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Citations for Tonbo Biosciences :
1 - 47 of 47 citations for IL 4RA CD124 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cells were subjected to Fc receptor blocking using Fc Shield (Tonbo Biosciences) for 10 minutes ...
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bioRxiv - Immunology 2020Quote: ... recombinant IL-4 and IL-5 (10 ng/mL; Tonbo Bioscience) cytokines ...
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bioRxiv - Microbiology 2023Quote: ... cells were stained with a Fc receptor block (Fc Shield, Tonbo Biosciences, Cat. No. 70-0161), a rabbit antibody against the MHV68 ORF4 protein (vRCA ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% penicillin-streptomycin-fungizone [PSF]) or basal media supplemented with 10 ng/mL of IL-1β (human recombinant; Tonbo Bioscience 218018U010) for 2 weeks ...
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bioRxiv - Cancer Biology 2023Quote: ... a Fc receptor blocker (Tonbo Biosciences 70-0161-M001 ...
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bioRxiv - Immunology 2021Quote: ... or IL-33 (Tonbo Biosciences). Negative control cells were treated with PBS while positive control cells were treated with Cell Stimulation Cocktail (eBioscience) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were immediately blocked for Fc (Tonbo Bioscience) and stained for B8R tetramer (AF488 ...
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bioRxiv - Immunology 2021Quote: ... and APC-IL-4 (Tonbo Bioscience) for 30 minutes at 4◦C ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were stained at 4°C in the dark with fixable live/dead dye diluted in PBS for 10 minutes followed by Fc-receptor blockade with Fc Shield (Tonbo Biosciences) for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD16/32 Fc block (2.4G2, Tonbo Biosciences). Measurement of mitochondrial content was performed using MitoTracker Green (Molecular Probes ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes were then subjected to FC block (Tonbo Bioscience) and stained with anti-CD3 (FITC ...
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bioRxiv - Immunology 2022Quote: ... and recombinant Mouse IL-15 (Tonbo Biosciences) were suspended and mixed in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... 10ng/mL IL-15 (Tonbo Biosciences, 218157U002), and 10ng/mL IL-7 (Tonbo Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Fc block (clone 93, BioLegend or clone 2.4G2, Tonbo Biosciences), and recombinant mouse TGF-β (5231 ...
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bioRxiv - Immunology 2020Quote: ... and IL-4 (10 ng/mL; Tonbo Bioscience), or TLR ligand CpG (2.5 μM ...
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bioRxiv - Microbiology 2021Quote: ... and IL-2-APC (Tonbo bioscience, 20-7021) on ice for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... and 10ng/mL IL-7 (Tonbo Biosciences, 218079U002). Stimulated PBMCs were electroporated using the Neon transfection system (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... cells were blocked with Fc receptor antibody (clone: 2.4G2, Tonbo Biosciences) and then stained with fluorochrome conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... 10 ng/mL recombinant mouse IL-4 (Tonbo Bioscience) plus 20 ng/mL recombinant human BAFF (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... and recombinant IL-4 cytokine (10 ng/mL; Tonbo Bioscience) with or without SCD inhibitor SSI-4 ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were blocked in αCD16/32 mouse Fc block (Tonbo Biosciences #70-0161) and stained for extracellular proteins using an antibody master mix made in buffer ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with 1 μg Fc Block (anti-CD16/32, Tonbo Biosciences) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: Staining panels for mouse included anti-Fc (anti-CD16/CD32) (Tonbo Biosciences, 2.4G2), anti-CD11b (Tonbo Biosciences ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... prior to Fc-block incubation (anti-mouse CD16/CD32, clone 2.4G2, Tonbo Biosciences). CD8+ T cells were purified by negative selection using biotinylated antibodies and magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... Cell staining was conducted in the presence of Fc Block (2.4G2, Tonbo Biosciences) at 4°C for 30 minutes for all surface markers except CXCR5-biotin which was stained at room temperature for 45 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in Fc block (1:100, Tonbo Bioscience, 70-0161-U500) followed by indicated antibodies and fixed with 1% PFA in flow cytometry buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... a Fc receptor blocker (Tonbo Biosciences 70-0161-M001, Tonbo Biosciences, San Diego, CA) was added for 10 minutes at 4°C to prevent non-specific binding of antibodies ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse CD16/32 antibody (2.4G2) used for Fc receptor blocking was obtained from Tonbo Biosciences. Unconjugated mouse and rat IgG antibodies were purchase from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Immunology 2024Quote: DAPI (#11034-56, Nacalai Tescue) and Fc block (clone 93, BioLegend or clone 2.4G2, Tonbo Biosciences) were added to the cells just before staining with specific antibodies against cell surface molecules ...
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bioRxiv - Immunology 2023Quote: ... IL-10 ELISA matched antibodies were obtained from Tonbo Biosciences (San Diego, CA, USA) and the ELISA was carried out according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cells were then incubated with Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 15-minute on ice ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in staining buffer containing Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 25-minute on ice ...
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bioRxiv - Neuroscience 2024Quote: Cells were then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) then washed with FACS buffer ...
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bioRxiv - Immunology 2024Quote: ... Anti-mouse CD16/CD32 Fc Block antibody purchased at a concentration of 2mg/mL (Tonbo Biosciences 40-0161-M001) was added at 0.5 uL per sample (in 100uL total volume per sample of block antibody diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... samples were washed with PBS pH7.2 (Fisher Cat#20012027) and incubated with Fc Block (Tonbo Biosciences Cat# 70-0161-U500) for 5 minutes on ice before viability staining (BD Biosciences Cat# 564406) ...
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bioRxiv - Developmental Biology 2023Quote: Lungs were plated at 1 × 106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-human CD4 (Tonbo Biosciences, 20-0048-T025), and sorted for live 7-AAD- CD3+ CD4+ T-lymphocytes using the BDFACS ARIA II with a 100 uM nozzle ...
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bioRxiv - Developmental Biology 2022Quote: Lung cells were plated at 1×106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Immunology 2024Quote: ... APCCy7 anti-human/mouse CD11b (clone M1/70, Tonbo Biosciences, 1:2000), PECy7 anti-mouse CD11c (clone N418 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 300 μL of 0.5% Triton X-100 (200 μL) + 2 μL of Fc Shield (anti-mouse CD16/CD32, Tonbo Biosciences, 70-0161-U500) + 100 μL of 5% BSA/PBS and incubated overnight at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... 0.025% NaN3) and Fcγ receptors were blocked with 1/200 diluted anti-mouse CD16/32 antibodies (Fc block) (Tonbo Biosciences, San Diego, CA, USA) for 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained using a dilution of 1:400 of anti-mouse CD16/CD32 (Fc Shield, #70-0161-U100; TONBO biosciences, San Diego, USA) in cRPMI for 15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... staining with APC Anti-Human CD3 (clone OKT3, 20-0037-T100, Tonbo Biosciences), monitoring GFP ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were resuspended in FACS buffer (2% FBS, 2mM EDTA, PBS) and then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) and washed with FACS buffer ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for conjugation assay: anti-human CD3-FITC (clone OKT3; Tonbo Biosciences# 35-0037-T100), anti-Flag-APC (clone L5 ...