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Citations for Macherey-Nagel GmbH :
1 - 50 of 260 citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
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bioRxiv - Microbiology 2020Quote: ... ii) Nucleomag Vet kit (Macherey-Nagel) or MagAttract 96 cador Pathogen Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
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bioRxiv - Genetics 2022Quote: ... a NucleoSpin Plant II kit (Macherey-Nagel) for the Seven-parent-MAGIC and NORFAB panels ...
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bioRxiv - Microbiology 2022Quote: ... Filter pieces were transferred into MN Bead Tubes Type A (Macherey-Nagel) containing 800 μl of buffer RLT Plus that were pre-chilled at −80°C for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Plant Biology 2023Quote: ... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or the NucleoSpin Plant II kit (Macherey-Nagel).
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bioRxiv - Bioengineering 2019Quote: ... filtered over a 0.45 μm filter (type PA-45/25, Macherey-Nagel, Germany), and diluted with Milli-Q water to reach the desired concentration range for quantification between 1 and 100 mg L−1 ...
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bioRxiv - Bioengineering 2019Quote: ... filtered over a 0.22 µm filter (type PA-45/25, Macherey-Nagel, Germany) and diluted with Milli-Q water to reach the desired concentration range for quantification between 1 and 100 mg L−1 ...
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bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from blood samples of patients II-8 and IV-1 using the Nucleospin extraction kit (ref 740952-Macherey-Nagel). Subsequently ...
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bioRxiv - Molecular Biology 2019Quote: ... the Nucleospin RNA II kit (Macherey-Nagel, Duren, Germany) was used for total RNA extraction following the manufacturer’s instruction (Gondard et al. ...
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bioRxiv - Immunology 2022Quote: For RNA extraction NucleoSpin RNA II kit (Macherey-Nagel) was used ...
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bioRxiv - Microbiology 2023Quote: ... Infectious KSHV recombinants were generated by transfection of purified bacmid DNA (NucleoBond Xtra Midi (Macherey-Nagel)) into iSLK cells using GenJet Ver ...
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bioRxiv - Bioengineering 2021Quote: ... followed by filtration over a 0.45 μm filter (type PA-45/25, Macherey-Nagel, Germany) to remove all solids ...
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bioRxiv - Plant Biology 2020Quote: ... purified using Nucleospin Extract II kit (Macherey-Nagel, Düren, Germany) and rigorously quantified through UV (260 nm ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated using NucleoSpin RNA II (Macherey-Nagel) and reverse transcribed using Superscript III Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... gondii tachyzoites using the Nucleospin RNA II kit (Macherey-Nagel). Reverse transcription was performed with the Superscript III first-strand synthesis kit (Invitrogen) ...
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A systematic approach identifies p53-DREAM target genes associated with blood or brain abnormalitiesbioRxiv - Genetics 2023Quote: Total RNAs were extracted using nucleospin RNA II (Macherey-Nagel), reverse-transcribed using superscript IV (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNAs were extracted using nucleospin RNA II (Macherey-Nagel), reverse-transcribed using superscript IV (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted with the NucleoSpin II kit (Macherey-Nagel) and reverse-transcribed using the High- Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans animals were transferred to 2 ml MN Bead Tubes Type A (Macherey-Nagel, Düren, Germany) and lysed using a Precellys Bead Beating system with an additional Cryolys cooling module (Bertin Instruments ...
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bioRxiv - Physiology 2021Quote: ... mRNA specimens were isolated from the cells grown on collagen-gel in 24-well dishes using a NucleoSpin RNAplus RNA isolation kit (Macherey-Nagel, Dueren, Germany) and converted to cDNA with SuperScript IV VILO Master Mix (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was isolated using NucleoSpin RNA II Kit (Macherey-Nagel) in pursuance of the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was extracted using NucleoSpin® Plant II (Macherey-Nagel), and submitted to The Biotech Center ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted using the RNA NucleoSpin II kit (Macherey-Nagel) and treated with DNase (Ambion Turbo DNA-free ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated with NucleoSpin RNA II Kit (Macherey-Nagel) according to the manufacturer’s protocol (MN ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA wasisolated using the Nucleospin Plant II kit from Macherey-Nagel. Isolated genomic DNA was G-tailed at random DNA breaks using dGTP together with terminal transferase (NEB cat M0315S ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Microbiology 2023Quote: ... The filters were then individually placed into 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel), added with 1,200 µL MWA1 lysis buffer and stored at - 80°C until nucleic acids extraction as specified in the corresponding section below.
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted from wild-type worms using the Nucleospin RNA® kit (Macherey-Nagel – 740955) or Trizol Reagent™ (Ambion® ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted using the Nucleospin® plant II (Macherey-Nagel) following the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2020Quote: DNA extractions were performed using NucleoSpin® Plant II Mini (Macherey-Nagel) as per manufacturer’s instructions with the following minor modifications ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using the Plant NucleoSpin II kit (Macherey-Nagel) according to manufacturer’s specifications.
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated using the Plant NucleoSpin II kit (Macherey-Nagel) according to manufacturer’s specifications.
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA was extracted using NucleoSpin plant II kit from Macherey-Nagel following the instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and purified using the NucleoSpin RNA II Kit (Macherey-Nagel, Duren, Germany).
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated using the Nucleospin RNA II kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted using the Nucleospin RNA II kit (Macherey-Nagel). cDNA was synthesised using the RevertAidHminus First Strand cDNA synthesis kit (Thermo Fisher Scientific ...