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Citations for Macherey-Nagel GmbH :
1 - 50 of 53 citations for Recombinant E. coli LT IIa His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Biochemistry 2022Quote: ... coli using NucleoBond Xtra Maxi columns (Macherey-Nagel) followed by isopropanol precipitation ...
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bioRxiv - Neuroscience 2023Quote: ... coli was done using NucleoSpin Plasmid (MACHEREY-NAGEL). The mutations were verified by sequencing (Microsynth ...
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bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
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bioRxiv - Microbiology 2020Quote: ... coli using NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, Germany). Deletion of the degU ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cultures using NucleoBond Plasmid MAXI KIT (Macherey-Nagel) and resuspended in TE buffer ...
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bioRxiv - Microbiology 2022Quote: ... coli using the Nucleospin RNA Extraction Kit (Macherey-Nagel) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... coli clones using a Miniprep kit (Macherey-Nagel, 740588) and verified by single pass primer extension sequencing (ACGT Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH10 cells using a BAC prep kit (Macherey-Nagel). P19 Copg1-/- cells were transfected with 1µg BAC DNA using Effectene (QIAGEN) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli was performed using NucleoSpin Plasmid kit from Macherey-Nagel. DNA fragments were purified from agarose gels using the NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cultures using a NucleoSpin Plasmid kit (Macherey-Nagel, Germany), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... coli hosts containing DCFHP genes were maxi-prepped (Macherey-Nagel). HD-BIOP3 cells were then transfected with both components and underwent selection by glutamine deprivation.9 Resulting pooled cells were sorted and resulting single clones were analyzed as described throughout the manuscript ...
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bioRxiv - Microbiology 2022Quote: ... coli stock using the Nucleospin plasmid kit (Macherey-Nagel, Germany) and the PCR amplicon used was generated using C ...
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bioRxiv - Cancer Biology 2024Quote: ... coli using the Nucleobond Xtra Maxi EF Kit (Macherey-Nagel) and adequate representation of sgRNAs in the library was verified by next-generation sequencing and analysis with the python script count_spacers.py (Joung et al. ...
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bioRxiv - Neuroscience 2023Quote: ... coli transformed clones using the NucleoBond Xtra Midi Kit (Macherey-Nagel), according with the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... coli cultures using the NucleoSpin Plasmid kit (Macherey-Nagel, Cat.#740588.50). All plasmids contained ampicillin resistance (ampR ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
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bioRxiv - Biochemistry 2022Quote: ... Coli using NuceloBond Xtra Maxi Kit per the manufacturers recommendations (Macherey-Nagel). All scFv-ACE2 ...
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bioRxiv - Immunology 2021Quote: ... coli cultures and purified using Nucleobond Xtra maxi EF columns (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... coli and purified using Protino Ni-IDA Resin (Macherey-Nagel, Düren, Germany). The EMSA experiments were conducted as described previously (Wu et al ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (DE3) and affinity purified using Protino Ni-NTA Agarose (Macherey-Nagel). Subsequently ...
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bioRxiv - Genetics 2019Quote: ... coli BL21-Gold cells and purified on Ni-NTA Agarose (Macherey-Nagel). Two guinea pigs were immunized at Bioscience with His-DBN-1(1-132 ...
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bioRxiv - Biophysics 2022Quote: ... coli MC1061 cell line using the Nucleobond Xtra Maxi Kit (Macherey-Nagel). 1.3 μg DNA was used per 106 cells ...
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bioRxiv - Biochemistry 2020Quote: ... coli NM522 cells then extracted using a NucleoBond Xtra Midi kit (Macherey-Nagel). Quantification was performed using a SimpliNano spectrophotometer (Biochrom).
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bioRxiv - Microbiology 2020Quote: ... coli DH5α strains using the NucleoSpin® Plasmid DNA Purification kit (Macherey-Nagel). Cloning was performed using the appropriate restriction endonucleases and T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α cells for maxiprep using the NucleoBond Xtra Maxi kit (Macherey-Nagel). Linear DNA fragments used as expression templates in the cell-free reactions were PCR amplified using primers and templates listed in Sup Table 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... coli under chloramphenicol selection and purified with the Endotoxin-Free MaxiPrep kit (Macherey-Nagel).
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bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
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bioRxiv - Cell Biology 2023Quote: ... coli overnight culture (37°C, 130 rpm) via the NucleoBond Xtra Midi Kit (Macherey-Nagel) and precipitated by supplementing 0.1x volume 3 M sodium acetate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... coli bacterial cultures using NucleoSpin Plasmid QuickPure or NucleoBond Xtra MidiPrep kits (Macherey-Nagel, Düren, Germany) according to manufactureŕs instructions ...
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bioRxiv - Microbiology 2023Quote: ... Infectious KSHV recombinants were generated by transfection of purified bacmid DNA (NucleoBond Xtra Midi (Macherey-Nagel)) into iSLK cells using GenJet Ver ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Genetics 2021Quote: ... coli (High Efficiency) cells and subsequent plasmid isolation was performed using the NucleoSpin Plasmid kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Immunology 2022Quote: ... coli strain in presence of 100 μg/ml ampicillin and prepared by Midi DNA-prep kit (Macherey-Nagel, 740410) for the transient transfection ...
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bioRxiv - Plant Biology 2022Quote: ... undecanal-tagged peptides and excess reagents were removed by passing the reaction through HRX-reverse phase spin-columns (Macherey-Nagel GmbH & Co, KG, Dueren, Germany). The flow-through containing the enriched N-termini were dried with a SpeedVac to a remaining volume of ~50 μL ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA constructs for human Parkin mutant expression were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA constructs for mammalian tissue culture (Supplementary Table 1) were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Biochemistry 2023Quote: cDNA constructs for mammalian tissue culture were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (no. 740410.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...