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Citations for Bio-Rad :
1 - 50 of 2439 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The cell pellet was re-suspended in 10mL of fresh culture media (37°C) and cells density was calculated with a TC20 automated cell counter (BioRad). 100’000 to 150’000 cells were plated per 35mm glass-bottom dish and transfected the following day ...
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bioRxiv - Molecular Biology 2021Quote: Cell culture media was exchanged for PBS and the cells were placed in the GS GENE LINKER UV chamber (Bio-Rad). The irradiation was set to 100 mJ for 45 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... fresh culture media with a 1:100 dilution of Alamar blue (Bio-Rad cat. #BUF012A) was then added to the cells for 3 hours ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... cell media was replaced with 200 uL growth assay media (180 uL DME + 20 uL Alamar Blue (BioRad BUF012A), and incubation was continued for 4 hr at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins from cell culture were extracted by scraping the culture wells in the presence of RIPA buffer (BioRad, Hercules, CA) with protease inhibitor and phosphatase inhibitor (St ...
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bioRxiv - Microbiology 2022Quote: ... Cells were imaged 10h post-co-culture using a ZOE Fluorescent Cell Imager (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: Cell culture images were taken every 24 h on Zoe Fluorescent cell imager (Bio-Rad). Three individual images were taken per condition and timepoint ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell density and viability was assessed during culture by a TC20 automated cell counter (Bio-Rad) with trypan blue ...
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bioRxiv - Neuroscience 2021Quote: ... media was replaced with complete media containing siLentfect (Bio-Rad) and siRNA complexes (AllStars Neg ...
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bioRxiv - Bioengineering 2019Quote: ... Klf4 (a marker of stemness [72]) and Hes1 (another known stem cell marker [73]) were measured using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories) according to the manufacturer’s protocols in a Bio-Rad CFX96 Real-Time PCR detection system (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... growth media was removed from cells and cell extracts prepared by lysis in SDS sample buffer (Bio-Rad) supplemented with β-mercaptoethanol (Sigma) ...
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bioRxiv - Immunology 2019Quote: Cytokines in cell culture supernatants were measured by Multiplex assay from Bio-Rad on a Luminex machine following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: Samples from PSB-D suspension culture cells in 1× Laemmli loading buffer (BioRad) with reducing agent (1× NuPage [Invitrogen] or 100 mM DTT ...
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bioRxiv - Biophysics 2023Quote: ... Media was aspirated and cells were immediately lysed with 2x Laemmli Buffer (BioRad 1610737) + 5% BME ...
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bioRxiv - Cancer Biology 2022Quote: ... 2×106 cells (extracted tissues or cell culture) were suspended in 50 μL RIPA (Radio ImmunoPrecipitaion Assay; BioRad) lysis buffer containing 200 mM PMSF (Alpha-Phenyl Methyl Sulfonyl Fluoride ...
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bioRxiv - Plant Biology 2020Quote: ... Stem loop RT-qPCRs were performed in a CFX96 or CFX384 (Bio-Rad) machine using the protocol previously described by[81].
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell culture viability and density were determined with an Automated Cell Counter TC20™ (Bio-Rad Laboratories, Sundyberg, Sweden) by the addition of 0.2% trypan blue (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Cell concentration was determined for three independent cultures of each strain using the TC20 automated cell counter (Bio-Rad). These results were used to normalize the total areas of each chemical feature detected ...
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bioRxiv - Cell Biology 2021Quote: Media collected from T0034 cells were immunoprecipitated with magnetic Protein A beads (Sure Beads, BioRad) as previously described [8] ...
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bioRxiv - Cancer Biology 2019Quote: 150,000 cells were seeded per T25 flask in mNSC media and counted after 72 hours on a TC20 cell counter (Biorad) using Trypan blue stain (Thermo) ...
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bioRxiv - Genetics 2020Quote: ... LSK were plated at day 8 of culture and monitored for growth by counting live cells by Trypan blue exclusion using a TC20TM Automated Cell Couter (BIORAD) at the indicated time ...
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bioRxiv - Cancer Biology 2022Quote: The isolated sEVs and m/lEVs from 1.5 × 106 cells following 72 h of cell culture were lysed with 4X sample buffer (Bio-Rad) with β-mercaptoethanol (for detecting TSG101 and flotillin-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Images of IMR32 cultures after treatments were acquired using a ZOE fluorescent cell imager (Biorad).
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bioRxiv - Neuroscience 2021Quote: ... cell lysates or cell media were subjected to SDS-PAGE using 4-20% gradient Tris-glycine gels (Bio-Rad; Hercules, CA) followed by transfer to Immobilon P membranes (EMD Millipore) ...
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bioRxiv - Biophysics 2024Quote: ... Growth media was exchanged after 24 hours and cells were checked for protein expression using a fluorescent cell imager (Bio-Rad). Cells were harvested after another 24 hours in cold PBS before purification ...
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bioRxiv - Physiology 2020Quote: ... conditioned media from cells was passed through columns containing AG1-X8 Anion Exchange Resin (Bio-Rad) and collected in scintillation vials ...
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bioRxiv - Cell Biology 2022Quote: Images of THP-1 wt and THP-1 RTN1A+ cells in cultures were taken with the ZOE fluorescence cell imager (Bio-Rad). and the numbers of cellular aggregates per 0.7 mm2 from 4 FOV have been quantified using ImageJ Fiji (Fig 5G) ...
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bioRxiv - Cell Biology 2022Quote: ... droplets were viewed on glass slides or in glass-bottom cell culture dishes for droplet and fluorescence imaging using ZOETM Fluorescent Cell Imager (BIO-RAD).
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bioRxiv - Developmental Biology 2021Quote: ... cultures were dissociated with Accutase and analyzed by flow cytometry using an S3 cell sorter (Biorad). Undifferentiated ESCs or iPS cells ...
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bioRxiv - Immunology 2024Quote: Cytokines were measured in cell culture supernatants using luminex analysis (Biorad human 17-plex Bioplex kit) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Concentrated conditioned media normalized to cell numbers were loaded on 4-15 % SDS-PAGE gels (Bio-Rad) and ran at a voltage of 180V under room temperature for electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... 10 µl of normalized cell culture was spotted on 0.2 µm pore-size nitrocellulose membrane (Bio-Rad) and allowed to dry ...
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bioRxiv - Cancer Biology 2020Quote: ... using gene-specific primers for stem-loop qPCR or the iScript cDNA Synthesis Kit (BioRad, 1708890). cDNA was analyzed using SYBR Green Master Mix (BioRad ...
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bioRxiv - Immunology 2020Quote: ... Cytokines from cell culture supernatants were quantified using Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-rad), IFN-β ELISA kit (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... culture supernatants were collected and cells were directly lysed with 150 μl of 2× laemmli buffer (Bio-rad, 1610737). Proteins in culture supernatants and cell lysates were separated by SDS-PAGE and transferred onto PVDF membranes by using the Trans-Blot Turbo system (Bio-rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentrations of culture medium and cell lysate were determined by the Bradford assay (Bio-Rad, Hercules, CA). Culture medium or cell lysate samples were resuspended in SDS sample buffer containing β-mercaptoethanol and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... intermedius overnight cultures were pelleted and resuspended in a 10:1 ratio of culture to InstaGene Matrix (BioRad) after which the extraction protocol as per manufacturer instructions was followed ...
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bioRxiv - Neuroscience 2020Quote: ... Organotypic slices cultures were biolistically transfected (BioRad) at 5-7 DIV and imaged 3-5 days later (Bosch et al. ...
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bioRxiv - Microbiology 2023Quote: ... media were treated with Chelex-100 (chelex: BioRad) as follows ...
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bioRxiv - Immunology 2021Quote: Concentration of 27 inflammation-related human proteins were measured in cell culture supernatants by using Human 27-plex BIORAD cytokine panel (Cat. No.171-A1112, BIORAD). Standard curves were generated for all 27 cytokine standards with eight 2-fold serial dilutions starting from 32 ng/ml ...
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bioRxiv - Genomics 2022Quote: Chromosomes from stationary yeast cell cultures were prepared in agar molds using the Certified Megabase Agarose (Bio-Rad CAT. 1613108) and pulsed-field gel electrophoresis (PFGE ...
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bioRxiv - Cell Biology 2023Quote: Cell growth in liquid media was monitored in a 96-well flat-bottomed plate using a microplate reader (iMark, Bio-Rad). Cells were grown to the mid-log phase and then diluted to OD600 = 0.1 ...
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bioRxiv - Cell Biology 2019Quote: ... IL-10 and MCP-1 in cell culture supernatants at 24 and 96 h post-infection on the Bioplex 200 (Bio-Rad). The Bioplex Manager 6.1 software was used for data analysis (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: Cell cultures and animal tissues: 15 μg of protein extracts were loaded on 10% pre-cast gels (Bio-Rad Cat.# 4568034) and run for 1hr at 100V ...
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bioRxiv - Immunology 2021Quote: ... Cytokine concentrations in cell free culture medium or serum samples were measured using the Bio-Plex Pro Assay System (Bio-Rad Laboratories) [23] ...
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bioRxiv - Immunology 2020Quote: ... Multiplexed cytokine measurements on macrophage cell culture supernatants were performed using a custom Bio-Plex Express Human Cytokine kit (Bio-Rad 17004073) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Pro-inflammatory and pro-fibrotic mediators were measured in 50µl of cell-free tissue culture supernatants using a 37-Plex Bio-Plex Pro™ Human Inflammation Panel 1 (Bio-Rad #171AL001M) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... Bio-Beads (Bio-Beads SM-2 Absorbent Media, Bio-Rad) were added to the reconstitution mixture for 1 hour then fresh Bio-Beads were added and the mixture was allowed to incubate overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Detergent was removed with Bio-beads SM2 adsorbent media (BioRad). Reconstituted sample was recovered from the bio-beads and centrifuged at 10,000 g for 10 minutes at 4°C ...