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Citations for Bio-Rad :
1 - 50 of 10000+ citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... equipped with 695/55 (Cy5, for visualization of the Precision Plus Protein Standard, Bio-Rad) and 605/50 (Cy3/TAMRA ...
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bioRxiv - Developmental Biology 2023Quote: Whole cell and tissue protein was extracted using the Active Motif protein extraction Kit and quantified with the Bradford assay (Bio-Rad). Snap-frozen cardiac apical tissues were homogenized using Dounce tissue grinder (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... For blotting the SDS-PAGE separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semi-wet transfer (BioRad) and blocked for 1 h with 2% milk ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: For blotting the SDS/PAGE-separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semiwet transfer (Bio-Rad) and blocked for 1 h with PBS/2% milk.
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bioRxiv - Genomics 2021Quote: ... 55 mM 2-mercaptoethanol (BioRad; Cat. No. 1610710), 100 ng/mL M-CSF (Peprotech ...
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing protein (determined by Bradford assay; Biorad) were concentrated using a 10,000 MWCO centrifugal filter (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants containing protein fractions were quantified using the Protein Assay kit (Bio-Rad), with bovine serum albumin used to generate a reference quantification curve.
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bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
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bioRxiv - Molecular Biology 2021Quote: Supernatants containing protein contents were determined by Bradford Protein Assay (Bio-Rad Laboratories, Hercules, CA). Proteins were immunoblotted with anti-PPARα (ab24509 ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing protein were collected and the protein concentration was measured using Bradford dye (BCA, Biorad). The lysate concentration was normalized and Bolt LDS Sample Buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were transferred to a nitrocellulose membrane for 55 min at 15V using a semi-dry electroblotting transfer unit (Bio-Rad, Hercules, CA, United States). Membranes were incubated for 1 hour with blocking buffer (1% dry skim milk ...
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bioRxiv - Plant Biology 2019Quote: ... SDS-PAGE containing proteins were transferred onto PVDF membranes (Bio-Rad) using a semidry transfer cell (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein-containing membranes were incubated in 5% blocking reagent (Bio-Rad) dissolved in 1X TBST (99% Tris buffered saline and 1% Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... The protein lysates containing 20 micrograms of protein were separated in 4-15% polyacrylamide gel (Bio-Rad) with 125 V for 100 min ...
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bioRxiv - Microbiology 2021Quote: ... delemar pyrF mutant (strain M16)55 using the biolistic delivery system (BioRad), and the homogenous transformants were selected on minimal medium lacking uracil11 ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated supernatant containing total cellular protein was then quantified using a DC Protein Assay (Bio-Rad Laboratories) performed as per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... and protein-containing fractions and purity was determined by SDS-PAGE (BioRad). The following day ...
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bioRxiv - Microbiology 2022Quote: ... at 170 V for 55 min using 1x XT-MES buffer (Bio-Rad), transferred to nitrocellulose membranes using the BioRad Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... at 170 V for 55 min using 1x XT-MES buffer (Bio-Rad), transferred to nitrocellulose membranes using the BioRad Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants containing the protein extracts were transferred into fresh 1.5 ml tubes and protein concentration was measured by Bio-Rad Protein Assay Kit using BSA as protein standard (5000002 ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein-antibody complexes were collected by incubating protein extracts with 20 μl SureBeads Protein G magnetic beads (Bio-Rad) for 30 min at room temperature on a rocking shaker ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Labeled EV and cells treated or not with these EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: Cells or EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. 2μg of proteins were electrophoresed on SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... The band containing all proteins was visualized with Biosafe Coomassie G-250 stain (Biorad) and excised from gel ...
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bioRxiv - Physiology 2022Quote: ... The supernatant was mixed with SDS protein sample buffer containing β-mercaptoethanol (Bio-Rad) and resolved by SDS-PAGE on 4-20% Mini-Protean GTX Stain-Free gels (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins in the samples were denatured using sample buffer containing SDS (BioRad, 1610747) and subsequently transferred to a PVDF membrane (Millipore Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-bound DNA was recovered using the Protein A or Protein G magnetic beads (Bio-Rad), washed similarly as DRIP samples and treated with Proteinase K and RNAse after elution ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2023Quote: ... The spun down lysates were measured for total protein using a BCA assay and 15 μg of protein was diluted in 2x Laemmli buffer containing β-mercapto ethanol (Bio-Rad) and heated to 95 °C for 5 mins ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples containing 236 µg protein lysate in final 96 µL of Lysis Buffer using a Bradford protein concentration assay (Bio-Rad 5000006) were then prepared for subsequent reduction and alkylation for 25 min at room temperature by the simultaneous addition of 10X tris(2-carboxyethyl)phosphine hydrochloride (TCEP ...
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bioRxiv - Cancer Biology 2022Quote: ... annealing at 55°C for 30s using CFX Connect Real-Time PCR system (Bio-Rad). The endogenous actin gene (1301 nt of Ov-actin mRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... including unmodified antibodies and a protein ladder (Bio-rad, #1610375) were also used ...
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bioRxiv - Biochemistry 2022Quote: ... Total lysate containing the equivalent of 1.1 mg of protein (Easy Bradford BioRad, BSA standards) was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The mitochondria-containing pellet was resuspended in THE buffer without BSA and the protein concentration measured using the Bradford Protein Assay (Bio-Rad, Munich, Germany). The protein concentration was adjusted to 10 µg/µl with THE buffer.
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bioRxiv - Physiology 2024Quote: ... Primary antibodies were diluted in TBS-T containing 5% dry nonfat milk (Biorad). Membranes were incubated overnight at 4 °C with primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were blocked in PBS containing 5% goat serum (Bio-Rad Antibodies) and 0.1% saponin (SigmaAldrich ...
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bioRxiv - Microbiology 2020Quote: The spots containing the proteins of interest were cut using the EXQuest Spot Cutter (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... The protein sample was diluted with Laemmli buffer containing β-mercaptoethanol (Bio-rad, Hercules, CA, USA) and incubated at 65°C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Cell Biology 2022Quote: ... isoelectric focusing for 55–60 kVh in total was performed in a Protean IEF cell (BioRad, Germany) and the gradient strip was equilibrated in equilibration buffer (6 M urea ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were diluted in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The bound antibodies were detected using luminol containing reagent Clarity Western ECL Substrate (BioRad) and visualized using ChemiDoc imager (BioRad).
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bioRxiv - Microbiology 2021Quote: ... Fractions containing the protein of interest were identified by SDS-PAGE (4-20% precast polyacrylamide gel, BioRad), pooled and concentrated using Vivaspin-PES (cutoff 5 kDa) ...
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bioRxiv - Pathology 2023Quote: Protein samples were denatured using buffer containing Tris-carboxyethyl phosphine hydrochloride (20x XT-Reducing Agent, BioRad-1610792) and separated based on molecular weight by electrophoresis in 4-12% gradient gels (Criterion™ ...
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bioRxiv - Cell Biology 2024Quote: ... Whole lysate samples containing 15-50 μg protein were electrophoresed in 4-15% SDS-PAGE gel (BioRad) and transferred onto nitrocellulose membranes using Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Depending on the primary antibody separated proteins were transferred to nitrocellulose (BioRad) or ...
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bioRxiv - Immunology 2022Quote: ... Antibodies were eluted from Protein G beads in chromatography columns (Bio-Rad) after centrifugation and washing with PBS using 0.1 M glycine (pH=3.0 ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then transferred to fresh low- protein binding tubes and antibody-protein complexes were eluted by incubating in 60 μL 1x Laemmli Sample Buffer (Biorad, cat ...
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bioRxiv - Microbiology 2022Quote: ... flow cytometry analysis was carried out to determine >55% of cells expressing the maturation marker CD35 (E11, Biorad) and >12% of cells expressing the proliferation marker CD71 (DF1513 ...