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Citations for Stemcell Technologies :
1501 - 1550 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... isolated from fresh blood samples using a RosetteSep Human NK Cell Enrichment Cocktail (StemCell Technologies), were infused into each lane ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: Neutrophils from healthy donors were isolated with EasySepTM Direct Human Neutrophil Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... in mTeSR+ (Stemcell Technologies, 05825). The medium was changed every 2 days ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies, Vancouver, BC) and were activated with phytohemagglutinin-L (10 µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... 20 ng/ml EGF (STEMCELL Technologies), and 2% B27 supplements (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 20 ng/ml FGF (STEMCELL Technologies), 20 ng/ml EGF (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... CD4 and CD8 T cells were isolated separately using EasySep Human CD4+ or CD8+ Enrichment Kits (Stem Cell Technologies). Isolated T cells were immediately activated with DynaBeads Human T-Activator CD3/CD28 (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... followed by depletion of non-NK lineages using immuno-magnetic beads (EasySep human NK cell enrichment kit, Stemcell Technologies). Samples were sequenced in dedicated flow cells on a HiSeq 2500 (Illumina ...
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bioRxiv - Immunology 2024Quote: NK-92 cells were cultured in Myelocult H5100 (StemCell Technologies) supplemented with 100U/mL IL-2 (Peprotech) ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25+ T cells were isolated from splenocytes of DR3 and DR3.IL17A-/- transgenic mice using EasySep™ Mouse CD4+CD25+ regulatory T cell isolation kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... CD34+ HPCs were differentiated using a commercial feeder-free hematopoietic differentiation kit (STEMdiff Hematopoietic Kit, Stem Cell Technologies). HEK293 and adult normal human dermal fibroblasts (NHDF ...
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bioRxiv - Microbiology 2024Quote: ... The media in both chambers was then replaced with complete Pneumacult-ALI medium (StemCell Technology, Cat. 05001) for an additional day ...
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bioRxiv - Immunology 2024Quote: CD4+CD25+ T cells isolated from the spleens of DR3 and DR3.IL17A-/- transgenic mice using the EasySep™ Mouse CD4+CD25+ regulatory T Cell Isolation Kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ or CD8+ T cells were isolated from mouse spleen and lymph nodes by using a mouse naïve CD4+ or CD8+ T cell isolation kit (STEMCELL Technologies). Purified naïve CD4+ or CD8+ T cells were labeled with CellTrace Violet (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated using Lymphoprep density gradient centrifugation (STEMCELL Technologies), and negative magnetic selection was then used to purify CD4+ T cells (CD4 T cell isolation Kit ...
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bioRxiv - Immunology 2024Quote: ... the pooled blood was carefully layered over 5 mL of Ficoll-Paque PREMIUM 1.084 (Cytiva, Uppsala, Sweden) in 15 mL SepMate-50 tubes (STEM Cell Technology, Cambridge, MA). PBMCs were collected from the interphase layer after centrifugation at 1200xg for 30 min.
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bioRxiv - Immunology 2024Quote: A minimum of 5×104 cells were kept for mononuclear cell lysis from PBMC and SFMC samples before CD4+ T cells enrichment via magnetic negative selection (EasySep™, StemCell Technologies). Tregs were then sorted from CD4+ T cells on live (FVD- ...
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bioRxiv - Immunology 2024Quote: ... and polarized following the manufacturer’s protocol for Immunocult Mouse Th1 or Th2 differentiation supplements (STEMCELL Technologies). For Th17 polarization ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested at 72 hours and Annexin V+ dead/dying cells were depleted using immunomagnetic negative selection (STEMCELL technologies). Isotopomeric Spectral Analysis (ISA ...
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bioRxiv - Immunology 2024Quote: ... Dead or dying cells were depleted using immunomagnetic negative selection (STEMCELL technologies) and RNA was extracted using Trizol and Qiagen RNeasy Kit ...
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bioRxiv - Immunology 2024Quote: ... Human whole-blood samples were diluted with 2% FBS in PBS prior to gradient centrifugation on Lymphoprep (StemCell Technologies). A percentage of the freshly isolated PBMCs was labelled with anti-human CD3 ...
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bioRxiv - Immunology 2024Quote: Monocytes were isolated from healthy human donor buffy coat (Department of Transfusion & Tissue Medicine of the Brno University Hospital) using RosetteSepTM Human Monocyte Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and exposed to the RHO/ROCK pathway inhibitor (10 μM, StemCell Technologies) for 24 hours in incubator at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... DF19-9-7T (61)) were cultured in Matrigel-coated tissue culture dishes and maintained in mTeSR Plus medium (StemCell Technologies) in incubator at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... and Murine IntestiCult (Stemcell Technologies) at a concentration of 60 crypts per 10 µl and seeded into a 96-well plate ...
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bioRxiv - Immunology 2024Quote: ... or SepMate tubes (Stem Cell Technologies) using the manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... The tissue segments were incubated at 37°C with Gentle Cell Dissociation Reagent (Stemcell Technologies) for 5 minutes with shaking at 100 rpm ...
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bioRxiv - Immunology 2024Quote: ... Red blood cells (RBCs) were lysed in BM samples using 0.8% ammonium chloride solution (Stem Cell Technologies). Samples were then directly used for downstream applications ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions harvested from cell culture or isolated from spleen or mediastinal lymph nodes were incubated with 10% Rat serum (STEMCELL Technologies), Fixable Viability Dye (ebioscience ...
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bioRxiv - Immunology 2024Quote: ... Dead or dying cells were depleted using immunomagnetic negative selection (STEMCELL technologies). RNA was isolated using a phenol-chloroform extraction and cDNA was generated using Bio-Rad iScript cDNA synthesis kit ...
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bioRxiv - Immunology 2024Quote: PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 100,000-200,000 CD4+ T cells per well were plated in 96-well V-bottom plates and rested for 1.5 hours at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Single-cell suspensions were stained with 7-AAD (STEMCELL Technologies), and viable cells sorted using BD Aria ...
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bioRxiv - Immunology 2024Quote: ... cells were purified from blood using an EasySep Human Neutrophil Isolation Kit (Stemcell Technologies, Vancouver, Canada) according to manufacturer’s directions ...
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bioRxiv - Immunology 2024Quote: ... and 5 U/ml dispase (#07913 STEMCELL Technologies) with mild agitation for 60 min in a water bath at 37°C ...
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bioRxiv - Immunology 2024Quote: ... ECs cultured on Matrigel-coated cell culture plates were aspirated of residual Endothelial Induction Media (StemCell Technologies) and incubated for 5 minutes at 37°C with 1 mL room temperature Accutase (Gibco ...
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bioRxiv - Immunology 2024Quote: ... after which bulk T cells T cells were enriched using EasySep Human T Cell Enrichment Kits (Stem Cell Technologies), then maintained in RPMI-1640 (ATCC ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were washed in PBS and resuspended in PBS containing 2%FBS and 2 mM EDTA before negative selection using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, #19844A) following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells were purified from mouse spleen and lymph nodes by using a mouse naïve CD4+ T cell isolation kit (STEMCELL Technologies) and polarized following the manufacturer’s protocol for Immunocult Mouse Th1 or Th2 differentiation supplements (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... from de-identified healthy donors were purified from leukapheresis products purchased from Stem Cell Technologies using EasySep Direct Human PBMC Isolation Kits (Stem Cell Technologies), following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) from de-identified healthy donors were purified from leukapheresis products purchased from Stem Cell Technologies using EasySep Direct Human PBMC Isolation Kits (Stem Cell Technologies) ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... All iPSCs were cultured and expanded in mTeSR™ Plus medium (STEMCELL Tech, Catalog # 100-0276) according to the manual.
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... and one 85% medium exchange with IOM supplemented with NeuroCult™ SM1 Neuronal Supplement (SM1) (STEMCELL Tech, Catalog # 05711) containing 10 μM of AT3375 ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Generated NPCs were either used directly or cryopreserved in STEMdiff™ Neural Progenitor Freezing Medium (STEMCELL Tech, Catalog # 05838) and stored in a liquid nitrogen Dewar until use.
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: NPCs were further differentiated to neuronal precursors using the STEMdiffTM Forebrain Neuron Differentiation Kit (STEMCELL Tech, Catalog # 08600) and then matured to forebrain neurons using the STEMdiffTM Forebrain Maturation Kit (STEMCELL Tech ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... The pluripotency of C43-1260 was tested by in vitro differentiation to the three germ layers using the STEMdiff™ Trilineage Differentiation Kit (STEMCELL Tech, Catalog # 05230). All iPSCs were cultured and expanded in mTeSR™ Plus medium (STEMCELL Tech ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... and then matured to forebrain neurons using the STEMdiffTM Forebrain Maturation Kit (STEMCELL Tech, Catalog # 08605) following the standard protocol outlined in the technical manual.
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bioRxiv - Neuroscience 2024Quote: ... containing 10 µM Y- 27632 (cat#: 72302; StemCell Technologies) and 100 µg/mL of the antibiotic Primocin (cat# ...
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bioRxiv - Neuroscience 2024Quote: ... at a density of 3.4 x 104 cells/cm2 in NeuroCultTM Neuronal Plating Medium (STEMCELL Technologies, 05713) supplemented with 2% NeuroCultTM SM1 neuronal supplement (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were maintained in mTeSR™ Plus media (cat#: 100-0276; StemCell Technologies) and kept at 37°C with 5% CO2 with fresh media change every other day ...
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bioRxiv - Neuroscience 2024Quote: ... half of the plating medium was removed from each well and replenished with the same volume of BrainPhys™ Neuronal Medium (STEMCELL Technologies, 05792) supplemented with 2% NeuroCult™ SM1 Neuronal supplement ...