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Citations for Enzo Life Sciences :
1 - 49 of 49 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... 160-240 ng of biotin- and Green496-dUTP-labeled (Enzo Life Sciences) (2-colour ...
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bioRxiv - Microbiology 2019Quote: ... a biotin-labeled in-vitro transcription reaction was carried out using the cDNA template (Enzo Life Sciences, Farmingdale, NY). Fifteen micrograms of cRNA was fragmented for hybridization to Affymetrix Canine Genome 2.0 Array GeneChips (Santa Clara ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 µM of labeled NEMO protein was incubated with concentrations from 0-2.25 µM of M1-Ub8 (#BML-UW0805-0100, Enzo Life Sciences) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2020Quote: ... and biotin-conjugated isolectin B4 (Enzo Life Science, USA) using a magnetic field ...
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bioRxiv - Genomics 2023Quote: ... or Green496-dUTP-labeled (Enzo Life Sciences) and used as above.
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bioRxiv - Biophysics 2020Quote: ... and an anti-biotin monoclonal antibody (Hsc70; 1B5; Enzo Life Sciences, Plymouth, PA, USA).
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bioRxiv - Cell Biology 2021Quote: ... Probe specific for Tf L1 family was labeled with Red-dUTP (Enzo Life sciences) using a nick translation kit (Abbot) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purified plasmids were labeled with Cy3- or Cy5-dUTP (Enzo Life Sciences Inc., Farmingdale, NY, USA) using a nick translation method ...
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bioRxiv - Cell Biology 2022Quote: ... Then neurons were labeled with appropriate primary antibodies against Hsp47 (Enzo Life Sciences, catalog #: ADI-SPA-470-F) (1:100 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... purified 100 nM HA-tUI protein were mixing with 10 µM ubiquitin protein (Enzo life sciences) for 5 min at room temperature in blocking buffer before adding to the sample ...
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bioRxiv - Biophysics 2021Quote: ... double-Cysteine mutants generated on the C-less background of BmrCD were eluted following Ni-NTA purification and labeled with 20-fold molar excess of 1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate (Enzo Life Sciences) at room temperature in the dark over a 4 h period ...
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bioRxiv - Biophysics 2022Quote: ... single- and double-cysteine mutants were labeled with three rounds of 20-fold molar excess MTSSL (Enzo Life Sciences) per cysteine at room temperature and moved to ice overnight after four hours ...
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bioRxiv - Biophysics 2022Quote: Double-cysteine mutants were labeled with two rounds of 20-fold molar excess 1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate (Enzo Life Sciences) per cysteine on ice in the dark over a 4-h period ...
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bioRxiv - Biophysics 2019Quote: Double cysteine mutants were labeled with two rounds of 20-fold molar excess 1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate (Enzo Life Sciences) per cysteine at room temperature in the dark over a 4-hour period ...
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bioRxiv - Cell Biology 2021Quote: ... Single labeled grids were incubated with a primary rabbit anti-20S antibody (Enzo Life Sciences, catalog # BML-PW9355, lot # 05101719) at 1:200 dilution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Recombinant proteins and OA (Enzo Life Sciences) were diluted in PBS and centrifuged at 14,000 rpm at 4°C for 5 minutes before injection ...
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bioRxiv - Biochemistry 2019Quote: ... Addition of unlabeled UTP was reduced by 25% and an equal amount of fluorescently labeled UTP (fluorescein-12-UTP or cyanine-5-UTP, Enzo Life Sciences) was added in to the reaction mixture ...
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bioRxiv - Cell Biology 2023Quote: The isolation of protein aggregates and the assessment of protein aggregation was conducted using the Proteostat kit (Enzo Life Science, ENZ-51023), as previously published14 ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-ubiquitinylated proteins (Enzo Life Sciences, 1:100). The sections were probed in appropriate secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... ubiquitinylated proteins (BML-PW8810-0500, Enzo Life Science, 1/2500). The horseradish peroxidase-labelled secondary antibodies used were ...
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bioRxiv - Biochemistry 2021Quote: HTT protein samples were mixed with recombinant Caspase6 (Enzo Life Sciences) in a ratio of 100 U caspase6 to 1 pmol of HTT in 20 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-protein disulfide isomerase (PDI) (ADI-SPA-891, Enzo Life Sciences / StressGen ...
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bioRxiv - Neuroscience 2019Quote: ... anti-protein disulfide isomerase (PDI) (ADI-SPA-891, Enzo Life Sciences / StressGen ...
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bioRxiv - Microbiology 2020Quote: ... and the cytosolic control proteins DnaK (DnaK monoclonal antibody, Enzo Life Sciences).
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bioRxiv - Cell Biology 2023Quote: ... a potent protein synthesis inhibitor (Enzo Life Sciences, catalog #: ALX-380-269). Cells were then chased for the indicated time ...
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bioRxiv - Genetics 2020Quote: ... Protein aggregates were identified using mouse anti-polyubiquitin 1:1000 (Enzo Life Sciences). Three muscles from each individual fly were imaged as z-series and flattened into a single image as a max projection using confocal microscopy for a total of 27 muscles per genotype ...
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bioRxiv - Cell Biology 2021Quote: Protein aggregation was visualized using ProteoStat Aggresome Detection Reagent (Enzo Life Science, Farmingdale, NY), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Dectin-2(murine)-IgG1 Fc (human) fusion protein (ENZO Life Sciences, Inc, Farmingdale, NY) or IgG1 Fc (human ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 μM receptor-associated protein (MEG-Inh) (BML-SE552-0100, Enzo Life Sciences) for 16 h ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were incubated with 20 μL of prewashed p62 agarose (Enzo Life Sciences) or amylose resin (negative control ...
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bioRxiv - Neuroscience 2024Quote: ... coiled-coil containing protein kinase (ROCK) inhibitor (ROCKi; Enzo Life Sciences, LKT-Y1000-M005) to a new plate ...
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bioRxiv - Biochemistry 2020Quote: ... and K63-linked mono- and polyubiquitinylated proteins were from Enzo Life Sciences (Farmingdale, NY, USA) and the antibody against β-actin (MEF studies ...
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bioRxiv - Cancer Biology 2019Quote: ... with an anti-heat shock protein A9 (anti-GRP75 - ADI-SPS-826, Enzo Life Sciences).
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bioRxiv - Microbiology 2020Quote: Ubiquitylated proteins were isolated using the UbiQapture-Q kit (Enzo Life Sciences, Farmingdale, New York, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... protein aggregate accrual in the heart was measured using a commercially available kit (Proteostat; Enzo Life Sciences)(Laor et al. ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with 5μg of freshly isolated vesicles and recombinant protein HSC70 (Enzo Life Sciences, ADI-SPP-751) in MOPS buffer (10 mM 3-(N-morpholino ...
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bioRxiv - Microbiology 2021Quote: ... respectively, while monoclonal antibody specific to anti-protein disulfide isomerase (PDI, clone 1D3) was obtained from Enzo Life Sciences, Inc (C ...
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bioRxiv - Physiology 2022Quote: ... We purchased the mitogen-activated protein kinase (MAPK) and PKB inhibitor ML-9 from Enzo Life Sciences (Farmingdale, NY).
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bioRxiv - Genetics 2023Quote: ... Mouse monoclonal antibody anti-Mono- and poly-ubiquitinylated proteins (FK2) from Enzo Life Sciences (1:200 dilution was used), Alexa Fluor 488 anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... the protein was immunoprecipitated using the same antibody and detected with rabbit anti-SQSTM1 (Enzo Life Sciences, BML-PW9860). For HA-tagged proteins ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... soluble recombinant human CD40L was added at a concentration of 0.25 μg/ml (MEGACD40L® protein, ENZO Life Sciences, Farmingdale, NY). After 4 to 5 d stimulation ...
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bioRxiv - Neuroscience 2021Quote: ... The cAMP levels in samples containing equal protein concentrations were quantified using ELISA-based assay kit (#ADI-900-163, ENZO Life Sciences). The assay was done following the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... Cultures with B cells only were stimulated using soluble αIgM (10 ug/mL, clone DA4-4, Fisher) with soluble trimeric MegaCD40L protein (100 ng/mL, Enzo Life Sciences).
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bioRxiv - Neuroscience 2020Quote: ... Equal loading and transfer of proteins was verified with a reversible protein stain (Ponceaus S) and by re-probing membranes with a housekeeping protein calnexin (1:20,000, #ADI-SPA-860, Enzo Life Sciences, Farmingdale, NY, USA). Integrated band density of each protein sample was measured using Image Studio Lite software (LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2019Quote: ... HBECs were seeded on human placental collagen (HPC)-coated 6-well dishes and propagated in BEGM supplemented with the rho-associated protein kinase inhibitor Y-27632 (10 µM; Enzo Life Sciences, Farmingdale, NY), which enhances cell proliferation (16) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg protein were mixed with 80 μl reaction buffer (lysis buffer without Triton X-100) and 10 μl ubiquitin-AMC (Enzo Life Sciences, BML-SE211-0025) for a final concentration of 1 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... 2004) were used to detect proteins of interest with the following primary antibodies: PDK1 (Cat# ADI-KAP-PK112, Enzo Life Sciences, Plymouth Meeting, PA, USA), PDK2 (Cat# AP7039b ...
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bioRxiv - Neuroscience 2021Quote: ... O-GlcNAcylation of proteins in cell lysates was detected by western blot with anti-O-GlcNAc monoclonal antibody RL-2 (Enzo Life Sciences, ALX-804-11-R100) at 1:1000 dilution with detection by goat anti-mouse secondary at 1:10000 ...
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bioRxiv - Systems Biology 2023Quote: ... Specific proteasome activity was determined as the difference between the total activity of protein extracts and the remaining activity in the presence of 20 μΜ MG132 (Enzo Life Sciences, BML-PI102-0005). Fluorescence was measured by multiple reads for 60 min at 37°C by TECAN Kinetic Analysis (excitation 380 nm ...