Labshake search
Citations for Stemcell Technologies :
2101 - 2150 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... hPSCs were maintained in feeder-free conditions using mTeSR plus medium (STEMCELL Technologies) on Matrigel (BD Biosciences) ...
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bioRxiv - Developmental Biology 2023Quote: ... according to manufacturer recommendations (StemCell Technologies, Canada, #05872).
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bioRxiv - Developmental Biology 2023Quote: ... The single-cell suspension was passed through a 70-μm reversible strainer (STEMCELL Technologies) and pelleted via centrifugation at 500 g for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... AggreWell™ 400 24-well Plates (STEMCELL Technologies, 34415) were prepared following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... mTeSR media was replaced with RSeTTM Feeder-Free Medium (Stem Cell Technologies, 05975), and the cells were cultured at 37°C in 5% O2 ...
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bioRxiv - Immunology 2023Quote: ... using an EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Separation was carried out with a RoboSep-S according to the manufacturer’s instructions (all Stemcell Technologies).
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bioRxiv - Microbiology 2023Quote: ... After two passages using Accutase (STEM CELL Technologies), cells were verified to be mycoplasma free ...
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bioRxiv - Bioengineering 2023Quote: ... anti CD34-AlexaFluor488 (#60013AD, Stemcell technologies, 1:100).
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bioRxiv - Cell Biology 2023Quote: ... All human CD34+ HSPCs were cultured in StemSpan SFEM II medium (STEMCELL Technologies, Cambridge, MA, USA) supplemented with stem cell factor (SCF ...
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bioRxiv - Neuroscience 2023Quote: ... motor neurons were cultured in Imaging Optimized BrainPhys medium (Stemcell Technologies, 05796) with SM1 NeuroCult supplement (Stemcell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... or ReLeSR (Stemcell Technologies, 05872). The Y-27632 treatment was maintained until the next media change.
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bioRxiv - Neuroscience 2023Quote: ... with SM1 NeuroCult supplement (Stemcell Technologies, 05711), 200 nM L-ascorbic acid (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC lines were maintained with daily media changes in mTeSR1 or mTeSR-Plus medium (Stem cell Technologies, 85870 or 05825) as adherent cultures on plates coated with Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... buffy coat-PBS-citrate solution was added in portions of 25 ml to tubes filled with 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany). Tubes were centrifuged (20 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: Day 5-18 differentiated cells were dissociated with Accutase for 10 min and CD34+ population was purified by magnetic-activated cell sorting (MACS) with an EasySep Magnet kit (STEMCELL Technologies) using a CD34 antibody according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... three million iPSCs were seeded into each well of an AggreWell-800 plate (Stem Cell Technology #34815), resulting in ten thousand cells per microwell ...
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bioRxiv - Genetics 2023Quote: ... biological specimens were individually collected over 37 um filters (Stem Cell Technology #27215) using a dissecting microscope and sterile P1000 pipette tips that were manually cut with sterile disposable scalpels (Fisher Scientific #08-927-5B) ...
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bioRxiv - Genetics 2023Quote: ... we added the following procedures throughout this protocol: (1) we pretreated all plasticware that came into contact with cell aggregates with an anti-adhesion solution (Stem Cell Technology #07010) per manufacturer instructions and (2 ...
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bioRxiv - Genetics 2023Quote: ... all plasticware used to handle the cerebral organoids was pretreated with an anti-adhesion solution (Stem Cell Technology #07010). All fixation steps described in this study were performed using 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2023Quote: ... Matrigel was diluted according to the lot-specific dilution factor recommended by the manufacturer in 25 ml of DMEM/F12 HEPES (Cat No. 36254, STEMCELL Technologies) and set for 1 hour at 37°C before plates were used ...
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bioRxiv - Neuroscience 2023Quote: ... Stem cell pluripotency was validated using the STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies, Cat No. 05230) and human stem cell antibody array from AbCam (Cat No ...
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bioRxiv - Neuroscience 2023Quote: ... Daily media changes were performed for seven days followed by passaging of progenitor cells using Accutase (Cat No. 07920, STEMCELL Technologies), which was repeated for one additional passage (two passages total) ...
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bioRxiv - Neuroscience 2023Quote: ... Stem cell culture was done using a commercially available media kit (mTeSR Plus; Cat No. 100-0276, STEM CELL Technologies) following the manufacturer’s published protocol [32] ...
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bioRxiv - Neuroscience 2023Quote: Astrocyte precursor cells were created from the neural progenitor cells described above using the STEMdiff Astrocyte Differentiation media kit from STEMCELL Technologies (Cat No. 100-0013) following their protocol for Matrigel-coated 6-well plates [30] ...
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bioRxiv - Developmental Biology 2023Quote: ... 10µM ROCK inhibitor Y-27632 (72304, STEMCELL Technologies) was added ...
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bioRxiv - Developmental Biology 2023Quote: ... media was switched to ‘ACL’ (100ng/ml Activin-A, Qk001, QKINE, 3μM CHIR99021, 72052, STEMCELL Technologies, and 10ng/ml human LIF) for hypoblast induction or ‘PA’ (1μM PD0325901 and 1μM A83-01 ...
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bioRxiv - Cancer Biology 2023Quote: ... human iPSCs grown on a Matrigel-coated surface in mTeSR1 (STEMCELL Technologies) were dissociated into single cells with Accutase (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... PSC genomic stability was assessed by screening with the hPSC Genetic Analysis Kit (StemCell Technologies; #07550) which did not indicate the presence of any genomic abnormalities ...
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bioRxiv - Developmental Biology 2023Quote: ... ALDH activity (detected using Aldefluor (Stem Cell Technologies)) and a monoclonal antibody to integrin alpha 6 (Anti-Human/Mouse-CD49f-dFlour450 (Ebioscience ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were cultured in TeSR-E6 medium (STEMCELL Technologies) with 10 μM CHIR99021 (StemRD) ...
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bioRxiv - Immunology 2023Quote: ... coated plates in mTeSR or mTeSR Plus (STEMCELL Technologies). Cell passaging was performed with Accutase (Innovative Cell Technologies) ...
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bioRxiv - Immunology 2023Quote: ... For Il-2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection (StemCell Technologies, Vancouver, BC, Canada). Isolated naïve CD8+ cells (1 x 106 cells/mL ...
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bioRxiv - Genomics 2023Quote: ... Neural rosettes were harvested by treating 14-day-old EBs with STEMdiff Neural Rosette Selection Reagent (Stem Cell Technologies) for 60 minutes at 37°C and were then plated onto polyornithine/laminin- coated plates and cultured with NPC medium containing DMEM/F-12 ...
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bioRxiv - Immunology 2023Quote: Frozen cryovials of 100 million PBMCs (STEMCELL Technologies, Vancouver, BC, Canada) were sorted by FACS following the method described above for three donors ...
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bioRxiv - Immunology 2023Quote: ... using 50 ml SepMate tubes (all from Stemcell technologies). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... All iPSCs were maintained on Matrigel™ (Corning)-coated plates under feeder-free culture condition with mTSER1™ (StemCell Technology) supplemented with 1% penicillin/streptomycin (P/S ...
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bioRxiv - Bioengineering 2023Quote: ... we transferred the organoids to neuronal maturation media containing BrainPhys Neuronal Medium (Stem Cell Technologies # 05790), 1X N-2 Supplement ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×103 cells were resuspended in 1 ml of complete MammoCultTM Basal Medium (Stemcell Tech) and seeded in ultralow attachment plates ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... PBMCs were isolated using Lymphoprep(StemCell Technologies) per manufacturer instructions.
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bioRxiv - Immunology 2023Quote: ... cells were collected from the supernatant and detached from the plate using ACCUTASE (STEMCELL Technologies) for assessment by flow cytometry.
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bioRxiv - Immunology 2023Quote: ... 2 million CD8 T cells (Stemcell Technologies, Cambridge, MA) from three donors were cultured for 48 hours in AIM V Medium (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... containing 500 cells were plated in a well of a SmartDish (StemCell Technologies) in duplicates ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 10 μM Rho-kinase inhibitor (RockI; Y-27632 STEMCELL Technologies, cat. 72304). iPSCs were seeded at a density of 6×104 cells/cm2 and expanded for 3 days to achieve 80% confluency prior to differentiation ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies). Media was replaced with RPMI 1640 medium ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured on recombinant human VTN (rhVTN) coated 60mm plates in StemFlex media (Stem Cell Technologies, cat. A3349401) and passaged using CTS EDTA Versene Solution (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in MethoCult (M3434; #03434, Stemcell technologies) supplemented with doxycycline (1 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated using negative selection (Stemcell Technologies). The NK cells were then suspended in IMDM (Gibco ...