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Citations for Enzo Life Sciences :
1 - 50 of 146 citations for Recombinant Mouse Cxcl2 His & SUMO tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2020Quote: ... His-tagged Hsf1 (Enzo Life Sciences: ADI-SPP-902-F). Hsf1 working concentrations were 250nM unless otherwise specified ...
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bioRxiv - Cell Biology 2023Quote: ... SUMO-1 and SUMO-2 were purchased from Enzo Life Sciences (Lausen, Switzerland). Reactions were performed for 2 hours at 30°C in in vitro buffer (20 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biochemistry 2022Quote: ... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
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bioRxiv - Plant Biology 2020Quote: ... and SUMO domain mutants of βC1 were incubated with SUMOylation cascade enzymes as per manufacturer’s instructions (SUMOylation kit, ENZO life sciences). HsSUMO1 was replaced with NbSUMO1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Recombinant proteins and OA (Enzo Life Sciences) were diluted in PBS and centrifuged at 14,000 rpm at 4°C for 5 minutes before injection ...
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bioRxiv - Microbiology 2023Quote: ... Proteins further purified by passing in a superdox 200 column were subjected to SUMO conjugation reactions with the help of commercially available in vitro SUMOylation assay kit (Enzo Life Sciences). Kit components comprising SUMO machinery enzymes and ATP were added to 500 nM of wild type SifA and SMUT SifA proteins separately in a 20 µl reaction mix along with provided controls at 37°C for 1 hour as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: The activity of recombinant human procaspase-3 and of recombinant human purified caspase-3 (Enzo Life Sciences, Lisboa, Portugal) was followed by monitoring the conversion of the fluorogenic substrate Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC ...
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bioRxiv - Bioengineering 2020Quote: ... Recombinant proteases were purchased from Enzo Life Sciences, R&D Systems ...
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bioRxiv - Bioengineering 2020Quote: ... Recombinant proteases were purchased from Enzo Life Sciences and R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... Clusterin (secretory form, human recombinant, Enzo Life Sciences) 0.5 μM ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant >90% (ECB-LA636-100, Enzo Life Sciences)) were dissolved in 0.1 M PBS (18 μl ...
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bioRxiv - Bioengineering 2020Quote: ... Substrates were incubated with recombinant MMP12 (Enzo Life Sciences) at a final peptide concentration of 1 μM and a final enzyme concentration of 12.5 nM in a reaction volume of 30 μL in MMP-specific buffer (50 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... 100 ng/mL of recombinant human MEGACD40L (Enzo Life Sciences, Farmingdale ...
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bioRxiv - Immunology 2021Quote: ... Phase I: 100 ng/mL recombinant human MEGACD40L (Enzo Life Sciences), 1 mg/mL CpG oligodeoxynucleotide 2006 (Invitrogen) ...
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bioRxiv - Bioengineering 2020Quote: ... and S19-Z were incubated with recombinant MMP13 (Enzo Life Sciences); S2-Z ...
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bioRxiv - Biochemistry 2021Quote: HTT protein samples were mixed with recombinant Caspase6 (Enzo Life Sciences) in a ratio of 100 U caspase6 to 1 pmol of HTT in 20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... 1.9 and 9.5 nM recombinant human visfatin (hrNAMPT(2)) (Enzo Life Sciences), 100 nM CVC (Med Chem Express) ...
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bioRxiv - Cancer Biology 2022Quote: 1.5 μg of recombinant GST-AKT3 (BML-SE369-0005, Enzo Life Sciences), 0.1 μg of Recombinant Active TBK1 (T02-10G-05 ...
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bioRxiv - Microbiology 2023Quote: ... 4 or 0.95 μM ARH1 or ARH3 (human, recombinant, Enzo Life Science) 5 in the presence of 10 mM Mg(OAc)2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HDAC inhibition recombinant assay enzyme kit was purchased from Enzo Life Sciences and was performed according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... Soluble recombinant human TRAIL was obtained from Enzo Life Sciences (Farmingdale, NY, USA). The pan-caspase inhibitor Z-VAD-FMK was purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Neuroscience 2020Quote: ... 2 nM of recombinant catalytic domain of MT5-MMP (MT5cd, Enzo Life Science) was incubated with supernatants from GFP-transfected HEKswe at 37°C in activity buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: Recombinant catalytic domain of p300 was purchased from Enzo Life Sciences (BML-SE451). To remove endogenous acetylation from the recombinant protein ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human Fas ligand (rhFasL) was purchased from Enzo Life Sciences (Farmingdale, NY). Recombinant IL-1β was purchased from PeproTech (Rocky Hill ...
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bioRxiv - Cell Biology 2024Quote: Recombinant p66Shc was sumoylated with a commercially available sumoylation kit (Enzo Life Sciences). Sumoylated protein was resolved by SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... In vitro cleavage was assayed by adding 600 nM recombinant TACE (Enzo Life Sciences), using 10 μg of peptide substrate in a total volume of 25 μl ...
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bioRxiv - Neuroscience 2022Quote: ... enhancer for ligand and recombinant human Fas-Fc were purchased from Enzo Life Sciences. z-IETD-fmk ...
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bioRxiv - Neuroscience 2023Quote: ... lysates and precipitates were treated with recombinant deubiquitinase USP2 catalytic domain (Enzo Life Sciences) for 1h at 37°C at a ratio of 1 ...
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bioRxiv - Biophysics 2022Quote: ... soluble (human) (recombinant) set (ALX-850-014-KI02, Enzo Life Sciences Inc., Loerrach, Germany) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human soluble TRAIL (ALX-201-073) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Human HGF (100-39 ...
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bioRxiv - Cell Biology 2020Quote: ... we performed an in-vitro plate-based fluorescence assay using recombinant human MKP-3 (Enzo Life Sciences) supplied in 20 mM Tris/HCl ...
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bioRxiv - Biochemistry 2020Quote: ... a rabbit polyclonal raised against recombinant PARP1 (Enzo Life Sciences ALX-210-302-R100; 1:4000 dilution); anti-N-ter-PARP1 ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with 5μg of freshly isolated vesicles and recombinant protein HSC70 (Enzo Life Sciences, ADI-SPP-751) in MOPS buffer (10 mM 3-(N-morpholino ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant MMP-9 from the Matrix Metalloproteinase-9 Colorimetric Drug Discovery Kit (Enzo Life Sciences, New York, USA) was added to the supernatants ...
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bioRxiv - Bioengineering 2022Quote: ... calpain substrate peptide was incubated with 15 nM human recombinant MMP-9 (Enzo Life Sciences, BML-SE360-0010) or 13.5 nM human α-thrombin (Haematologic Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA samples were assayed using AMPIGENE® qPCR Green Mix Hi-ROX with SYBR Green Dye (Enzo Life Sciences, Farmingdale, NY, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... or 300 ng/mL purified soluble human recombinant FAS ligand (SUPERFASLIGAND®, Enzo Life Sciences, Inc., Farmingdale, New York, USA) for 24 h ...
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bioRxiv - Biochemistry 2020Quote: ... from BPS Biosciences (San Diego, CA). SIRT5 (aa 37–310 with N-terminal His-tag, ≥95% purity; cat. #BML-SE555-0050) from Enzo Life Sciences (Farmingdale, NY). Purities were based on SDS-PAGE and Coomassie blue stain according to the supplier and all enzyme concentrations given were based on the stock concentrations determined by the supplier ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ALX-522-013-C050) and its FLAG-tagged TNF-α ligand (cat. no. ALX-522-008-C050) were obtained from Enzo Life Sciences (San Diego, CA, USA). Binding inhibition assays were performed in a 96-well cell-free format similar to the one described before (53 ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... soluble recombinant human CD40L was added at a concentration of 0.25 μg/ml (MEGACD40L® protein, ENZO Life Sciences, Farmingdale, NY). After 4 to 5 d stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal anti-Bap31 (Alexis Biochemicals), rabbit polyclonal anti-Synoviolin (Bethyl laboratories Inc.) ...
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bioRxiv - Plant Biology 2020Quote: ... Mouse anti HSP73 (Enzo life sciences, Cat# ADI-SPA-818 ...
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bioRxiv - Microbiology 2022Quote: ... mouse FK2 (#PW8810, Enzo Life Sciences), Alexa-555-conjugated anti-rabbit antibody (#10082602 ...
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bioRxiv - Biochemistry 2023Quote: ... KDEL (mouse monoclonal, Enzo Life Sciences), Cox IV (rabbit polyclonal ...
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bioRxiv - Biophysics 2021Quote: ... and Bassoon-mouse (SAP7F407, Enzo Life Sciences). Tissue samples in dishes were washed with PBS 3 times for 10 mins each to remove the sucrose-methylcellulose layer and blocked with 5% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal anti-CLIMP63 (Enzo Life Sciences), rabbit polyclonal anti-Erlin1 (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Giantin-mouse monoclonal antibody (Enzo Life Sciences) was used at 1:1000 for immunofluorescence ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-poly ubiquitin FK1 (Enzo Life Sciences) 1:200 ...
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bioRxiv - Synthetic Biology 2021Quote: ... mouse anti-Hsp70 (Enzo Life Sciences, cat # C92F3A) to detect Hsp70 ...