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Citations for Primerdesign :
1 - 24 of 24 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Real time PCR (qPCR) was performed with the PrecisionPLUS qPCR Master Mix (Primerdesign, UK) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng RNA was used as template for quantitative PCR with Precision OneStep qRT-PCR master mix (PrimerDesign, Southampton, UK). Quantitative PCR was performed with a StepOnePlus Real-Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 µl qRT-PCR reactions were performed using Precision PLUS qPCR Master Mix with SYBR green (PrimerDesign) in 384-well white plates on a Roche LightCycler 480 ...
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bioRxiv - Plant Biology 2019Quote: ... Transcript levels were measured by reverse transcription quantitative PCR (RT-qPCR) using Sybr Green master mix (Primerdesign Ltd.) and a StepOnePlusTM Real Time PCR System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... The reaction was set up as follows: 1x of PrecisionFastTM qRT- PCR Master Mix with Low ROX (Primerdesign Ltd., UK), 200 nM of each primer ...
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bioRxiv - Genetics 2020Quote: ... Reactions were performed with PrecisionTM 2X qPCR master mix (Primerdesign) in 10 μL volumes using the LightCycler® 480 System (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... qPCR was performed using Precision™ 2X qPCR master mix (Primerdesign) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Assays were performed using primers (Supplementary table 2) and a SYBR green master mix (Primerdesign) on a StepOnePlus system (Applied Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... Twenty-five nanograms of total RNA was used per reaction with PrecisionPLUS OneStep RT-qPCR Master Mix (PrimerDesign, UK) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 20μL reactions were prepared in triplicate in 96-well white plates consisting of 10μL PrecisionPLUS qPCR Master Mix with SYBR green (Primerdesign, UK), 5.2μL nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR reactions were performed using the 2X Precision®FAST qPCR Master Mix (with SYBR-green and low ROX) according to manufacturer’s instructions (PrimerDesign LTD). The following primers were used (5’→3’) ...
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bioRxiv - Microbiology 2019Quote: ... and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Data were normalised to the geometric mean of the housekeeping genes (ubiquitin C and glyceraldehyde 3-phosphate dehydrogenase, probe-based duplex primer mix, PrimerDesign) and fold change in gene expression relative to controls was determined using the ΔΔCt method ...
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bioRxiv - Molecular Biology 2019Quote: ... a Primer Design Real-Time PCR Internal Control Kit (PrimerDesign, Southampton, United Kingdom) was used ...
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bioRxiv - Physiology 2019Quote: ... Gene expression was determined based on real-time quantitative PCR using SYBR green (PP00259, Primerdesign). The data was analyzed with the ΔΔCT method and normalized to the housekeeping gene 36b4 ...
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bioRxiv - Microbiology 2019Quote: ... RNAemia levels were measured by a One-Step qRT-PCR detection kit (Oasig, Primerdesign Ltd., UK) and using DENV RT primer/probe Mix kit (Genesig ...
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bioRxiv - Bioengineering 2019Quote: ... Gene expression was measured directly from 5 ng RNA using a one-step RT PCR kit with SYBR dye (PrimerDesign). qPCR was run on the BioRad CFX96 platform ...
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bioRxiv - Cell Biology 2021Quote: ... The real time PCR was performed on Bio-Rad CFX96 Touch using SYBR-green-based PrecisionFAST with LOW ROX qPCR kit (Primerdesign). The reading was normalized by that of β-tubulin transcript (TUBB) ...
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bioRxiv - Immunology 2021Quote: ... to measure HRV-16 replication in AEC cultures we used the Genesig® Human Rhinovirus Subtype 16 PCR Kit (Primerdesign®).
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bioRxiv - Microbiology 2020Quote: Nucleic acids were used for the detection of tilapia lake virus and nodavirus by quantitative PCR using the commercial kits: Path-TiLV-EASY and Path-Betanodavirus-EASY (Primerdesign, Southampton, UK) in the platform Genesig q16® (Primerdesign ...
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bioRxiv - Immunology 2021Quote: ... To measure SARS-CoV-2 replication in AEC cultures we used the Genesig® Coronavirus Strain 2019-nCoV Advanced PCR Kit (Primerdesign®), with duplicate assays of harvested RNA from each SARS-CoV-2-infected AEC experimental condition ...
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bioRxiv - Physiology 2021Quote: Detection of murine Col1a2 wild-type and mutant alleles was performed using a custom snpsig™ real-time PCR mutation detection/allelic discrimination kit (Primerdesign, Southampton, UK). 10 ng of cDNA was added to 10 μl of PrecisionPLUS mastermix (Primerdesign ...
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bioRxiv - Microbiology 2020Quote: ... Samples were initially examined for SARS-CoV-2 RNA by COVID-19 Genesig® Real-Time PCR assay (Primerdesign Ltd., Chander’s Ford, UK) after RNA extraction with the Magna Pure Compact system (Roche Molecular Systems Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... with triplicate assays of harvested RNA from each SARS-CoV-2-infected AEC donor cell line (Genesig® Coronavirus Strain 2019-nCoV Advanced PCR Kit, Primerdesign®, Southampton, UK). The concentration of RNA harvested from AECs was used to normalize the qPCR data and was measured on a spectrophotometer (Nanodrop®).