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Citations for Tonbo Biosciences :
1 - 50 of 173 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were stimulated with 1 nM SIINFEKL (OT-I CD8+ T cells) or in wells coated with anti-CD3 (10 μg/ml; clone: 145-2C11; Tonbo Biosciences) and anti-CD28 (2 μg/ml ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were stimulated with plate-bound α-CD3 and soluble α-CD28 antibodies (both at 2 μg/ml; Tonbo Biosciences) for 72 hours ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were activated for 3 days with 10 ug/mL of plate-bound anti-CD3 (Tonbo Biosciences, clone UCHT1; #70-0038-U100) and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... These cells were immediately stimulated on anti-CD3-coated plates (coated overnight with 10 μg/ml anti-CD3 from Tonbo Biosciences) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were activated with plate-bound anti-CD3 (Tonbo Biosciences #40-0038) at a concentration of 10 µg/mL and anti-CD28 (Tonbo Biosciences #40-0289 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3 (Tonbo Biosciences, 25-0032), CD45 (Tonbo Biosciences ...
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bioRxiv - Immunology 2020Quote: ... αCD3 (40-0038, Tonbo Biosciences); αCD28 (70-0289 ...
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bioRxiv - Immunology 2020Quote: Cells were stained with the following antibodies for analysis CD3-FITC (35-0031, Tonbo Bioscience), CD8-BV711 (100748 ...
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bioRxiv - Immunology 2023Quote: Cells were stained with the following antibodies for analysis CD3-FITC (35-0031, Tonbo Bioscience), CD8-BV711 (100748 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then counted and stained with fluorescent dye–conjugated antibodies against CD3 (17A2; Tonbo Biosciences), CD4 (RM4-5 ...
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bioRxiv - Microbiology 2019Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3 coated plates [coated for 2 hours at 37°C with 20µg/mL anti-CD3 (UCHT1, Tonbo Biosciences)] in the presence of 5µg/mL soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2024Quote: ... the cell suspensions were stained using surface stain antibodies including Live/Dead Ghost Dye stain (Tonbo Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Lineage markers – CD3 (TONB55-0031-U100, Tonbo Biosciences), CD19 (15-1093-83 ...
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bioRxiv - Microbiology 2020Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3-coated plates [coated for 12 hours at 4°C with 20 µg/ml anti-CD3 antibody (UCHT1, Tonbo Biosciences)] in the presence of 5 µg/ml soluble anti-CD28 antibody (CD28.2 ...
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bioRxiv - Microbiology 2024Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3–coated plates coated for 2 hours at 37°C with anti-CD3 (20 mg/ml) (UCHT1; Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml ...
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bioRxiv - Immunology 2020Quote: Single cell suspensions were stained for surface antigens and a live/dead marker (Ghost Dye Violet 510, Tonbo Biosciences) in FACS buffer (PBS with 2% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: HLAC cells were stimulated for 3 days with plates coated with 10 µg/mL anti-CD3 (UCHT1, Tonbo Biosciences) and 10 µg/mL anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... These cells were immediately stimulated with plate bounded anti-CD3 (UCHT1, coated overnight with 10μg/ml αCD3 (UCHT1, Tonbo Biosciences)) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with anti-mouse CD3 violetFluor450 (Tonbo Biosciences; clone 17A2), CD4 APC (Tonbo Biosciences ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse CD3-FITC (clone 145-2C11; Tonbo Biosciences #35-0031-U500), anti-mouse CD19-violetFluor450 (clone 1D3 ...
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bioRxiv - Immunology 2022Quote: ... The purified CD4 T cells or unpurified splenocytes were labelled with 1μM of CFSE (Tonbo Biosciences, CA), by incubating in a water bath at 37°C for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... the ELISA kits to determine the levels of IFNγ and TNFα from house mouse (Mus muscuslus) (Tonbo Bioscience, San Diego, CA) were utilized to detect those cytokines in white-footed mice ...
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bioRxiv - Cancer Biology 2019Quote: ... staining with APC Anti-Human CD3 (clone OKT3, 20-0037-T100, Tonbo Biosciences), monitoring GFP ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were collected after 45 min incubation at 37 °C and 5 % CO2 and stained with antibodies to microglia cell surface markers (CD11b-PE-Cy7, 1:200, Biolegend, #101216; CD45-APC-Cy7, 1:200, Tonbo Biosciences, #25-0459-T100). XO4+ and XO4− microglia uptake of pHrodo-conjugated synaptosomes or pHrodo™ Green E ...
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bioRxiv - Immunology 2021Quote: ... Cells were surface stained in an additional 50 uL FACS containing 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13-0870-T100) and a cocktail of fluorescent anti-human antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... 1×106 PBMCs per well were stimulated for 48h in a CD3/CD28 (Tonbo Biosciences, 700037U100 and 70289U100 ...
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bioRxiv - Immunology 2023Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (Tonbo Biosciences Cat# 70-0031 (also OWL-A11492), RRID:AB_2621472 ...
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bioRxiv - Immunology 2019Quote: ... anti-CD3/anti-CD28 mAbs was used (OKT3, 70-0037-U100, CD28.2, 70-0289-U100, Tonbo Biosciences), cross-linked with anti-mouse mAb (405301 ...
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bioRxiv - Microbiology 2019Quote: ... anti-CD3 (17A2) and anti-CD45R/B220 (RA3) antibodies were obtained from Tonbo Biosciences (San Diego, CA); anti-CD45 (30-F11 ...
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bioRxiv - Immunology 2023Quote: ... IL-10 ELISA matched antibodies were obtained from Tonbo Biosciences (San Diego, CA, USA) and the ELISA was carried out according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected from 1.0 × 106 lung cells/mL media restimulated overnight with plate-bound anti-CD3 (adsorbed to wells at 1.0 μg/ml in PBS) and soluble anti-CD28 (1.0 μg/ml) (Tonbo Biosciences), as previously described (36 ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for conjugation assay: anti-human CD3-FITC (clone OKT3; Tonbo Biosciences# 35-0037-T100), anti-Flag-APC (clone L5 ...
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bioRxiv - Immunology 2020Quote: ... The PBMCs were cultured in three different ways: 1) Plate-bound anti-CD3 + anti-CD28 (clones OKT3 and CD28.2 at 2μg/ml each - Tonbo Biosciences) - plates were coated with Goat anti-mouse IgG (clone Poly4053 at 10μg/ml - BioLegend ...
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bioRxiv - Immunology 2021Quote: ... After the incubation a cocktail of fluorescently-labeled surface antibodies (2 µL of each: Ghost Dye Violet 510 Viability Dye, Tonbo Biosciences 13-0870-T100 ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with a 1:200 dilution of antibody for surface stains and A Live/Dead Ghost Dye Violet 510 (Tonbo Biosciences; 13-0870). Surface antibodies ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed with a FoxP3 Fix/Perm kit (eBioscience) or FoxP3/transcription factor staining buffer kit (Tonbo Biosciences). These kits were also used for permeabilization prior to staining with intracellular antibodies ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed and permeabilized (Transcription Factor Staining Buffer Kit, Tonbo Biosciences) and labeled with E16-APC for 20min at room temperature (56) ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed and permeabilized (Transcription Factor Staining Buffer Kit, Tonbo Biosciences) and labeled with E16-APC for 20min at room temperature (16) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were immediately fixed and permeabilized using a transcription factor staining kit according to the manufacturer protocol (Tonbo Biosciences Cat: TNB-0607-KIT). After fixation and permeabilization cells were spun down ...
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bioRxiv - Immunology 2020Quote: ... and cells were fixed and permeabilized for 30 min at room temperature using a FoxP3 / Transcription Factor Staining Buffer Kit (Tonbo Biosciences; cat. # TNB-0607-KIT). A panel of the following mAbs was used for the longitudinal phenotyping of innate immune cells in whole blood (500 μL) ...
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bioRxiv - Immunology 2022Quote: ... and cells were fixed and permeabilized for 30 min at room temperature using a FoxP3 / Transcription Factor Staining Buffer Kit (Tonbo Biosciences; cat. # TNB-0607-KIT). A panel of the following mAbs was used for the longitudinal phenotyping of innate immune cells in whole blood (500 μL) ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized with the Foxp3 Transcription factor staining Buffer kit according to manufacturer’s recommendations (Tonbo Biosciences), followed by intracellular staining with anti-Foxp3-PE (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with 1x Flow Cytometry Perm Buffer prepared as per manufacturer’s instructions (TNB-0607-KIT; Tonbo Biosciences) before staining with intracellular marker antibodies for 30 mins at 4°C in the dark (Table S2).
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bioRxiv - Immunology 2021Quote: ... Anti-mouse CD16/32 antibody (2.4G2) and Zombie UV Dead Cell Staining Kit were obtained from Tonbo Biosciences (San Diego, USA) and from BioLegend ...
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bioRxiv - Immunology 2024Quote: ... using the Foxp3/Transcription Factor Staining Buffer Kit (#TNB-0607-KIT, TONBO Biosciences) was performed ...
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bioRxiv - Cancer Biology 2022Quote: ... kit (Tonbo Biosciences, 20-1172), and EpCAM (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (TNB-0607-KIT, Tonbo Biosciences, San Diego, CA, USA), and stained with antibodies against intracellular proteins ...
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bioRxiv - Immunology 2020Quote: ... a transcription factor-staining kit (Tonbo Biosciences) was used ...
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bioRxiv - Immunology 2020Quote: ... Transcription Factor Staining Buffer Kit (Tonbo Biosciences) was used to fix and permeabilize cells before treating cells with Fc Block and subsequent intracellular staining ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were enumerated 5×106 cells stimulated with 1X of cell stimulation cocktail (Tonbo Biosciences, San Diego CA) for 5 hours ...