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Citations for Bio-Rad :
1 - 50 of 932 citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 7 μL RNase-free water and 10 μL iQ SYBR○R Green Supermix (Bio-Rad, USA). The standard curves were constructed from a series of 10-fold dilutions of a plasmid DNA obtained by TOPO TA cloning (ThermoFisher ...
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bioRxiv - Synthetic Biology 2022Quote: ... Tris Buffer RNase-free (Bio-Rad) after first exchanging the column buffer to water according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... RT+ and RT-reverse transcription reactions were diluted 67-fold in RNase/DNase free water and RT-qPCR was performed on the CFX96 Real Time System (BioRad) using GoTaq qPCR Master Mix (Promega) ...
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bioRxiv - Genomics 2022Quote: ... Tris Buffer (RNase-free) (Bio-Rad #7326250). Nascent RNA was purified by binding streptavidin beads (NEB S1421S ...
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bioRxiv - Molecular Biology 2021Quote: ... The converted DNA was eluted in DNase/RNase-free water and examined by PCR using SYBR Green PCR Master Mix (Bio-Rad) and primers listed in Supplementary Table S2 ...
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bioRxiv - Plant Biology 2020Quote: ... RNase-free Bio-Spin columns P-30 (Bio-Rad) were used for radiolabeled RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNA was purified using p-30 RNase-free columns (BioRad), and treated with RQ1 RNase-free DNase (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by passing through a calibrated RNase-free P-30 column (Bio-Rad). After 3’ RNA adaptor ligation ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was then purified through a p-30 RNAse-free spin column (BioRad), according to the manufacturer’s instructions and treated with 6.7 μl of DNase buffer and 10 μl of RQ1 RNase-free DNase (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... Unincorporated biotin-NTPs were removed with RNase free P-30 columns (7326223; Bio-Rad), and three cycles of magnetic MyOne Streptavidin C1 bead purification (65002 ...
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bioRxiv - Molecular Biology 2020Quote: ... and later purified by running through RNase-free P-30 column (Bio-Rad, Hercules, CA). PNK was used to dephosphorylate RNA for 2 hours (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: Micro Bio-Spin™ RNase free P-30 Gel Columns (Bio-Rad, cat. no. 7326250) (optional ...
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bioRxiv - Immunology 2020Quote: ... which were sorted into DNase and RNase free 96-well PCR plates (Bio-Rad, USA) at a single cell level by FACSAria III (BD Biosciences ...
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bioRxiv - Developmental Biology 2020Quote: ... Upon removal of genomic DNA (TURBO DNA-free™ Kit or RNase-free DNase Set) cDNA was synthesized using the iScript™ cDNA Synthesis Kit (Bio-Rad). PCR reactions were run in triplicate with 6.25-12.5 ng cDNA per reaction using the CFX96 Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Immunology 2020Quote: ... and single-cell sorted into 96-well PCR plates (free of DNase and RNase, Bio-Rad). The Plates were stored at −80 °C until BCR Cloning ...
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bioRxiv - Microbiology 2024Quote: ... Membrane was washed with nuclease-free water and imaged on a ChemiDoc MP Imaging system (BioRad). Denaturing polyacrylamide gels were made using the SequaGel UreaGel 29:1 Denaturing Gel System (EC-829) ...
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bioRxiv - Developmental Biology 2021Quote: ... The probes were cleaned using Micro Bio-SpinTM P-30 Gel Columns with RNase-free Tris Buffer (BioRad).
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bioRxiv - Neuroscience 2024Quote: ... Generated probes were purified using Micro Bio-SpinTM P-30 Gel Columns with RNase-free Tris Buffer (BioRad) according to the manufacturer’s protocol and stored at −80°C ...
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bioRxiv - Biochemistry 2021Quote: ... Unincorporated nucleotides were removed from the aqueous phase by using a P-30 Microspin column (RNase Free; Bio-Rad), and the oligonucleotide concentration was measured by using a Qubit ssDNA assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted with nuclease-free water and subsequently analyzed by qPCR using iTaq Universal SYBR Green Supermix (BioRad) on a Roche LightCycler 96 instrument (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA template (up to 8 μL) and nuclease-free water were loaded into a disposable droplet generator cartridge (Bio-Rad). After adding 70 μL of droplet generation oil (Bio-Rad) ...
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bioRxiv - Bioengineering 2020Quote: 50 μL of PCR mastermix solution in Nuclease-free ultrapure water contained 60 mM Tris-HCl Buffer pH 8.8 (Bio-Rad), 200 μM dNTPs ...
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bioRxiv - Bioengineering 2020Quote: ... 50 μL of PCR mix solution in nuclease-free water contained 60 mM Tris-HCl Buffer pH 8.8 (Bio-Rad), 200 μM dNTPs ...
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bioRxiv - Systems Biology 2020Quote: ... cDNA was diluted in nuclease-free water and quantified using SsoAdvanced Universal SYBR Green Supermix on a CFX Connect Real-Time System (BioRad) with the following amplification scheme ...
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bioRxiv - Bioengineering 2021Quote: ... The RNA concentration was quantified with a nanodrop and the samples concentration were evened with nucleoside-free water (Bio-Rad). To synthesize cDNA from RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted with nuclease-free water and subsequently analyzed by qPCR using iTaq Universal SYBR Green Supermix (BioRad) on a Roche LightCycler 96 instrument (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted with nuclease-free water and subsequently analyzed by qPCR using iTaq Universal SYBR Green Supermix (BioRad) on a Roche LightCycler 96 instrument (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted with nuclease-free water and subsequently analyzed by qPCR using iTaq Universal SYBR Green Supermix (BioRad) on a Roche LightCycler 96 instrument (Roche) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... aliquots constituting 250 ng of RNA were diluted with nuclease-free water to a volume of 15 µL and processed using the iScriptTM cDNA Synthesis Kit (BioRad Laboratories Inc. ...
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bioRxiv - Immunology 2022Quote: ... and RBD-specified single memory B cells were gated by IgG+RBD+ followed by single-cell sorting into 96-well PCR plates (free of DNase and RNase, Bio-Rad) containing 10μl lysis buffer (1X reverse transcriptase reaction buffer containing RNAse inhibitor) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was diluted 1:20 in RNase-free H2O and 3 µl dilution were used for qRT-PCR analysis using iTaq Universal SYBR Green (Bio-Rad) according to manufacturer’s recommendation ...
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bioRxiv - Plant Biology 2019Quote: ... qPCR analyses were performed with cDNAs diluted 50-fold with nuclease-free water using iTag Universal SYBR Green Supermix (Bio-Rad) or Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent ...
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bioRxiv - Genetics 2020Quote: ... twice in 200 μl nuclease free water and once in 100μl 1x SSC (150 mM NaCl, 15mM sodium citrate, pH 7.0, BIO-RAD Laboratories, Inc.) and finally resuspended in 50 μl 1X SSC ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was diluted 1:4 in nuclease free water and each cDNA was combined with segment specific primers (Supplementary Table 2) and Precision Melt Supermix (Bio-Rad) and analyzed by qPCR in a CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:4 with nuclease-free water and used as template (4 µL per reaction) for segment-specific qPCR using SsoFast EvaGreen Supermix (Bio-Rad) in 10 µl reactions ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was diluted 1:4 with nuclease-free water and used as template (4 uL/reaction) for segment-specific qPCR using SsoFast EvaGreen Supermix (Bio-Rad) in 10 uL reactions ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-amplified cDNA was diluted at least 5-fold with nuclease-free water and mixed with SsoFast EvaGreen with Low ROX (BioRad #1725211) and chip-specific DNA Sample Reagents before loading into primed Flex Six or 96.96 Dynamic Array chips (Fluidigm #100-6308 ...
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bioRxiv - Microbiology 2023Quote: ... 1 µl of a 1:10 dilution in nuclease-free water of the cDNA samples was mixed with 5 µl of SsoAdvanced Univ SYBR Green Supermix (Bio-Rad) and 0.8 µl of 5 µM forward and reverse primers (Table S10) ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was diluted 1:10 in nuclease free water prior to qPCR analysis using a CFX384 real-time PCR system (Bio-Rad, USA). Reaction mixtures containing 5 μl PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: RNA was diluted in nuclease-free water and combined with the reagents from the One Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, USA) and eGFP primer/probe set with final primer concentrations of 900 nM and probe concentrations of 250 nM to measure transgene expression ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR-fragments were gel-purified using Micro Bio-Spin P-30 Gel columns Tris Buffer (RNase-free)(Bio-Rad, Hercules, CA, USA Cat#7326250) and subsequently ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... molecular grade water (BioRad) and forward and reverse primers were added to 96-well plate ...
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bioRxiv - Neuroscience 2021Quote: ... diluted 1:1 with nuclease-free water and used as template for digital droplet PCR (ddPCR) using ddPCR Supermix for Probes (No dUTP) (Bio-Rad, Hercules, CA, USA) and a custom TaqMan SNP Genotyping Assay (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... Digested DNA was then diluted 1:1 with nuclease-free water and used for template for digital droplet PCR (ddPCR) using ddPCR Supermix for Probes (No dUTP) (Bio-Rad, Hercules, CA, USA) and custom TaqMan SNP Genotyping Assays (Life Technologies ...
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bioRxiv - Genetics 2020Quote: ... Digested DNA was then diluted 1:1 with nuclease-free water and used for template for digital droplet PCR (ddPCR) using ddPCR Supermix for Probes (No dUTP) (Bio-Rad, Hercules, CA, USA) and a custom TaqMan SNP Genotyping Assay (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Each 20 μL qPCR reaction was prepared in UV-treated UltraPure nuclease-free water containing 10 μL of EvaGreen® Supermix (Bio-Rad Laboratories, Hercules, CA), 0.5 μL of each primer (forward and reverse ...
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bioRxiv - Cancer Biology 2021Quote: ... Stain free technology (Biorad) was used for total protein detection in Figure 2E.
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bioRxiv - Physiology 2022Quote: ... and deionized water for gel electrophoresis (Bio-Rad). Samples were heated to 95°C for 5 min on a heating block (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... using Stain-Free (Bio-Rad) or Oriole (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... The liquid portion was sampled and analyzed against calibration standards and using HPLC Waters Alliance system e2695 (Waters Co., Milford, MA) equipped with an HPX-87H column (Bio-Rad Aminex® ...