Bio Products) ...
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bioRxiv - Immunology 2023Quote: ... naive Treg cells were purified by using EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies). For human cell culture ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were purified using SepMate™ tubes (StemCell Technologies, cat.: 85450) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The resulting cell suspensions were enriched for lymphocytes using Lymphoprep (Stemcell Technologies). Following enrichment ...
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bioRxiv - Immunology 2023Quote: ... CD8+ Tn or Teff cells isolated from LmOVA-infected mice by Thy1.1 positive selection kit (StemCell Technologies, Vancouver, BC, Canada) and were cultured immediately ex vivo in Seahorse XF medium (non-buffered DMEM 1640 containing 25mM glucose ...