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Citations for Macherey-Nagel GmbH :
1 - 50 of 2166 citations for 20 Hydroxyecdysone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... silica gel G60 plates (10 x 20 cm, Macherey-Nagel) were used to spot samples and lipids were separated with CHCl3/CH3OH (90:10 ...
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bioRxiv - Genetics 2022Quote: RNA extraction was performed from frozen tissue in culture plates (P6 well plate, Falcon®) by using the NucleoSpin RNA kit (Macherey-Nagel). RNA concentration was measured with NanoDrop1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... were prepared with 20 µL of NucleoMag B-Beads (NucleoMag Pathogen kit, Macherey-Nagel) and 975 µL of Buffer NPB2 (NucleoMag Pathogen kit ...
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bioRxiv - Plant Biology 2020Quote: ... ZT-20 and ZT-24 using the NucleoSpin RNA Plant kit (Macherey-Nagel, Düren, Germany). We performed a DNase I treatment (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from the protoplasts using NucleoBond® CB 20 Kit (Macherey-Nagel, Germany). Concentration ...
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bioRxiv - Genetics 2023Quote: ... NucleoBond AXG 20 columns and NucleoBond buffer kit were purchased from Macherey-Nagel (Bethlehem, PA). Nanosep Centrifugal Devices (MWCO 3K ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Extracted steroids were concentrated under nitrogen and dissolved in 20 µl of trichloromethane for separation by thin-layer chromatography on silica gel TLC plates (Macherey-Nagel, Oensingen, Switzerland). Radiolabeled steroids were quantified by autoradiography on a Fuji FLA-7000 PhosphorImager (Fujifilm ...
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bioRxiv - Microbiology 2019Quote: ... following incubation with 20 mg/ml lysozyme in P1 buffer (NucleoSpin® Plasmid Kit, Macherey-Nagel) overnight at 30 °C ...
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bioRxiv - Immunology 2022Quote: ... BEAS-2B and MRC-5 using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel). One-Step RT-PCR SuperScript™ III Platinum™ Kit (Life Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... thaliana 5-days-old seedlings was isolated using NucleoSpinTM RNA Plant Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell aggregates were lysed on plate using 350 μL RA1 (NucleoSpin® RNA kit (Macherey-Nagel) buffer with 3,5 μL β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... 20 µL of proteinase K (20 mg/mL; MACHEREY-NAGEL # 740506) was added and the mixture incubated at 70°C for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... and EBs at day 5 using the NucleoSpin RNA Plus Kit (Macherey-Nagel, Düren, Germany) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted DNA from fresh spores stored at -20°C using the Nucleospin Soil Kit (Macherey-Nagel, Germany). Genomes were sequenced using Illumina 150 bp paired-end sequencing technology at 46X coverage on average ...
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bioRxiv - Genetics 2022Quote: ... Rubus DNA extractions were performed in 96-well plates from freeze-dried tissue using commercial kits (e.g. Macherey-Nagel Nucleospin kit was used at PFR). Mānuka samples were collected in natural stands in remote locations using silica beads in 2mL screw cap tubes as described in Koot et al ...
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bioRxiv - Cancer Biology 2022Quote: ... The library stock was prepared by scraping plates followed by purification using the Midi Prep kit (Macherey-Nagel). Library sequencing was performed on a MiSeq run to ensure sgRNA uniformity ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was isolated from 5 Mio HeLa cells using the NucleoSpin tissue kit (Macherey-Nagel) according to the manufacturers protocol.
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bioRxiv - Plant Biology 2023Quote: DNA was extracted from 20 mg of dried leaf tissue using the NucleoSpin Plant II kit (Macherey-Nagel, Denmark). DNA was extracted according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA containing the ACE-tRNA TTT sequences was midiprepped with the NucleoBond PC 20 miniprep kit (Macherey-Nagel). 16HBE14o-cells were diluted to 6 x 105 cells per mL with 1 mL added per well of a coated 6-well plate as previously described22 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the cells were collected from the plate and plasmid pools were extracted using the NucleoBond Xtra Midi Kit (Macherey-Nagel) according to the manufacturer’s recommendation.
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were extracted from mid-log yeast cultures grown 16 h at 20°C in YPD as described above using the Nucleospin RNA II kit (Macherey-Nagel) and were reverse transcribed with Superscript-II reverse transcriptase (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... De-crosslinking was performed by adding 20 µl Proteinase K (20 mg/ml, Macherey-Nagel, 740506) to the ligation mix (1.2 ml ...
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bioRxiv - Genetics 2021Quote: Genomic DNA from 5 males was extracted using the NucleoSpin Tissue XS kit (Macherey-Nagel, ref #740 901). To amplify the Rsp satDNA region ...
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bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... cells were scraped from plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel). The resulting plasmid DNA was used for yeast transformation.
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bioRxiv - Genetics 2022Quote: ... the cells were scraped from the plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated from confluent cells from one well of a 6-well plate with the NucleoSpin RNA isolation kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: We extracted DNA from the biomass pellets stored at −20°C using a NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Deutschland), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from six-day-old seedlings (approximately 20 seedlings per genotype) using the NucleoSpin RNA plant kit (Macherey-Nagel CO.). Subsequently ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in water ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL 1X TE buffer ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL water ...
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bioRxiv - Genetics 2019Quote: Total RNA from pools of 5 oocytes were extracted using the Nucleospin RNA XS kit according to the manufacturer’s protocol (Macherey-Nagel) and including a DNase1 treatment ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA was generated by isolating RNA from 5×106 HEK293T cells with the Nucleospin RNA isolation Mini kit (Macherey-Nagel)according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were extracted from positive colonies using 5 mL liquid cultures with the NucleoSpin Plasmid Purification kit (Macherey-Nagel; Ref.: 11932392) and sequenced (GATC ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and NucleoBond AXG 20 columns (Macherey-Nagel, Germany). Long-read sequencing was performed using a Flow Cell Wall Wash Kit (EXP-WSH004 ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl of Glutathione Sepharose 4B (Macherey-Nagel) (75% ...
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bioRxiv - Developmental Biology 2022Quote: gDNA from 6 male and 5 female pupae (sexed by eye) was extracted using the NucleoSpin Tissue kit (Macherey-Nagel, Düren, Germany), pooled by sex and used as template for subsequent PCRs ...
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bioRxiv - Genomics 2024Quote: ... each side of the Amicon filter was rinsed and incubated at 56°C for 1 hour (per side) in 200 µL Buffer T1 and 20 µL Proteinase K from the NucleoSpinl1J Tissue Kit (MACHEREY-NAGEL Inc., Allentown, PA, United States) and DNA extraction proceeded from step 3 of the kit ...
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bioRxiv - Molecular Biology 2019Quote: ... Starting from overnight grown liquid cultures of respective pAGM9121 constructs (prepared as described in section 5) plasmid mixtures were prepared using a NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, DE). Following the preparation ...
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bioRxiv - Microbiology 2020Quote: ... The final PCR product from the 5’ RACE procedure was excised from a 1% agarose gel and purified by column capture (Macherey-Nagel Nucleospin mini kit). The purified product was then Sanger sequenced (Eurofins Scientific ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from ACE-grown cells (DSMZ medium 461 with 5 mM ACE) with the NucleoSpin® Microbial DNA kit (Macherey-Nagel, Düren, Germany) according to the manual and using wide-bore tips ...
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bioRxiv - Systems Biology 2024Quote: ... Octadecyl C18 Empore disks were purchased from 3M and Polygoprep 300-20 C18 powder was purchased from Macherey-Nagel. Centrifugal filters were from Amicon ...
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bioRxiv - Biochemistry 2020Quote: ... Individual quenched timepoints were spotted PEI-TLC plates (Macherey-Nagel) and nucleotides were resolved in 1.5 KH2PO4 pH 3.5 buffer ...
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bioRxiv - Genetics 2021Quote: ... Vacuum the EconoSpin plate on the vacuum manifold (MACHEREY-NAGEL, Dueren, Germany) until no droplet drop down ...