Labshake search
Citations for Macherey-Nagel GmbH :
1 - 50 of 444 citations for Recombinant Mouse Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
-
bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
-
bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
-
bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
-
bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
-
bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
-
bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
-
bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
-
bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
-
bioRxiv - Cancer Biology 2023Quote: RNA was isolated from Kasumi-1 cells after 2 days after shRUNX1::ETO knockdown was induced with doxycycline using the Nucleospin RNA kit (Macherey-Nagel). cDNA was synthesised using Superscript II (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
-
bioRxiv - Plant Biology 2023Quote: ... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
-
bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
-
bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
-
bioRxiv - Microbiology 2023Quote: ... Infectious KSHV recombinants were generated by transfection of purified bacmid DNA (NucleoBond Xtra Midi (Macherey-Nagel)) into iSLK cells using GenJet Ver ...
-
bioRxiv - Genomics 2020Quote: Total mRNA of edited HUDEP-2 cells was isolated using the NucleoSpin RNA kit (Macherey-Nagel) and reverse transcribed into cDNA ...
-
bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
-
bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
-
bioRxiv - Plant Biology 2022Quote: ... undecanal-tagged peptides and excess reagents were removed by passing the reaction through HRX-reverse phase spin-columns (Macherey-Nagel GmbH & Co, KG, Dueren, Germany). The flow-through containing the enriched N-termini were dried with a SpeedVac to a remaining volume of ~50 μL ...
-
bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
-
bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
-
bioRxiv - Microbiology 2023Quote: Total RNA was prepared from isolated colonic epithelial cells of Hsp60Δ/ΔIEC and Hsp60fl/fl mice on day 2 with the column-based NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
-
bioRxiv - Plant Biology 2021Quote: ... an EC 100/2 Nucleoshell Phenyl-Hexyl column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
-
bioRxiv - Microbiology 2020Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA genes of AOB and AOA was amplified with primers amoA-1F/amoA-2R (66 ...
-
bioRxiv - Immunology 2022Quote: Total RNA was isolated from 2 × 105 cells untreated or butyrate-conditioned BMDMs using the NucleoSpin RNA extraction kit (Macherey-Nagel, 740902.50) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA gene of Ca ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
-
bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated for 1 hour with 2 g Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany). The matrix was washed extensively with buffer L and bound protein was eluted in buffer L containing 250 mM imidazole ...
-
bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
-
bioRxiv - Genetics 2023Quote: ... and then incubated for 2 h on ice on a rocking platform with 1 ml Protino Ni-NTA Agarose (MN, Macherey-Nagel GmbH, Düren, Germany) pre-equilibrated with lysis buffer ...
-
bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was first extracted from 1 x 105 cells using NucleoSpin Tissue (MACHEREY-NAGEL #740952) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... Amino acids were separated on a reverse-phase C18 column (EC 250/2 NUCLEOSIL 100-3 C18HD, L=250 mm, ID: 2 mm; Macherey-Nagel). Samples (5 µL ...
-
bioRxiv - Biochemistry 2023Quote: ... 2 μL sample volume was injected into a Nucleodur HILIC column (250 mm * 2 mm, 3 μm particle size, Macherey-Nagel). Metabolites were eluted at 20 °C with an acetonitrile gradient (90% (v/v ...
-
bioRxiv - Plant Biology 2020Quote: ... LMW carbonyls were separated using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an Ekspert ultraLC 100 UHPLC system (AB SCIEX ...
-
bioRxiv - Plant Biology 2023Quote: ... Separation was carried out using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an ekspert ultraLC 100 UHPLC system coupled to a QTRAP 4500 mass spectrometer (AB SCIEX ...
-
bioRxiv - Systems Biology 2019Quote: ... RNA was recovered from 1 x 106 cells using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The RNA was sent to Eurofins for RNA-seq analysis ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from around 5×105 to 1×106 cells using the NucleoSpin RNA Plus (MACHEREY-NAGEL, 740984.50) or Quick-RNA Miniprep Kit (Zymo Research ...
-
bioRxiv - Plant Biology 2020Quote: ... Nucleosil 100-5 N(CH3)2 column (Macherey-Nagel GmbH & Co, Düren, Germany) and eluted isocratically with 100% methanol containing 0.01% acetic acid at a flow rate of 1 ml min-1 ...
-
bioRxiv - Neuroscience 2022Quote: Cells were lysed (RLT buffer with 1% β-mercaptoethanol) and RNA was isolated via column purification (NucleoSpin RNA kit, Macherey-Nagel 740955). RNA integrity and concentration were determined with BioAnalyzer (Agilent ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from INS-1 832/13 cells 48h after transfection using the NucleoSpin extraction kit (Macherey-Nagel, Düren, Germany). The amount of isolated RNA was measured using the NanoDrop system ...
-
bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from primary mouse hepatocytes using NucleoSpin kit (Macherey-Nagel cat# 740955.25) according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... we extracted DNA from liver tissue of a single mouse using a Nucleospin Tissue Kit (Macherey-Nagel) and quantified the DNA with a QuantiFluor dsDNA System (Promega ...
-
bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse tissues using the Nucleospin RNA Plus kit (Macherey-Nagel, cat# 740984.50), following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: ... a Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
-
bioRxiv - Developmental Biology 2022Quote: ... The supernatant was collected and loaded onto 2 mL pre-equilibrated Ni-NTA agarose (Macherey-Nagel). The mixture was rotated at 4 □ for 2 h ...