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Citations for Stemcell Technologies :
701 - 750 of 1262 citations for Glucosamine N acetyl 6 Sulfatase GNS Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... human iPSCs were routinely passaged as clumps onto Matrigel-coated plates with mTeSR+ media (StemCell Technologies #85850) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD4 T cells were purified using the naïve human CD4+ T cell isolation kit (StemCell Technology) and stimulated with immobilized anti-human CD3 (5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were isolated from buffy coats by density gradient centrifugation (Lymphoprep®, Stemcell Technologies Cat# 07811) and cultured overnight in T-cell medium (CTS OpTmizer® ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were negatively purified with EasySepTM beads (human T-cell isolation kit, STEMCELL Technologies, Vancouver, Canada). T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%) ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... medium was changed to SFEM II containing 10ng/mL recombinant human granulocyte colony stimulating factor (StemCell Technologies) and maintained in this medium until day 20 ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated using the EasySep Human CD4+ T cell Isolation Kit (Stemcell Technologies, 17952) and subsequently activated using the T Cell Activation/Expansion Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs were differentiated for 12 days using the STEMdiff™ Hematopoietic Kit (#05310, STEMCELL Technologies, Inc.) [43] ...
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bioRxiv - Immunology 2023Quote: Neutrophils of healthy blood donors were isolated using EasySep™ Direct Human Neutrophil Isolation Kit (Stemcell Technologies). Neutrophils were stimulated with immune-complexes as described above and supernatants collected after 4 h ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using an EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2023Quote: ... The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655, STEMCELL Technologies) containing 1% penicillin– streptomycin together with 10 ng/ml rhSCF (#255-SC ...
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bioRxiv - Immunology 2023Quote: ... Cells were enriched for CD8+ population using the EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) and CD8+ cells were used for analysis.
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Immunology 2023Quote: ... and monocytes were isolated from PBMCs by EasySep Human T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada), MACSxpress Whole Blood NK Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Cell Biology 2023Quote: H9 human embryonic stem cells (hESCs; female, WA09, WiCell) were cultured in mTeSR Plus medium (STEMCELL Technologies) with 1% Penicillin-Streptomycin in 8 mg/mL Matrigel-coated (Corning ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (25 µL/mL) (Stemcell Technologies, Cat.#10990) and cultured for 1.5 days at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: The ALDH enzyme activity in human melanoma cells was measured using the Aldefluor assay kit (StemCell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Primary CD8+ T cells were isolated using EasySep Human CD8+ T Cell Enrichment kits (STEMCELL Technologies, 19053) and cultured in RPMI-1640 (ATCC ...
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bioRxiv - Cell Biology 2024Quote: ... and neutrophils were isolated from blood using the EasySepTM direct human neutrophils isolation kit (Stemcell Technologies, 19666). They were then centrifuged at 400 x g for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The resuspended vesicles were layered onto a 6-18% Optiprep™ 11-step discontinuous velocity gradient (Stemcell Technologies, Vancouver, Canada), and centrifuged for 90 min at 176,000 × g in a SW40Ti rotor as previously described (48 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated on 5% laminin at 50,000 cells per well of 6 well-plate and incubated in full growth medium: PneumaCult-Ex medium (Stemcell Tech), glutamax supplement (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were counted and 2 million were cultured for 6 days in hematopoietic expansion media -Stemspan H3000(Stemcell Technologies), with the addition of EPO (R&D) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in 2mL per well T0 media and deposited into AggreWell 400 6-Well plates (StemCell Technologies 34425) prepared with Aggrewell Rinsing Solution (StemCell Technologies 07010 ...
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bioRxiv - Immunology 2022Quote: ... CD19+ cells were purified from C57BL/6 wild-type mice using the EasySep magnetic separation system (Stemcell Technologies, Vancouver, Canada) and were activated with lipopolysaccharide (10 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Genomics 2020Quote: NSTcPA buffer (1 mL) was pipetted into one well of a 6-well plate (Stem Cell Technologies, cat. no. 38015) on ice ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC lines were seeded on pre-treated (0.167 mg/mL Matrigel) 6-well plates and cultured in TeSR-E8 iPSC culture medium (StemCell Technologies) according to the manufacture’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were maintained in NbM for 6 days after which they were passaged with 1 U/mL Dispase (StemCell Technologies) and cultured in NbM supplemented with 0.1 µM Retinoic Acid (RA ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... APCs were seeded onto Matrigel coated 6 well plates at 200,000 cells per well with STEMdiffTM Astrocyte Maturation medium (STEMCELL Technologies). After 2 weeks ...
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bioRxiv - Neuroscience 2022Quote: iPSCs were cultured in feeder-free conditions on Matrigel-coated (0.08 mg/well) 6-well tissue culture plates in mTesR1 media (StemCell Technologies) at 37 °C and 5 % CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were plated on 6-well plates coated with geltrex (Life Technology) and cultured in TeSR-E8 medium (Stemcell Technology). For hiPSC-CMs differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... sorted 250 cells/well were plated in a 6 well plate of MegaCult C Medium plus Lipids (Stem Cell Technologies) with rhEPO (recombinant human erythropoietin) ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2023Quote: ... was pipetted into the cuvette and incubated for 10 minutes at 37°C and then transferred to a fresh well of a Matrigel coated 6-well plate with 2ml of mTeSR1 supplemented with ROCK-inhibitor (10μM, StemCell Technologies). Single GFP-positive cells were sorted in a 96-well plate with mTeSR plus CloneR (StemCell Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... seeded in PLO/Lm coated 6-well plates (EBs produced in one well of AggreWell™ 400 24-well plate were usually seeded into one well of a 6-well plate) and maintained in AAN2 medium (0.5% N2 Supplement (ref.07152, Stemcell Technologies), 200 mM AA-ascorbic acid (ref.12084947 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 or 400 double-positive cells (GFP+ / mCherry+) were plated into a matrigel or laminin-coated 6 cm petri dish in mTeSR supplemented with 1X CloneR (Stemcell technologies). Individual colonies were manually picked into 96 wells approximately 10 days after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... The media was changed daily and the iPSC cultures were split into 1:6-1:10 every 5 days using ReLeSR (Stemcell Technologies).
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bioRxiv - Cell Biology 2021Quote: ... H9-hESC SOX10::GFP reporter and H20961 iPSC were passaged ∼1:6 every 4-5 days by incubating them in ReLeSR (StemCell technologies) for 1 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Then at day 10 ...
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bioRxiv - Genomics 2021Quote: ... on Costar non-treated 6 well polystyrene plates (Catalog Number: 3736; Lot Number: 30417038) coated with Vitronectin XF (Stem Cell Technologies; Catalog Number ...
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bioRxiv - Microbiology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Day 10 organoids were transferred to a rocker to elicit the circulation of nutrients and prevent organoids from sticking to the dish ...
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bioRxiv - Immunology 2022Quote: ... diluted in MethoCult GF-M3434 medium and plated at 20,000 cells/35 mm well in a 6-well plate of low-adherence culture-ware (STEMCELL Technologies, Inc.), in triplicate ...
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bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...