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Citations for Stemcell Technologies :
2251 - 2300 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... EBs were generated by seeding single-cell suspension of iPSC in AggreWell-800 plates (Stem Cell Technologies) in STEMdiff Neural Induction Medium (NIM ...
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bioRxiv - Cancer Biology 2023Quote: ... with 10-6 M hydrocortisone (07904, Stem Cell Technologies), 1% penicillin streptomycin and 1ng/mL IL- 3 (R&D) ...
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bioRxiv - Cancer Biology 2023Quote: ... 15,000-25,000 MLL-AF9 AML cells in Myelocult (M5300, StemCell Technologies) were plated and co-cultured with prelabelled MSCs for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 150-300 MLL-AF9 or LSK cells were then added on the stromal cells in Myelocult (M5300, Stem Cell Technologies) with 10-6 M hydrocortisone (07904 ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 1× mTeSR1 supplement (Stem Cell Technologies, 85850) at 37°C and 5% CO2.
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bioRxiv - Developmental Biology 2023Quote: ... Passaging was done using accutase (Stem Cell Technologies) treatment.
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bioRxiv - Cell Biology 2023Quote: ... The timely collection of the CMs was done using STEMdiff reagent from the CM dissociation kit (STEMCELL Technologies, Vancouver, BC). STEMdiff was added to each well of hiPSC-CMs ...
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bioRxiv - Cell Biology 2023Quote: Isolated islets were gently dissociated using Accutase (StemCell Technologies), followed by single-cell capture ...
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bioRxiv - Genetics 2023Quote: ... iPSC colonies were picked up and cultured in MTeSR PLUS medium (StemCell Technologies, 05825) in vitronectin (1:100 dilution ...
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bioRxiv - Genetics 2023Quote: ... The iPSCs were resuspended in STEMdiff Neural Induction Medium with the SMADi Neural Induction Supplement (STEMCELL Technologies, #08581), and 10 μm Y-27623 (Biogems ...
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bioRxiv - Bioengineering 2023Quote: ... the organoids were switched to maturation media in which BrainPhys Neuronal Media was replaced with BrainPhys Imaging Optimized Medium (Stem Cell Technologies # 5796). Calcium imaging was done using 4μM Fluo-8 AM (Abcam # ab142773) ...
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bioRxiv - Bioengineering 2023Quote: ... Dissociation and cell passages were done using ReLeSR passaging reagent (Stem Cell Technologies # 05872) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Cell freezing was done in mFreSR cryopreservation medium (Stem Cell Technologies # 05855) according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... and were differentiated for 2 days using 100 ng/ml phorbol myristate acetate (PMA) (StemCell Technologies, Cambridge, MA, USA). To study the adhesion dynamics of sickle RBCs under shear flow and hypoxia ...
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bioRxiv - Immunology 2023Quote: NK Cells were enriched from freshly isolated PBMC using the EasySep™ Human NK Cell Isolation Kit (STEMCELL Technologies). NK cells were then counted and resuspended at 1M/mL in RPMI-1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... Frozen PBMC vials from healthy donors were purchased from STEMCELL Technologies. Beth Israel Deaconess Medical Center institutional review board approved this study ...
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bioRxiv - Genomics 2023Quote: ... The iPSC lines derived from fibroblasts were cultured on Matrigel (Corning Matrigel Matrix Basement Membrane Growth Factor Reduced)-coated dishes with mTESR1 media (StemCell Technologies) and propagated using Dispase (StemCell Technologies).
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bioRxiv - Genomics 2023Quote: Unedited and WDR90 knock-out iPSC clones were transitioned into feeder-free condition in mTeSR™ Plus medium (STEMCELL Technologies) in a matrigel-coated 6 well plate at a density of 5x105 cells/well with 5 µM ROCK Inhibitor ...
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bioRxiv - Genomics 2023Quote: ... The iPSCs were transitioned into feeder-free conditions inhouse and maintained as feeder-free cultures in mTeSR Plus basal medium supplemented with mTeSR Plus 5X supplement (StemCell Technologies). The cultures were grown in multiwell plates coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Genetics 2023Quote: ... and fed every other day with stem cell media: mTeSR Plus with supplement (STEMCELL Technologies; cat. no. 100-0276) and 0.5% penicillin-streptomycin (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... coated plates in mTeSR1 media (STEMCELL Technologies, #85850). The medium was changed every day and cells were passaged when 70% confluent using ReleSR (STEMCELL Technologies ...
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bioRxiv - Genetics 2023Quote: ... The medium was changed every day and cells were passaged when 70% confluent using ReleSR (STEMCELL Technologies, #100-0484) at a 1:20 ratio ...
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bioRxiv - Biochemistry 2023Quote: ... Doxycycline (Cas No.24390-14-5) from Stem Cell Technologies, and SGK1 inhibitor GS-9007 (Cas No.1426214-51-8 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were collected by centrifugation at 1300 rpm for 5 minutes and 500 K cells distributed into each well of an AggreWell™800 plate (24 well) (Stem Cell Technologies) as recommended by the manufacturer.
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bioRxiv - Developmental Biology 2023Quote: ... After 3 days of growth of naïve PSC were harvested and counted and 55 cells were seeded per microwell (total of 66,000 cells were seeded per 24-well in 1 ml of medium) AggreWell 400 24-well (Stemcell Technologies cat 34415) in N2B27-BSA supplemented with 10 µM ROCKi Y27632 ...
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bioRxiv - Developmental Biology 2023Quote: ... coated plates in mTeSR1 basic medium (Stem Cell Technologies, 85850) supplemented with 1× mTeSR1 supplement (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Human CD34+ cells were purified from fresh BM cells using MicroBead kit (Miltenyi Biotech 130-046-702) and subsequently cryopreserved in CryoStor® CS5 (STEMCELL Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... counted and used for isolation of CD34+ cells using EasySep™ human CD34 positive selection kit II (Stem cell technologies) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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bioRxiv - Developmental Biology 2023Quote: ... 7.5-9.0×104 cells were mixed with 0.5 mL culture medium and seeded into one well of 24-well aggreWell400 culture plate pretreated with anti-adherence rinsing solution (Stem Cell Technologies, 07010) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies, Cologne, Germany) with 50 IE/ml IL-2 ...
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Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... Enzymatic dissociation was performed using Gentle Collagenase/Hyaluronidase (Stemcell Technologies Inc. 07919) in DMEM/F12 supplemented with 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were purified by negative selection using RosetteSepTM Human T cell enrichment cocktail (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... was prepared by pre-coating with anti-adherance solution (07010, STEMCELL Technologies) and centrifuging at 2000g for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 37°C and 5% CO2 in normoxia and in pre-equilibrated human IVC1 supplemented with 50ng/mL Insulin Growth Factor-1 (IGF1) (78078, STEMCELL Technologies) under mineral oil for 1-4h to allow for recovery ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were negatively purified with EasySepTM beads (human T-cell isolation kit, STEMCELL Technologies, Vancouver, Canada). T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%) ...
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: ... according to the protocol with SepMate - 15 tubes (cat. no. 85415, StemCell Technologies) and Lymphoprep (cat ...
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: Samples were depleted of cells of an immune origin with the RosetteSep CD45 depletion kit (depletes with antibodies for CD45, CD66, and Glycophorin A, cat. no. 15122, Stem Cell Technologies, Cambridge, MA USA) according to the protocol with SepMate - 15 tubes (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD34+ cells were purified from PBMNCs by the magnetic separation method (EasySep, Stem Cell Technologies) and were cryopreserved ...
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bioRxiv - Cancer Biology 2023Quote: ... The cryopreserved CD34+ cells of the CML patients and the normal donor were thawed and cultured in CD34+ cell expansion medium constituting StemSpan™ SFEM II medium (Stem Cell Technologies) supplemented with CD34+ Expansion Supplement and UM729 (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... endoderm and mesoderm lineages was carried out using STEMdiff tri-lineage differentiation Kit (Stem Cell Technologies) as per the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2023Quote: ... 7,000 hematopoietic progenitors generated from iPSCs were plated onto MethoCult GF H4636 medium (Stem Cell Technologies). The number of colonies in each experiment was scored on day 14 following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Individual colonies were hand-picked between day 21 and day 32 and were passaged and maintained in mTeSR Plus medium (StemCell Technologies) supplemented with 10µM IM (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were washed with mTeSR Plus (Stem Cell Technologies) containing Revitacell (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... in TeSR-E8 medium (Stem Cell Technologies, Vancouver, Canada) supplemented with 10% knockout serum replacement (Thermo Fisher) ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies). Cells were incubated at 37°C with 5% CO2 ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... medium was changed to SFEM II containing 10ng/mL recombinant human granulocyte colony stimulating factor (StemCell Technologies) and maintained in this medium until day 20 ...