Labshake search

1 - 44 of 44 citations for P selectin Human HEK 293 His since 2019

• Where Honey Bee Vitellogenin may Bind Zn²⁺-Ions

  Vilde Leipart, et al., bioRxiv - Biochemistry 2022

  Quote: ... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
• FANCD2-FANCI is a clamp stabilized on DNA by monoubiquitination during DNA repair

  Pablo Alcón, et al., bioRxiv - Molecular Biology 2019

  Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
• Increased processing of SINE B2 non coding RNAs unveils a novel type of transcriptome de-regulation underlying amyloid beta neuro-pathology

  Yubo Cheng, et al., bioRxiv - Genomics 2020

  Quote: ... His-tagged Hsf1 (Enzo Life Sciences: ADI-SPP-902-F). Hsf1 working concentrations were 250nM unless otherwise specified ...
• Nix induced mitochondrial fission, mitophagy, and myocyte insulin resistance are abrogated by PKA phosphorylation

  Simone Cristina da Silva Rosa, et al., bioRxiv - Cell Biology 2020

  Quote: ... The Kemptide substrate (Enzo Life Sciences; #P-107; LRRASLG) was used as a positive control in each assay ...
• Misoprostol Treatment Prevents Hypoxia-Induced Cardiac Dysfunction Through a 14-3-3 and PKA regulatory motif on Bnip3

  Matthew D. Martens, et al., bioRxiv - Cell Biology 2021

  Quote: ... The Kemptide substrate (Enzo Life Sciences; #P-107; LRRASLG) was used as a positive control in each assay ...
• Cardiac myosin binding protein-C palmitoylation is associated with increased myofilament affinity, reduced myofilament Ca²⁺ sensitivity and is increased in ischaemic heart failure

  Alice Main, et al., bioRxiv - Cell Biology 2022

  Quote: ... p-cMyBP-C (1:1000, pSer282, Enzo Life Sciences, ALX-215-057-R050), myosin heavy chain (1:200 ...
• TANGO1 membrane helices create a lipid diffusion barrier at curved membranes

  Ishier Raote, et al., bioRxiv - Cell Biology 2020

  Quote: ... calreticulin (goat anti–human; Enzo Life Sciences), HA (mouse ...
• Activation loop phosphorylation and cGMP saturation of PKG regulate egress of malaria parasites

  Konstantinos Koussis, et al., bioRxiv - Microbiology 2024

  Quote: ... Polyclonal human PKG antibodies (Enzo Life Sciences) were used for PfPKG detection (1:1000 or 1:2000 ...
• A simple linearization method unveils hidden enzymatic assay interferences

  Maria F. Pinto, et al., bioRxiv - Biophysics 2019

  Quote: The activity of recombinant human procaspase-3 and of recombinant human purified caspase-3 (Enzo Life Sciences, Lisboa, Portugal) was followed by monitoring the conversion of the fluorogenic substrate Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC ...
• A long non-coding RNA in the let-7 complex acting as a potent and specific death effector of cancer cells

  Tosca Birbaumer, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Clusterin (secretory form, human recombinant, Enzo Life Sciences) 0.5 μM ...
• TSG-6 in extracellular vesicles from canine mesenchymal stem/stromal is a major factor in relieving DSS-induced colitis

  Ju-Hyun An, et al., bioRxiv - Cell Biology 2019

  Quote: ... cDNA samples were assayed using AMPIGENE® qPCR Green Mix Hi-ROX with SYBR Green Dye (Enzo Life Sciences, Farmingdale, NY, USA), according to the manufacturer’s instructions ...
• Pneumococcus suppresses host inflammatory response through COMMD2 mediated NF-κB degradation by aggrephagy

  Michael G. Connor, et al., bioRxiv - Microbiology 2023

  Quote: ... Human IL-1β (Enzo Life Sciences ref# ALX-522- 056) was used at 10 ng/mL final concentration in cell culture media.
• Combining Cell-Intrinsic and -Extrinsic Resistance to HIV-1 By Engineering Hematopoietic Stem Cells for CCR5 Knockout and B Cell Secretion of Therapeutic Antibodies

  William N. Feist, et al., bioRxiv - Bioengineering 2024

  Quote: ... 100 ng/mL of recombinant human MEGACD40L (Enzo Life Sciences, Farmingdale ...
• HuD (ELAVL4) gain-of-function impairs neuromuscular junctions and induces apoptosis in in vitro and in vivo models of amyotrophic lateral sclerosis

  Beatrice Silvestri, et al., bioRxiv - Neuroscience 2024

  Quote: ... to single cells and replated in Nutristem P/S supplemented with 10 µM Y-27632 ROCK inhibitor (Enzo Life Sciences). The next day differentiation was induced in DMEM/F12 (Dulbecco’s Modified Eagle’s Medium/ Nutrient Mixture F-12 Ham ...
• The histone demethylase KDM6B fine-tunes the host response to Streptococcus pneumoniae

  Michael G. Connor, et al., bioRxiv - Microbiology 2020

  Quote: ... and human IL-1β (Enzo Life Sciences ref# ALX-522-056) were used at 100 ng/mL and 10 ng/mL final concentration respectively in cell culture media.
• Efficient methods for generation and expansion of, and gene delivery to Rhesus Macaque plasma B cells

  Shannon Kreuser, et al., bioRxiv - Immunology 2021

  Quote: ... Phase I: 100 ng/mL recombinant human MEGACD40L (Enzo Life Sciences), 1 mg/mL CpG oligodeoxynucleotide 2006 (Invitrogen) ...
• Microfluidic protein isolation and sample preparation for high resolution cryo-EM

  Claudio Schmidli, et al., bioRxiv - Biophysics 2019

  Quote: ... HeLa cell lysate (human origin, Enzo Life Sciences, BML-SW8750, Switzerland) was incubated in the presence of 0.7 mM of biotinylated Fabs at 4 °C for 5 h ...
• Cytotoxic efficiency of human CD8⁺ T cell memory subtypes

  Arne Knörck, et al., bioRxiv - Immunology 2021

  Quote: ... anti-human CD95 Apo 1-1 was from Enzo Life Sciences. Flow Cytometry antibodies (for details see Flowcytometry ...
• Mechanism-based inhibitors of SIRT2: structure–activity relationship, X-ray structures, target engagement, regulation of α-tubulin acetylation and inhibition of breast cancer cell migration

  Alexander L. Nielsen, et al., bioRxiv - Biochemistry 2020

  Quote: ... from BPS Biosciences (San Diego, CA). SIRT5 (aa 37–310 with N-terminal His-tag, ≥95% purity; cat. #BML-SE555-0050) from Enzo Life Sciences (Farmingdale, NY). Purities were based on SDS-PAGE and Coomassie blue stain according to the supplier and all enzyme concentrations given were based on the stock concentrations determined by the supplier ...
• An early Shh-H₂O₂ feedback loop controls the progression of the regenerative program during adult zebrafish fin regeneration

  Marion Thauvin, et al., bioRxiv - Developmental Biology 2021

  Quote: ... An NAD(P)H oxidase inhibitor (Nox-i, #BML-El395-0010) was purchased from Enzo Life Sciences (Inc. Farmingdale, NY, USA). Cyclopamine (HH-i) ...
• The effect of estradiol during the early stages of osteoclast differentiation is associated with the accumulation of phosphorylated p53 in mitochondria and the inhibition of mitochondrial metabolism

  Adriana Marques-Carvalho, et al., bioRxiv - Cell Biology 2023

  Quote: ... 0.1% CHAPS and 100 μM caspase substrate (Ac-DEVD-p-nitroanilide (pNA) (ALX-260-03, Enzo Life Science, Farmingdale, NY, USA) for caspase 3 or Ac-LEHD-pNA (ALX-260-081 ...
• Enriched environmental conditions modify the gut microbiome composition and fecal markers of inflammation in Parkinson’s disease

  Yogesh Singh, et al., bioRxiv - Neuroscience 2019

  Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
• Macrophages provide a transient muscle stem cell niche via NAMPT secretion

  Dhanushika Ratnayake, et al., bioRxiv - Cell Biology 2020

  Quote: ... 1.9 and 9.5 nM recombinant human visfatin (hrNAMPT(2)) (Enzo Life Sciences), 100 nM CVC (Med Chem Express) ...
• A viral ADP-ribosyltransferase attaches RNA chains to host proteins

  Maik Wolfram-Schauerte, et al., bioRxiv - Microbiology 2023

  Quote: ... 4 or 0.95 μM ARH1 or ARH3 (human, recombinant, Enzo Life Science) 5 in the presence of 10 mM Mg(OAc)2 ...
• Synergistic anticancer effect of plasma-activated infusion and salinomycin by targeting autophagy and mitochondrial morphology

  Takashi Ando, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Soluble recombinant human TRAIL was obtained from Enzo Life Sciences (Farmingdale, NY, USA). The pan-caspase inhibitor Z-VAD-FMK was purchased from Merck Millipore (Darmstadt ...
• SARS-CoV-2 accessory proteins ORF7a and ORF3a use distinct mechanisms to downregulate MHC-I surface expression

  Najla Arshad, et al., bioRxiv - Immunology 2022

  Quote: Rat anti-human GRP94 (ADI-SPA-850) was purchased from Enzo Life Sciences. The antihuman tapasin antibodies PaSta1(46 ...
• Pericyte-Like Cells Undergo Transcriptional Reprogramming and Distinct Functional Adaptations in Acute Lung Injury

  CF Hung, et al., bioRxiv - Cell Biology 2019

  Quote: Recombinant human Fas ligand (rhFasL) was purchased from Enzo Life Sciences (Farmingdale, NY). Recombinant IL-1β was purchased from PeproTech (Rocky Hill ...
• Differential effect of Fas activation on spinal muscular atrophy motoneuron death and induction of axonal growth

  Salim Benlefki, et al., bioRxiv - Neuroscience 2022

  Quote: ... enhancer for ligand and recombinant human Fas-Fc were purchased from Enzo Life Sciences. z-IETD-fmk ...
• The C-type Lectin Receptor Dectin-2 is a receptor for Aspergillus fumigatus galactomannan

  Jennifer L. Reedy, et al., bioRxiv - Immunology 2022

  Quote: ... Dectin-2(murine)-IgG1 Fc (human) fusion protein (ENZO Life Sciences, Inc, Farmingdale, NY) or IgG1 Fc (human ...
• A Minimal Model of CD95 Signal Initiation Revealed by Advanced Super-resolution and Multiparametric Fluorescence Microscopy

  Nina Bartels, et al., bioRxiv - Biophysics 2022

  Quote: ... soluble (human) (recombinant) set (ALX-850-014-KI02, Enzo Life Sciences Inc., Loerrach, Germany) was used ...
• HIV-1 Nef acts in synergy with APOL1-G1 to induce nephrocyte cell death in a new Drosophila model of HIV-related kidney diseases

  Jun-yi Zhu, et al., bioRxiv - Molecular Biology 2024

  Quote: ... raised against human polyubiquitin-B and polyubiquitin-C (1:100) (BML-PW8810, Enzo Life Sciences); Rabbit polyclonal antibody against protein disulfide isomerase (1:100 ...
• α-Synuclein strains influence multiple system atrophy via central and peripheral mechanisms

  T. Torre Murazabal, et al., bioRxiv - Neuroscience 2020

  Quote: ... Cells were incubated with rat anti human αSyn 15G7 primary antibodies (Enzo Life Sciences, 1:500) and rabbit anti-Iba1 (Wako ...
• Spatiotemporal regulation of the hepatocyte growth factor receptor MET activity by sorting nexins 1/2 in HCT116 colorectal cancer cells

  Laiyen Garcia Delgado, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Recombinant human soluble TRAIL (ALX-201-073) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Human HGF (100-39 ...
• Zn²⁺ influx activates ERK and Akt signaling pathways through a common mechanism

  Kelsie J. Anson, Giulia A. Corbet, Amy E. Palmer, bioRxiv - Cell Biology 2020

  Quote: ... we performed an in-vitro plate-based fluorescence assay using recombinant human MKP-3 (Enzo Life Sciences) supplied in 20 mM Tris/HCl ...
• HAP40 orchestrates huntingtin structure for differential interaction with polyglutamine expanded exon 1

  Rachel J Harding, et al., bioRxiv - Biochemistry 2021

  Quote: Sample preparation: Samples containing HTT-HAP40 complexes were digested using human Caspase6 (Enzo Life Sciences, Farmingdale, USA) by adding 200 U of the enzyme to the 20 μg of the protein ...
• Plasmodium falciparum guanylyl cyclase-alpha and the activity of its appended P4-ATPase domain are essential for cGMP synthesis and blood stage egress

  Stephanie D. Nofal, et al., bioRxiv - Molecular Biology 2020

  Quote: ... was purchased from Roche LifeScience (Penzberg, Germany) and rabbit anti-human PKG antibody from Enzo life sciences (New York, United States). A rabbit polyclonal antibody against MSP1-30 (Woehlbier et al. ...
• An Activity-Based Nanosensor for Minimally-Invasive Measurement of Protease Activity in Traumatic Brain Injury

  Julia A. Kudryashev, et al., bioRxiv - Bioengineering 2022

  Quote: ... calpain substrate peptide was incubated with 15 nM human recombinant MMP-9 (Enzo Life Sciences, BML-SE360-0010) or 13.5 nM human α-thrombin (Haematologic Technologies ...
• Control of lipolysis by a population of oxytocinergic sympathetic neurons

  Erwei Li, et al., bioRxiv - Physiology 2022

  Quote: ... Mouse and human serum OXT concentration was measured using an ELISA kit (Enzo Life Sciences, ADI-900-153A-0001) according to the manufacturer’s instructions ...
• ARID1A Mutations Protect Follicular Lymphoma from FAS-dependent Immune Surveillance by Reducing RUNX3/ETS1-Driven FAS-Expression

  Martina Antoniolli, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or 300 ng/mL purified soluble human recombinant FAS ligand (SUPERFASLIGAND®, Enzo Life Sciences, Inc., Farmingdale, New York, USA) for 24 h ...
• Microfluidic protein isolation and sample preparation for high resolution cryo-EM

  Claudio Schmidli, et al., bioRxiv - Biophysics 2019

  Quote: Fabs were generated from an antibody against the α4-subunit of the human 20S proteasome (Enzo Life Sciences, BML-PW8120, Switzerland), using a commercial kit (Thermo Fisher Scientific Inc. ...
• Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopes

  Tatiana M. Garcia-Bates, et al., bioRxiv - Immunology 2020

  Quote: ... soluble recombinant human CD40L was added at a concentration of 0.25 μg/ml (MEGACD40L® protein, ENZO Life Sciences, Farmingdale, NY). After 4 to 5 d stimulation ...
• hnRNP A1 induces aberrant CFTR exon 9 splicing via a newly discovered Exonic Splicing Silencer element

  Christelle Beaumont, et al., bioRxiv - Biochemistry 2023

  Quote: ... Mutations were inserted using complementary nucleotides and Liposome-mediated transfections of 3x105 human HeLa cells were performed using DOTAP Liposomal Transfection Reagent (Alexis Biochemicals) according to manufacturer instructions ...
• Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma

  Hsuan-Hsuan Lu, et al., bioRxiv - Cancer Biology 2019

  Quote: ... and CL1-5 (shFUT4 and scramble control) human lung cancer cells were pre-stained with Cyto-IDTM long-term dyes (Enzo Life Sciences), and were injected into the tail vein of 6-week-old nude mice with 5*105 cells per mouse ...
• Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma

  Hsuan-Hsuan Lu, et al., bioRxiv - Cancer Biology 2019

  Quote: ... and CL1-5 (shFUT4 and scramble control) human lung cancer cells were pre-stained with Cyto-ID™ long-term dyes (Enzo Life Sciences), and intracardially injected into the right ventricle of 6-week-old C57BL/6JNarl mice (5 mice per group ...