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Citations for Macherey-Nagel GmbH :
1 - 30 of 30 citations for Recombinant Human Epidermal Growth Factor Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
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bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
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bioRxiv - Microbiology 2023Quote: ... Infectious KSHV recombinants were generated by transfection of purified bacmid DNA (NucleoBond Xtra Midi (Macherey-Nagel)) into iSLK cells using GenJet Ver ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Plant Biology 2022Quote: ... undecanal-tagged peptides and excess reagents were removed by passing the reaction through HRX-reverse phase spin-columns (Macherey-Nagel GmbH & Co, KG, Dueren, Germany). The flow-through containing the enriched N-termini were dried with a SpeedVac to a remaining volume of ~50 μL ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted for Termignon cheese isolates and all new strains sequenced using the Illumina technology for this project from fresh haploid mycelium and conidia after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel). Sequencing was performed using the Illumina paired-end technology (Illumina Inc. ...
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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bioRxiv - Physiology 2022Quote: ... Human keratinocyte RNA was purified using NucleoSpin RNA (Macherey-Nagel, Düren, Germany). Before the RNA preparation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... were extracted from fresh haploid mycelium after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel, Düren, Germany). Sequencing was performed using the Illumina HiSeq 2500 paired-end technology (Illumina Inc. ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from 50 mg of frozen and fine ground rosette leaf material from four biological replicates per genotype and growth condition using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from fresh mycelium and conidia suspension after single-ascospore isolation and growth for five days on MEA media using the Nucleospin soil kit (Macherey-Nagel, Düren, Germany). For checking that isolated spores were recombinant offspring and not asexual conidia ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA from human islets was extracted using Nucleospin RNA XS (Macherey-Nagel) three days post-transfection.
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bioRxiv - Microbiology 2022Quote: ... −20M and GLT1cc infected human liver chimeric mice using the NucleoSpin Virus kit (Macherey-Nagel) according to the manufacturer’s instructions.
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jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: Total RNA from human cell lines were extracted using NucleoSpin RNA Plus columns (Macherey-Nagel, France), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Total RNAs from cultured human iPS cells were extracted using NucleoSpin RNA Plus XS (Macherey-Nagel). The 100 ng of total RNAs were used as starting materials to generate RNA-seq libraries with the TruSeq Stranded mRNA LT sample prep kit (Illumina) ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from six human fecal samples using the NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Germany), as described previously [36] ...
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bioRxiv - Immunology 2021Quote: ... apical washes of Human coronavirus 229E (HCoV-229E) and MERS-CoV-infected hAECs were extracted using the NucleoMag VET (Macherey-Nagel) according to manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from snap frozen mouse or human liver tissue using NucleoSpin RNA Mini kit (Macherey-Nagel, Düren, Germany). First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen) ...