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Citations for Enzo Life Sciences :
1 - 50 of 340 citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... purified 20S proteasome (Enzo Life Sciences, BML-PW8720) was used as a positive control ...
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bioRxiv - Molecular Biology 2020Quote: ... and mouse anti-mono- and polyubiquitinylated conjugates clone FK2 and rabbit anti-20S proteasome α7 subunit (Enzo Life Sciences Inc.). Rabbit anti-MKS1 has been described previously 51,52 ...
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bioRxiv - Immunology 2020Quote: ... 5μg of synthetic polypeptide were incubated with 500ng of the 20S constitutive proteasome (CP) (Enzo Life Sciences) in 20mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... Hsp90 and 20S Proteasome was done using primary antibodies: HSP70 (ENZO Life Sciences, ADI-SPA-812-F), HSP90 (Cell Signalling ...
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bioRxiv - Developmental Biology 2020Quote: ... a mouse monoclonal proteasome 20S core particle antibody that recognizes alpha 1,2,3,4,5,6,7 subunits (1:200, Enzo Life Sciences, Cat. No. BML-PW8195) and rabbit GFP antibody (1:500 ...
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bioRxiv - Biophysics 2019Quote: Fabs were generated from an antibody against the α4-subunit of the human 20S proteasome (Enzo Life Sciences, BML-PW8120, Switzerland), using a commercial kit (Thermo Fisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... NP40 1% and protease inhibitors [Thermo]) and immunoprecipitation was performed overnight at 4°C using Proteasome 20S core subunits polyclonal antibody (ENZO Life Sciences BML-PW8155) and IP buffer (137 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... 20S CP (Enzo Life Sciences Cat# PW8160) (1:1250) ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μM MG-132 (Enzo Life Sciences), 25 μM Cycloheximide (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-20S (Enzo life sciences; Rabbit; 1:2000; PW9355), anti-Mpk1 (Santa Cruz ...
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bioRxiv - Cancer Biology 2020Quote: ... or 20 nM LMB (Enzo Life Sciences, Brussels, Belgium). Cell viability and proliferation was assessed by (i ...
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bioRxiv - Biochemistry 2020Quote: ... anti-20S α-subunits (Enzo Life Sciences, MCP231 PW8195), anti-Hsp70 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... 10-20 µM clozapine N-oxide (Enzo Life Sciences) dissolved in either ACSF or saline was added to the bath solution ...
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bioRxiv - Immunology 2021Quote: ... anti-20s β2i (Enzo Life Science, BML-PW8350-0025), and anti-20s β1i (Enzo Life Science ...
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bioRxiv - Immunology 2021Quote: ... and anti-20s β1i (Enzo Life Science, BML-PW8840-0025). Antibodies were probed at the manufacturer’s recommended dilution overnight at 4°C before a secondary antibody conjugated to HRP was used for imaging ...
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bioRxiv - Developmental Biology 2020Quote: ... and 20E (Enzo Life Sciences; final concentration of 20 nM). The cultures were incubated at RT (except for EcR mutant flies ...
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bioRxiv - Cancer Biology 2023Quote: ... and 20 µM Hoechst 33342 (ENZ-52401, Enzo Life Sciences) for 30 minutes at 37 °C in a humidified incubator ...
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bioRxiv - Physiology 2023Quote: ... 20-40µM CGP-37157 (Enzo Life Sciences #BML-CM119-0005) was added from the beginning of the experiment to inhibit NCLX and evaluate NCLX-dependent mCa2+ efflux ...
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bioRxiv - Neuroscience 2020Quote: ... The proteasome inhibitor MG132 was used at 5 μM (Enzo Life Science, Lyon, France) and bafilomycin A1 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... Proteasome inhibition was achieved using 5 μM MG-132 for 16 hours (Enzo Life Sciences). Cell cycle synchronization in HT22 cells or patient fibroblasts was achieved by treatment with 250 ng/ml nocodazole (Sigma ...
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bioRxiv - Immunology 2021Quote: ... and the p38 inhibitor SB203580 (20 μM, Enzo Life Sciences, Farmingdale, NY) were added into HLMEC cultures 1 h before NP exposure ...
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bioRxiv - Developmental Biology 2024Quote: ... or N2B27-2i + 20 nM Rapamycin (Enzo Life Sciences, BML-A275-0005). The following day ...
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bioRxiv - Cell Biology 2019Quote: ... Islets were incubated in 20 nM Exendin-4 (Enzo Life Sciences ENZ-PRT11-0500), 100 μM IBMX (Enzo Life Sciences BLM-PD140-0200) ...
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bioRxiv - Microbiology 2020Quote: ... VLP-producing cells were treated with 20 μM Z-VAD-FMK (Enzo Life Sciences) 3 hours following VLP transfection.
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were incubated with 20 μL of prewashed p62 agarose (Enzo Life Sciences) or amylose resin (negative control ...
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bioRxiv - Cancer Biology 2023Quote: The MDM2 inhibitor nutlin-3a and the proteasome inhibitor MG-132 were both purchased from Enzo Life Sciences, dissolved in DMSO and used at final concentrations of 10 µM and 20 µM respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... resuspended in PBS and incubated with 20 nM DiOC6(3) (Enzo Life Sciences International Inc.) for 30 min at 37°C in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... Proteasome peptidase activities from the cell lysates were assayed using fluorogenic peptide substrate Suc-LLVY-AMC (Enzo Life Sciences). The measured activity was normalized against total protein concentration ...
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bioRxiv - Immunology 2021Quote: ... immunoblotting was performed using the following antibodies: anti-20s β5i (Enzo Life Science, BML-PW8845-0025), anti-20s β2i (Enzo Life Science ...
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bioRxiv - Cancer Biology 2019Quote: ... and resuspended with PBS followed by staining with 20 nM DiOC6(3) (Enzo Life Sciences International Inc.) for 30 min at 37°C in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... pH=7.6) as well as a standard curve of purified 26S Proteasome (0.5, 1 and 10 g/L; Enzo Life Sciences Inc., USA) were included in each assay ...
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bioRxiv - Biophysics 2021Quote: ... double-Cysteine mutants generated on the C-less background of BmrCD were eluted following Ni-NTA purification and labeled with 20-fold molar excess of 1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate (Enzo Life Sciences) at room temperature in the dark over a 4 h period ...
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bioRxiv - Biophysics 2022Quote: ... single- and double-cysteine mutants were labeled with three rounds of 20-fold molar excess MTSSL (Enzo Life Sciences) per cysteine at room temperature and moved to ice overnight after four hours ...
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bioRxiv - Biophysics 2022Quote: Double-cysteine mutants were labeled with two rounds of 20-fold molar excess 1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate (Enzo Life Sciences) per cysteine on ice in the dark over a 4-h period ...
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bioRxiv - Biophysics 2019Quote: Double cysteine mutants were labeled with two rounds of 20-fold molar excess 1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate (Enzo Life Sciences) per cysteine at room temperature in the dark over a 4-hour period ...
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bioRxiv - Cell Biology 2021Quote: ... Single labeled grids were incubated with a primary rabbit anti-20S antibody (Enzo Life Sciences, catalog # BML-PW9355, lot # 05101719) at 1:200 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... The transparent retina was incubated with the FITC-conjugated Isolectin-B4 (20 μg/mL, Enzo Life Science, Farmingdale, NY, USA). Using the Biologic CMM Analyzer software (French College) ...
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bioRxiv - Molecular Biology 2019Quote: ... FLAG purified from transfected HEK293T cells under high salt conditions (0.5 M NaCl) was used in a 20 µl reaction following manufacturer instructions (BML-UW9920-0001 ENZO Life Sciences) with UBCH5a as E2 enzyme.
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bioRxiv - Biochemistry 2020Quote: ... the pellet was washed with 15 mL of 0.125% (v/v) n-octyl POE (n-octylpolyoxyethylene) in 20 mM sodium phosphate buffer pH 7.4 (ALEXIS Biochemicals, Lausen, Switzerland), homogenized with a Potter-Elvehjem homogenizer ...
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bioRxiv - Immunology 2021Quote: ... at a concentration of 0.1 μg/ml in the presence of 20 μM of Ma-APK(Dnp) or control peptide BML-P127 (Enzo Life Sciences) in a final volume of 100 μl/well ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed and resuspended with PBS followed by staining with 20 nM 3,3’-dihexyloxacarbocyanine iodide (DiOC6(3)) (Enzo Life Sciences International Inc. ...
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bioRxiv - Plant Biology 2019Quote: ... 4-day-old light-grown seedlings were incubated with either 20 µM oryzalin (Supelco Analytical) for 3 h or 2 µM latrunculin B (Enzo Life Science) for 2 h before imaging.
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bioRxiv - Biochemistry 2022Quote: Affinity-mediated selection of the binding molecules against recombinant Sirt3 was done by incubating 20 nM of the DEL library with 50 µg of Sirt3 in 400 µl HDACT buffer (Enzo life sciences) containing either Carba-NAD,100 µM (Dalton pharma ...
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bioRxiv - Molecular Biology 2020Quote: Crude proteasomal fractions were prepared from cells 54 and incubated with the 20S fluorophore substrate Suc-LLVY-AMC (Enzo Life Sciences Inc.). Fluorescence of each proteasomal preparation was measured on a Mithras LB940 (Berthold Technologies Inc. ...
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bioRxiv - Neuroscience 2023Quote: Seizures were induced in 8- to 12-week-old Derl1f/f and Derl1NesCre mice by intraperitoneal injections of 20 mg/kg KA (BML-EA123, Enzo Life Sciences) dissolved in distilled H2O ...
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bioRxiv - Cell Biology 2023Quote: ... Pre-treatment with MG132 was accomplished using a 20 mg/mL stock solution of MG132 (Enzo Life Sciences: #BML-PI102; resuspended in DMSO) that was diluted in 5% ethanol to a 0.5 mg/mL working stock ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... sucrose or fat consumption was measured 20 min following the administration of clozapine-N-oxide (CNO; diluted in saline, 3 mg/kg, Enzo Life Sciences, NY).
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bioRxiv - Biophysics 2021Quote: ... The reaction was stopped by addition of 20 μl of 160 μM bpV(Phen) (Potassium bisperoxo(1,10-phenanthroline) oxovanadate (V) hydrate) (Enzo Life Sciences cat. no. SML0889-25MG). The fluorescence was monitored using a microplate reader (Envision ...
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bioRxiv - Systems Biology 2023Quote: ... Specific proteasome activity was determined as the difference between the total activity of protein extracts and the remaining activity in the presence of 20 μΜ MG132 (Enzo Life Sciences, BML-PI102-0005). Fluorescence was measured by multiple reads for 60 min at 37°C by TECAN Kinetic Analysis (excitation 380 nm ...