Labshake search
Citations for Macherey-Nagel GmbH :
1 - 50 of 323 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... PCR products were purified by agarose electrophoresis and NucleoSpin kit (Macherey-Nagel). 1µg of purified DNA was used for in-vitro transcription following the manufacturer instructions (HiScribe T7 High Yield RNA Synthesis Kit ...
-
bioRxiv - Molecular Biology 2023Quote: ... purified through agarose gel electrophoresis using NucleoSpin Gel and PCR Cleanup (Macherey-Nagel), eluted in water and concentrated SpeedVac Concentrator SAVANT SPD 121P (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... Correct products were isolated with gel electrophoresis and gel extraction (Macherey-Nagel, F40609.250), and were ligated with T4 DNA ligase (Thermo Fisher ...
-
bioRxiv - Cell Biology 2020Quote: PCR products were analyzed by agarose gel electrophoresis and purified using column purification (Macherey-Nagel).
-
bioRxiv - Cancer Biology 2023Quote: ... PCR products were evaluated by electrophoresis and then further processed with the PCR clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: ... PCR products were checked by agarose gel electrophoresis and purified using Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Cycle sequencing reactions were performed using the BigDye 3.1 chemistry ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected by gel electrophoresis on 2% Agarose/TAE and gel extracted by column purification (Macherey-Nagel). Amplicons were paired-end sequenced with 500 cycles on a MiSeq system (Illumina ...
-
bioRxiv - Immunology 2020Quote: ... amplicons were separated by gel electrophoresis and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Library quantification and quality control was performed using Qubit (QIAGEN ...
-
bioRxiv - Immunology 2020Quote: ... The amplified products were identified by gel electrophoresis and purified using Nucleospin PCR and Gel Clean Up Kit (Macherey-Nagel # 740609.5). Alternatively ...
-
bioRxiv - Bioengineering 2019Quote: ... PCR products were analysed by agarose gel electrophoresis and gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Constructs were transformed into E ...
-
bioRxiv - Plant Biology 2020Quote: ... DNA fragments were purified by agarose gel electrophoresis followed by extraction from an excised gel slice using a NucleoSpin Extract II kit (Macherey-Nagel). Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit ...
-
bioRxiv - Microbiology 2023Quote: ... Amplified libraries were cleaned up after gel electrophoresis (selected size between 200 bp to 500 bp) by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions for high percentage agarose gels.
-
bioRxiv - Systems Biology 2023Quote: ... The PCR products of correct size were purified by agarose gel electrophoresis (NucleoSpin Gel and PCR Clean-up Kit, Macherey-Nagel). The purified linear DNA was used for cloning of pTS040 by Gibson assembly (NEBuilder HiFi DNA Assembly Reaction ...
-
bioRxiv - Cell Biology 2023Quote: ... The amplicons were resolved by agarose electrophoresis and extracted from the gel using NucleoSpin Gel and PCR Clean-up XS (Macherey-Nagel, Düren, Germany). The extracted DNA was then processed into libraries for sequencing using Ion Plus Fragment Library Kit and Ion Xpress Barcode Adapters 1-16 (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... The DNA product was verified by gel electrophoresis and purified with the NuceloSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.50). The concentration of the purified PCR product was adjusted to 5 ng/μl in 15 μl and send to Sanger sequencing by Eurofins Genomics ...
-
Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... Cells were washed with PBS once and RNA isolated from lysed cells Nucleospin RNA kit (#740955.50, Macherey-Nagel) according to manufacturer’s instructions including rDNAse treatment ...
-
bioRxiv - Microbiology 2021Quote: ... or cells (NucleoSpin RNA plus kit, Macherey-Nagel) at different time post-electroporation (4 hpe – 7 dpe) ...
-
bioRxiv - Cell Biology 2022Quote: Cells were lysed with Nucleozol (Macherey-Nagel, 740404) and RNA extractions were conducted according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were collected 5 days after transfection and the cell’s genomic DNA was prepared using NucleoSpin Tissue (MACHEREY-NAGEL & Co. KG).
-
bioRxiv - Cancer Biology 2020Quote: Cells were lysed with Nucleozol™ (Macherey-Nagel, 740404) and RNA extraction was conducted according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: RNA was harvested from the cells (Macherey-Nagel; 740955) and subjected to cDNA synthesis with oligo(dT ...
-
bioRxiv - Cell Biology 2020Quote: ... coli DH10 cells using a BAC prep kit (Macherey-Nagel). P19 Copg1-/- cells were transfected with 1µg BAC DNA using Effectene (QIAGEN) ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA purification from cultured cells and tissue (740955; Macherey-Nagel). Complementary cDNA was synthesised using Maxima first strand cDNA kit from five hundred nanograms of purified total RNA (K1641 ...
-
bioRxiv - Biochemistry 2019Quote: Total RNA was extracted from HEK293T cells using NucleoZOL (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were collected in Nucleoprotect RNA solution (Macherey-Nagel, Dueren, Germany). Bacteria were gated as described above ...
-
bioRxiv - Cell Biology 2023Quote: RNA was extracted from cells using Nucleospin RNA (Macherey-Nagel; 740955) and cDNA was synthesized from 1 μg of total RNA using the M-MLV (Invitrogen ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and purified edited cells using a NucleoSpin Tissue Kit (Macherey-Nagel). The HDRT integration site was PCR-amplified from genomic DNA using Q5 High-Fidelity Polymerase (NEB) ...
-
bioRxiv - Microbiology 2024Quote: ... The cells were lysed with Nucleobond buffer set iii (MACHEREY-NAGEL) and the chromosomal DNA was purified with Sera-Mag magnetic beads (Cytiva) ...
-
bioRxiv - Microbiology 2021Quote: ... half of the cells was lysed in RA1 lysis buffer (Macherey-Nagel) (“on ice”) ...
-
bioRxiv - Microbiology 2020Quote: ... Total DNA was extracted from cell pellets using NucleoSpin Tissue (Macherey-Nagel) kit ...
-
bioRxiv - Genetics 2019Quote: ... coli BL21-Gold cells and purified on Ni-NTA Agarose (Macherey-Nagel). Two guinea pigs were immunized at Bioscience with His-DBN-1(1-132 ...
-
bioRxiv - Developmental Biology 2022Quote: ... RNA was collected from cells using the NucleoSpin RNA Kit (Macherey-Nagel) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: RNA from mESC and HEK293T cells was extracted with NucleoZOL (Macherey-Nagel); from MCF7 cells BIO TRI RNA reagent (Bio-lab ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were then treated with RNase (Macherey-Nagel, Thermo Fisher Scientific), stained with propidium iodide (PI ...
-
bioRxiv - Biophysics 2022Quote: ... coli MC1061 cell line using the Nucleobond Xtra Maxi Kit (Macherey-Nagel). 1.3 μg DNA was used per 106 cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and processed using the NucleoSpin RNA kit (Macherey-Nagel) or the PureLink RNA Mini Kit (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... total RNA was isolated from cell lines using NucleoSpin RNA Kit (Macherey-Nagel) and subjected to Real-Time PCR using the high-capacity cDNA reverse transcription kit (Applied Biosystems ...
-
bioRxiv - Developmental Biology 2021Quote: Total RNA from cultured cells was isolated using NucleoSpin®RNA (Macherey-Nagel), Düren ...
-
bioRxiv - Cell Biology 2022Quote: RNA was isolated from cells using a NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2019Quote: Genomic DNA was isolated from sorted cells using NucleoSpin Blood kits (Macherey-Nagel). PCR was used to amplify gRNA cassettes with Illumina sequencing adapters and indexes as described previously43 ...
-
bioRxiv - Biochemistry 2020Quote: ... coli NM522 cells then extracted using a NucleoBond Xtra Midi kit (Macherey-Nagel). Quantification was performed using a SimpliNano spectrophotometer (Biochrom).
-
bioRxiv - Cell Biology 2020Quote: Total DNA was extracted from cells using NucleoSpin Tissue (Macherey-Nagel, Duren, Germany). The extracted DNA (100 ng ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from cultured cells using Mini spin kit (Macherey-Nagel). RNA extracted from canine fibroblasts was obtained from Prof ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were either fixed with 4% paraformaldehyde or lysed with NucleoZOL (MACHEREY-NAGEL) 17 days post infection ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA of cells was isolated with Nucleo Spin miRNA Kit (Macherey-Nagel), total RNA of melanoma samples was isolated with RNeasy Kit (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: ... cells were plated on sterile filters (0.2 µm pore size, MN615, Macherey-Nagel), placed on BG11 agar plates and incubated under standard conditions ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
-
bioRxiv - Cancer Biology 2019Quote: RNA was extracted from S2 cells using Nucleospin RNA II kit (Macherey-Nagel) and cDNA was synthesized using SensiFAST cDNA Synthesis kit (Bioline ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α cells for maxiprep using the NucleoBond Xtra Maxi kit (Macherey-Nagel). Linear DNA fragments used as expression templates in the cell-free reactions were PCR amplified using primers and templates listed in Sup Table 7 ...
-
bioRxiv - Genomics 2020Quote: ... RNA was extracted from cells using the NucleoSpin RNA mini kit (Macherey-Nagel) with on-column deoxyribonuclease treatment ...