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Citations for Macherey-Nagel GmbH :
1 - 50 of 58 citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... RNase-free (Macherey-Nagel). M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). In vitro methylation experiments were performed for 10 min (DNA ...
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bioRxiv - Biochemistry 2023Quote: ... and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). In vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer (New England Biolabs Inc. ...
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bioRxiv - Plant Biology 2022Quote: ... following the manufacturer’s instructions and treated with RNase-free DNase (Macherey-Nagel) to remove trace genomic DNA ...
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bioRxiv - Microbiology 2021Quote: ... and 2,5μL of RNAse (Macherey-Nagel) at 1000 unit and 3,5mg/mL ...
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bioRxiv - Microbiology 2022Quote: ... and 2.5μL of RNAse (Macherey-Nagel) at 1000 unit and 3.5 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... and NucleoMag® DNA/RNA Water kit for water and air sample (Macherey-Nagel, Hoerdt, France), referred to as the “Water” ...
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bioRxiv - Microbiology 2023Quote: ... and 2.5 µl of RNAse (Macherey-Nagel) at 1000 unit and 3.5 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 250 μg/mL of RNase A (Macherey-Nagel) and 0.5 mg/mL of lysing enzymes from Trichoderma harzianum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... sodium deoxycholate 1.5%) containing 200 μg/mL of RNAse A (Macherey-Nagel), and incubated during 15 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then treated with RNase (Macherey-Nagel, Thermo Fisher Scientific), stained with propidium iodide (PI ...
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bioRxiv - Microbiology 2021Quote: ... produced using an endotoxin-free kit (Macherey-Nagel), were co-injected with the I-SceI meganuclease (Grabher et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... pooled viral supernatants were digested with 0 or 400 ng RNase A (Macherey-Nagel, #740505.50) for 4 hours at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... excess egg water was removed and 350 μl lysis buffer added (NucleoSpin, MACHEREY-NAGEL). The larvae were homogenized by repeatedly drawing through a 26-gauge needle into a syringe and expelled ...
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bioRxiv - Bioengineering 2023Quote: ... a total of 6µg of endotoxin free plasmids (Macherey-Nagel, 740424 ...
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bioRxiv - Plant Biology 2023Quote: ... then added 1.2 µL of RNase (MN, Macherey-Nagel, Ref. 740505, 1200 U/mL, 10 mg/mL), and transferred the DNA to a 96-well KingFisher Flex plate (ThermoFischer Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were isolated using an endotoxin-free plasmid midiprep kit (Macherey-Nagel) for QF2 lines and NucleoBond Xtra Midi Endotoxin-Free plasmid kit (Clontech 740420.50 ...
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bioRxiv - Microbiology 2022Quote: ... All DNA used for transfection was prepared LPS-free (Macherey-Nagel, 740410.50).
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bioRxiv - Developmental Biology 2023Quote: ... cells were resuspended in PBS with 20 µg/ml PI and 0.2 mg/mL RNAse A (Macherey-Nagel) and incubated at RT for 30 min before analysis on a FACSCalibur (Beckton Dickinson) ...
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bioRxiv - Genomics 2022Quote: ... Maxiprep was performed with the Nucleobond Xtra endotoxin-free maxiprep kit (Macherey-Nagel).
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bioRxiv - Cancer Biology 2020Quote: ... treatment with 100µg/ml RNase A (37° for 15 minutes) and 50µg/ml proteinase K and purification using the PCR cleanup kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2020Quote: ... Amplified plasmids were isolated using an endotoxin-free plasmid isolation kit (Macherey-Nagel, Germany).
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bioRxiv - Molecular Biology 2023Quote: All plasmids were purified using the Endotoxin-free plasmid DNA purification kit (Macherey-Nagel), the total volume was adjusted to 10% (ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and plasmid DNA was extracted with a Maxiprep endotoxin-free kit (Macherey-Nagel, 740424.10).
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bioRxiv - Molecular Biology 2023Quote: ... and endotoxin-free plasmid DNA was isolated using anion exchange columns (NucleoBond, Macherey-Nagel).
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bioRxiv - Microbiology 2023Quote: ... plasmid Midi Preps were prepared using NucleoBond Xtra Midi Endotoxin-free Kit (Macherey-Nagel).
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bioRxiv - Cancer Biology 2023Quote: ... coli under chloramphenicol selection and purified with the Endotoxin-Free MaxiPrep kit (Macherey-Nagel).
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bioRxiv - Neuroscience 2024Quote: ... and purified using an endotoxin-free midiprep kit (NucleoBond Xtra Midi EF, Macherey-Nagel). Purified plasmid DNA was diluted to a final concentration of 135-150 ng/µL in a solution containing (in mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed cells were washed twice with PBS and stained with 20 µg/mL propidium iodide (PI) containing 100 mg/mL RNAse A (740505, MACHEREY-NAGEL) for 15 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Endotoxin free DNA was prepared using respective kits (Macherey-Nagel GmbH & Co. KG, Düren, Germany), quantified with a NanoDrop Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μL water was added and DNA was purified using Nucleospin Gel and PCR Cleanup (Macherey-Nagel, Cat. No 740609.250). Extracted DNA was PCR-amplified to check the insertion of the 3XFLAG tag ...
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bioRxiv - Microbiology 2019Quote: ... compounds were separated by reverse-phase chromatography using Ultra Performance Liquid chromatography (UPLC IClass, Waters) using a Nucleodur C18ec column (2.0 mm × 150 mm, 5 μm particle size, Macherey-Nagel). Elution was achieved by increasing the acetonitrile/water (milliQ filtrated on 0.22 μm ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted in triplicate for mixed cell suspensions using either NucleoMag® DNA/RNA Water (Macherey-Nagel, Hoerdt, France), i.e. ...
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bioRxiv - Plant Biology 2021Quote: ... The aqueous phase was transferred to a new tube and incubated with 1 μL of RNase A (Macherey-Nagel, 12mg/mL stock) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Eluates were digested with proteinase K and RNAse A and DNA fragments were purified using a PCR purification kit (Macherey-Nagel, #740609).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... pBDNF was purified using the endotoxin-free plasmid purification NucleoBond®Xtra system (Macherey-Nagel, Germany). The final concentration was measured by OD260 using an UV-visible spectrophotometer (NanoDrop™ ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA for cell-free reactions was prepared from overnight bacterial cultures using Maxiprep kits (Macherey-Nagel).
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bioRxiv - Cell Biology 2024Quote: ... Plasmid DNA was isolated via the endotoxin-free NucleoBond Xtra Midi Plus EF kit (Macherey-Nagel). For transfection ...
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bioRxiv - Plant Biology 2021Quote: ... The eluate was diluted with distilled water and passed over a Chromabond C18ec 100 mg solid phase extraction column (Macherey-Nagel). The eluate was evaporated in a vacuum concentrator ...
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bioRxiv - Genetics 2022Quote: ... cells were scraped from plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel). The resulting plasmid DNA was used for yeast transformation.
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bioRxiv - Genetics 2022Quote: ... the cells were scraped from the plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
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bioRxiv - Microbiology 2022Quote: ... The 0.22 µm membrane was used for bacterial DNA isolation using NucleoMag DNA/RNA Water Kit (MACHEREY-NAGEL Inc., Bethlehem, PA) following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... resuspended in sterilized water and filtered under vacuum using a 0.2 µm pore size filter (predried and preweighted; PORAFIL® MV; Macherey-Nagel). The filter was once more dried (overnight 12-24 h in an oven at 60⁰C ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was diluted with 4.5 ml water and subjected to solid-phase extraction on HR-XC (Chromabond, Macherey-Nagel, Düren, Germany) column ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... For solid phase extraction different cartridges (HLB Oasis HLB and WCX, both Waters, Milan, Italy, as well as Chromabond C18, C18 Hydra, and HR-X, all Macherey-Nagel, Düren Germany), were tested ...
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bioRxiv - Plant Biology 2019Quote: ... The cooled samples were filled up with deionized water to a volume of 25 ml and filtered through ashless filter paper (MN640 m, Ø125 mm, manufactured by Macherey-Nagel, Düren, Germany). Absorption was measured at 213.8 nm for Zn and 248.3 nm for Fe with an atomic absorption spectrometer (model 11003 ...
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bioRxiv - Cell Biology 2020Quote: ... were purified using an endonuclease free plasmid extraction kit (NucleoBond Xtra Maxi Plus EF, Macherey-Nagel, cat# 740426.10).