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Citations for Macherey-Nagel GmbH :
1 - 45 of 45 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
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bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
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bioRxiv - Microbiology 2023Quote: ... Infectious KSHV recombinants were generated by transfection of purified bacmid DNA (NucleoBond Xtra Midi (Macherey-Nagel)) into iSLK cells using GenJet Ver ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Plant Biology 2022Quote: ... undecanal-tagged peptides and excess reagents were removed by passing the reaction through HRX-reverse phase spin-columns (Macherey-Nagel GmbH & Co, KG, Dueren, Germany). The flow-through containing the enriched N-termini were dried with a SpeedVac to a remaining volume of ~50 μL ...
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bioRxiv - Microbiology 2019Quote: ... and 48 h later RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel). RNA levels were determined with Power SYBR® Green RNA-to-CT™ 1-Step Kit using a StepOne Plus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... nidulans mycelia after 9 h incubation using a NucleoSpin RNA Plant kit (Macherey-Nagel) and RNase-Free DNase (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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bioRxiv - Physiology 2022Quote: ... Human keratinocyte RNA was purified using NucleoSpin RNA (Macherey-Nagel, Düren, Germany). Before the RNA preparation ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA from human islets was extracted using Nucleospin RNA XS (Macherey-Nagel) three days post-transfection.
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Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: ... infiltrated leaves were harvested after 6 h for total RNA isolation with NucleoSpin® RNA Plus Kit (Macherey-Nagel). First strand cDNA was synthesized with RevertAid™ M-MuLV reverse transcriptase (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... −20M and GLT1cc infected human liver chimeric mice using the NucleoSpin Virus kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 16 h after transfection and total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel).
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bioRxiv - Molecular Biology 2022Quote: ... single colonies were picked and transferred into bacterial starter cultures and incubated for ∼6 h for mini-prep (Macherey-Nagel, NucleoSpin Plasimd Mini kit for plasmid DNA ...
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jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: Total RNA from human cell lines were extracted using NucleoSpin RNA Plus columns (Macherey-Nagel, France), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Total RNAs from cultured human iPS cells were extracted using NucleoSpin RNA Plus XS (Macherey-Nagel). The 100 ng of total RNAs were used as starting materials to generate RNA-seq libraries with the TruSeq Stranded mRNA LT sample prep kit (Illumina) ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Immunology 2020Quote: Total cellular RNA (containing viral genomic RNA) was extracted 48 h after transfection using NucleoSpin RNA Plus kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... genomic DNA was extracted from bacterial strains grown on King’s medium B (King et al. 1954) at 28°C for 24 h using NucleoSpin Microbial DNA kit (Macherey-Nagel, Germany). The quality of the genomic DNA was assessed by electrophoresis in 0.8% agarose gel.
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bioRxiv - Immunology 2022Quote: ... The surviving cells were expanded for 24 h and DNA was extracted using a NucleoSpin Tissue kit (Macherey-Nagel, Cat#740952). An additional eight biological replicates were similarly prepared but did not undergo killing by TC5A87 ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were extracted from mid-log yeast cultures grown 16 h at 20°C in YPD as described above using the Nucleospin RNA II kit (Macherey-Nagel) and were reverse transcribed with Superscript-II reverse transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were extracted from agarose gel (1.5%, 1 h, 100 V) by using NucleoSpin® Gel and PCR Cleanup XS kit (Macherey-Nagel) and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from control and IMCD PCM1 KO cells before SAG treatment and 24 h after SAG treatment using NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Physiology 2024Quote: ... standard incubation control and after 1,2,4 h at hypoxic conditions) was extracted using the NucleSpin RNA Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Reaction mixtures were incubated at 40°C for 4 h followed by 12 h incubation at 12°C and applied at 120 nl/s to NANO-ADAMANT HP-TLC plates (Macherey-Nagel, Germany) with a CAMAG Linomat 5 TLC sampler (CAMAG ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: ... The three constructs were then cultured for 24 h at 37°C and purified with NucleoBond Xtra Midi Kit (Macherey-Nagel, Germany) and used for gene reporter assay.
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bioRxiv - Microbiology 2020Quote: DNA was extracted from six human fecal samples using the NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Germany), as described previously [36] ...
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bioRxiv - Genetics 2023Quote: ... and then incubated for 2 h on ice on a rocking platform with 1 ml Protino Ni-NTA Agarose (MN, Macherey-Nagel GmbH, Düren, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Base-editing efficiency when targeting a plasmid-borne gene was estimated by isolating plasmid DNA upon 24-h editing treatment by using the NucleoSpin™ plasmid EasyPure kit (Macherey-Nagel, Dueren, Germany). Either the isolated plasmids or the purified PCR products from independent samples were Sanger-sequenced using the Mix2Seq™ kit (Eurofins Scientific ...
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bioRxiv - Immunology 2021Quote: ... apical washes of Human coronavirus 229E (HCoV-229E) and MERS-CoV-infected hAECs were extracted using the NucleoMag VET (Macherey-Nagel) according to manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from snap frozen mouse or human liver tissue using NucleoSpin RNA Mini kit (Macherey-Nagel, Düren, Germany). First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen) ...