Labshake search
Citations for Macherey-Nagel GmbH :
1 - 50 of 119 citations for Chikungunya Virus E1 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... viral DNA was extracted from equal amounts of virus using a Nucleospin virus kit (MACHEREY-NAGEL, 740983.10). To account for potential differences in efficiency of DNA extraction ...
-
bioRxiv - Genomics 2022Quote: ... using the Nucleospin virus kit (Macherey-Nagel). Purified DNA was subsequently used for PCR amplification with the following primers ...
-
bioRxiv - Microbiology 2023Quote: ... and NucleoSpin Virus (Macherey-Nagel, Düren, Germany). DNA was quantified and the 260 nm/280 nm ratio was determined using a NanoDrop ND-1000 (Thermo Fisher Scientific Inc. ...
-
bioRxiv - Microbiology 2023Quote: ... the NucleoSpin RNA Virus (Macherey-Nagel GmbH) or by semiautomated RNA extraction using the Easymag (BioMerieuxTM) ...
-
bioRxiv - Microbiology 2022Quote: ... using the NucleoSpin RNA virus kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... or NucleoSpin 96 Virus (Macherey-Nagel, Duren, Germany) following the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2022Quote: ... NucleoSpin RNA virus kit (Macherey-Nagel; cat no. 740956.250) was used for RNA extraction.
-
bioRxiv - Microbiology 2024Quote: ... by using the NucleoSpin RNA virus kit (Macherey-Nagel). For intracellular viral RNA cells were harvested in TRI Reagent and RNA extraction (Zymo Research ...
-
bioRxiv - Cancer Biology 2023Quote: ... the cultured alone C4-2B cells or the mixed co-cultured C4-2B/MC3T3-E1 were harvested for DNA extraction using the NucleoSpin Tissue kit (Macherey-Nagel Inc.). Primers against the nuclear and mitochondrial genomes were used for qRT-PCR (sequences provided in Supplementary Table S2 ...
-
bioRxiv - Microbiology 2022Quote: ... or NucleoSpin® RNA Virus (Macherey-Nagel GmbH, Düren, Germany). BHK-21 cells were infected with VSV-G-3CLpro-L wild type and F305L (3CLpro ...
-
bioRxiv - Cell Biology 2023Quote: ... or from supernatants using NucleoSpin RNA virus kit (Macherey-Nagel). Reverse transcription and quantitative real-time PCR was performed using Luna Universal One-Step RT-qPCR Kit (New England Biolabs ...
-
bioRxiv - Immunology 2019Quote: ... Virus RNA was extracted from supernatant using NucleoSpin RNA kit (Macherey-Nagel) and subsequently quantified quatified using qPCR with KAPA Fast Universal one-step qRT-PCR kit (KAPA ...
-
bioRxiv - Microbiology 2023Quote: ... RNA was harvested using the NucleoSpin RNA Virus kit (MACHEREY-NAGEL, 740956) following manufacturer’s instructions and reverse-transcribed with SuperScript IV (Invitrogen ...
-
bioRxiv - Biochemistry 2021Quote: ... total RNA from HEK293 cells were isolated using the NucleoSpin RNA kit (Macherey-Nagel, Bethlehem, PA). cDNA was synthesized using the iScript kit (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... total RNAs were isolated from HeLa and HEK293 cells using the NucleoSpin RNA kit (Macherey-Nagel). To examine endogenous mRNA expression levels and the splicing products ...
-
bioRxiv - Microbiology 2022Quote: ... extracellular viral RNA was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and intracellular viral RNA using the E.Z.N.A Total RNA Kit I (Omega Bio-tek) ...
-
bioRxiv - Molecular Biology 2022Quote: Viral RNA isolation was performed with the NucleoSpin RNA Virus kit (Macherey-Nagel, Germany) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed using the NucleoSpin RNA Virus Kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... the DNA of HEK293 cells was extracted and purified following the NucleoSpin Tissue protocol (Macherey-Nagel, Düren, Germany). The genotype at rs1047626 was verified to be heterozygote (AG ...
-
bioRxiv - Microbiology 2021Quote: ... viral RNA was isolated with the NucleoSpin RNA Virus kit (Macherey-Nagel, Leiden, The Netherlands) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... −20M and GLT1cc infected human liver chimeric mice using the NucleoSpin Virus kit (Macherey-Nagel) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... Viral RNA in supernatant cell culture was isolated with the Nucleospin Dx virus (Macherey-Nagel). Reverse transcription was performed with random primers and Superscript IV (Life Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... The viral RNA in each sample was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and quantified by qRT-PCR.
-
bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from tissues using a NucleoSpin RNA Virus mini kit (Macherey-Nagel, 740956) at SCVL ...
-
bioRxiv - Microbiology 2020Quote: RNA was extracted from 50µl aliquots of supernatant using the Nucleospin 96 virus kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... see Addgene #98034 for reference sequence) virus samples using a Nucleospin RNA kit (Macherey-Nagel, Germany) and treated with DNase I (37°C for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Viral RNA was extracted from the cell culture supernatant using the NucleoSpin 96 Virus kit (Macherey-Nagel). Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
-
bioRxiv - Immunology 2022Quote: Viral RNA was extracted from cell culture supernatants using the NucleoSpin RNA virus isolation kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... and total RNA was extracted by using NucleoSpin Plus RNA virus Kit (Macherey-Nagel, cat no. 740984.10). For serum (50 μl) ...
-
bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from cell culture fluids using the NucleoSpin RNA Virus Kit (Macherey-Nagel, Germany). Total RNA from cells was isolated using TRIreagent (Sigma ...
-
bioRxiv - Microbiology 2022Quote: Viral RNA from the supernatant was extracted using the NucleoSpin RNA Virus Isolation Kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA of progeny virions was extracted from supernatants using the NucleoSpin RNA virus kit (Macherey-Nagel, #740956) according to the manufacturer’s instructions and stored at −80 °C until PCR-based analysis.
-
bioRxiv - Molecular Biology 2024Quote: ... A 200 ul aliquot was used for RNA extraction using a NucleoMag Virus kit (Macherey-Nagel, Düren, Germany) and a Magnetic Particle Processor (MPP ...
-
bioRxiv - Systems Biology 2020Quote: The viral RNA from supernatant of infected cells was isolated using the NucleoSpin RNA virus isolation kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... The vRNA in the cell culture supernatant was purified using a NucleoSpin® RNA virus kit (Macherey-Nagel, 740956) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Total cellular RNA from mock and virus-infected hAEC cultures was extracted with the NucleoMag RNA kit (Macherey-Nagel) according manufacturers guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
-
bioRxiv - Microbiology 2020Quote: RNAs from mock infected or infected (MOI=0.001) cell culture supernatant were extracted using the Nucleospin Dx Virus RNA purification kit (Macherey-Nagel). Then qRT-PCR was performed in triplicate as described20 ...
-
bioRxiv - Microbiology 2022Quote: RNAs from mock infected or infected (MOI=0.01) A549-hACE2 cell culture supernatant were extracted using the Nucleospin Dx Virus RNA purification kit (Macherey-Nagel). Then qRT-PCR was performed in triplicate as described20 ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2021Quote: Viral RNA was isolated from 4 µl of a typical lentivirus preparation (∼2×106 TU/µl) using the NucleoSpin RNA Virus mini kit (Macherey-Nagel). Purified RNA was used as a template for reverse transcription using the SuperScript VILO cDNA Synthesis Kit (Invitrogen ...
-
bioRxiv - Neuroscience 2019Quote: RNA viral genomes were extracted from two Tripodi lab (EnvA/ SiR-CRE and EnvA/Sir-FLPo) and one Wickersham lab (RVΔG-4mCherry) rabies virus samples using a Nucleospin RNA kit (Macherey-Nagel, Germany) and treated with DNase I (37°C for 1 hour ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... isolation of vRNA from 150 µL cell-free supernatants was carried out with the NucleoSpin RNA virus kit (Macherey-Nagel, 740956) as described in the manufacturers’ instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... The viruses were characterized by isolating viral RNA from 150-µL aliquots of the cell culture supernatant with the NucleoSpin RNA Virus kit (Macherey-Nagel) followed by conventional RT-PCR and bidirectional Sanger sequence analysis of the inserted SARS-CoV-2 sequences and flanking regions ...
-
bioRxiv - Immunology 2024Quote: HIV RNA was extracted from plasma samples collected from either HIV-1 infected or uninfected humanized hNSG mice transplanted with CD4+ T cells from PWH in the absence or presence of immunotherapy using NucleospinTM RNA Virus kit (Cultek, Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... 200 μL of the permeabilized sample was then processed using the Machery-Nagel NucleoSpin Virus kit (Macherey-Nagel, Catalog # 740983.50 and 740983.250).
-
bioRxiv - Microbiology 2020Quote: ... Clonal virus populations were characterized by isolating viral RNA from 150 µl aliquots of the cell culture supernatant with the NucleoSpin® RNA Virus kit (MACHEREY-NAGEL) followed by conventional RT-PCR and bidirectional Sanger sequence analysis.
-
bioRxiv - Zoology 2024Quote: ... RNA from 150 µl of excreta solution was extracted by adding 600 µl of RAV1 lysis buffer and using NucleoSpin virus RNA kit (Macherey-Nagel, France).
-
bioRxiv - Neuroscience 2019Quote: ... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...