Labshake search
Citations for Stemcell Technologies :
451 - 500 of 648 citations for Mycoplasma Agar 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... were isolated from E13.5 pregnant CD-1 mice and cultured on poly-D-lysine (20 μg/mL) and laminin (10 μg/mL)-coated plates in NeuroCult Basal Medium containing NeuroCult Proliferation Supplement (StemCell Technologies) and 20 ng/mL EGF ...
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bioRxiv - Cell Biology 2021Quote: ... iPSCs were seeded at 9 × 103 cells per well onto 96-well ultralow attachment plates for embryoid body (EB) formation and grown in EB formation media (StemCell Technologies). At day 5 ...
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bioRxiv - Cell Biology 2021Quote: ... the induction of neural epithelium was initiated by moving the EBs into an ultralow attachment 24-well plate where they were then fed with induction media (StemCell Technologies). On day 7 ...
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bioRxiv - Cell Biology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Then at day 10 ...
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bioRxiv - Microbiology 2021Quote: ... the hiPSCs were allowed to self-aggregate in 96-well plates for five days using Neural induction medium (Stem cell technologies) as described earlier (Gabriel et al. ...
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bioRxiv - Genomics 2021Quote: ... on Costar non-treated 6 well polystyrene plates (Catalog Number: 3736; Lot Number: 30417038) coated with Vitronectin XF (Stem Cell Technologies; Catalog Number ...
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bioRxiv - Microbiology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Day 10 organoids were transferred to a rocker to elicit the circulation of nutrients and prevent organoids from sticking to the dish ...
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bioRxiv - Microbiology 2021Quote: ... iPSCs were plated at 9,000 cells per well onto 96-well ultralow attachment plates for embryoid body (EB) formation and grown in EB formation media (StemCell Technologies). At day 5 ...
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bioRxiv - Microbiology 2021Quote: ... the induction of neural epithelium was initiated by moving the EBs into an ultralow attachment 24-well plate and feeding with induction media (StemCell Technologies). On day 7 ...
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bioRxiv - Neuroscience 2021Quote: ... filled with cutting solution before transferring to individual wells in a 12 well plate filled with BrainPhys neuronal medium (StemCell Technologies) with the addition of the following supplements 2% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged and dissociated as described above for EB generation into single cells and cultured on Matrigel-coated plates with the STEMdiff Trilineage differentiation kit (used according to the manufacturer’s instructions, Stemcell Technologies, 05230). Cells were harvested at the indicated day for gene expression analysis by qPCR.
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bioRxiv - Cancer Biology 2021Quote: ... and were plated in a concentration of 9,000 cells/well into an ultra-low-binding 96-well plate in mTeSR™1 medium (Stemcell technologies) containing Rho-associated protein kinase (ROCK ...
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bioRxiv - Cancer Biology 2022Quote: ... were directly sorted into a 96-well tissue culture plate containing 100 μL of differentiation media: StemSpan (Catalog #09650, StemCell Technologies), 1% Penicillin+Streptomycin ...
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bioRxiv - Bioengineering 2022Quote: The colony-forming unit (CFU) assay was performed by seeding 500 cells in six-well plates containing MethoCult Enriched (StemCell Technologies) after 4 days of editing ...
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bioRxiv - Biochemistry 2022Quote: ... NPC cells were cultured on a poly-l-ornithine- and laminin-coated six-well plate (Falcon) at 37°C in neural expansion media (STEMCELL Technologies, 70% DMEM ...
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bioRxiv - Immunology 2022Quote: ... diluted in MethoCult GF-M3434 medium and plated at 20,000 cells/35 mm well in a 6-well plate of low-adherence culture-ware (STEMCELL Technologies, Inc.), in triplicate ...
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bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...
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bioRxiv - Developmental Biology 2022Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). Cells were cryopreserved in mFreSR.
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bioRxiv - Developmental Biology 2022Quote: ... Matrigel-coated wells of a 24-well plate with UPC medium supplemented with 10 µM Y-27632 ROCK inhibitor (STEMCELL Technologies) and incubated at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were dissociated into a single cell suspension with TrypLE and replated on matrigel-coated plates at a density of 40,000 cells/cm2 in mTesR medium (Stem Cell Technologies). Cells were allowed to propagate with a daily change of medium for 2 days ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
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bioRxiv - Neuroscience 2022Quote: ... the dissociated cells were transferred to individual low-adherence V-bottom 96-well plates (S-Bio Prime) in 150μl TeSR5/6 media (StemCell Technologies®) with 50μM Rock inhibitor Y-27632 (Tocris BioScience) ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in new 6-well plate using mTeSR™ plus medium supplemented with 10 μM Y-27,632 Rock inhibitor (StemCell Technologies). Pluripotency of all lines was established by analyzing the expressions of canonical stemness genes (SOX2 ...
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bioRxiv - Developmental Biology 2022Quote: ... For HLC differentiation cells were seeded onto Matrigel™ coated 24-well tissue culture plates at 130,000 cells/ cm2 in mTeSR™ Plus containing 10 μM ROCK inhibitor (STEMCELL Technologies). After 24 hours the differentiation protocol was commenced using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... AP positive colonies were then manually picked and seeded for further expansion onto Matrigel-coated wells of 24-well plates with mTeSR-1 (STEMCELL Technologies) maintenance medium with 10 µM Y-27632 ROCK inhibitor ...
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bioRxiv - Genetics 2022Quote: ... and harvested and replated on day 6 on matrix-coated tissue culture plates and cultivated for 7 days in STEMdiffTM Neural Induction Medium (Stemcell Technologies) with daily medium changes ...
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bioRxiv - Neuroscience 2021Quote: ... were generated from confluent iPSC cultures by dissociating them to single cells and seeding them at a density of ∼4 × 106 cells per well of Aggrewell 800 6-well plates (StemCell Technologies), equating to ∼2200 cells per EB ...
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bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...
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bioRxiv - Developmental Biology 2020Quote: ... Both cell lines were routinely cultured on six-well plates coated with growth factor reduced Matrigel in mTeSR media (STEMCELL Technologies) with daily media replacement ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary patient material was aliquoted into 24- or 48-well plates in 200μl StemSpan™ Serum-Free Expansion Medium (SFEM) (STEMCELL Technologies) supplemented with 20% FCS and the following cytokines ...
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bioRxiv - Evolutionary Biology 2020Quote: Cerebral organoids with telencephalic identity from human and ape cells were generated as previously (Lancaster et al. 2017) with the following modification: Aggrewell 800 plates (StemCell Technologies) containing 300 microwells ...
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bioRxiv - Cell Biology 2021Quote: ... 80,000 cells per well were seeded into a six well plate and differentiated according to the published protocol in organoid culture medium composed of STEMdiff APEL2 medium (STEMCELL Technologies), supplemented with 1% antibiotic-antimycotic and 1% protein-free hybridoma medium II (PFHM 2 ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleofected cells were transferred to a new matrigel-coated well in a 6-well plate in 4mL of E8 medium containing 10μM ROCK inhibitor Y-27632 (StemCell Technologies #72307). Medium was replaced the following day with fresh E8 ...
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bioRxiv - Genomics 2021Quote: ... 20 μl of Matrigel-Crypt-Mix per well were seeded in a 48-well plate and 250 μl of IntestiCult Organoid Growth Medium (06005; STEMCELL Technologies) with Primocin (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... iPSCs obtained from EPCs were maintained in mTeSR1 media on Matrigel-coated polystyrene tissue culture plates and passaged with ReLeSR (STEMCELL Technologies) every 3-5 days ...
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bioRxiv - Developmental Biology 2021Quote: HESCs (H7-WA07) and genome edited H7 derivatives were maintained on Matrigel-coated plates in Essential 8 media (TeSR-E8, Stemcell Technologies)(25) ...
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bioRxiv - Cell Biology 2020Quote: ... H9 ESC (hESC) and hiPSC lines were grown in VTN coated plates in Essential-8 medium (TeSR-E8, Stem Cell Technologies), and dissociated with gentle cell dissociation reagent (0.5mM EDTA in PBS ...
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bioRxiv - Microbiology 2023Quote: ... A final boost in PBS was administered and 4 days later splenocytes were fused with Sp2/10 Ag14 cells (ATCC CRL-1581) and cultured overnight prior to concurrent cloning and HAT selection on methylcellulose plates (ClonaCell-HY, StemCell Technologies). After 14 days of incubation at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and the cells were then replated on matrigel-coated plates in mTesR plus media at a density of 40,000 cells/cm2 (Stem Cell Technologies). Cells were allowed to proliferate for two days with a daily media change ...
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bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Current protocol is optimized for 16 bEPSCs and 16 bTSCs per well in a ∼1200 well Aggrewell 400 microwell culture plate (Stemcell technologies) for a total of 19,200 of each cell types per well ...
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bioRxiv - Developmental Biology 2023Quote: ... they were collected and moved to a new 96-well plate containing 150 µl of induction media (Cerebral organoid kit, cat. n. 08570, StemCell Technologies) per well ...
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bioRxiv - Neuroscience 2023Quote: ... Six-well plates coated for at least 2h with 10µg/mL of Cell Adhere Laminin 521 (Stem Cell Technology, 200-0117) diluted in DPBS containing calcium and magnesium (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs were digested with Accutase and then differentiated onto PLO/laminin coated plates/coverslips with final differentiation media which is BrainPhys media (STEMCELL Technologies) supplemented with Glutamax (Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Neural progenitor cells were then dissociated and replated on poly-l-ornithine/fibronectin/laminin-coated plates and maintained in BrainPhys Basal medium (StemCell Technologies) containing B-27 supplement ...
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bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270 ...
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bioRxiv - Physiology 2023Quote: ... Cells were then plated between 450,000 - 750,000 cells per well of a Matrigel coated 12-well tissue culture plate in mTeSR1 PLUS™ (Stem Cell Technologies) supplemented with 10 µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were cultured for 72 hours in 96-well plates (2X105-5X105 cells per well) containing StemPro (Stem Cell Technologies) media supplemented with human IL-6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...