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Citations for Bio-Rad :
1 - 45 of 45 citations for TRAP 14 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized from pre-TRAP and TRAP RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad, #170-8841). mRNA expression was measured using TaqMan Fast Advanced Master Mix (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Gradient mini-protean gels 8%-14% (Bio-Rad) were used for the western blots ...
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bioRxiv - Microbiology 2022Quote: ... GFP-Trap samples were eluted with 2x Laemmli Buffer (Bio-Rad) prepared with 50mM (DTT ...
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bioRxiv - Bioengineering 2023Quote: ... 14% Acrylamide/Bis Solution 19:1 (Bio-Rad, cat# 1610144), 2.8 g Urea (BioShop ...
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bioRxiv - Microbiology 2022Quote: ... was packaged into a 1.5×14 cm column (Bio-Rad, Carlsbad, CA) and washed with 3 column volumes of molecular-grade H2O and 6 column volumes of binding buffer + denaturing agent ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were separated by 14% SDS-PAGE and transferred to PVDF membranes (Biorad). Following blocking in 5% Milk (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... 2011) and run at 14 °C in a CHEF DR-III apparatus (BioRad) using the field 6V/cm at a 120° angle ...
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bioRxiv - Cancer Biology 2021Quote: ... Electrophoresis was performed at 14 °C in 0.9% pulse field-certified agarose (Bio-Rad) using Tris-borate-EDTA buffer in a Bio-Rad Chef DR III apparatus (9 h ...
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bioRxiv - Neuroscience 2022Quote: Neuronal transfections were carried out at DIV 13–14 using Transfectin (BioRad, Hercules, USA), according to the manufacturers’ instructions (DNA:transfectin ratio 1 µg:3 µl) ...
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bioRxiv - Genetics 2023Quote: ... Cell were counted on day 14 using a TC20 Automated Cell Counter (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2019Quote: ... 120° included angle and 14°C constant temperature in a CHEF-MAPPER system (Bio-Rad) with pulsed times ramped from 0.2s to 120s.
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bioRxiv - Microbiology 2022Quote: ... 120° included angle and 14 °C constant temperature in a CHEF-MAPPER system (Bio-Rad) with pulsed times ranging from 0.5 s to 3 min 10 s with a linear ramping factor.
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated on 14% Tris-Glycine gels and transferred to nitrocellulose membranes (Bio-Rad). Membranes were blocked with 3% milk in Tris-buffered saline with Tween-20 (TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... Myc-5-HT1A-R and eGFP were performed at 13–14 DIV using Transfectin (Bio-Rad), according to the instructions of the manufacturer (DNA/lipofectant ratio of 1:3) ...
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bioRxiv - Bioengineering 2021Quote: ADMSC metabolic activity was tracked over 14 days using the alamarBlue® Cell Viability Assay (Bio-Rad). Media was removed from samples of S-50 or P-0 (n = 5 each) ...
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bioRxiv - Molecular Biology 2021Quote: ... Electrophoresis was carried out in 14 °C cold 0.5x TBE in a CHEF DR-III system (Bio-Rad, initial switch time 60 sec ...
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bioRxiv - Immunology 2021Quote: ... Proteins were separated on 14% polyacrylamide gels and transferred onto nitrocellulose membrane using Trans-blot Turbo (Bio-Rad). Antibodies for immunoblot were against GSDME (EPR19859 ...
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bioRxiv - Neuroscience 2019Quote: ... were multiplexed and diluted to a final concentration of 0.2X and amplified for 14 cycles using a PCR thermal cycler (C1000 Touch, Biorad). After amplification ...
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bioRxiv - Microbiology 2021Quote: ... The mixtures were electrophoresed on 14% SDS PAGE and bocillin fluorescence was imaged using Gel Doc EZ imager (BioRad).
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bioRxiv - Cancer Biology 2021Quote: ... 1 μl of sequin spike (https://www.sequinstandards.com) was added to 14 μl of RNA for cDNA synthesis with SsoAdvanced iScript (Bio-Rad), followed by RT-qPCR using SsoAdvanced master mix (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were run on 14% native polyacrylamide gels in TAE buffer at 4 ° C using a MiniproteanIII system (BioRad) set at 140 V ...
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bioRxiv - Molecular Biology 2023Quote: ... Pulse-field gel electrophoresis was carried out in a 0.9% agarose gel in 0.5× TBE at 14°C with a CHEF DR III from Bio-Rad with a constant switch time of 20 s during 24 h ...
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bioRxiv - Microbiology 2022Quote: ... and trap (primers 41 + 42) transcript levels by RT-qPCR was performed using iQ or iTaq™ Universal SYBR Green supermix (Bio-Rad) and the iQ5 or CFX96 real-time PCR detection systems (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... and switch time linearly ramped from 1 s to 5 s over 14-15 h (CHEF DR III, Bio-Rad). DNA bands were visualized by staining the gel with a solution of 0.5 µg/mL ethidium bromide ...
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bioRxiv - Genetics 2019Quote: ... chromosomal DNA was prepared in agarose plugs as described in (Bani Ismail et al. 2014) and run at 14 °C in a CHEF DR-III apparatus (BioRad) using the field 6V/cm at a 120° angle ...
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bioRxiv - Cell Biology 2022Quote: ... Electrophoresis was performed for 21 h at 14°C in 0.9% (wt/vol) Pulse Field Certified Agarose (Bio-Rad Laboratories) in exactly 2.4L of 0.5x Tris-borate/EDTA buffer in a Rotaphor apparatus (Biometra ...
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bioRxiv - Synthetic Biology 2022Quote: ... PFGE was undertaken by running samples on a 1.0% agarose gel in 0.5 X TBE solution at 14 °C on a Bio-Rad clamped homogeneous electric field apparatus (CHEF-DR III, BioRad). 6 V/cm were used with 15 h switch time of 60 s followed by 15 h 120 s at 120° ...
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bioRxiv - Immunology 2024Quote: ... RNA from 14 3h PBF guts was transcribed into cDNA with the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad) and RT-PCR was performed with RedTaq Polymerase (VWR ...
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bioRxiv - Genetics 2024Quote: ... chromosomal DNA was prepared in 1.3% of low-melting point agarose plugs as described in 74 and run at 14 °C with 1X TBE buffer in a CHEF DR-III apparatus (BioRad) using the field 6V/cm at a 120°angle for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... were then activated with 100% methanol and total protein extracts were transferred for 14 min at 25 V using a Trans-Blot Turbo Transfer System (Bio-Rad). Membrane-bound proteins were visualized with Ponceau S (78376 ...
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bioRxiv - Microbiology 2021Quote: ... Each sample (30 µg) was run on a 14% SDS-PAGE gel and transferred to a nitrocellulose membrane (Bio-Rad, CA). HTLV-1 Gag expression in cells and VLPs in the cell culture supernatant was detected using a mouse monoclonal antibody HTLV-1 p24 (6G9 ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1× TBE buffer at 1.32 V/cm for 14 hr at room temperature with buffer circulation in a Sub-cell GT electrophoresis system (15 × 20 cm gel, Bio-Rad). The gel was stained with 1× TBE buffer containing 0.3 μg/ml of ethidium bromide and photographed ...
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bioRxiv - Biophysics 2021Quote: ... β-galactosidase activity in plates was achieved as previously described 14 with absorbance readings measured on a Benchmark Plus Microplate Reader (Bio-Rad) at 570 nm ...
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bioRxiv - Microbiology 2023Quote: ... in 1X TAE buffer at 14 °C on a Bio-Rad clamped homogeneous electric field apparatus (CHEF-DR III, Bio-Rad). 3 V/cm were used with 96 h switch time of 600 s at 120° ...
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bioRxiv - Genetics 2023Quote: ... Reaction mixtures were resolved on a 14% urea-PAGE gel (AmericanBio, Inc.) and analyzed using a PharosFX molecular imager (Bio-Rad). Single-nucleotide extension products were quantified using Image Lab software ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then clarified through centrifugation (14′000g, 20 min, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Samples were diluted in 5x loading buffer and ∼30 mg run out on a 6-14% SDS-PAGE gel as described before semi-dry Western blotting (BioRad TurboID). Ponceau stained images were taken ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated by SDS-PAGE electrophoresis in 12% or 14% acrylamide gels, and transferred on nitrocellulose membranes (Amersham, 10600021) by semi-dry electrophoretic transfer (Bio-Rad). Accordingly with primary antibody datasheets ...
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bioRxiv - Cell Biology 2020Quote: ... 9 µL of each cell lysate and 14 µL of each secreted protein sample were run on a 4–20% Tris-glycine gel (Bio-Rad; 4561094) together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad) and visualized with GelCode Blue stain (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... Selected siRNAs were spotted onto a gridded MatTek dish (P35G-2-14-C-GRID) using a contact spotter (ChipWriter Pro-Bio-Rad Laboratories) resulting in a layout of 4 × 8 spots 40 ...
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bioRxiv - Biochemistry 2020Quote: ... Samples (14 μL) were loaded into lanes where indicated of an Any kD Mini-PROTEAN TGX 15-well gel (#4569036, Biorad, Hercules CA). Electrophoresis was carried out in native Tris-glycine buffer (25 mM Tris ...
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bioRxiv - Microbiology 2022Quote: We finalized the IPATS-BLV reaction in a 22-μl reaction mixture containing 14 μl of 2× ddPCR Supermix for Probes (Bio-Rad, #1863023), 909 nM of primers except the RPP30 primers (DRB3*016:01-forward ...
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bioRxiv - Cancer Biology 2024Quote: ... Dharmacon, Cambridge, UK) or siRNAs targeting MYC (D-003282-14 & D-003282-35, Dharmacon) using siLentFect transfection reagent (1703362, Biorad, Hercules, CA, USA) (see Table S4 for siRNA sequences) ...
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bioRxiv - Physiology 2022Quote: ... such that a total of 2 μg protein in 20 μL of buffer was loaded into wells of a Mini-PROTEAN TGX Stain-Free gel (5-14%) (Bio-Rad, Mississauga, ON, Canada). Protein ladder (5 μL of Precision Plus Protein™ All Blue Prestained Protein Standards ...