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1 - 50 of 252 citations for Human Urocortin 3 UCN3 CLIA Kit since 2019

• A simple linearization method unveils hidden enzymatic assay interferences

  Maria F. Pinto, et al., bioRxiv - Biophysics 2019

  Quote: The activity of recombinant human procaspase-3 and of recombinant human purified caspase-3 (Enzo Life Sciences, Lisboa, Portugal) was followed by monitoring the conversion of the fluorogenic substrate Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC ...
• Zn²⁺ influx activates ERK and Akt signaling pathways through a common mechanism

  Kelsie J. Anson, Giulia A. Corbet, Amy E. Palmer, bioRxiv - Cell Biology 2020

  Quote: ... we performed an in-vitro plate-based fluorescence assay using recombinant human MKP-3 (Enzo Life Sciences) supplied in 20 mM Tris/HCl ...
• Control of lipolysis by a population of oxytocinergic sympathetic neurons

  Erwei Li, et al., bioRxiv - Physiology 2022

  Quote: ... Mouse and human serum OXT concentration was measured using an ELISA kit (Enzo Life Sciences, ADI-900-153A-0001) according to the manufacturer’s instructions ...
• TANGO1 membrane helices create a lipid diffusion barrier at curved membranes

  Ishier Raote, et al., bioRxiv - Cell Biology 2020

  Quote: ... calreticulin (goat anti–human; Enzo Life Sciences), HA (mouse ...
• Activation loop phosphorylation and cGMP saturation of PKG regulate egress of malaria parasites

  Konstantinos Koussis, et al., bioRxiv - Microbiology 2024

  Quote: ... Polyclonal human PKG antibodies (Enzo Life Sciences) were used for PfPKG detection (1:1000 or 1:2000 ...
• A long non-coding RNA in the let-7 complex acting as a potent and specific death effector of cancer cells

  Tosca Birbaumer, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Clusterin (secretory form, human recombinant, Enzo Life Sciences) 0.5 μM ...
• lncRNA DIGIT and BRD3 protein form phase-separated condensates to regulate endoderm differentiation

  Kaveh Daneshvar, et al., bioRxiv - Molecular Biology 2019

  Quote: ... Cyanine 3-UTP (Enzo Life Sciences, ENZ-42505) was added to the reaction at the final concentration of 2.5 mM ...
• Pneumococcus suppresses host inflammatory response through COMMD2 mediated NF-κB degradation by aggrephagy

  Michael G. Connor, et al., bioRxiv - Microbiology 2023

  Quote: ... Human IL-1β (Enzo Life Sciences ref# ALX-522- 056) was used at 10 ng/mL final concentration in cell culture media.
• Combining Cell-Intrinsic and -Extrinsic Resistance to HIV-1 By Engineering Hematopoietic Stem Cells for CCR5 Knockout and B Cell Secretion of Therapeutic Antibodies

  William N. Feist, et al., bioRxiv - Bioengineering 2024

  Quote: ... 100 ng/mL of recombinant human MEGACD40L (Enzo Life Sciences, Farmingdale ...
• The histone demethylase KDM6B fine-tunes the host response to Streptococcus pneumoniae

  Michael G. Connor, et al., bioRxiv - Microbiology 2020

  Quote: ... and human IL-1β (Enzo Life Sciences ref# ALX-522-056) were used at 100 ng/mL and 10 ng/mL final concentration respectively in cell culture media.
• Efficient methods for generation and expansion of, and gene delivery to Rhesus Macaque plasma B cells

  Shannon Kreuser, et al., bioRxiv - Immunology 2021

  Quote: ... Phase I: 100 ng/mL recombinant human MEGACD40L (Enzo Life Sciences), 1 mg/mL CpG oligodeoxynucleotide 2006 (Invitrogen) ...
• Microfluidic protein isolation and sample preparation for high resolution cryo-EM

  Claudio Schmidli, et al., bioRxiv - Biophysics 2019

  Quote: ... HeLa cell lysate (human origin, Enzo Life Sciences, BML-SW8750, Switzerland) was incubated in the presence of 0.7 mM of biotinylated Fabs at 4 °C for 5 h ...
• Cytotoxic efficiency of human CD8⁺ T cell memory subtypes

  Arne Knörck, et al., bioRxiv - Immunology 2021

  Quote: ... anti-human CD95 Apo 1-1 was from Enzo Life Sciences. Flow Cytometry antibodies (for details see Flowcytometry ...
• Enriched environmental conditions modify the gut microbiome composition and fecal markers of inflammation in Parkinson’s disease

  Yogesh Singh, et al., bioRxiv - Neuroscience 2019

  Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
• Dynamic posttranslational modifications of cytoskeletal proteins unveil hot spots under nitroxidative stress

  Eva Griesser, et al., bioRxiv - Cell Biology 2021

  Quote: ... 3-morpholinosydnonimine (SIN-1) was purchased from Enzo Life Sciences GmbH (Lörrach ...
• Brunner Syndrome associated MAOA dysfunction in human induced dopaminergic neurons results in dysregulated NMDAR expression and increased network activity

  Y. Shi, et al., bioRxiv - Neuroscience 2019

  Quote: ... 0.5mM adenosine 3’,5’-cyclic monophosphate (cAMP, Enzo Life Science), 2ng/ml transforming growth factor beta 3 (TGFβ3 ...
• Macrophages provide a transient muscle stem cell niche via NAMPT secretion

  Dhanushika Ratnayake, et al., bioRxiv - Cell Biology 2020

  Quote: ... 1.9 and 9.5 nM recombinant human visfatin (hrNAMPT(2)) (Enzo Life Sciences), 100 nM CVC (Med Chem Express) ...
• A viral ADP-ribosyltransferase attaches RNA chains to host proteins

  Maik Wolfram-Schauerte, et al., bioRxiv - Microbiology 2023

  Quote: ... 4 or 0.95 μM ARH1 or ARH3 (human, recombinant, Enzo Life Science) 5 in the presence of 10 mM Mg(OAc)2 ...
• Synergistic anticancer effect of plasma-activated infusion and salinomycin by targeting autophagy and mitochondrial morphology

  Takashi Ando, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Soluble recombinant human TRAIL was obtained from Enzo Life Sciences (Farmingdale, NY, USA). The pan-caspase inhibitor Z-VAD-FMK was purchased from Merck Millipore (Darmstadt ...
• SARS-CoV-2 accessory proteins ORF7a and ORF3a use distinct mechanisms to downregulate MHC-I surface expression

  Najla Arshad, et al., bioRxiv - Immunology 2022

  Quote: Rat anti-human GRP94 (ADI-SPA-850) was purchased from Enzo Life Sciences. The antihuman tapasin antibodies PaSta1(46 ...
• Pericyte-Like Cells Undergo Transcriptional Reprogramming and Distinct Functional Adaptations in Acute Lung Injury

  CF Hung, et al., bioRxiv - Cell Biology 2019

  Quote: Recombinant human Fas ligand (rhFasL) was purchased from Enzo Life Sciences (Farmingdale, NY). Recombinant IL-1β was purchased from PeproTech (Rocky Hill ...
• The substrate quality of CK2 target sites has a determinant role on their function and evolution

  David Bradley, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... and anti-CENP-A (3-19, Enzo Life Sciences, 1 ug/ml) at 4°C in a humid chamber ...
• Differential effect of Fas activation on spinal muscular atrophy motoneuron death and induction of axonal growth

  Salim Benlefki, et al., bioRxiv - Neuroscience 2022

  Quote: ... enhancer for ligand and recombinant human Fas-Fc were purchased from Enzo Life Sciences. z-IETD-fmk ...
• The C-type Lectin Receptor Dectin-2 is a receptor for Aspergillus fumigatus galactomannan

  Jennifer L. Reedy, et al., bioRxiv - Immunology 2022

  Quote: ... Dectin-2(murine)-IgG1 Fc (human) fusion protein (ENZO Life Sciences, Inc, Farmingdale, NY) or IgG1 Fc (human ...
• A Minimal Model of CD95 Signal Initiation Revealed by Advanced Super-resolution and Multiparametric Fluorescence Microscopy

  Nina Bartels, et al., bioRxiv - Biophysics 2022

  Quote: ... soluble (human) (recombinant) set (ALX-850-014-KI02, Enzo Life Sciences Inc., Loerrach, Germany) was used ...
• HIV-1 Nef acts in synergy with APOL1-G1 to induce nephrocyte cell death in a new Drosophila model of HIV-related kidney diseases

  Jun-yi Zhu, et al., bioRxiv - Molecular Biology 2024

  Quote: ... raised against human polyubiquitin-B and polyubiquitin-C (1:100) (BML-PW8810, Enzo Life Sciences); Rabbit polyclonal antibody against protein disulfide isomerase (1:100 ...
• Epac1 regulates cellular SUMOylation by promoting the formation of SUMO-activating nuclear condensates

  Wenli Yang, et al., bioRxiv - Cell Biology 2022

  Quote: ... The blots were incubated with primary antibodies against SUMO2/3 (Enzo Life Sciences, Cat ...
• The effect of estradiol during the early stages of osteoclast differentiation is associated with the accumulation of phosphorylated p53 in mitochondria and the inhibition of mitochondrial metabolism

  Adriana Marques-Carvalho, et al., bioRxiv - Cell Biology 2023

  Quote: ... for caspase 3 or Ac-LEHD-pNA (ALX-260-081, Enzo Life Science) for caspase 9 ...
• Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutation

  Yeranuhi Hovhannisyan, et al., bioRxiv - Pathology 2023

  Quote: ... cultured for 3 days at which 1 µg/ml ouabain (Enzo Life Sciences) was added for clonal selection during 3 more days ...
• α-Synuclein strains influence multiple system atrophy via central and peripheral mechanisms

  T. Torre Murazabal, et al., bioRxiv - Neuroscience 2020

  Quote: ... Cells were incubated with rat anti human αSyn 15G7 primary antibodies (Enzo Life Sciences, 1:500) and rabbit anti-Iba1 (Wako ...
• Spatiotemporal regulation of the hepatocyte growth factor receptor MET activity by sorting nexins 1/2 in HCT116 colorectal cancer cells

  Laiyen Garcia Delgado, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Recombinant human soluble TRAIL (ALX-201-073) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Human HGF (100-39 ...
• HAP40 orchestrates huntingtin structure for differential interaction with polyglutamine expanded exon 1

  Rachel J Harding, et al., bioRxiv - Biochemistry 2021

  Quote: Sample preparation: Samples containing HTT-HAP40 complexes were digested using human Caspase6 (Enzo Life Sciences, Farmingdale, USA) by adding 200 U of the enzyme to the 20 μg of the protein ...
• Plasmodium falciparum guanylyl cyclase-alpha and the activity of its appended P4-ATPase domain are essential for cGMP synthesis and blood stage egress

  Stephanie D. Nofal, et al., bioRxiv - Molecular Biology 2020

  Quote: ... was purchased from Roche LifeScience (Penzberg, Germany) and rabbit anti-human PKG antibody from Enzo life sciences (New York, United States). A rabbit polyclonal antibody against MSP1-30 (Woehlbier et al. ...
• Application of non-invasive low-intensity pulsed electric field with thermal cycling-hyperthermia for synergistically enhanced anticancer effect of chlorogenic acid on PANC-1 cells

  Chueh-Hsuan Lu, et al., bioRxiv - Cancer Biology 2019

  Quote: ... resuspended in PBS and incubated with 20 nM DiOC6(3) (Enzo Life Sciences International Inc.) for 30 min at 37°C in the dark ...
• CENP-A overexpression drives distinct cell fates depending on p53 status

  Daniel Jeffery, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Primary antibodies: CENP-A (3-19) 1:300 (ADI-KAM-CC006-E Enzo Life Sciences), E-cadherin (24E10 ...
• An Activity-Based Nanosensor for Minimally-Invasive Measurement of Protease Activity in Traumatic Brain Injury

  Julia A. Kudryashev, et al., bioRxiv - Bioengineering 2022

  Quote: ... calpain substrate peptide was incubated with 15 nM human recombinant MMP-9 (Enzo Life Sciences, BML-SE360-0010) or 13.5 nM human α-thrombin (Haematologic Technologies ...
• Mitochondrial OPA1 deficiency causes reversible defects in adult neurogenesis-associated spatial memory in mice

  Trinovita Andraini, et al., bioRxiv - Neuroscience 2021

  Quote: ... 3-(2,4-Dichloro-5-methoxyphenyl)-2-sulfanyl-4(3H)-quinazolinone (BML-CM127-0050, Enzo Life Sciences) was injected at 20mg/kg (in 10% DMSO ...
• Blocking Orai1 constitutive activity inhibits B-cell cancer migration and synergistically acts with drugs to reduce B-CLL cell survival

  Julien Scaviner, et al., bioRxiv - Cancer Biology 2023

  Quote: ... activity of caspase-3 was performed by using Ac-DEVD-AFC substrate (Enzo Life Sciences, France). Briefly ...
• Identifying therapeutic drug targets for rare and common forms of short stature

  Karol Estrada, et al., bioRxiv - Genetics 2020

  Quote: ... cells were then incubated in DMEM containing 0.75 mM IBMX (3-isobutyl-1-methylxanthine) (Enzo life sciences) for 15 min ...
• Thermal cycling-hyperthermia in combination with polyphenols, epigallocatechin gallate and chlorogenic acid, exerts synergistic anticancer effect against human pancreatic cancer PANC-1 cells

  Chueh-Hsuan Lu, et al., bioRxiv - Cancer Biology 2019

  Quote: ... and resuspended with PBS followed by staining with 20 nM DiOC6(3) (Enzo Life Sciences International Inc.) for 30 min at 37°C in the dark ...
• ERK1/2 signalling dynamics promote neural differentiation by regulating the polycomb repressive complex

  Claudia I. Semprich, et al., bioRxiv - Developmental Biology 2019

  Quote: ... and one half the litter exposed MEK inhibitor (PD184532, MEKi, 3 μM final concentration, Enzo Life Sciences) and the other half exposed to vehicle control DMSO (equal volume ...
• ARID1A Mutations Protect Follicular Lymphoma from FAS-dependent Immune Surveillance by Reducing RUNX3/ETS1-Driven FAS-Expression

  Martina Antoniolli, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or 300 ng/mL purified soluble human recombinant FAS ligand (SUPERFASLIGAND®, Enzo Life Sciences, Inc., Farmingdale, New York, USA) for 24 h ...
• Thermal cycling protects SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury through stress response mechanisms involving Akt pathway

  Wei-Ting Chen, et al., bioRxiv - Neuroscience 2020

  Quote: The MMP was determined by flow cytometry using 3,3'-dihexyloxacarbocyanine iodide (DiOC6(3)) (Enzo Life Sciences International Inc.). DiOC6(3 ...
• Where Honey Bee Vitellogenin may Bind Zn²⁺-Ions

  Vilde Leipart, et al., bioRxiv - Biochemistry 2022

  Quote: ... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
• Microfluidic protein isolation and sample preparation for high resolution cryo-EM

  Claudio Schmidli, et al., bioRxiv - Biophysics 2019

  Quote: Fabs were generated from an antibody against the α4-subunit of the human 20S proteasome (Enzo Life Sciences, BML-PW8120, Switzerland), using a commercial kit (Thermo Fisher Scientific Inc. ...
• Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopes

  Tatiana M. Garcia-Bates, et al., bioRxiv - Immunology 2020

  Quote: ... soluble recombinant human CD40L was added at a concentration of 0.25 μg/ml (MEGACD40L® protein, ENZO Life Sciences, Farmingdale, NY). After 4 to 5 d stimulation ...
• hnRNP A1 induces aberrant CFTR exon 9 splicing via a newly discovered Exonic Splicing Silencer element

  Christelle Beaumont, et al., bioRxiv - Biochemistry 2023

  Quote: ... Mutations were inserted using complementary nucleotides and Liposome-mediated transfections of 3x105 human HeLa cells were performed using DOTAP Liposomal Transfection Reagent (Alexis Biochemicals) according to manufacturer instructions ...
• Cryo-EM structure of an elusive pre-transport intermediate of the multidrug ABC transporter BmrCD reveals modes of asymmetric drug binding

  Tarjani M. Thaker, et al., bioRxiv - Biophysics 2021

  Quote: ... double-Cysteine mutants generated on the C-less background of BmrCD were eluted following Ni-NTA purification and labeled with 20-fold molar excess of 1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate (Enzo Life Sciences) at room temperature in the dark over a 4 h period ...
• Proton-driven alternating access in a spinster transporter, an emerging family of broad-specificity efflux pumps

  Reza Dastvan, et al., bioRxiv - Biophysics 2022

  Quote: Double-cysteine mutants were labeled with two rounds of 20-fold molar excess 1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate (Enzo Life Sciences) per cysteine on ice in the dark over a 4-h period ...
• Sequence and Structural Determinants of Ligand-dependent Alternating Access of a MATE Transporter

  Kevin L. Jagessar, et al., bioRxiv - Biophysics 2019

  Quote: Double cysteine mutants were labeled with two rounds of 20-fold molar excess 1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate (Enzo Life Sciences) per cysteine at room temperature in the dark over a 4-hour period ...