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Citations for Bio-Rad :
1 - 50 of 52 citations for Vesicular Stomatitis Virus peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The dried peptides were resuspended in deionized (dI) water for peptide estimation using Bradford’s assay (Bio-rad) (44).
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bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
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bioRxiv - Neuroscience 2024Quote: ... The final virus samples were titered by digital droplet PCR (BioRad) using a REP-specific primer/probe set for libraries and a CMV primer/probe set for recombinant virus (Supplementary Table 2) ...
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bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
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bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2021Quote: Concentrated PV or virus was mixed with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Physiology 2022Quote: ... or 16.5% Criterion Tris-Tricine/Peptide gels (Bio-Rad) alongside two protein markers (Precision plus all blue and dual color standards ...
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bioRxiv - Cell Biology 2021Quote: ... peptide was conjugated to Affi-gel resin (Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... (ii) genes in RNA virus infection panel of pre-designed human PrimePCR by BioRad; (iii ...
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bioRxiv - Biochemistry 2020Quote: ... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
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bioRxiv - Biochemistry 2023Quote: ... Tryptic peptides were eluted off with spin columns (BioRad, 7326204) under a vacuum manifold ...
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bioRxiv - Biochemistry 2022Quote: ... The blood was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad) before use in the mosquitoes feeding process ...
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bioRxiv - Immunology 2021Quote: ... and TaqMan Fast Virus 1-step Master Mix) using CFX-96 (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Genetics 2021Quote: ... Dissolved peptide was coupled to Affi-Gel 10 resin (Bio-Rad) that had been washed 3 times in methanol ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated virus was then inactivated and proteins denatured by heating with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and subsequently run on a 4-20% Mini-PROTEAN TGX protein gel (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... Peptide cleavage products were resolved on 10-20% Tris-Tricine gels (BioRad) and RNase 7 samples were resolved on 20% SDS-PAGE gels.
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bioRxiv - Immunology 2023Quote: ... free peptides were removed using a Bio-Spin P-30 column (Biorad), leaving the labeled PTM tetramers in the flowthrough ...
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bioRxiv - Microbiology 2021Quote: Total viral RNA was extracted from 0.1 ml virus stocks using Aurum Total RNA Mini Kit (Bio-Rad) and eluted in 50 μl elution buffer pre-heated to 70°C ...
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bioRxiv - Microbiology 2021Quote: ... All serum samples were confirmed as Dengue virus-positive by means of NS1 diagnostic ELISA test (Platelia, Biorad). Serum samples were filtered using Millipore 0.22 μm PES syringe filters ...
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bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Molecular Biology 2019Quote: ... Eluting peptides were collected for 72 min using a BioFrac fraction collector (Bio-Rad). Fraction collection pattern was set to row and fraction collection size was set to 0.75ml ...
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bioRxiv - Neuroscience 2020Quote: ... and a rabbit anti-vasoactive intestinal peptide (1:500, catalog: 9535-0204, Bio-Rad). The secondary antibodies used in a study were as follows ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with CcdA peptide (residues 46-72) coupled Affi-gel15 (Biorad) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: The specific fluorescence produced during the formation of amplicons/ PCR product from the genomic RNA (converted to cDNA) of Rabies virus was identified by the detector in the qPCR machine (CFX CONNECT, BIORAD). The quantitative result is expressed in terms of relative quantification ...
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bioRxiv - Pathology 2022Quote: ... with an in-house full virus standard for determination of genome loads on a C1000 thermal cycler with the CFX96 Real-Time System (Biorad).
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bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
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bioRxiv - Microbiology 2022Quote: ... Absolute quantifications of virus RNA copies were estimated by modelling a Poisson distribution using QuantaSoft™ analysis software version 1.7 (Bio-Rad). Mean signal from N1 and N2 was extrapolated and expressed as viral RNA copies per microliter of supernatant ...
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bioRxiv - Cancer Biology 2022Quote: ... Virus quantification of KO/activation pool was done by droplet digital PCR (ddPCR) using QX200 Droplet Digital PCR System (Bio-RAD, #1864001).
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bioRxiv - Immunology 2022Quote: Excess Eα peptide was removed with Bio-spin 30 kDa size exclusion columns (Bio-Rad Laboratories) equilibrated with PBS according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10, Biorad) and anti-2A peptide (3H4 ...
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bioRxiv - Immunology 2019Quote: ... The calculation of IgG antibody concentration against each individual influenza virus strain rHA was performed by Bio-Plex Manager™ 6.2 software (Bio-Rad Co., CA).
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bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
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bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Plant Biology 2020Quote: ... Peptide concentration was determined by a modified Lowry procedure using the DC Protein Assay (BioRad; Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Electrophoresis was used to separate peptides in a 16.5% Mini-PROTEAN® Tris-Tricine Gel (Bio-Rad) with tris-tricine (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were transferred to new tubes and peptide concentrations were quantified using the DC Protein Assay (Bio-Rad). Peptides were labeled using 10-plex TMT reagents as described above and 4 μg of proteins were used for each TMT channel ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were transferred to new tubes and peptide concentrations were quantified using the DC Protein Assay (Bio-Rad). 50 μg of each sample were set aside for TMT labeling ...
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bioRxiv - Cell Biology 2021Quote: ... The N-ter (pI 5.5) and C-ter (pI 9.9) peptides were conjugated to Affi-Gel-10 (Bio-rad) agarose beads for affinity-purification ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2 μl of the resuspended peptide was spotted on to a nitrocellulose membrane (BIO-RAD, 0.2 μm #162-0112) and left to air-dry ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins and peptides bearing C-terminal 6xHis tags were detected with mouse anti-6xHis antibody clone AD1.1.10 (BioRad; Cat # MCA1396GA) while detection of glycosylated CRM197-FtO-PS was with anti-F ...
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bioRxiv - Plant Biology 2019Quote: ... Purity and size of each peptide were determined by electrophoresis on a 4-20% Mini-Protean TGX gels (Bio-Rad). The correct mass of each peptide was confirmed by mass spectrometry prior to its use in experiments described below.
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bioRxiv - Immunology 2020Quote: ... Cathepsin B activity was determined using Magic Red™ (a cell-permeable and non-cytotoxic reagent that contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe) and lysosomes were detected with acridine orange (Biorad), according to manufactures instructions.
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bioRxiv - Neuroscience 2021Quote: ... and vasopressin) peptides were spotted onto a 0.2-μm polyvinylidene fluoride membrane (Immun-Blot PVDF Membrane for Protein Blotting, BIO-RAD). The membrane was air dried at room temperature and was washed for 10 min in 0.05 M Tris buffer (pH 7.6 ...
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bioRxiv - Genetics 2023Quote: The expression levels of non-ribosomal peptide synthetase genes were assessed using a real-time amplifier CFX96 (Bio-Rad, USA) and a commercial “PCR-mix SYBR Green I kit” (Syntol ...
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bioRxiv - Bioengineering 2023Quote: We verified the conjugation of peptides to the ICBs following SDS-PAGE under reduced conditions using the Mini-PROTEAN Tetra system (Bio-Rad). The unmodified and peptide-conjugated ICBs were reduced to corresponding heavy and light chains of the Abs by incubating the formulations with 50 mM DTT for 10 min at 70 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were probed for 1 hour at room temperature with 1:500 anti-XLF(New England Peptides, details in Methods under Immunodepletion) or 1:1000 anti-His (Bio-Rad MCA1396A) in PBST with 2.5% BSA ...
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bioRxiv - Plant Biology 2022Quote: ... CLso mature effectors (without signal peptide) were amplified from haplotype A and B infected tomatoes genomic DNA under standard PCR conditions using iProof (Biorad, Supplementary Table 1). Annealing temperatures of primer pairs were calculated using FastPCR (Kalendar et al. ...