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Citations for Macherey-Nagel GmbH :
1 - 50 of 718 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and purified by affinity column purification (Macherey-Nagel). Reverse transcription was performed using Maxima Reverse Transcriptase (Thermo Fisher) ...
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bioRxiv - Biophysics 2022Quote: ... and the protein was affinity- purified from lysate using Ni-NTA affinity resin (Macherey-Nagel) and elution with imidazole ...
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bioRxiv - Microbiology 2020Quote: ... PomXK13AR15A-His6 was affinity purified with Protino Ni-NTA resin (Macherey-Nagel) from a batch ...
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bioRxiv - Microbiology 2020Quote: ... PomXC-His6 was affinity purified with Protino Ni-NTA resin (Macherey-Nagel) from a batch ...
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bioRxiv - Microbiology 2020Quote: ... PomXN-His6 was affinity purified with Protino Ni-NTA resin (Macherey-Nagel) from a batch ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (DE3) and affinity purified using Protino Ni-NTA Agarose (Macherey-Nagel). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM PMSF and purified by immobilized metal affinity chromatography using a 5 ml nickel column (Macherey-Nagel). The protein was eluted from the nickel column with solubilization buffer supplemented with 500 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins from the soluble fraction were purified by affinity chromatography with Protino Ni-TED PackedColumns2000 (Macherey-Nagel, Düren, Germany) based on the His-tag tail ...
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bioRxiv - Biochemistry 2023Quote: ... The BoNT-X/NTNH complex was purified by gravity-flow affinity chromatography on Ni-NTA agarose resin (Macherey-Nagel, Düren, Germany) in lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... embryos were ground in Tri-Reagent and RNA was purified using affinity chromatography (Nucleospin RNA Clean up kit, Macherey-Nagel, Duren, Germany). Potential contaminating DNA was removed by digestion with rDNase then RNA was purified using Beckman Coulter’s solid-phase reversible immobilization (SPRI ...
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Ni-IDA affinity beads (Macherey-Nagel, Bethlehem, PA, USA) were used to purify TBC-DEG assemblies ...
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bioRxiv - Microbiology 2022Quote: ... The clarified lysate was subjected to affinity chromatography on Ni2+ agarose resin (Macherey-Nagel) and washed extensively with lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: ... purified (Macherey-Nagel #740416.10), and packaged into AAV8 vector (packaging was done by University of Helsinki AAV core unit) ...
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bioRxiv - Bioengineering 2019Quote: ... purified (Macherey-Nagel, 740609) and validated via Sanger sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... amplified and purified (Macherey-Nagel). Plasmid concentrations were measured using a Nanodrop (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... and column purified (Macherey-Nagel) before being transform in electrocompetent Stbl4 bacteria (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... PCR-column purified (Macherey-Nagel), and sent for Sanger sequencing at Genewiz with the primers in Table 2.
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bioRxiv - Microbiology 2024Quote: ... purified (NucleoSpin Plasmid, Macherey-Nagel) and verified by DNA sequencing ...
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bioRxiv - Immunology 2019Quote: ... column purified (Macherey-Nagel, Bethlehem, PA) and were sequenced on an ABI 3730 at Genewiz ...
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bioRxiv - Microbiology 2021Quote: ... and column purified (Macherey-Nagel, Germany). Primers contained a 5’-overhang compatible with Illumina NGS library preparation ...
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bioRxiv - Microbiology 2021Quote: ... and then column purified (Macherey-Nagel). The N expression plasmid was linearized using EcoRV-HF (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... Released DNA was purified (Macherey-Nagel ™ NucleoSpin ™ gel and PCR cleaning kit ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR purified (Macherey-Nagel, 740609.50). The two purified fragments were then ligated using T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... purified PCR fragment (purified using Nucleospin® Gel and PCR Clean-up, MACHEREY-NAGEL GmbH & Co. KG), and H2O to acquired final volume ...
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bioRxiv - Genomics 2020Quote: ... and purified it using columns (Macherey-Nagel). We put the mutagenized genes in a pIVEX vector via Gibson assembly (NEB Hi-Fi DNA assembly ...
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bioRxiv - Developmental Biology 2021Quote: ... and then purified by column (Macherey-Nagel) as template ...
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bioRxiv - Immunology 2020Quote: ... PCR products were purified (MACHEREY-NAGEL, 740609.250), digested with the appropriate restriction enzymes (New England Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: ... purified with NucleoSpin RNA columns (Macherey-Nagel), quantified by Nanodrop One (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplicons were purified (Macherey-Nagel #740609) and sent for Sanger sequencing (Eurofins genomics) ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred to a fresh falcon and directly used for immobilized metal affinity chromatography (IMAC) using the Äktapure25 system with a sample pump (Cytiva) and 1ml Ni-NTA columns (Macherey-Nagel). Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore ...
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bioRxiv - Developmental Biology 2024Quote: ... The His6-3C protease was then removed from the sample by immobilized metal affinity chromatography using a 1 mL Protino Ni-NTA column (Macherey-Nagel). The mTRIM66 PHD-bromo-domain protein was further purified by size exclusion chromatography using a HiLoad 16/600 Superdex 75 pg column (Cytiva ...
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bioRxiv - Bioengineering 2022Quote: ... purified using PCR clean-up kit (Macherey-Nagel), and transformed in electrocompetent E ...
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bioRxiv - Microbiology 2022Quote: ... metagenomic DNA was purified on columns (Macherey-Nagel) after mechanical lysis by bead-beating ...
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bioRxiv - Microbiology 2021Quote: ... RNA was purified using NucleoSpin RNA (Macherey-Nagel) and DNase-treated (RNaseOUT ...
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bioRxiv - Cell Biology 2020Quote: ... DNA fragments were purified using NucleoSpin (Macherey-Nagel) and diluted to 1/100 for input and to the half for immunoprecipitated fractions ...
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bioRxiv - Biochemistry 2022Quote: ... and column purified (cat no 740.948, Macherey-Nagel), following manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... and column purified (cat no 740.948, Macherey-Nagel).
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bioRxiv - Molecular Biology 2023Quote: ... purified using Nucleospin kit (Macherey-Nagel, Düren, Germany), and subcloned into pGEM-T plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of respective proteins from bacterial extracts was performed by affinity chromatography using Protino glutathione-conjugated agarose 4B (Macherey-Nagel, Düren, Germany). Except for GST-WAVE2-WCA ...
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bioRxiv - Genomics 2022Quote: ... the DNA was purified using NucleoSpin columns (Macherey-Nagel) and sequenced on a NextSeq 500 Illumina sequencer.
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bioRxiv - Neuroscience 2020Quote: ... The PCR products were gel purified (Macherey-Nagel, USA) and used for ligation reaction ...
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bioRxiv - Cell Biology 2021Quote: ... Fragments were gel purified (following kit instructions, Macherey-Nagel) and 30 ng of insert and 10 ng of vector ligated using 6U of T4 DNA ligase (Thermo ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products from genotyping were purified (Macherey-Nagel) and sequenced (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products were purified with Nucleospin (Macherey-Nagel) as per manufacturer’s instructions and sequenced with Sanger sequencing (Eurofins or Genscript).
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bioRxiv - Microbiology 2021Quote: ... purified (gel and PCR clean-up kit, Macherey-Nagel) and subsequently sequenced by the use of primers oRH068 ...
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bioRxiv - Biophysics 2021Quote: ... and purified by Ni-IDA-chromatography (Protino, Macherey-Nagel) and anion-exchange chromatography (Resource™ Q ...