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Citations for Primerdesign :
1 - 40 of 40 citations for rno mir 194 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... a Primer Design Real-Time PCR Internal Control Kit (PrimerDesign, Southampton, United Kingdom) was used ...
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bioRxiv - Developmental Biology 2022Quote: ... The reaction was set up as follows: 1x of PrecisionFastTM qRT- PCR Master Mix with Low ROX (Primerdesign Ltd., UK), 200 nM of each primer ...
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bioRxiv - Plant Biology 2019Quote: ... Transcript levels were measured by reverse transcription quantitative PCR (RT-qPCR) using Sybr Green master mix (Primerdesign Ltd.) and a StepOnePlusTM Real Time PCR System (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Primers were from PrimerDesign, Eurofins Genomics or Thermo Fisher Scientific (see Supplementary Table S4) ...
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bioRxiv - Bioengineering 2019Quote: ... Gene expression was measured directly from 5 ng RNA using a one-step RT PCR kit with SYBR dye (PrimerDesign). qPCR was run on the BioRad CFX96 platform ...
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bioRxiv - Genetics 2019Quote: ... Prevalidated primers (PrimerDesign Ltd UK) were used for these experiments unless otherwise stated ...
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bioRxiv - Neuroscience 2020Quote: ... Primers (S3 Table) were obtained from PrimerDesign Ltd ...
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bioRxiv - Cell Biology 2021Quote: ... Custom designed and validated primers (PrimerDesign Ltd) were used as follows:
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bioRxiv - Neuroscience 2020Quote: ... GAPDH primers were proprietary and supplied by Primerdesign Ltd ...
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bioRxiv - Immunology 2021Quote: ... Primers (Supplementary Table 3) were purchased from Primerdesign Ltd (Primerdesign Ltd ...
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bioRxiv - Genetics 2021Quote: ... or Precision Reverse Transcription Premix (PrimerDesign, cat. no: RT-premix2). Primers for sequencing cDNA for testing Espn splicing in Espnspdz mice were designed using Primer375 (Supplementary Table 3) ...
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bioRxiv - Systems Biology 2020Quote: ... or Precision Reverse Transcription Premix (PrimerDesign, cat. no: RT-premix2). MicroRNA cDNA was made using the miRCURY LNA RT Kit (QIAGEN ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Primers for target and positive control genes were designed by Primerdesign Ltd and Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... Primer sequences shown in appendix table 1 are copyrighted by PrimerDesign Ltd UK ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was reverse transcribed (RT) by Precision Nanoscript2 (PrimerDesign, UK), following manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Primerdesign primers against MMP8 (JN215643) or ADAMTS9 (JN171532) (Primerdesign, Chandlers Ford, UK) or designed in-house (Supplementary Table 1) ...
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bioRxiv - Biochemistry 2022Quote: ... RT-qPCR reactions for tissue samples were prepared using Precision SYBRgreen Mastermix (Primerdesign, UK), 175 nM forward and reverse primers (Eurofins ...
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bioRxiv - Cell Biology 2019Quote: ... Assays were performed using primers (Supplementary table 2) and a SYBR green master mix (Primerdesign) on a StepOnePlus system (Applied Biosciences) ...
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bioRxiv - Genomics 2020Quote: ... RT-qPCR reactions were performed using PrecisionPLUS-MX-SY Mastermix (containing SYBR Green; Primerdesign Ltd) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription primed by random nonamer primers was conducted using nanoScript 2 Reverse Transcription kit (Primerdesign). The qPCR was performed using SYBR green based PrecisionFAST kit (Primerdesign ...
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bioRxiv - Cell Biology 2021Quote: ... The subsequent reverse transcription was conducted using nanoScript 2 Reverse Transcription kit and random nanomer primers (Primerdesign). The real time PCR was performed on Bio-Rad CFX96 Touch using SYBR-green-based PrecisionFAST with LOW ROX qPCR kit (Primerdesign) ...
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bioRxiv - Microbiology 2022Quote: ... using FAM labeled HCMV primer and probe: Human CMV HHV5 kit for qPCR using a glycoprotein B target (PrimerDesign); and HEX labeled RPP30 copy number assay for ddPCR (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng RNA was used as template for quantitative PCR with Precision OneStep qRT-PCR master mix (PrimerDesign, Southampton, UK). Quantitative PCR was performed with a StepOnePlus Real-Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Twenty-five nanograms of total RNA was used per reaction with PrecisionPLUS OneStep RT-qPCR Master Mix (PrimerDesign, UK) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Data were normalised to the geometric mean of the housekeeping genes (ubiquitin C and glyceraldehyde 3-phosphate dehydrogenase, probe-based duplex primer mix, PrimerDesign) and fold change in gene expression relative to controls was determined using the ΔΔCt method ...
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bioRxiv - Microbiology 2022Quote: ... swine fever or PRRSV was assessed by a multiplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) detection kit (Microplasma 16 s Ribosomal RNA Gene Genesig® Standard kit, [Primerdesign, Camberley ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 µg RNA was used for cDNA synthesis with equal amounts of oligo(dT) and random nonamer primers using the nanoScript2 reverse transcription kit (Primerdesign Ltd., Southampton, UK). Transcript levels were measured by reverse transcription quantitative PCR (RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Relative quantification was derived using 2-ΔCt (ΔCt calculated from the mean of two normalising genes, ATP5B and B2M (PrimerDesign Ltd, primer sequence proprietary). The following primers were used ...
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bioRxiv - Microbiology 2024Quote: Real time PCR (qPCR) was performed with the PrecisionPLUS qPCR Master Mix (Primerdesign, UK) per the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... Gene expression was determined based on real-time quantitative PCR using SYBR green (PP00259, Primerdesign). The data was analyzed with the ΔΔCT method and normalized to the housekeeping gene 36b4 ...
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bioRxiv - Microbiology 2019Quote: ... RNAemia levels were measured by a One-Step qRT-PCR detection kit (Oasig, Primerdesign Ltd., UK) and using DENV RT primer/probe Mix kit (Genesig ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 µl qRT-PCR reactions were performed using Precision PLUS qPCR Master Mix with SYBR green (PrimerDesign) in 384-well white plates on a Roche LightCycler 480 ...
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bioRxiv - Cell Biology 2021Quote: ... The real time PCR was performed on Bio-Rad CFX96 Touch using SYBR-green-based PrecisionFAST with LOW ROX qPCR kit (Primerdesign). The reading was normalized by that of β-tubulin transcript (TUBB) ...
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bioRxiv - Immunology 2021Quote: ... to measure HRV-16 replication in AEC cultures we used the Genesig® Human Rhinovirus Subtype 16 PCR Kit (Primerdesign®).
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bioRxiv - Microbiology 2020Quote: Nucleic acids were used for the detection of tilapia lake virus and nodavirus by quantitative PCR using the commercial kits: Path-TiLV-EASY and Path-Betanodavirus-EASY (Primerdesign, Southampton, UK) in the platform Genesig q16® (Primerdesign ...
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bioRxiv - Immunology 2021Quote: ... To measure SARS-CoV-2 replication in AEC cultures we used the Genesig® Coronavirus Strain 2019-nCoV Advanced PCR Kit (Primerdesign®), with duplicate assays of harvested RNA from each SARS-CoV-2-infected AEC experimental condition ...
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bioRxiv - Physiology 2021Quote: Detection of murine Col1a2 wild-type and mutant alleles was performed using a custom snpsig™ real-time PCR mutation detection/allelic discrimination kit (Primerdesign, Southampton, UK). 10 ng of cDNA was added to 10 μl of PrecisionPLUS mastermix (Primerdesign ...
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bioRxiv - Microbiology 2020Quote: ... Samples were initially examined for SARS-CoV-2 RNA by COVID-19 Genesig® Real-Time PCR assay (Primerdesign Ltd., Chander’s Ford, UK) after RNA extraction with the Magna Pure Compact system (Roche Molecular Systems Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... with triplicate assays of harvested RNA from each SARS-CoV-2-infected AEC donor cell line (Genesig® Coronavirus Strain 2019-nCoV Advanced PCR Kit, Primerdesign®, Southampton, UK). The concentration of RNA harvested from AECs was used to normalize the qPCR data and was measured on a spectrophotometer (Nanodrop®).