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Citations for Enzo Life Sciences :
1 - 41 of 41 citations for TMEM173 Human HEK293 Sumo His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... SUMO-1 and SUMO-2 were purchased from Enzo Life Sciences (Lausen, Switzerland). Reactions were performed for 2 hours at 30°C in in vitro buffer (20 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... and SUMO domain mutants of βC1 were incubated with SUMOylation cascade enzymes as per manufacturer’s instructions (SUMOylation kit, ENZO life sciences). HsSUMO1 was replaced with NbSUMO1 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins further purified by passing in a superdox 200 column were subjected to SUMO conjugation reactions with the help of commercially available in vitro SUMOylation assay kit (Enzo Life Sciences). Kit components comprising SUMO machinery enzymes and ATP were added to 500 nM of wild type SifA and SMUT SifA proteins separately in a 20 µl reaction mix along with provided controls at 37°C for 1 hour as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... His-tagged Hsf1 (Enzo Life Sciences: ADI-SPP-902-F). Hsf1 working concentrations were 250nM unless otherwise specified ...
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bioRxiv - Cell Biology 2020Quote: ... calreticulin (goat anti–human; Enzo Life Sciences), HA (mouse ...
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bioRxiv - Microbiology 2024Quote: ... Polyclonal human PKG antibodies (Enzo Life Sciences) were used for PfPKG detection (1:1000 or 1:2000 ...
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bioRxiv - Biophysics 2019Quote: The activity of recombinant human procaspase-3 and of recombinant human purified caspase-3 (Enzo Life Sciences, Lisboa, Portugal) was followed by monitoring the conversion of the fluorogenic substrate Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC ...
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bioRxiv - Cancer Biology 2021Quote: ... Clusterin (secretory form, human recombinant, Enzo Life Sciences) 0.5 μM ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA samples were assayed using AMPIGENE® qPCR Green Mix Hi-ROX with SYBR Green Dye (Enzo Life Sciences, Farmingdale, NY, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-1β (Enzo Life Sciences ref# ALX-522- 056) was used at 10 ng/mL final concentration in cell culture media.
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bioRxiv - Bioengineering 2024Quote: ... 100 ng/mL of recombinant human MEGACD40L (Enzo Life Sciences, Farmingdale ...
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bioRxiv - Microbiology 2020Quote: ... and human IL-1β (Enzo Life Sciences ref# ALX-522-056) were used at 100 ng/mL and 10 ng/mL final concentration respectively in cell culture media.
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bioRxiv - Immunology 2021Quote: ... Phase I: 100 ng/mL recombinant human MEGACD40L (Enzo Life Sciences), 1 mg/mL CpG oligodeoxynucleotide 2006 (Invitrogen) ...
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bioRxiv - Biophysics 2019Quote: ... HeLa cell lysate (human origin, Enzo Life Sciences, BML-SW8750, Switzerland) was incubated in the presence of 0.7 mM of biotinylated Fabs at 4 °C for 5 h ...
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bioRxiv - Immunology 2021Quote: ... anti-human CD95 Apo 1-1 was from Enzo Life Sciences. Flow Cytometry antibodies (for details see Flowcytometry ...
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bioRxiv - Biochemistry 2020Quote: ... from BPS Biosciences (San Diego, CA). SIRT5 (aa 37–310 with N-terminal His-tag, ≥95% purity; cat. #BML-SE555-0050) from Enzo Life Sciences (Farmingdale, NY). Purities were based on SDS-PAGE and Coomassie blue stain according to the supplier and all enzyme concentrations given were based on the stock concentrations determined by the supplier ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Cell Biology 2020Quote: ... 1.9 and 9.5 nM recombinant human visfatin (hrNAMPT(2)) (Enzo Life Sciences), 100 nM CVC (Med Chem Express) ...
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bioRxiv - Microbiology 2023Quote: ... 4 or 0.95 μM ARH1 or ARH3 (human, recombinant, Enzo Life Science) 5 in the presence of 10 mM Mg(OAc)2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Soluble recombinant human TRAIL was obtained from Enzo Life Sciences (Farmingdale, NY, USA). The pan-caspase inhibitor Z-VAD-FMK was purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Immunology 2022Quote: Rat anti-human GRP94 (ADI-SPA-850) was purchased from Enzo Life Sciences. The antihuman tapasin antibodies PaSta1(46 ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human Fas ligand (rhFasL) was purchased from Enzo Life Sciences (Farmingdale, NY). Recombinant IL-1β was purchased from PeproTech (Rocky Hill ...
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bioRxiv - Neuroscience 2022Quote: ... enhancer for ligand and recombinant human Fas-Fc were purchased from Enzo Life Sciences. z-IETD-fmk ...
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bioRxiv - Immunology 2022Quote: ... Dectin-2(murine)-IgG1 Fc (human) fusion protein (ENZO Life Sciences, Inc, Farmingdale, NY) or IgG1 Fc (human ...
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bioRxiv - Biophysics 2022Quote: ... soluble (human) (recombinant) set (ALX-850-014-KI02, Enzo Life Sciences Inc., Loerrach, Germany) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... raised against human polyubiquitin-B and polyubiquitin-C (1:100) (BML-PW8810, Enzo Life Sciences); Rabbit polyclonal antibody against protein disulfide isomerase (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated with rat anti human αSyn 15G7 primary antibodies (Enzo Life Sciences, 1:500) and rabbit anti-Iba1 (Wako ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human soluble TRAIL (ALX-201-073) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Human HGF (100-39 ...
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bioRxiv - Cell Biology 2020Quote: ... we performed an in-vitro plate-based fluorescence assay using recombinant human MKP-3 (Enzo Life Sciences) supplied in 20 mM Tris/HCl ...
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bioRxiv - Biochemistry 2021Quote: Sample preparation: Samples containing HTT-HAP40 complexes were digested using human Caspase6 (Enzo Life Sciences, Farmingdale, USA) by adding 200 U of the enzyme to the 20 μg of the protein ...
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bioRxiv - Molecular Biology 2020Quote: ... was purchased from Roche LifeScience (Penzberg, Germany) and rabbit anti-human PKG antibody from Enzo life sciences (New York, United States). A rabbit polyclonal antibody against MSP1-30 (Woehlbier et al. ...
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bioRxiv - Bioengineering 2022Quote: ... calpain substrate peptide was incubated with 15 nM human recombinant MMP-9 (Enzo Life Sciences, BML-SE360-0010) or 13.5 nM human α-thrombin (Haematologic Technologies ...
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bioRxiv - Physiology 2022Quote: ... Mouse and human serum OXT concentration was measured using an ELISA kit (Enzo Life Sciences, ADI-900-153A-0001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... or 300 ng/mL purified soluble human recombinant FAS ligand (SUPERFASLIGAND®, Enzo Life Sciences, Inc., Farmingdale, New York, USA) for 24 h ...
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bioRxiv - Biophysics 2019Quote: Fabs were generated from an antibody against the α4-subunit of the human 20S proteasome (Enzo Life Sciences, BML-PW8120, Switzerland), using a commercial kit (Thermo Fisher Scientific Inc. ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... soluble recombinant human CD40L was added at a concentration of 0.25 μg/ml (MEGACD40L® protein, ENZO Life Sciences, Farmingdale, NY). After 4 to 5 d stimulation ...
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bioRxiv - Biochemistry 2023Quote: ... Mutations were inserted using complementary nucleotides and Liposome-mediated transfections of 3x105 human HeLa cells were performed using DOTAP Liposomal Transfection Reagent (Alexis Biochemicals) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... and CL1-5 (shFUT4 and scramble control) human lung cancer cells were pre-stained with Cyto-IDTM long-term dyes (Enzo Life Sciences), and were injected into the tail vein of 6-week-old nude mice with 5*105 cells per mouse ...
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bioRxiv - Cancer Biology 2019Quote: ... and CL1-5 (shFUT4 and scramble control) human lung cancer cells were pre-stained with Cyto-ID™ long-term dyes (Enzo Life Sciences), and intracardially injected into the right ventricle of 6-week-old C57BL/6JNarl mice (5 mice per group ...