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Citations for Bio-Rad :
1 - 50 of 4128 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The planar electrodes were commercial platinum-coated electrotransfer anodes (Bio-Rad Criterion anode plates) with plastic housings modified to permit close proximity of the electrode surfaces ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were blocked in PBS containing 5% goat serum (Bio-Rad Antibodies) and 0.1% saponin (SigmaAldrich ...
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bioRxiv - Plant Biology 2022Quote: ... Effector expression was quantified on the CFX96 real-time PCR detection system (Bio-Rad). Gene expression was calculated using the ΔCT method and normalized against the GlnA housekeeping gene ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of the different effectors in the infected material was evaluated using RT-qPCR SYBR Green (Bio-Rad). The samples were heated to 95°C for 2min ...
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bioRxiv - Plant Biology 2020Quote: ... and the proUbi-LUC reference construct (Bruce et al., 1989) with or without ARK effector constructs by using the PDS-1000He particle delivery system (Bio-Rad, Hercules, CA). The bombarded cells were incubated in a medium with or without ABA and were used for GUS and LUC assays (Bruce et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Native protein extracts from E.coli or Synechococcus ΔCS were incubated in IMAC HEPES binding buffer supplemented with potential effector molecules and Ni-charged iminodiacetic acid Profinity™ IMAC Resin (Bio-Rad, USA) on a low speed rotator for 1 h at 23°C ...
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bioRxiv - Plant Biology 2020Quote: ... were performed in biological triplicates with two technical repeats on the obtained cDNAs using a CFX96 Real-Time PCR Detection system (BioRad). Primer efficiencies and optimal primer concentrations were determined experimentally ...
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bioRxiv - Plant Biology 2020Quote: ... were performed in biological triplicates with two technical repeats on the obtained cDNAs using a CFX96 Real-Time PCR Detection system (BioRad). Primer efficiencies and optimal primer concentrations were determined experimentally ...
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bioRxiv - Microbiology 2023Quote: HIV-1/N and M strains were quantified by targeting the long terminal repeat (LTR) region using GENERIC HIV Charge virale kit (Biocentric) performed on CFX96 Deep-Well (Biorad) with the followed program RT 10 min at 50°C and 5 min at 95°C ...
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bioRxiv - Immunology 2022Quote: ... To each well 50 µL of 2 µg/mL of biotin-conjugated mouse anti-bovine IFN-γ mAb (CC302, Bio-Rad Antibodies) diluted in PBS was added and plates incubated for 2 hours at RT ...
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bioRxiv - Zoology 2020Quote: ... Telomeric repeats of sequence TTAGG were separated from restricted DNA with Pulsed Field Gel Electrophoresis (CHEF-DR II system by Bio-Rad) in a 0.8% agarose gel.
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bioRxiv - Immunology 2020Quote: ... absolute quantification of the HIV Long Terminal Repeat (LTR) was performed in a duplex-digital droplet PCR reaction using the Raindrop System (Raindrop Technologies / Bio-Rad). The Raindrop Source instrument was used to generate uniform aqueous droplets (5 picoliter ...
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bioRxiv - Physiology 2024Quote: ... Primary antibodies were diluted in TBS-T containing 5% dry nonfat milk (Biorad). Membranes were incubated overnight at 4 °C with primary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were diluted in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The bound antibodies were detected using luminol containing reagent Clarity Western ECL Substrate (BioRad) and visualized using ChemiDoc imager (BioRad).
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bioRxiv - Developmental Biology 2020Quote: ... secondary antibody diluted in TBST containing 0.5% BSA with streptavidin-HRP (Bio-Rad, 1:10,000) for 1 hour at room temperature ...
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bioRxiv - Genomics 2023Quote: ... and anti-F4/80-biotin (clone A3-1, Bio-rad, MCA497BT) antibodies for 40 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted 1:1000 in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies at 1:10,000 dilution (Bio-Rad #172-1011 or 172-1019 ...
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bioRxiv - Genetics 2019Quote: ... Migration of biotin-labeled probes was detected in the ChemiDoc MP Imager (BioRad) using streptavidin-horseradish peroxidase conjugates that bind to biotin and chemiluminescent substrate according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Nuclear and cytosolic fractions of iPSCs-differentiated cells were acquired using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific #78833 ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Protein samples were loaded on 4-15% polyacrylamide gels (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: ... Unincorporated biotin-NTPs were removed with RNase free P-30 columns (7326223; Bio-Rad), and three cycles of magnetic MyOne Streptavidin C1 bead purification (65002 ...
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bioRxiv - Cancer Biology 2022Quote: ... The population of cells containing a mix of non-infected and infected cells was then loaded onto an S3e flow cytometer (Bio-Rad). 50,000 cells were analyzed using single-cell gates to measure the median green fluorescence induced by intracellular Fnn ...
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bioRxiv - Physiology 2020Quote: ... conditioned media from cells was passed through columns containing AG1-X8 Anion Exchange Resin (Bio-Rad) and collected in scintillation vials ...
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bioRxiv - Microbiology 2020Quote: ... The cell suspension containing plasmid DNA was electroporated using a Gene Pulser electroporator (Bio-Rad, USA) with the following parameters ...
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bioRxiv - Developmental Biology 2021Quote: ... containing β-mercaptoethanol (BioRad). Boiled samples and protein marker (Bio-rad ...
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bioRxiv - Cancer Biology 2023Quote: ... PVDF membranes were washed three times with tween 20-containing tris buffered saline before incubation with HRP-conjugated secondary antibodies (Bio-Rad). Images were detected using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Biophysics 2021Quote: Cells were mounted on a 2% agarose pad containing resuspension medium using a gene frame (Bio-Rad). Cells were concentrated by centrifugation (3300g for 30 s ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated small RNAs were separated from HPDP-biotin and pyridine-2-thione using spin columns (BioRad) in ultra-pure water.
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bioRxiv - Cell Biology 2022Quote: ... Cell were labelled with rabbit anti-IL-8 antibody (Biorad Biosciences), mouse anti-IL-1β antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit anti-IL-8 antibody (Biorad Biosciences) and mouse anti-IL-1β antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained with anti-BrdU antibody (Bu20a; BioRad, Hercules, CA) and counterstained with secondary Alexa Fluor® 488 conjugated antibody (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2021Quote: ... Guide RNA plasmids were transformed into electrocompetent cells containing the Cas protein using a MicroPulser Electroporator (Bio-Rad). Cells were recovered for 100 min at 37°C in LB broth ...
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bioRxiv - Microbiology 2023Quote: ... the cuvette containing the cell/DNA mixture was inserted into the Bio-Rad GenePulser electroporator (Bio-Rad, Inc), and subjected to an electrical sock using the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... pelleted by centrifugation and resuspended in RPMI medium containing 10% foetal bovine serum where cell count and viability was estimated (BioRad, TC20™ Automated Cell Counter) before plating for experiments.
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bioRxiv - Genetics 2021Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... GFP+ cells were captured into single tube with 100 μl lysis buffer containing Proteinase K and DNase (Bio-Rad). The sorted cells were then incubated for 10 min ...
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bioRxiv - Biophysics 2019Quote: ... containing 5% 2-mercaptoethanol (Bio-Rad), electrophoresed by SDS-PAGE and blotted onto nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 2-merchatoethanol (#1610710, Bio-Rad) and boiling at 95°C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated in primary antibody (rat anti-MBP, BioRad, 1:200 dilution) for 24 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate samples containing 4 μg of protein were fractionated using a 4–20% SDS-PAGE gel (Bio-Rad). Proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: Approximately 1 × 105 bacterial cells were suspended in PBS containing 1% (w/v) PBS-BSA and incubated with fH (Biorad) at a final concentration of 5 μg/mL for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast suspensions (2 × 108 cells/ml) containing 0.6% low melting agarose gel were added to 50-well dispensable mold plates (Bio-Rad). Plugs were immersed in 10 ml of NDS (1% N-lauroyl sarcosinate sodium salt solution ...
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bioRxiv - Physiology 2019Quote: ... individual cardiomyocytes were immediately aspirated and deposited into separate microcentrifuge tubes containing SingleShot Cell Lysis buffer (Bio-Rad, Hercules, CA) supplemented with DNase ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
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bioRxiv - Immunology 2020Quote: ... Excess biotin was removed via size exclusion chromatography using an ENrich SEC 650 10 × 300 mm column (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... including CD44-bio (Miltenyi Cat#130-113-340) and an in-house biotin conjugation (Bio-Rad Laboratories, Cat#LNK262B) of anti-PSGL-1 and mouse IgG1 isotype control (R&D Cat#MAB002 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... containing 2 g Chelex-100 resin (BioRad) to remove divalent metals ...